MiR-1260b promotes the migration and invasion in non-small cell lung cancer via targeting PTPRK

Publication date: Available online 14 February 2018
Source:Pathology - Research and Practice
Author(s): Limin Xu, Xuting Xu, Huilian Huang, Zhihong Ma, Shuangmei Zhang, Pingping Niu, Yingrong Chen, Jinliang Ping, Ping Lu, Caihua Yu, Lishan Min, Jing Chen, Licheng Dai, Shunli Dong
ObjectiveNon-small cell lung cancer (NSCLC) accounts for 80-85% of lung cancer cases which cause most of cancer-related deaths globally. As our previous study discovered miR-1260b can be regarded as a specific signature for metastasis in NSCLC patients. However, the molecular mechanisms of miR-1260b underlying NSCLC progression and metastasis remain dismal.MethodsThe expression of miR-1260b in NSCLC tissues and cell lines were examined by real-time PCR, the effects of miR-1260b on cell migration, invasion and proliferation were evaluated in vitro. Furthermore, luciferase reporter assay was performed to identify the targets of miR-1260b, and the association between miR-1260b and its target gene was determined by real-time PCR and western blot assay.ResultsThe results showed that miR-1260b was significantly upregulated in NSCLC cell lines. The inhibition of miR-1260b expression decreased the migratory and invasive rates in A549 cells while miR-1260b overexpression had the opposite effect. Furthermore, PTPRK was identified as a direct target of miR-1260b, and PTPRK expression was inversely correlated with miR-1260b in NSCLC cell lines and clinical tissues.ConclusionsThese results suggested that miR-1260b may play an important role in NSCLC metastasis progression and could serve as a putative target for diagnosis and treatment of NSCLC.



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