Food Allergens: An Update on Analytical Methods

Publication date: 2018
Source:Reference Module in Food Science
Author(s): Michael J. Walker
Food allergy is an increasing public health problem, particularly in developed nations. This article discusses the nature of food allergy, the risk management strategies adopted by regulators and the need for allergen analysis. An overview is given of the different approaches to allergen analysis currently adopted. The article concentrates on enzyme linked immunosorbent assay, ELISA, since this is currently the technique most applied in routine allergen analysis. The history and main formats (sandwich and direct ELISAs) are described along with practical advice on extraction, validation and reporting results. Research proteomics, polymerase chain reaction for amplification and detection of allergen species DNA and liquid chromatography-mass spectrometry approaches are described.



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