Filling the Void: Proximity-Based Labeling of Proteins in Living Cells

Publication date: Available online 22 September 2016
Source:Trends in Cell Biology
Author(s): Dae In Kim, Kyle J. Roux
There are inherent limitations with traditional methods to study protein behavior or to determine the constituency of proteins in discrete subcellular compartments. In response to these limitations, several methods have recently been developed that use proximity-dependent labeling. By fusing proteins to enzymes that generate reactive molecules, most commonly biotin, proximate proteins are covalently labeled to enable their isolation and identification. In this review we describe current methods for proximity-dependent labeling in living cells and discuss their applications and future use in the study of protein behavior.



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