Ετικέτες

Πέμπτη 23 Μαρτίου 2017

Dendritic Cells Display Subset and Tissue-Specific Maturation Dynamics over Human Life

Publication date: 21 March 2017
Source:Immunity, Volume 46, Issue 3
Author(s): Tomer Granot, Takashi Senda, Dustin J. Carpenter, Nobuhide Matsuoka, Joshua Weiner, Claire L. Gordon, Michelle Miron, Brahma V. Kumar, Adam Griesemer, Siu-Hong Ho, Harvey Lerner, Joseph J.C. Thome, Thomas Connors, Boris Reizis, Donna L. Farber
Maturation and migration to lymph nodes (LNs) constitutes a central paradigm in conventional dendritic cell (cDC) biology but remains poorly defined in humans. Using our organ donor tissue resource, we analyzed cDC subset distribution, maturation, and migration in mucosal tissues (lungs, intestines), associated lymph nodes (LNs), and other lymphoid sites from 78 individuals ranging from less than 1 year to 93 years of age. The distribution of cDC1 (CD141hiCD13hi) and cDC2 (Sirp-α+CD1c+) subsets was a function of tissue site and was conserved between donors. We identified cDC2 as the major mature (HLA-DRhi) subset in LNs with the highest frequency in lung-draining LNs. Mature cDC2 in mucosal-draining LNs expressed tissue-specific markers derived from the paired mucosal site, reflecting their tissue-migratory origin. These distribution and maturation patterns were largely maintained throughout life, with site-specific variations. Our findings provide evidence for localized DC tissue surveillance and reveal a lifelong division of labor between DC subsets, with cDC2 functioning as guardians of the mucosa.

Graphical abstract

image

Teaser

Dendritic cells (DCs) function as tissue sentinels, but this role is difficult to study in humans. In this issue of Immunity, Granot et al. show through analysis of lymphoid and mucosal tissues that human DC maturation is tissue specific, associated with migration phenotypes, and predominantly observed among the cDC2 subset.


http://ift.tt/2nJe5WF

Δεν υπάρχουν σχόλια:

Δημοσίευση σχολίου

Αναζήτηση αυτού του ιστολογίου