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Πέμπτη 13 Απριλίου 2017

Label-free upconversion nanoparticles-based fluorescent probes for sequential sensing of Cu2+, pyrophosphate and alkaline phosphatase activity

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Publication date: 15 September 2017
Source:Biosensors and Bioelectronics, Volume 95
Author(s): Fangfang Wang, Cuiling Zhang, Qin Xue, Huaping Li, Yuezhong Xian
An efficient near-infrared fluorescence probe has been developed for the sequential detection of Cu2+, pyrophosphate (P2O74−, PPi), and alkaline phosphatase (ALP), which is based on the "off−on−off" fluorescence switch of branched polyethyleneimine (PEI)-capped NaGdF4:Yb/Tm upconversion nanoparticles (UCNPs). The fluorescence is quenched via energy transfer from UCNPs to Cu2+ for the coordination of PEI with Cu2+. The strong affinity between Cu2+ and PPi leads to the formation of Cu2+-PPi complex and results in the detachment of Cu2+ from the surface of UCNPs, thus the fluorescence is triggered on. ALP-directed hydrolysis of PPi causes the disassembly of Cu2+-PPi complex and re-conjugation between Cu2+ with PEI, which leads to the switch-off fluorescence of UCNPs. The system allows sequential analysis of Cu2+, PPi, and ALP by modulating the switch of the fluorescence of UCNPs with detection limits of 57.8nM, 184nM, and 0.019U/mL for Cu2+, PPi, and ALP, respectively. By virtue of the NIR feature and excellent biocompatibility, the UCNPs-based probes are suitable for bioimaging. Taking Cu2+ visualization as a model, the nanoprobes have been successfully applied for intracellular imaging of Cu2+ in living cells.



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