Resolving the Gordian Knot: Srs2 Strips Intermediates Formed during Homologous Recombination

Publication date: Available online 28 December 2017
Source:Trends in Biochemical Sciences
Author(s): Harshad Ghodke, Jacob S. Lewis, Antoine M. van Oijen
Cells use a suite of specialized enzymes to repair chromosomal double-strand breaks (DSBs). Two recent studies describe how single-molecule fluorescence imaging techniques are used in the direct visualization of some of the key molecular steps involved. De Tullio et al. and Kaniecki et al. watch individual Srs2 helicase molecules disrupt repair intermediates formed by RPA, Rad51, and Rad52 on DNA during homologous recombination.



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