Proteomic analysis reveals that pheophorbide a-mediated photodynamic treatment inhibits prostate cancer growth by hampering GDP-GTP exchange of ras-family proteins

Publication date: Available online 22 May 2018
Source:Photodiagnosis and Photodynamic Therapy
Author(s): Dan Dan Xu, Chong Bing Xu, Hon Ming Lam, Fuk-Ling Wong, Albert Wing Nang Leung, Merrin Man Long Leong, William Chi Shing Cho, Robin Hoeven, Lv Qing Tao, Rong Rong
BackgroundWe previously reported that pheophorbide a (PhA), excited by 630 nm light, significantly inhibited the growth of prostate cancer cells. In this study, we employed whole-cell proteomics to investigate photodynamic treatment (PDT)-related proteins.MethodsTwo-dimensional gel electrophoresis (2-DE) coupled with tandem mass spectrometry was employed to reveal the proteins involved in PhA-mediated PDT in LNCaP and PC-3 prostate cancer cells.ResultsAfter PhA-PDT treatment, decreased expression of translationally-controlled tumor protein (TCTP) was found in both PC-3 and LNCaP whole-cell proteomes. In contrast, human rab GDP dissociation inhibitor (GDI) in LNCaP cells and ras-related homologs GDI in PC-3 cells were up-regulated.ConclusionsGDP-GTP exchange is an underlying target of photodynamic treatment in prostate cancer cells.



https://ift.tt/2IDv6Nd