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Τρίτη 18 Ιουλίου 2017

Proteomic and Metabolomic Characterization of a Mammalian Cellular Transition from Quiescence to Proliferation

Publication date: 18 July 2017
Source:Cell Reports, Volume 20, Issue 3
Author(s): Ho-Joon Lee, Mark P. Jedrychowski, Arunachalam Vinayagam, Ning Wu, Ng Shyh-Chang, Yanhui Hu, Chua Min-Wen, Jodene K. Moore, John M. Asara, Costas A. Lyssiotis, Norbert Perrimon, Steven P. Gygi, Lewis C. Cantley, Marc W. Kirschner
There exist similarities and differences in metabolism and physiology between normal proliferative cells and tumor cells. Once a cell enters the cell cycle, metabolic machinery is engaged to facilitate various processes. The kinetics and regulation of these metabolic changes have not been properly evaluated. To correlate the orchestration of these processes with the cell cycle, we analyzed the transition from quiescence to proliferation of a non-malignant murine pro-B lymphocyte cell line in response to IL-3. Using multiplex mass-spectrometry-based proteomics, we show that the transition to proliferation shares features generally attributed to cancer cells: upregulation of glycolysis, lipid metabolism, amino-acid synthesis, and nucleotide synthesis and downregulation of oxidative phosphorylation and the urea cycle. Furthermore, metabolomic profiling of this transition reveals similarities to cancer-related metabolic pathways. In particular, we find that methionine is consumed at a higher rate than that of other essential amino acids, with a potential link to maintenance of the epigenome.

Graphical abstract

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Teaser

Lee et al. generate and integrate quantitative time-course proteomic and metabolomic profiling data to find that global metabolic reprogramming of an IL-3 activation resembles metabolic rewiring in cancer with high consumption of methionine in G1.


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