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Σάββατο 25 Αυγούστου 2018

Isolation and characterization of phosphate solubilizing bacteria from rhizosphere soils of the Yeyahu Wetland in Beijing, China

Abstract

Phosphate solubilizing bacteria (PSB) can convert insoluble forms of phosphorus (P) to accessible forms. 11 strains of PSB, including five inorganic phosphate solubilizing bacteria (IPSBs) and six organic phosphate solubilizing bacteria (OPSBs), were isolated from rhizosphere soils of three plants Scirpus planiculmis, Zizania latifolia, and Phrnagmites australis in the Yeyahu Wetland of Beijing, China to investigate P-solubilizing activities. In addition, the distributions of P fractions in soil samples were also observed. All strains evaluated above 1.0 by the ratio of transparent circle diameter to colony diameter (D/d) on Ca3(PO4)2 or lecithin plates were identified by 16S rRNA sequencing. Results showed that Ca-bound P (Ca-P) was the main species of inorganic P (IP), and highly resistant organic P (HR-OP) accounted for the most part of organic P (OP). These strains were identified as bacterial species of Enterobacter asburiae, Acinetobacter sp., Bacillus cereus strain, and so on. The most efficient IPSB strain could convert over 430 mg L−1 orthophosphate, while the equivalent OPSB strain only liberated less than 4 mg L−1 in liquid culture, which indicated that IPSBs have a better P-solubilizing ability than OPSBs in rhizosphere soils of the Yeyahu Wetland and IPSBs are likely to regulate the P transformation process in this wetland.

Graphical abstract



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