FATTY ACID BINDING PROTEIN-1 (FABP-1) OF ECHINOCOCCUS GRANULOSUS- A PROMISING VACCINE CANDIDATE- AN IN SILICO ANALYSIS

2016-09-12T01-57-19Z
Source: Indo American Journal of Pharmaceutical Research
Varun Chauhan, Gurjeet Kaur, Naveen, Kapil Goyal, Rakesh Sehgal.
E. granulosus responsible for causing Cystic echinococcosis (CE), a highly pathogenic infection, is causing considerable morbidity and mortality in humans. Fatty Acid Binding Proteins have been suggested to be promising vaccine candidates against several parasitic Platyhelminthes. The present study was thus aimed to identify HLA class I (HLA*A-02:01) restricted T cell epitopes, one of the most common occurring HLA Class I allele in human population, and B cell epitopes by screening of FABP-1 protein of E. granulosus using in-silico approach. The most promising predicted T cell epitopes were docked with HLA-*A 02:01 allele in order to ascertain the binding pattern of the identified peptides and HLA allele. The identified B and T cell epitopes were confirmed by visualizing there locations on the 3D model of FABP-1 protein. We believe that the present study will further help the researchers in better understanding the immune responses generated in an intermediate host in response to FABP protein of the parasite and may provide a platform to facilitate subunit vaccine design.


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