GATA3 expression in clinically useful groups of breast carcinoma: a comparison with GCDFP15 and mammaglobin for identifying paired primary and metastatic tumors

Publication date: Available online 26 September 2016
Source:Annals of Diagnostic Pathology
Author(s): Yu-Qiong Yang, Shan-Ming Lu, Wen-Qin Zeng, Shou-Cheng Xie, Sheng-Jun Xiao
GATA3 has been recognized as the novel marker for identifying primary and metastatic breast carcinomas, consistently showing that GATA3 was significantly more sensitive than traditional marker GCDFP15 and Mammaglobin (MGB). However, clinically useful groups of breast carcinomas status were not identified, which was determining appropriate treatment strategy, affecting the prognosis. In this study, we undertook a comparative study of the marker GATA3 and GCDFP15 and MGB in clinically useful groups of paired primary and metastatic breast cancer. We retrieved 64 cases of matched primary and metastatic breast cancer from the surgical pathology archive at our institution. According to the emerging St. Gallen 2015 consensus, the clinically useful groups were divided into ER and/or PR (+), HER2 (−), abbreviated as A; ER and/or PR (+), HER2 (+), abbreviated as B; ER and PR (−), HER2 (+), abbreviated as C; ER, PR and HER2 (−), abbreviated as D; each group contained 16 cases (n=16). Tissue microarrays were created, with three 1-mm punch specimens from each case. The tissue microarrays were cut at a 4-μm thickness and stained with monoclonal antibodies to GATA3, GCDFP15 and MGB. Staining intensity (0~3+) and extent (0% to 100%) were scored with an H-score calculated (range, 0 to 300). Sensitivities by varying H-score cutoffs (any; ≥50; ≥150) for a positive result in the clinically useful groups of matched primary or metastatic breast cancer among GATA3, GCDFP15 and MGB. GATA3 was significantly more sensitive than GCDFP15 and MGB in a majority of paired primary or metastatic breast cancer groups, including all groups, A and B groups (P<.05) rather than C and D groups (P>.05). However, GATA3 in conjunction with GCDFP15 and MGB detection could improve the sensitivity of C group (P<.05) rather than D group (P>.05). Significantly, good coincidence was observed between primary and metastatic tumor GATA3 expression (kappa value =0.826>0.75) as compared with the coincidence of GCDFP15 (kappa value =0.492<0.75) and MGB (kappa value =0.593<0.75) (both P<.05). In conclusion, GATA3 expression did not show the same sensitivity for the clinically useful groups of breast cancer. GATA3 expression is positively correlated with ER-positive, PR-positive, and HER2-positive carcinomas. In addition, the matched primary and metastatic tumor expression of GATA3 show good coincidence. We propose the careful selection of GATA3 for identifying hormone receptor-negativity of breast cancer, especially in the case of triple-negative breast cancer.



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