Reference datasets of tufA and UPA markers to identify algae in metabarcoding surveys

Publication date: Available online 13 February 2017
Source:Data in Brief
Author(s): Vanessa Rossetto Marcelino, Heroen Verbruggen
The data presented here are related to the research article "Multi-marker metabarcoding of coral skeletons reveals a rich microbiome and diverse evolutionary origins of endolithic algae" [1]. Here we provide reference datasets of the elongation factor Tu (tufA) and the Universal Plastid Amplicon (UPA) markers in a format that is ready-to-use in the QIIME pipeline [2]. In addition to sequences previously available in GenBank, we included newly discovered endolithic algae lineages using both amplicon sequencing [1] and chloroplast genome data [3, 4]. We also provide a script to convert GenBank flatfiles into reference datasets that can be used with other markers. The tufA and UPA reference datasets are made publicly available here to facilitate biodiversity assessments of microalgal communities.



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