Ετικέτες

Σάββατο 24 Φεβρουαρίου 2018

Spine Patterning Is Guided by Segmentation of the Notochord Sheath

Publication date: 20 February 2018
Source:Cell Reports, Volume 22, Issue 8
Author(s): Susan Wopat, Jennifer Bagwell, Kaelyn D. Sumigray, Amy L. Dickson, Leonie F.A. Huitema, Kenneth D. Poss, Stefan Schulte-Merker, Michel Bagnat
The spine is a segmented axial structure made of alternating vertebral bodies (centra) and intervertebral discs (IVDs) assembled around the notochord. Here, we show that, prior to centra formation, the outer epithelial cell layer of the zebrafish notochord, the sheath, segments into alternating domains corresponding to the prospective centra and IVD areas. This process occurs sequentially in an anteroposterior direction via the activation of Notch signaling in alternating segments of the sheath, which transition from cartilaginous to mineralizing domains. Subsequently, osteoblasts are recruited to the mineralized domains of the notochord sheath to form mature centra. Tissue-specific manipulation of Notch signaling in sheath cells produces notochord segmentation defects that are mirrored in the spine. Together, our findings demonstrate that notochord sheath segmentation provides a template for vertebral patterning in the zebrafish spine.

Graphical abstract

image

Teaser

Wopat et al. show that the outer layer of the zebrafish notochord, the sheath, segments into alternating mineralizing and cartilage-like domains prior to vertebral body formation. Mineralized sheath domains, patterned by the segmented activation of Notch, then recruit osteoblasts to form vertebral bodies. Thus, the notochord instructs spine patterning.


http://ift.tt/2GHb92l

Δεν υπάρχουν σχόλια:

Δημοσίευση σχολίου

Αναζήτηση αυτού του ιστολογίου