Muscle-Derived Stem Cell Enriched Scaffolds Are Capable Of Enhanced Healing Of A Murine Volumetric Muscle Loss Defect

BACKGROUND: Volumetric muscle loss (VML) secondary to traumatic or surgical etiologies can lead to functional and aesthetic impairments. We hypothesize that an implantable muscle-derived stem cell (MDSC) enriched collagen scaffold could significantly augment muscle regeneration in a murine model of VML. METHODS: Murine MDSC were isolated using a modified pre-plating technique and seeded onto type-1 collagen scaffolds to create the MDSC-enriched collagen scaffolds (M-CS). Murine rectus femoris defects of 5mm were created and randomized to one of three conditions (n=6/group): untreated controls, collagen scaffold only, and M-CS. In vivo muscle healing was quantified using microCT. Muscle explants were analyzed using standard histology and whole mount immunofluorescence (IF) at 8 weeks. RESULTS: In vivo experiments demonstrated significantly greater quadriceps cross-sectional area (CSA) in the M-CS group compared to controls on microCT (0.74±0.21 vs. 0.55±0.06 vs 0.49±0.04 ratio of experimental to naïve quadriceps CSA, p

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