Correlation of microvessel parameters in invasive ductal carcinoma breast and fibroadenomas: a morphometric study

Publication date: Available online 27 September 2016
Source:Annals of Diagnostic Pathology
Author(s): Dibyajyoti Boruah, Jasvinder K Bhatia, Abhishek Rai, V Srinivas, V S Nijhawan
IntroductionModifications of microvascular configuration are essential features encountered during the progression of breast tumours. Our objectives were to correlate morphometrically evaluated microvessel parameters [microvessel density(MVD), microvessel caliber(VC), microvessel cross sectional area(VCSA), percentage of total VCSA (%TVCSA) and total microvessel boundary density(TVBD)] with histological grades of invasive ductal carcinoma breast(IDC) and benign breast lesions.Materials and MethodsSixty cases of IDC presented with modified radical mastectomy and 20 benign breast fibro-adenomas were evaluated for various microvessel parameters, using CD34-immunostained histological sections by computerized image morphometry. Samples were divided into four histological groups: Benign, Grade1, Grade2 and Grade3; mean with standard deviation and range was evaluated for each group.ResultsHistological grades showed strong positive correlation with %TVCSA(ρ=0.773) and TVBD(ρ=0.811), moderate positive correlation with MVD(ρ=0.607), VC(ρ=0.609) and VCSA(ρ=0.616) when analyzed for all samples of the four groups. Except MVD, all parameters including age was the lowest (P<.001) for the benign group. Among the IDCs, differences of mean VC and VCSA were not significant; MVD, %TVCSA and TVBD were the lowest in Grade1 and the highest in Grade3. Upper cutoff values of benign lesions for: MVD was 155mm⁻², VC was 9.94μm, VCSA was 94.42μm², %TVCSA was 1.33 and TVBD was 4.37mm⁻¹.ConclusionTVBD included the information of microvessels concentration and size showed the best correlation with grades. MVD showed positive correlation with grades in the IDCs; but for the differentiation of benign from malignant, VC, VCSA, %TVCSA and TVBD showed excellent area under the ROC curve (AUC>0.990), unlike MVD (AUC=0.797).



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