P 254 Towards Magnetic Mapping of Cellular Organelles using Fluorescent Nanodiamonds

Publication date: July 2017
Source:Free Radical Biology and Medicine, Volume 108, Supplement 1
Author(s): Simon Robert Hemelaar, K.J. van der Laan, Romana Schirhagl
Recently, Fluorescent NanoDiamonds (FNDs) have gained attention in the physical, chemical and biological fields. Their fluorescence is influenced by the magnetic surrounding. This allows magnetic resonance imaging on the nanoscale. Since the read out is optical only a microscope is needed and the technique is so sensitive that even single electron spins are visible.We show that nanodiamonds are non-toxic in mammalian cell lines, which freely take up these particles. In addition, we show the effects of different concentrations, size, surface termination, shape and aggregation state on the biocompatibility at the level of genetic and protein changes as well as overall viability and reactive oxygen species (ROS) production.Yeast cells can be stimulated to ingest FNDs after chemical or electrical transformation. Altogether, Fluorescent Nanodiamonds show much promise to become a sensitive and direct detector for a wide range of molecules, among which free radicals are a major contender because of their high magnetic moment.In order to understand a spectrum from the interior of a cell, we isolate organelles and analyze these using our highly sensitive homebuilt confocal setup. In addition, we target cell organelles using an antibody targeting with biotin-streptavidin interactions. Here we present the results of our pilot experiments and give an overview of single organelle magnetometry implications.



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