A novel model for cutaneous wound healing and scarring in the rat

Background: Current rodent models of wound healing and scarring are flawed due to rapid wound contraction and inconspicuous scarring after healing, which is not closely parallel to the physiological process in human. This study was designed to establish a novel model of wound healing and scarring in rats. Methods: Excisional wounds were generated in rat tail or dorsal skin and assessed in terms of histological changes and wound contraction 2, 10 and 16 days postinjury. After healing, rat tail scar was investigated for 24 consecutive weeks by histological and immunohistochemical (IHC) staining. Finally, a stretched scar model was generated in rat tail with high or low strain after re-epithelialization to mimic human hypertrophic scar (HS). The tail HS were subsequently analyzed by histological, IHC staining and mRNA quantification 0, 2, 6, 12 and 24 weeks after stretching. Results: Compared with the dorsal wounds, a larger dermal gap percentage (p

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