Impaired Akt phosphorylation in B-cells of patients with common variable immunodeficiency

Publication date: Available online 21 September 2016
Source:Clinical Immunology
Author(s): Reza Yazdani, Mazdak Ganjalikhani-Hakemi, Mohammad Esmaeili, Hassan Abolhassani, Shahram Vaeli, Abbas Rezaei, Zohre Sharifi, Gholamreza Azizi, Nima Rezaei, Asghar Aghamohammadi
Common variable immunodeficiency (CVID) is a heterogeneous group of primary immunodeficiency characterized by recurrent infectious. We evaluated whether defective PI3K/Akt/FoxO pathway could influence B-cell fate. Determination of B-cell subsets in CVD patients and healthy donors (HDs) were performed using flow cytometry. We evaluated mRNA level and protein expression of PI3K, Akt and FoxO molecules using real-time PCR and flow cytometry, respectively. Moreover, phosphorylated Akt (p-Akt) expression in B-cells has also been measured by flowcytometry. We identified a significant reduction in percentage of marginal zone like B-cells, Switched and unswitched memory B-cells, total memory B-cells and plasmablasts in patients, as these reductions of had significantly negative correlation with increased apoptosis in patients. Surprisingly, we identified decreased p-AKT expression in B-cells of patients than HDs. We describe for the first time impaired p-Akt expression in B-cells of CVID patients that had a significant correlation with antibody response to vaccine and clinical manifestations.



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