Photoelectrochemical platform for cancer cell glutathione detection based on polyaniline and nanoMoS2 composites modified gold electrode

Publication date: 30 July 2018
Source:Biosensors and Bioelectronics, Volume 112
Author(s): Xu Hun, Yanxin Li, Shiyu Wang, Yao Li, Jikuan Zhao, Hui Zhang, Xiliang Luo
Herein, a visible light photoelectrochemical (PEC) platform based on polyaniline (PANI) and nanoMoS2 composites as optoelectronic material for glutathione detection without any auxiliary of biomolecules or labeled materials was developed. Firstly, the nanoMoS2 was prepared via a simple ultrasound exfoliation method. The PANI was synthesized by chemical oxidative polymerization method. Then composite of PANI and nanoMoS2 was used to modify gold electrode. It was found that the composite membrane showed excellent PEC properties. And glutathione enhanced the PEC signal greatly. Based on this finding a method for glutathione detection was fabricated. Under the optimum conditions, the linear response of glutathione concentrations ranged from 1.0 × 10⁻¹⁰ to 1.0 × 10⁻⁴ mol L⁻¹ was obtained with a detection limit of 3.1 × 10⁻¹¹ mol L⁻¹. The relative standard deviation was 2.9% at 2.0 × 10⁻⁹ M (n = 10). This method showed high sensitivity and simpleness which opened up a new promising signal-on PEC platform for future bioassay.



https://ift.tt/2HKQQ8p