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Τετάρτη 19 Ιουλίου 2017

Nuclear Pore Complexes: A Scaffold Regulating Developmental Transcription?

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Publication date: Available online 19 July 2017
Source:Trends in Cell Biology
Author(s): Atsushi Satomura, Jason H. Brickner
Nuclear pore complexes (NPCs) have a conserved, but poorly understood, role in transcriptional regulation. Recently, in Developmental Cell, Raices et al. argued that tissue-specific nuclear pore proteins (Nups) act as scaffolds that recruit the transcription factor Mef2C to the NPC, promoting transcription of NPC-associated genes during muscle development.



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Metabolic Interactions in the Tumor Microenvironment

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Publication date: Available online 19 July 2017
Source:Trends in Cell Biology
Author(s): Costas A. Lyssiotis, Alec C. Kimmelman
Tumors are dynamic pseudoorgans that contain numerous cell types interacting to create a unique physiology. Within this network, the malignant cells encounter many challenges and rewire their metabolic properties accordingly. Such changes can be experienced and executed autonomously or through interaction with other cells in the tumor. The focus of this review is on the remodeling of the tumor microenvironment that leads to pathophysiologic interactions that are influenced and shaped by metabolism. They include symbiotic nutrient sharing, nutrient competition, and the role of metabolites as signaling molecules. Examples of such processes abound in normal organismal physiology, and such heterocellular metabolic interactions are repurposed to support tumor metabolism and growth. The importance and ubiquity of these processes are just beginning to be realized, and insights into their role in tumor development and progression are being used to design new drug targets and cancer therapies.



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Does professional experience save teeth? A survey among prosthodontists

Abstract

Objectives

The aim of this study is to investigate the effect of clinical experience and educational background on clinical decision-making of dentists presented with two prosthodontic cases.

Methods

A questionnaire-based survey was performed among 66 prosthodontists who were divided into two groups. The first group included clinicians from Belgium, Germany, and the Netherlands covering a wide spectrum of dental experience. The second (specialized) group consisted of German dentists performing their last module of a 3-year postgraduate master program in prosthodontics. Two patient scenarios of different complexities were presented to the participants who were asked to make an educated choice among seven therapeutic options.

Results

Sixty-six dentists participated. For case #1, 22 therapeutic suggestions were offered within the international group (variation 54%), while 17 options were chosen in the specialized group (variation 68%). For case #2, five (variation 12%) and four (variation 16%) different therapeutic options were planned in the groups, respectively. Treatment planning in combination with implants was clearly preferred by dentists with an experience of 20 years or less. Conversely, participants with more experience favored more conventional treatments. All differences were statistically significant (p < 0.05).

Conclusion

Clinicians' experience and educational background had a clinically relevant influence on decision-making and treatment planning. A clear preference for implant-free therapies was noted for dentists with more than two decades of professional experience.

Clinical significance

In complex cases, clinicians with more than 20 years of professional experience tend to save questionable teeth rather than to resort to implants. This interindividual variation might less likely occur when more external evidence is taken into account during the decision-making process.



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Photofunctionalization and non-thermal plasma activation of titanium surfaces

Abstract

Objective

The aim of this study was to compare UV light and non-thermal plasma (NTP) treatment regarding the improvement of physical material characteristics and cell reaction on titanium surfaces in vitro after short-term functionalization.

Materials and methods

Moderately rough (Ra 1.8–2.0 μm) sandblasted and acid-etched titanium disks were treated by UV light (0.05 mW/cm2 at λ = 360 nm and 2 mW/cm2 at λ = 250 nm) or by NTP (24 W, -0.5 mbar) of argon or oxygen for 12 min each. Surface structure was investigated by scanning electron microscopy, confocal microscopy and X-ray photoelectron spectroscopy (XPS). Hydrophilicity was assessed by dynamic contact angle measurement. Cell attachment, viability, cell proliferation and cytotoxicity were assessed in vitro using murine osteoblast-like cells.

Results

UV irradiation or NTP treatment of titanium surfaces did not alter the surface structure. XPS analysis revealed a significantly increased oxidation of the surface and a decrease of carbon after the use of either method. NTP and UV light led to a significant better cell attachment of murine osteoblasts; significantly more osteoblasts grew on the treated surfaces at each time point (p < 0.001).

Conclusions

UV light as well as NTP modified the surface of titanium and significantly improved the conditions for murine osteoblast cells in vitro. However, results indicate a slight advantage for NTP of argon and oxygen in a short time interval of surface functionalization compared to UV.

Clinical relevance

UV light and NTP are able to improve surface conditions of dental implants made of titanium.



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Subacute cutaneous lupus erythematosus induced by nivolumab



http://onlinelibrary.wiley.com/resolve/doi?DOI=10.1111%2Fajd.12681

Dermoscopy is useful for the diagnosis of milia-like idiopathic calcinosis cutis



http://onlinelibrary.wiley.com/resolve/doi?DOI=10.1111%2Fajd.12675

Survey of Australian dermatologists’ perceptions and experiences with isotretinoin-induced depression and suicide



http://onlinelibrary.wiley.com/resolve/doi?DOI=10.1111%2Fajd.12602

Eczema exacerbation caused by Coxsackie virus A6



http://onlinelibrary.wiley.com/resolve/doi?DOI=10.1111%2Fajd.12677

Sorafenib-induced erythema annulare centrifugum with spontaneous resolution after skin biopsy



http://onlinelibrary.wiley.com/resolve/doi?DOI=10.1111%2Fajd.12623

Dermoscopy of pigmented invasive ductal carcinoma mimicking basal cell carcinoma



http://onlinelibrary.wiley.com/resolve/doi?DOI=10.1111%2Fajd.12671

Mohs micrographic surgery using paraffin sections for the treatment of dermatofibroma of the face: A preliminary case series



http://onlinelibrary.wiley.com/resolve/doi?DOI=10.1111%2Fajd.12601

Facial annular elastolytic giant cell granuloma treated with pulsed dye laser and intralesional polydeoxyribonucleotide



http://onlinelibrary.wiley.com/resolve/doi?DOI=10.1111%2Fajd.12688

Localised longitudinal erythronychia caused by subungual capillary hemangioma



http://onlinelibrary.wiley.com/resolve/doi?DOI=10.1111%2Fajd.12576

Fixed drug eruption probably induced by azithromycin



http://onlinelibrary.wiley.com/resolve/doi?DOI=10.1111%2Fajd.12563

Seasonal variation of chemical composition and source apportionment of PM 2.5 in Pune, India

Abstract

Particulate matter with size less than or equal to 2.5 μm (PM2.5) samples were collected from an urban site Pune, India, during April 2015 to April 2016. The samples were analyzed for various chemical constituents, including water soluble inorganic ions, organic carbon (OC), and elemental carbon (EC). The yearly mean total mass concentration of PM2.5 at Pune was 37.3 μg/m3, which is almost four times higher than the annual WHO standard (10 μg/m3), and almost equal to that recommended by the Central Pollution Control Board, India (40 μg/m3). Measured (OC, EC) and estimated organic matter (OM) were the dominant component (56 ± 11%) in the total particulate matter which play major role in the regional atmospheric chemistry. Total measured inorganic components formed about 35% of PM2.5. Major chemical contributors to PM2.5 mass were OC (30%), SO42− (13%), and Cl and EC (9% each). The high ratios of OC/EC demonstrated the existence of secondary organic carbon. The air mass origin and correlations between the various components indicate that long range transport of pollutants from Indo-Gangetic Plain (IGP) and Southern part of the Arabian Peninsula might have contributed to the high aerosol mass during the dry and winter seasons. To our knowledge, this is the first systematic study that comprehensively explores the chemical characterization and source apportionment of PM2.5 aerosol speciation in Pune by applying multiple approaches based on a seasonal perspective. This study is broadly applicable to understanding the differences in anthropogenic and natural sources in the urban environment of particle air pollution over this region.



http://link.springer.com/10.1007/s11356-017-9761-3

Atypical responses in patients with advanced melanoma, lung cancer, renal-cell carcinoma and other solid tumors treated with anti-PD-1 drugs: A Systematic Review

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Publication date: Available online 19 July 2017
Source:Cancer Treatment Reviews
Author(s): Paola Queirolo, Francesco Spagnolo
Anti-programmed death receptor 1 (PD-1) drugs nivolumab and pembrolizumab were recently approved for the treatment of advanced melanoma and other solid tumors. Atypical patterns of response (i.e. tumor shrinkage or stabilization after initial progression) were observed in about 10% of metastatic melanoma patients treated with anti-cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) drug ipilimumab and were associated with improved survival; however, the rate of atypical response patterns to anti-PD-1 therapy is not clear. An electronic search was performed to identify clinical trials evaluating response to anti-PD-1 therapy with nivolumab and pembrolizumab in patients with advanced solid tumors.Thirty-eight studies were included in our analysis for a total of 7069 patients with advanced cancer treated with anti-PD-1 therapy. Responses were evaluated by unconventional response criteria in 19 trials and were observed for all cancer types but tumors with mismatch-repair deficiency and head and neck squamous cell carcinoma. Overall, 151 atypical responses were observed in 2400 patients (6%) evaluated by unconventional response criteria.The results of our systematic review highlight the clinical relevance of unconventional responses to anti-PD-1 therapy and support further investigation into the development of tools that may assist evaluation of the antitumor activity of immunotherapy.



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Rehabilitation interventions for upper limb function in the first four weeks following stroke: a systematic review and meta-analysis of the evidence

Publication date: Available online 20 July 2017
Source:Archives of Physical Medicine and Rehabilitation
Author(s): Kimberley A. Wattchow, Michelle N. McDonnell, Susan L. Hillier
ObjectiveTo investigate the therapeutic interventions reported in the research literature, and synthesize their effectiveness in improving upper limb (UL) function in the first four weeks post-stroke.Data sourcesElectronic databases, trial registries and hand searching was conducted, from inception until June 2016.Study selectionRandomised controlled trials (RCTs), controlled trials and interventional studies with pre/post-test design were included for adults within four weeks of any type of stroke with UL impairment. Participants all received an intervention of any physiotherapeutic or occupational therapeutic technique designed to address impairment or activity of the affected UL, which could be compared to usual care, sham or another technique.Data extractionTwo reviewers independently assessed eligibility of full texts, and methodologic quality of included studies using the Cochrane 'Risk of bias tool'.Data synthesis104 trials (83 RCTs, 21 non-randomised studies) were included (n=5,225 participants). Meta-analyses of RCTs only (20 comparisons), and narrative syntheses were completed. Key findings included significant positive effects for modified constraint-induced movement therapy (mCIMT) (standardised mean difference, SMD 1.09, 95% confidence intervals, CI 0.21, 1.97) and task-specific training (SMD 0.37, 95% CI 0.05 to 0.68). Evidence was found to support supplementary use of biofeedback and electrical stimulation. Use of Bobath therapy was not supported.ConclusionsUse of mCIMT and task-specific training was supported, as was supplementary use of biofeedback and electrical simulation, within the acute phase post-stroke. Further high quality studies into the initial four weeks post-stroke are needed to determine therapies for targeted functional UL outcomes.



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Health conditions: impact on function, health-related quality of life, and life satisfaction following traumatic spinal cord injury. A prospective observational registry cohort study

Publication date: Available online 19 July 2017
Source:Archives of Physical Medicine and Rehabilitation
Author(s): Carly S. Rivers, Nader Fallah, Vanessa K. Noonan, David GT. Whitehurst, Carolyn Schwartz, Joel Finkelstein, B. Catharine Craven, Karen Ethans, Colleen O'Connell, Catherine Truchon, Chester Ho, A. Gary Linassi, Christine Short, Eve Tsai, Brian Drew, Henry Ahn, Marcel F. Dvorak, Jérôme Paquet, Michael G. Fehlings, Luc Noreau
ObjectiveTo analyze relationships among injury, demographic and environmental factors on function, HRQoL and life satisfaction in individuals with traumatic SCI.Designprospective observational registry cohort studySetting31 acute and rehabilitation SCI centresParticipantsParticipants (N=340) from the Rick Hansen Spinal Cord Injury Registry (RHSCIR) who were prospectively recruited from 2004-2014 were included. The model cohort included 340 participants: 79.1% were male; mean age was 41.6 (±17.3SD); and 34.7% were motor/sensory complete (AIS A).InterventionsnoneMain Outcome MeasuresPath analysis was used to determine relationships among SCI severity [ASIA Impairment Score (AIS) and anatomical level (cervical/thoracolumbar)], age at injury, education, number of HCs, functional independence [Functional Independence Measure (FIM) motor score], HRQoL [Short Form-36V2 physical (PCS) and mental (MCS) component scores], and life satisfaction [Life Satisfaction-11 (LiSat-11)]. The analysis was conducted using Mplus version 7.1; model fit was assessed using recommended published indices.ResultsGood model fit was supported by all indices, indicating the model results closely matched the RHSCIR data. Higher age, higher severity injuries, cervical injuries, and more HCs negatively affected FIM motor, while employment had a positive effect. Higher age, less education, more severe injuries (AIS A-C), and more HCs negatively correlated with PCS (worse physical health). More HCs were negatively correlated with a lower MCS (worse mental health), however were positively associated with reduced function. Being married and higher function positively affected Lisat-11, but more HCs had a negative effect.ConclusionsComplex interactions and enduring impacts of HCs following SCI have a negative impact on function, HRQoL and life satisfaction. Modeling relationships among these types of concepts will inform clinicians how to positively impact outcomes following SCI; for example, development of screening tools and protocols for managing individuals with traumatic SCI who have multiple HCs.



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Longitudinal Examination of Resilience after Traumatic Brain Injury: A Traumatic Brain Injury Model Systems Study

Publication date: Available online 20 July 2017
Source:Archives of Physical Medicine and Rehabilitation
Author(s): Jennifer H. Marwitz, Adam P. Sima, Jeffrey S. Kreutzer, Laura E. Dreer, Thomas F. Bergquist, Ross Zafonte, Douglas Johnson-Greene, Elizabeth R. Felix
ObjectiveTo evaluate the trajectory of resilience during the first year following a moderate-severe TBI, factors associated with resilience at 3, 6 and 12-months post-injury, and changing relationships over time between resilience and other factors.DesignLongitudinal analysis of an observational cohort.SettingFive inpatient rehabilitation centers.ParticipantsPatients with TBI (N = 195) enrolled in the resilience module of the TBI Model Systems study with data collected at 3, 6, and 12-month follow-up.InterventionsNot applicable.Main Outcome MeasureConnor-Davidson Resilience Scale.ResultsInitially, resilience levels appeared to be stable during the first year post-injury. Individual growth curve models were used to examine resilience over time in relation to demographic, psychosocial, and injury characteristics. After adjusting for these characteristics, resilience actually declined over time. Higher levels of resilience were related to non-minority status, absence of pre-injury substance abuse, lower anxiety and disability level, and greater life satisfaction.ConclusionsResilience is a construct that is relevant to understanding brain injury outcomes and has potential value in planning clinical interventions.



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Novel method to measure active myofascial trigger point stiffness using ultrasound imaging

Publication date: Available online 30 June 2017
Source:Journal of Bodywork and Movement Therapies
Author(s): Mehdi Jafari, Farid Bahrpeyma, Manijhe Mokhtari-Dizaji, Azadeh Nasiri
IntroductionMyofascial trigger points (MTrPs) are one of the most common and important causes of musculoskeletal pain. Ultrasound is a useful modality in examining musculoskeletal disorders. By applying compressive stress and observing changes in ultrasound images, the elastic modulus (Young's modulus) can be calculated. Our objective was to develop a novel method to distinguish MTrPs from normal tissues.MethodsA total of 29 subjects with MTrP in the sternocleidomastoid muscle were assessed. A force gauge was attached to a transducer to obtain stress levels. To obtain strain, images were recorded in both with stress and without stress states. By dividing the stress level by the measured strain, the elastic modulus was determined.ResultsElastic modulus in MTrPs and the normal part of the muscle were measured to be 13379.57 ± 1069.75Pa and 7078.24 ± 482.92Pa, respectively (P = 0.001). This indicated that MTrPs were stiffer than normal parts of the muscle.ConclusionThis study presents a new method for the quantitative measurement of the elastic modulus of MTrP, thereby distinguishing MTrPs from normal adjacent muscular tissue, with more simplicity and lower cost, compared to other ultrasound methods.



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What is the effect and mechanism of kinesiology tape on muscle activity?

Publication date: Available online 27 June 2017
Source:Journal of Bodywork and Movement Therapies
Author(s): Rasool Bagheri, Mohammad Reza Pourahmadi, Ali Reza Sarmadi, Ismail Ebrahimi Takamjani, Giti Torkaman, Sayyed Hamed Fazeli
ObjectiveThis study aimed to evaluate the effects of kinesiology tape, anesthesia, and kinesiology tape along with anesthesia, on motor neuron excitability.ParticipantsParticipants included 20 healthy men aged 20–35 years, who were examined over 5 sessions.InterventionThe five experimental sessions included: control without applying the kinesiology tape or Eutectic Mixture of Local Anesthetics (EMLA); treatment only with EMLA; only kinesiology tape application; only sham tape application; and treatment with kinesiology tape and EMLA.Main outcome measuresThe H-reflex recruitment curve of the soleus and lateral gastrocnemius was recorded by a blinded assessor in the 5 separate sessions randomly assigned with 48 h washout periods. The major H-reflex parameters include: the Hmax/Mmax ratio, the H-reflex threshold stimulation intensity (Hth), the intensity of maximum H-reflex (IntensityHmax), the H-reflex ascending slope (Hslp), and the H-reflex ascending slope fixed into the first three points (first Hslp).ResultsThe H-reflex parameters (H slope, first Hslp, Hth, and IntensityHmax) were facilitated by application of the kinesiology tape with and without EMLA; however, EMLA inhibited the H-reflex parameters (Hmax/Mmax ratio, Hslp, first Hslp, and Hth) in both the soleus and lateral gastrocnemius. The sham tape did not alter the H-reflex recruitment curve parameters. The statistical model revealed a significant difference between the kinesiology tape and the sham tape and control sessions, between kinesiology tape–EMLA and EMLA, and between kinesiology tape–EMLA and control session.ConclusionsResults suggest that the kinesiology tape facilitates the muscle activity and the underlying mechanism on the gastrosoleus motor neuron pool involves the cutaneous receptors.



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The movement movement

Publication date: Available online 24 June 2017
Source:Journal of Bodywork and Movement Therapies
Author(s): Warrick McNeill




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Adolescent idiopathic scoliosis: The Tethered Spine III: Is fascial spiral the key?

Publication date: Available online 24 June 2017
Source:Journal of Bodywork and Movement Therapies
Author(s): Lucy Whyte Ferguson
This article reports on an observational and treatment case series involving 22 adolescents and preadolescents treated over a 15 year period who had or appeared to be developing idiopathic scoliosis (IS). Common patterns of muscle and fascial asymmetry were observed and treated. Most of these individuals had spinal area pain and the trigger points (TrPs) apparently responsible for this pain were located in muscles at some distance from the spine, yet referred pain to locations throughout the thoracolumbar spine. Asymmetries in tension in these muscles appear to tether the spine in such a way as to contribute to scoliotic curvatures. The most common pattern of asymmetrical muscle tension found in these individuals follows a particular spiral fascial plane and as the fascia in this spiral plane tightened, the scoliotic curvature appeared to increase. Evaluation also showed that 21 of 22 of these individuals have major ligamentous laxity and this may also have contributed to the development of scoliotic curvatures. Furthermore, each of these individuals has marked overpronation of the ankles, with arches that totally collapse when weight-bearing. Based on observation during the care of these 22 subjects, it appears that asymmetry in the degree of pronation may, in some individuals, be a contributing factor in the development of the spiral body organization involved in the development of scoliosis. Common patterns of joint dysfunction were also observed in many of these individuals. There were also some findings of significant muscle weaknesses that appeared to contribute to the development of the spiraling body organization. Treatment of the tethering of the spine from myofascial asymmetries and related joint dysfunction not only resulted in elimination of pain in almost all cases but also, in many cases resulted in significant reduction of scoliotic curvatures including reductions of rib humps. In other cases, it resulted in stabilization of scoliotic curvatures, and in some cases it may have slowed the rate of progression of curvatures. Stretching and strengthening exercises appeared to contribute to the stability of treatment gains. A segment of the treated population had atypical scoliotic curvatures that did not follow the typical spiral pattern. Even in these individuals, there were significant patterns of fascial restriction and joint dysfunction accompanying the curvatures. Prior traumatic injury was a common factor in almost all of these atypical cases. Treatment of myofascial TrPs and asymmetrical fascial tension and accompanying joint dysfunction is proposed as a useful approach to treating pain in adolescents who have or who appear to be developing scoliosis and to treating and controlling and/or decreasing many scoliotic curvatures. This case series is presented as a way to illuminate possible factors in the development of scoliosis and promising treatment strategies to address these factors and to eliminate pain and stabilize or decrease curvatures so that further research can more systematically evaluate the role of these factors. An addendum of two more cases is also included, because of the information that these cases add to the discussion and to treatment approaches. Including the addendum cases, 8 of the subjects had scoliosis as documented by x-rays. The other 16 appeared to be developing scoliosis according to criteria stated below.



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Adapting to floor sitting and kneeling

Publication date: Available online 23 June 2017
Source:Journal of Bodywork and Movement Therapies
Author(s): Warrick McNeill
This short practical article suggests that for those unused to ground living, increasing their time spent in sitting or kneeling postures may improve their adaptation of their passive and active joint structures by using low stools, mats, blocks, yoga bricks, bolsters/cushions, and rolled towels. A very slow approach to adaptation and a deliberate reduction of extremes of available lower limb joint range, in the beginning of the process, is likely to reduce possible tissue overload injury risk.



http://ift.tt/2tCEtBl

Orofacial pain of cervical origin: A case report

Publication date: Available online 4 July 2017
Source:Journal of Bodywork and Movement Therapies
Author(s): G. Shankar Ganesh, Mamata Manjari Sahu, Pramod Tigga
BackgroundThe etiopathogenesis of orofacial pain remains complex and a number of pain referral patterns for this region have been reported in the literature. The purpose of this report is to describe the assessment and successful clinical management of orofacial pain possibly attributable to cervical origin.Case descriptionA 55-year-old male teacher with a 3-year history of pain in the right lower jaw, radiating to the ear, consulted our institute for assessment and management. The patient was unsuccessfully treated for dental pain and trigeminal neuralgia. The patient's functioning was grossly limited and the patient was unable to sleep because of severe pain. Current and previous medical and physical examinations revealed no infection, malignancies, or sinusitis. Palpation revealed no temporomandibular disorder, tenderness or myofascial trigger points. Examination of the cervical range of motion showed a reduction in rotation to the right side. The patient was treated for upper cervical joint dysfunction involving mobilization of the first three cervical vertebrae and motor control exercises. The patient had an almost complete resolution of symptoms and reported significant improvement in the Patient Specific Functional Scale (PSFS) and the Global Rating of Change (GRC) scale.ConclusionThis case study demonstrates the importance of considering, assessing and treating the cervical spine as a possible source of orofacial pain, and the positive role of cervical mobilization on these disorders.



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Strength and mobilization training within the first week following total hip arthroplasty

Publication date: Available online 23 June 2017
Source:Journal of Bodywork and Movement Therapies
Author(s): Clarissa Matheis, Thomas Stöggl
Study designRandomized controlled trial.ObjectivesTo improve hip function by an additional targeted mobilization and strength training of the hip muscles within the first postoperative week following a total hip arthroplasty (THA) in contrast to standard physiotherapy.BackgroundThe aim of early postoperative physiotherapy is to improve the functioning of the artificial hip joint as well as the restoration of mobility and independence of the patient. Minimally invasive surgical techniques allow early mobilization with immediate full weight-bearing.Methods39 patients were divided into an intervention (IG) and control group (CG). After implantation of THA the CG completed standard physiotherapy, while the IG had an intensified active treatment with additional mobilization and strength training. Passive range of motion (flexion, extension, abduction), thigh circumference, holding force of the gluteal muscles, one-leg stance, covered distance walked in 6-min and subjective parameters were tested one day before and six days after surgery.ResultsImprovements in IG compared to CG were recorded in range of motion (flexion p < 0.01, extension p < 0.001, abduction p < 0.01) and gait performance (p < 0.001). No differences between groups were detected as regard thigh circumference, holding force of the gluteal muscles, one-leg stance and subjective parameters. Deterioration in IG compared to CG did not occur.ConclusionAn additional, targeted mobilization and strength training of the hip muscles with full weight-bearing, which begins at the 3rd day after implantation of a THA is tolerated well and improves within one week hip range of motion and gait performance compared to standard physiotherapy.



http://ift.tt/2tHThyJ

Abdominal and pelvic floor electromyographic analysis during abdominal hypopressive gymnastics

Publication date: Available online 21 June 2017
Source:Journal of Bodywork and Movement Therapies
Author(s): Lucas Ithamar, Alberto Galvão de Moura Filho, Marco Aurélio Benedetti Rodrigues, Kelly Cristina Duque Cortez, Vinícius Gomes Machado, Claudia Regina Oliveira de Paiva Lima, Eduarda Moretti, Andrea Lemos
IntroductionAbdominal hypopressive gymnastics appeared as an alternative to traditional abdominal exercises to promote abdominal muscles strength without overloading the pelvic floor muscles (PFM). To determine the activation level of abdominal muscles and PFM and the posture influence in the level of activation in these muscles during abdominal hypopressive gymnastics, we used surface electromyography in young and healthy multipara women.MethodsThis is an observational study with eutrophic nulliparous women aged between 18 and 35 years, with abdominal skinfold less than or equal to 3 cm and active or irregularly active physical activity. Surface electromyography was used for rectus abdominis, external oblique, transversus abdominal/internal oblique (TrA/IO) and PFM assessment in the supine, quadruped and orthostatic (upright standing) positions during abdominal hypopressive gymnastics using normalized electromyographic (%EMG) data. We also analyzed the difference in activation between each muscle and between muscles and positions.ResultsThirty women were evaluated and the mean age was 25.77 years (SD 3.29). The group formed by the TrA/IO muscles and the PFM showed higher %EMG in all the positions assessed, followed by the external oblique and rectus abdominis muscles. A comparison of %EMG of each muscle between the different positions showed differences only in rectus abdominis between the supine and quadruped (p = 0.001) and supine and orthostatic positions (p = 0.004), and in TrA/IO between the supine and orthostatic (p = 0.023) and orthostatic and quadruped positions (p = 0.019).ConclusionsThe results suggest that abdominal hypopressive gymnastics can activate the abdominal muscles and PFM and the position do not have influence on electromyographic activation level of the PFM and external oblique.



http://ift.tt/2swt1ZJ

Quantitative tissue parameters of Achilles tendon and plantar fascia in healthy subjects using a handheld myotonometer

Publication date: Available online 21 June 2017
Source:Journal of Bodywork and Movement Therapies
Author(s): Sarah Orner, Wolfgang Kratzer, Julian Schmidberger, Beate Grüner
ObjectiveThe aim of the study was to examine the quantitative tissue properties of the Achilles tendon and plantar fascia using a handheld, non-invasive MyotonPRO device, in order to generate normal values and examine the biomechanical relationship of both structures.DesignProspective study of a large, healthy sample population.ParticipantsThe study sample included 207 healthy subjects (87 males and 120 females) for the Achilles tendon and 176 healthy subjects (73 males and 103 females) for the plantar fascia. For the correlations of the tissue parameters of the Achilles tendon and plantar fascia an intersection of both groups was formed which included 150 healthy subjects (65 males and 85 females).InterventionsAll participants were measured in a prone position. Consecutive measurements of the Achilles tendon and plantar fascia were performed by MyotonPRO device at defined sites.ResultsFor the left and right Achilles tendons and plantar fasciae all five MyotonPRO parameters (Frequency [Hz], Decrement, Stiffness [N/m], Creep and Relaxation Time [ms]) were calculated of healthy males and females. The correlation of the tissue parameters of the Achilles tendon and plantar fascia showed a significant positive correlation of all parameters on the left as well as on the right side.ConclusionsThe MyotonPRO is a feasible device for easy measurement of passive tissue properties of the Achilles tendon and plantar fascia in a clinical setting. The generated normal values of the Achilles tendon and plantar fascia are important for detecting abnormalities in patients with Achilles tendinopathy or plantar fasciitis in the future. Biomechanically, both structures are positively correlated. This may provide new aspects in the diagnostics and therapy of plantar fasciitis and Achilles tendinopathy.



http://ift.tt/2s10sjz

Quadriceps strengthening exercises may not change pain and function in knee osteoarthritis

Publication date: Available online 21 June 2017
Source:Journal of Bodywork and Movement Therapies
Author(s): Hamid Reza Bokaeian, Amir Hoshang Bakhtiary, Majid Mirmohammadkhani, Jamileh Moghimi
It is believed that Quadriceps strength training may reduce pain and improve functional activity in patients with knee osteoarthritis (OA). This improvement is generally attributed to an increase in quadriceps strength. This study investigated whether quadriceps muscle strength increases with decreasing pain, improving functional activity in knee OA. Twenty-four patients with knee OA participated in an 8-week treatment protocol including traditional physical therapy and strength training 3 sessions per week. Measurements were conducted before and after the intervention and included the peak torque of quadriceps muscle, pain by visual analogue scale (VAS), short Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) and functional activity by the 2 minute walking test (2MWT) and time up & go test (TUGT). After the intervention, analysis of data illustrated that changes in quadriceps muscle strength correlated with changes in VAS (r2 = 0.310, p = 0.005), WOMAC (r2 < 0.278, p < 0.008) and 2MWT (r2 < 0.275, p < 0.009) significantly, although the correlation slope was negligible. No correlation was found between muscle strength and TUGT. However, the strength training significantly improved quadriceps muscle strength (p = 0.013), pain and functional activity (p = 0.000). This study showed that reduction in pain and improvement in functional activity occurs independently from an increase in quadriceps muscle strength in knee OA. It seems that increased quadriceps muscle strength may not be a cause of improvement in pain and functional activity in knee OA.



http://ift.tt/2stqK1e

Effects of 16-weeks of Pilates on functional autonomy and life satisfaction among elderly women

Publication date: Available online 21 June 2017
Source:Journal of Bodywork and Movement Therapies
Author(s): Vanessa Sanders Curi, Helder Miguel Fernandes, José Vilaça, Aline Nogueira Haas
The aim of this study was to determine the effects of Pilates-based mat exercises on functional autonomy and life satisfaction among elderly women. A randomized controlled trial was conducted in Caxias do Sul, Brazil in 2015, in which 61 healthy older women were randomized into two groups (experimental group, EG = 31 participants with 64.25 mean years of age, SD = 0.14, and a control group, CG = 30 participants, 63.75 mean years of age; SD = 0.08). The EG participants performed the mat Pilates twice per week, 60-minutes per session. The same measurements were performed at baseline and after 16 weeks. No significant differences were found between groups at baseline (p > 0.05). In the EG, the following factors were increased after the 16-weeks training period: flexibility of lower limbs, flexibility of upper limbs, strength in lower and upper limbs, aerobic endurance and dynamic balance. This study revealed significant improvements on the life satisfaction scale's score for the EG when compared to the CG, after 16 weeks of intervention. No differences were found between baseline and after 16 weeks on the functional autonomy and life satisfaction scores for the CG. According to the results of this study, it was found that functional autonomy and life satisfaction for elderly women was improved after exposure to 16 weeks of the PM, suggesting this practice helps with healthy aging.



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Osteopathic manipulative treatment in chronic coccydynia: A case series

Publication date: Available online 19 June 2017
Source:Journal of Bodywork and Movement Therapies
Author(s): D. Origo, A.G. Tarantino, A. Nonis, L. Vismara
BackgroundCoccydynia is a disorder associated with pain/discomfort at the base of the spine. The role of osteopathic manipulative treatment (OMT) in chronic coccydynia as well as for low back pain (LBP) and radicular pain (RP) associated with coccydynia, has not previously been investigated. This study seeks to analyse the effects of OMT on chronic coccydynia compared to physical therapy and pharmacological treatment (PTPT). The secondary objective is to analyse the effect of OMT on LBP and RP associated with coccydynia.MethodsClinical records of 50 patients were examined. These patients (aged 39.94 ± 15.34 years, BMI 21.22 ± 3.15) who complained of chronic coccydynia were assessed 3 times: before any treatment (t0), after PTPT (t1) and after OMT (t2). Patients were treated with PTPT during the first 3 months and then referred by physicians to osteopaths to receive 3 sessions of OMT over a period of 5 weeks. The outcome measurements were made by a visual analogue scale (VAS 0–10 cm) and the Oswestry Low Back Pain Disability Questionnaire.ResultsBefore starting OMT treatment, patients showed a stable condition of coccydynia (mean VAS values from 7.1 to 6.5 p = 0.065) and a slight but significant reduction in disability (mean OD values from 17.7 to 14.5 p = 0.017) after PTPT. After the 3 sessions of OMT, all subjects gained a successful reduction in pain (mean VAS values from 6.5 to 1.2, p ≤ 0.001) and demonstrated a higher significant reduction in disability (mean Oswestry scale values from 14.5 to 2.5, p < 0.001).ConclusionsThis case series shows that OMT elicits a positive benefit for pain relief and reduction in disability in patients complaining of coccydynia (with or without LBP and RP associated with coccydynia). Therefore, OMT could be considered as a valid therapeutic approach for treating chronic coccydynia. Nevertheless, further research is required to test the hypothesis and to better determine the benefits of OMT.



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Effect of kinesio taping on lower limb joint powers in individuals with genu varum

Publication date: Available online 17 June 2017
Source:Journal of Bodywork and Movement Therapies
Author(s): AmirAli Jafarnezhadgero, Morteza Madadi Shad, Mahdi Majlesi, Matteo Zago
ObjectiveTherapeutic lateral knee joint muscle taping potentially offers a low-risk, economical and effective alternative for the clinical treatment of light to moderate knee overload, due to misalignment in patients with genu varum. In this study, we aimed at investigating the immediate effect of lateral knee joint muscular kinesio taping on lower limb joint powers, during the stance phase of walking, in individuals with genu varum.MethodsFifteen male subjects with genu varum misalignment (age: 24.2±3.7 years) participated in the study. Subjects performed three walking trials without, and three with, biceps femoris and vastus lateralis kinesio taping. The three-dimensional position coordinate data of reflective markers were collected at 100 Hz using a six-cameras Vicon system (Motion Analysis Corp., UK). Additionally, two Kistler force plates (Kistler AG, Winterthur, Switzerland) were used to record the Ground Reaction Forces (GRF) components at 1000 Hz during stance phase of walking. A three-way ANOVA with post-hoc testing (using paired samples Student's t-test with Bonferroni correction) was performed to compare the power values of lower limb joints before and after the use of KT.ResultsWith kinesio taping, we observed that the average negative power increased at the ankle level in dominant limb, (P<0.05, 10-20% of gait cycle, GC), and at the knee level in both limbs (10-20% and 60-80% GC). Further, average negative power of the non-dominant knee joint (80-100% GC) and positive power of the non-dominant hip joint (60-80% GC) significantly reduced (P<0.05) in kinesio taping condition.ConclusionThe biomechanical analysis of joint power during walking using kinesio taping provided essential information about the possible mechanisms involved in gait analysis with this intervention in adults with genu varus.



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Identifying novel members of the Wntless interactome through genetic and candidate gene approaches

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Publication date: Available online 20 July 2017
Source:Brain Research Bulletin
Author(s): Jessica Petko, Trevor Tranchina, Goral Patel, Robert Levenson, Stephanie Justice-Bitner
Wnt signaling is an important pathway that regulates several aspects of embryogenesis, stem cell maintenance, and neural connectivity. We have recently determined that opioids decrease Wnt secretion, presumably by inhibiting the recycling of the Wnt trafficking protein Wntless (Wls). This effect appears to be mediated by protein-protein interaction between Wls and the mu-opioid receptor (MOR), the primary cellular target of opioid drugs. The goal of this study was to identify novel protein interactors of Wls that are expressed in the brain and may also play a role in reward or addiction. Using genetic and candidate gene approaches, we show that among a variety of protein, Wls interacts with the dopamine transporter (target of cocaine), cannabinoid receptors (target of THC), Adenosine A2A receptor (target of caffeine), and SGIP1 (endocytic regulator of cannabinoid receptors). Our study shows that aside from opioid receptors, Wntless interacts with additional proteins involved in reward and/or addiction. Future studies will determine whether Wntless and WNT signaling play a more universal role in these processes.



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The olfactory bulbectomized rat model is not an appropriate model for studying depression based on morphological/stereological studies of the hippocampus

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Publication date: Available online 20 July 2017
Source:Brain Research Bulletin
Author(s): Canan Yurttas, Christoph Schmitz, Mehmet Turgut, Tatyana Strekalova, Harry W.M. Steinbusch
Bilateral olfactory bulbectomy (OBX) has been used as an animal model for major depression that results in behavioral, neurochemical, and neuroendocrinological changes were reversed by chronic treatment with antidepressants, including fluoxetine. However, both etiological and construct validities are lacking in OBX for rats. In the present study, we investigated the morphological changes in the hippocampi of rats undergoing OBX that were treated with fluoxetine (10mg/kg, p.o. once daily for 4 and 12 weeks) using stereological techniques. Our results revealed that OBX caused a reduction in the volumes of the CA1/2, CA3, and dentate gyrus regions 4 weeks after OBX without fluoxetine treatment. With fluoxetine treatment, these reductions were achieved 12 weeks after OBX and the volumes were comparable to normal control rats. Nevertheless, fluoxetine treatment did not reverse neuron loss in all hippocampal regions 12 weeks after OBX. Therefore, we suggest that the OBX rat model should not be used to detect the antidepressant activity of various pharmacological agents such as fluoxetine.



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Molecular basis for the functions of a bacterial MutS2 in DNA repair and recombination

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Publication date: Available online 19 July 2017
Source:DNA Repair
Author(s): Ge Wang, Robert J. Maier
Bacterial MutS2 proteins, consisting of functional domains for ATPase, DNA-binding, and nuclease activities, play roles in DNA recombination and repair. Here we observe a mechanism for generating MutS2 expression diversity in the human pathogen Helicobacter pylori, and identify a unique MutS2 domain responsible for specific DNA-binding. H. pylori strains differ in mutS2 expression due to variations in the DNA upstream sequence containing short sequence repeats. Based on Western blots, mutS2 in some strains appears to be co-translated with the upstream gene, but in other strains (e.g. UA948) such translational coupling does not occur. Accordingly, strain UA948 had phenotypes similar to its ΔmutS2 derivative, whereas expression of MutS2 at a separate locus in UA948 (the genetically complemented strain) displayed a lower mutation rate and lower transformation frequency than did ΔmutS2. A series of truncated HpMutS2 proteins were purified and tested for their specific abilities to bind 8-oxoG-containing DNA (GO:C) and Holiday Junction structures (HJ). The specific DNA binding domain was localized to an area adjacent to the Smr nuclease domain, and it encompasses 30-amino-acid-residues containing a "KPPKNKFKPPK" motif. Gel shift assays and competition assays supported that a truncated version of HpMutS2-C12 (∼12kDa protein containing the specific DNA-binding domain) has much greater capacity to bind to HJ or GO:C DNA than to normal double stranded DNA. By studying the in vivo roles of the separate domains of HpMutS2, we observed that the truncated versions were unable to complement the ΔmutS2 strain, suggesting the requirement for coordinated function of all the domains in vivo.



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Monitoring and modeling 4-chlorophenol biodegradation kinetics by phenol-acclimated activated sludge by using open respirometry

Abstract

The aim of this study was to analyze the mechanisms, stoichiometry, and stability of 4-chlorophenol (4CP) biodegradation kinetics by phenol-acclimated activated sludge using open respirometry. While the removal of 4CP was higher than 98%, the removal of chemical oxygen demand (COD) ranged between 69 and 79% due to the accumulation of an intermediate metabolite. The value obtained from respirometric profiles for the stoichiometric ratio of O2 to 4CP (YO2/4CP) was 1.95 ± 0.04 mol of oxygen consumed per mol of 4CP removed. This YO2/4CP value reflected the action of the oxygenases responsible for the first steps of the aerobic oxidation of 4CP. The 4CP degradation activity decreased noticeably when successive pulses of 4CP were added to the respirometer. A mathematical model was developed to represent the aerobic biodegradation of 4CP. The fitted model adequately predicted the oxygen consumption rate, total phenols, and soluble COD concentrations as a function of time. The results presented could help to predict the dynamic of biodegradation of chlorophenols in a biological wastewater treatment system.



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Respiratory arrest at the onset of idiopathic childhood occipital epilepsy of Gastaut

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Publication date: Available online 19 July 2017
Source:Brain and Development
Author(s): Keiko Funata, Tatsuhiko Shike, Toshiki Takenouchi, Yukio Yamashita, Takao Takahashi
Occipital lobe epilepsy of childhood includes two entities: Panayiotopoulos syndrome in pre-school children, and idiopathic childhood occipital epilepsy of Gastaut (ICOEG) in school-age children. The typical initial manifestation of the former is vomiting, and that of the latter is visual hallucinations. Ictal cardiopulmonary arrest at initial presentation has been reported for Panayiotopoulos syndrome, but not for ICOEG. We document a 7-year-old previously healthy girl who experienced an acute elemental visual hallucination of seeing insects, followed by a new-onset generalized seizure. Upon arrival at the local hospital, she was unconscious and soon thereafter, developed respiratory arrest. She was resuscitated and initiated on mechanical ventilation. An electroencephalogram taken three days after seizure cessation showed frequent occipital spikes, consistent with the diagnosis of ICOEG. The sequence of acute elementary visual hallucination followed by a motor seizure, and then witnessed respiratory arrest illustrated occurrence of life-threatening autonomic involvement at initial onset in ICOEG. We speculate that the epileptic propagation from the occipital lobes eventually compromised the respiratory center in the brainstem. The possibility of occipital lobe epilepsy should be considered in school-age children presenting with acute visual hallucination followed by respiratory arrest. Such a presentation should prompt an urgent electroencephalogram and initiation of antiepileptic treatment if indicated.



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Cerebellar fits in the 2000s

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Publication date: Available online 19 July 2017
Source:Brain and Development
Author(s): Domenico Serino, Davide Caputo, Lucia Fusco
Acute compression on the brainstem or acute increase in intracranial pressure may induce non-epileptic events varying from tonic seizures to axial rigidity with motor automatism, sometimes clearly characterized by decerebrate or decorticate paroxysmal posturing. The EEG correlate is characterized by diffuse asynchronous slow waves of variable amplitude. The mechanism behind such events, known as "cerebellar seizures or fits", is linked to cerebellar herniation and brainstem compression and is not of cortical origin. Misrecognition of such entity may entail an incongruous therapeutic intervention in a life-threatening situation. We describe two emblematic paediatric cases of cerebellar fits caused by diffuse oedema and brainstem compression: a 10-year-old girl with acute disseminated encephalomyelitis (ADEM) and a 2-year-old girl with severe respiratory distress symptomatic of Fallot tetralogy. We also describe the EEG correlate recorded during the events.



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Versatile distally based neurocutaneous saphenous flap in the era of microsurgery

Abstract

Background

The reconstruction of soft tissue defects in the distal third of the leg and ankle joint remains a challenge for reconstructive surgeons. Presently, the distally based neurocutaneous flaps are the predominant surgical alternatives to free tissue transfer. In this article, we describe our experience with the distally based saphenous neurocutaneous flap for reconstructing soft tissue defects in this region.

Methods

A retrospective review of all patients who underwent reconstruction of the leg distal third and ankle joint at our department from January 1996 to June 2016 was carried out. Only patients who underwent reconstructive surgery for acute traumatic injuries were included. The fasciocutaneous pedicle was 3–4 cm wide, and the anatomical structures consisted of the superficial and deep fascia, saphenous nerve, greater saphenous vein and their accompanying vessels together with an islet of subcutaneous cellular tissue and skin. The size of skin islands ranged from 5–7 to 10–14 cm2.

Results

A total of 170 patients (142 men and 28 women) were included in this study. The mean follow-up period was 3 months. All flaps survived with partial necrosis in three patients. Severe venous congestion occurred only in five patients. However, the flap was eventually salvaged after limb elevation and interrupted bloodletting. All donor sites were closed using split-skin grafting.

Conclusions

The reverse saphenous neurocutaneous flap proved to be a reliable and efficient method for reconstructing soft tissue defects in the distal third of the leg and ankle joint.

Level of Evidence: Level IV, therapeutic study



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Vascular regulation of glioma stem-like cells: a balancing act

Publication date: December 2017
Source:Current Opinion in Neurobiology, Volume 47
Author(s): Lucy J Brooks, Simona Parrinello
Glioblastoma (GBM) are aggressive and therapy-resistant brain tumours driven by glioma stem-like cells (GSCs). GSC behaviour is controlled by the microenvironment, or niche, in which the cells reside. It is well-established that the vasculature is a key component of the GSC niche, which drives maintenance in the tumour bulk and invasion at the margin. Emerging evidence now indicates that the specific properties of the vasculature within these two regions impose different functional states on resident GSCs, generating distinct subpopulations. Here, we review these recent findings, focusing on the mechanisms that underlie GSC/vascular communication. We further discuss how plasticity enables GSCs to respond to vascular changes by interconverting bidirectionally between states, and address the therapeutic implications of this dynamic response.



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Functional density and edge maps: Characterizing functional architecture in individuals and improving cross-subject registration

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Publication date: September 2017
Source:NeuroImage, Volume 158
Author(s): Tong Tong, Iman Aganj, Tian Ge, Jonathan R. Polimeni, Bruce Fischl
Population-level inferences and individual-level analyses are two important aspects in functional magnetic resonance imaging (fMRI) studies. Extracting reliable and informative features from fMRI data that capture biologically meaningful inter-subject variation is critical for aligning and comparing functional networks across subjects, and connecting the properties of functional brain organization with variations in behavior, cognition and genetics. In this study, we derive two new measures, which we term functional density map and edge map, and demonstrate their usefulness in characterizing the function of individual brains. Specifically, using data from the Human Connectome Project (HCP), we show that (1) both functional maps capture intrinsic properties of the functional connectivity pattern in individuals while exhibiting large variation across subjects; (2) functional maps derived from either resting-state or task-evoked fMRI can be used to accurately identify subjects from a population; and (3) cross-subject alignment using these functional maps considerably reduces functional variation and improves functional correspondence across subjects over state-of-the-art multimodal registration algorithms. Our results suggest that the proposed functional density and edge maps are promising features in characterizing the functional architecture in individuals and provide an alternative way to explore the functional variation across subjects.



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Sex-linked association between cortical scene selectivity and navigational ability

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Publication date: September 2017
Source:NeuroImage, Volume 158
Author(s): Xiang-Zhen Kong, Yi Huang, Xin Hao, Siyuan Hu, Jia Liu
Spatial navigation is a crucial ability for living. Previous studies have shown that males are better at navigation than females, but little is known about the neural basis underlying the sex differences. In this study, we investigated whether cortical scene processing in three well-established scene-selective regions was sexually different, by examining sex differences in scene selectivity and its behavioral relevance to navigation. To do this, we used functional magnetic resonance imaging (fMRI) to scan the parahippocampal place area (PPA), retrosplenial complex (RSC), and occipital place area (OPA) in a large cohort of healthy young adults viewing navigationally relevant scenes (N = 202), and correlated their neural selectivity to scenes with their self-reported navigational ability. Behaviorally, we replicated the previous finding that males were better at navigation than females. Neurally, we found that the scene selectivity in the bilateral PPA, not in the RSC or OPA, was significantly higher in males than females. Such differences could not be explained by confounding factors including brain size and fMRI data quality. Importantly, males, not females, with stronger scene selectivity in the left PPA possessed better navigational ability. This brain-behavior association could not be accounted for by non-navigational abilities (i.e., intelligence and mental rotation ability). Overall, our study provides novel empirical evidence demonstrating sex differences in the brain activity, inviting further studies on sex differences in the neural network for spatial navigation.



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Evaluating the replicability, specificity, and generalizability of connectome fingerprints

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Publication date: September 2017
Source:NeuroImage, Volume 158
Author(s): Lea Waller, Henrik Walter, Johann D. Kruschwitz, Lucia Reuter, Sabine Müller, Susanne Erk, Ilya M. Veer
Establishing reliable, robust, and unique brain signatures from neuroimaging data is a prerequisite for precision psychiatry, and therefore a highly sought-after goal in contemporary neuroscience. Recently, the procedure of connectome fingerprinting, using brain functional connectivity profiles as such signatures, was shown to be able to accurately identify individuals from a group of 126 subjects from the Human Connectome Project (HCP). However, the specificity and generalizability of this procedure were not tested. In this replication study, we show both for the original and an extended HCP data set (n = 900 subjects), as well as for an additional data set of more commonly acquired imaging quality (n = 84) that (i) although the high accuracy can be replicated for the larger HCP 900 data set, accuracy is (ii) lower for standard neuroimaging data, and, that (iii) connectome fingerprinting may not be specific enough to distinguish between individuals. In addition, both accuracy and specificity are projected to drop considerably as the size of a data set increases. Although the moderate-to-high accuracies do suggest there is a portion of unique variance, our results suggest that connectomes may actually be quite similar across individuals. This outcome may be relevant to how precision psychiatry could benefit from inferences based on functional connectomes.



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Invariance of surface color representations across illuminant changes in the human cortex

Publication date: September 2017
Source:NeuroImage, Volume 158
Author(s): Michael M. Bannert, Andreas Bartels
A central problem in color vision is that the light reaching the eye from a given surface can vary dramatically depending on the illumination. Despite this, our color percept, the brain's estimate of surface reflectance, remains remarkably stable. This phenomenon is called color constancy. Here we investigated which human brain regions represent surface color in a way that is invariant with respect to illuminant changes. We used physically realistic rendering methods to display natural yet abstract 3D scenes that were displayed under three distinct illuminants. The scenes embedded, in different conditions, surfaces that differed in their surface color (i.e. in their reflectance property). We used multivariate fMRI pattern analysis to probe neural coding of surface reflectance and illuminant, respectively. While all visual regions encoded surface color when viewed under the same illuminant, we found that only in V1 and V4α surface color representations were invariant to illumination changes. Along the visual hierarchy there was a gradient from V1 to V4α to increasingly encode surface color rather than illumination. Finally, effects of a stimulus manipulation on individual behavioral color constancy indices correlated with neural encoding of the illuminant in hV4. This provides neural evidence for the Equivalent Illuminant Model. Our results provide a principled characterization of color constancy mechanisms across the visual hierarchy, and demonstrate complementary contributions in early and late processing stages.



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Quicksilver: Fast predictive image registration – A deep learning approach

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Publication date: September 2017
Source:NeuroImage, Volume 158
Author(s): Xiao Yang, Roland Kwitt, Martin Styner, Marc Niethammer
This paper introduces Quicksilver, a fast deformable image registration method. Quicksilver registration for image-pairs works by patch-wise prediction of a deformation model based directly on image appearance. A deep encoder-decoder network is used as the prediction model. While the prediction strategy is general, we focus on predictions for the Large Deformation Diffeomorphic Metric Mapping (LDDMM) model. Specifically, we predict the momentum-parameterization of LDDMM, which facilitates a patch-wise prediction strategy while maintaining the theoretical properties of LDDMM, such as guaranteed diffeomorphic mappings for sufficiently strong regularization. We also provide a probabilistic version of our prediction network which can be sampled during the testing time to calculate uncertainties in the predicted deformations. Finally, we introduce a new correction network which greatly increases the prediction accuracy of an already existing prediction network. We show experimental results for uni-modal atlas-to-image as well as uni-/multi-modal image-to-image registrations. These experiments demonstrate that our method accurately predicts registrations obtained by numerical optimization, is very fast, achieves state-of-the-art registration results on four standard validation datasets, and can jointly learn an image similarity measure. Quicksilver is freely available as an open-source software.



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Phase properties of transcranial electrical stimulation artifacts in electrophysiological recordings

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Publication date: September 2017
Source:NeuroImage, Volume 158
Author(s): Nima Noury, Markus Siegel
Monitoring brain activity during transcranial electric stimulation (tES) is an attractive approach for causally studying healthy and diseased brain activity. Yet, stimulation artifacts complicate electrophysiological recordings during tES. Design and evaluation of artifact removal methods require a through characterization of artifact features, i.e. characterization of the transfer function that defines the relationship between the tES stimulation current and tES artifacts. Here we characterize the phase relationship between stimulation current and tES artifacts in EEG and MEG. We show that stimulation artifacts are not pure in-phase or anti-phase signals, but that non-linear mechanisms induce steady phase deflections relative to the stimulation current. Furthermore, phase deflections of stimulation artifacts are slightly modulated by each heartbeat and respiration. For commonly used stimulation amplitudes, artifact phase deflections correspond to signals several times bigger than normal brain signal. Moreover, the strength of phase deflections varies with stimulation frequency. These phase effects should be accounted for during artifact removal and when comparing recordings with different stimulation frequencies. We summarize our findings in a mathematical model of tES artifacts and discuss how this model can be used in simulations to design and evaluate artifact rejection techniques. To facilitate this research, all raw data of this study is made freely available.



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Characterization of Candidate probionts isolated from human breast milk.

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Characterization of Candidate probionts isolated from human breast milk.

Cell Mol Biol (Noisy-le-grand). 2017 May 20;63(5):82-88

Authors: Khalkhali S, Mojgani N

Abstract
This study was designed to isolate and identify the potential probionts present in 32 healthy mothers' breast milk. Microbial culture media and 16SrRNA sequencing were used to isolate and identify the bacteria and all isolates were analyzed for their antagonistic potential, resistance to acidic pH, bile salts and survival under simulated gastric and intestinal conditions. The colonization potential was further assessed based on adherence to human enterocyte-like Caco-2 cell lines. The breast milk samples harbored significant numbers of Gram positive and catalase negative (85%) bacteria. Based on 16SrRNA sequencing, these isolates were identified as Lactobacillus casei, L.gasseri, L.fermentum, L.plantarum, Pediococcus acidilactici, and Enterococcus facieum. Among the isolates, P. acidilactici was the most frequent species (71%) present in these samples. Few Gram and catalase positive isolates, Staphylococcus aureus and S.hominiis were also observed. The isolates were viable and unviable in pH 3 and 1.5, respectively, while all isolates survived in 1.0% bile salt. As putative probionts, P.acidilactici 1C showed a significantly higher percentage of adhesion to Caco-2 cells (p< 0.05)than the other two isolates L.plantarum 7A and E.facieum 2C. Bacterial strains isolated from human breast milk were shown to have probiotic properties including anti-infective protection and may be considered as future therapeutics for infants.

PMID: 28719350 [PubMed - in process]



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The Spectrum of Vitamin D Deficiency: Description of a Family

09-2016-0359-endo_10-1055-s-0043-109699-

Exp Clin Endocrinol Diabetes 2017; 125: 478-484
DOI: 10.1055/s-0043-109699

Background Vitamin D deficiency represents a global health problem, affecting children and adolescents worldwide. Objects To confirm that vitamin D deficiency can present as a spectrum of clinical pictures. Methods We diagnosed nutritional rickets in a 10-month-old infant of Senegal origin with several risk factors for vitamin D deficiency. As many of these factors affected also his cohabitant relatives, we evaluate infant's family members (mother and 4 brothers) looking for other vitamin D deficiency-related comorbidities. Results 3 brothers had asymptomatic vitamin D deficiency and 2 of them (9.8 and 13.4 years-old) showed secondary hyperparathyroidism. The fourth brother (11.3 years-old) had nutritional rickets. Their mother was affected by osteomalacia. None of them received vitamin D supplementation. Conclusion Vitamin D deficiency may present as a spectrum of clinical pictures, representing a continuum ranging from asymptomatic/subtle conditions to overt rickets/osteomalacia. Immigrant families are at high risk for vitamin D deficiency at every age. If a case of symptomatic vitamin D deficiency is recognized, then the evaluation of the all family members is recommended, as they can have the same and/or other risk factors for vitamin D deficiency.
[...]

© Georg Thieme Verlag KG Stuttgart · New York

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Circulating Insulin-like Growth Factor-1 and Insulin-like Growth Factor Binding Protein-3 predict Three-months Outcome after Ischemic Stroke

07-2016-0282-endo_10-1055-s-0043-103965-

Exp Clin Endocrinol Diabetes 2017; 125: 485-491
DOI: 10.1055/s-0043-103965

Reports on neuroprotective effects of Insulin-like growth factor-1 (IGF-1) and Insulin-like growth factor binding protein-3 (IGFBP-3) in ischemic brain tissue are inconsistent. The aim of this study was to determine if plasma levels of IGF-1 and IGFBP-3 in acute stroke patients are indicative of 3 months functional outcome. Plasma levels were measured via chemiluminescence immunoassay in heparin blood samples of patients included in the EARLY trial (NCT00562588). Plasma samples were drawn on admission and 8 days post-stroke. Neurological deficits were assessed via modified Rankin Scale (mRS) 3 months post-stroke, resulting in favorable (mRS=0–2) or unfavorable (mRS=3–6) outcome. A multiple binary logistic regression including IGF-1 and IGFBP-3 levels and confounders was conducted. Out of 404 included patients, 89 patients had an unfavorable outcome. Mean mRS on admission as well as 3 months post-stroke was 2 (±1). Low IGF-1 levels (day 8) were independently associated with a decreased risk of an unfavorable outcome (OR 0.61; 95%CI 0.37–0.99; p=0.044). Low IGFBP-3 levels (day 8) were independently associated with an unfavorable outcome (OR 2.75; 95%CI 1.56–4.84; p<0.001). Low IGFBP-3 levels and high IGF-1 levels in the subacute phase are predictive of unfavorable outcome 3 months after stroke.
[...]

© Georg Thieme Verlag KG Stuttgart · New York

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Efficacy and Safety of GLP-1 Receptor Agonists Across the Spectrum of Type 2 Diabetes Mellitus

12-2016-0493-dia_10-1055-s-0043-103969-1

Exp Clin Endocrinol Diabetes 2017; 125: 419-435
DOI: 10.1055/s-0043-103969

For patients with type 2 diabetes mellitus, glucagon-like peptide-1 receptor agonists (GLP-1RAs) generally exert robust glucose-lowering effects that are at least as effective as insulin. As monotherapies, changes from baseline in HbA1c with GLP-1RAs ranged from –1.9 to –0.7% in phase 3 trials. In addition, GLP-1RAs confer a low risk of hypoglycaemia and have a body-weight advantage (changes from baseline ranging from –4.0 to –0.4 kg). There is also evidence of significant reductions in risk for cardiovascular events with some of these agents, with a number of other trials underway. Gastrointestinal adverse events typically increase with GLP-1RAs, although these are generally mild to moderate in intensity and rarely require treatment discontinuation. The GLP-1RAs that are commercially available or in development vary in structure and pharmacokinetics. These differences affect the frequency of administration and can also affect their relative efficacy and safety. This review summarizes the findings of phase 3 glycaemic control trials of available GLP-1RAs and considers them in the context of the distinct clinical needs of individual patients.
[...]

© Georg Thieme Verlag KG Stuttgart · New York

Article in Thieme eJournals:
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The Effect of Resistance Exercise on Sleep: A Systematic Review of Randomized Controlled Trials

Impaired sleep quality and quantity are associated with future morbidity and mortality. Exercise may be an effective non-pharmacological intervention to improve sleep, however, little is known on the effect of resistance exercise. Thus, we performed a systematic review of the literature to determine the acute and chronic effects of resistance exercise on sleep quantity and quality. Thirteen studies were included. Chronic resistance exercise improves all aspects of sleep, with the greatest benefit for sleep quality.

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Parent reported nutritional risk and laboratory indices of cardiometabolic risk and in preschool-aged children

Journal Name: Journal of Pediatric Endocrinology and Metabolism
Issue: Ahead of print


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Episodic memory following deep brain stimulation of the ventral anterior limb of the internal capsule and electroconvulsive therapy

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Publication date: Available online 19 July 2017
Source:Brain Stimulation
Author(s): Isidoor O. Bergfeld, Mariska Mantione, Mechteld L.C. Hoogendoorn, Ferdinand Horst, Peter Notten, P. Richard Schuurman, Damiaan Denys
BackgroundElectroconvulsive Therapy (ECT) and Deep Brain Stimulation (DBS) are effective treatments for patients with treatment-resistant depression (TRD). However, a common side effect of ECT is autobiographical memory loss (e.g., personal experiences), whereas the impact of DBS on autobiographical memories has never been established.ObjectiveComparing autobiographical memories following DBS and ECT.MethodsIn two hospitals in The Netherlands, we interviewed 25 TRD patients treated with DBS of the ventral anterior limb of the internal capsule (vALIC), 14 TRD patients treated with ECT and 22 healthy controls (HC) with the Autobiographical Memory Inventory – Short Form (AMI-SF) in a prospective, longitudinal study between March 2010 and August 2016. Patients treated with DBS were interviewed before surgery, after surgery, and twice during treatment over 122.7 (SD: ±22.2) weeks. Patients treated with ECT were tested before ECT, after six right unilateral (RUL) ECT sessions and twice following ECT over 65.1 (±9.3) weeks. Controls were tested four times over 81.5 (±15.6) weeks.ResultsCompared to HC, the AMI-SF score decreased faster in both TRD groups (P<0.001). More specifically, AMI-SF score decreased in a comparable rate as HC after DBS surgery, but decreased more during treatment. The AMI-SF decrease in the ECT group was larger than both the DBS and HC groups.ConclusionsBoth ECT and vALIC DBS result in a faster autobiographical memory decline compared to HC. DBS might have a negative impact on autobiographical memories, although less so than ECT. Future work should dissect whether DBS or characteristics of TRD cause this decline.



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Modelling the effects of water diversion and combined sewer overflow on urban inland river quality

Abstract

In order to assist and optimize the operation of a clean water diversion project for the medium-sized inland rivers in Chaohu, China, an integrated hydrodynamic and water quality model was used in this study. Sixteen diversion scenarios and five sewage interception scenarios were defined to assess the improvement of water quality parameters including ammonia nitrogen (NH3-N), total phosphorus (TP) and chemical oxygen demand (COD) under different diverted water flows, diverting times, diverting points, diverting routines and sewage interception proportions. An index of pollutant removal rate per unit diverted water flow (PRUWF) was proposed to evaluate the effect of the clean water diversion. Results show that operating conditions played important roles in water quality improvement of medium-sized inland rivers. The optimal clean water diversion was operated under the conditions of a flow rate of 5 m3/s for 48 h with an additional constructed bridge sluice. A global sensitivity analysis using the Latin Hypercube One-Factor-at-a-Time (LH-OAT) method was conducted to distinguish the contributions of various driving forces to inland river water restoration. Results show that sewage interception was more important than diverted water flow and diverting time with respect to water quality improvement, especially for COD.



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The trajectory of bone surface modification studies in paleoanthropology and a new Bayesian solution to the identification controversy

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Publication date: September 2017
Source:Journal of Human Evolution, Volume 110
Author(s): Jacob A. Harris, Curtis W. Marean, Kiona Ogle, Jessica Thompson
A critical issue in human evolution is how to determine when hominins began incorporating significant amounts of meat into their diets. This fueled evolution of a larger brain and other adaptations widely considered unique to modern humans. Determination of the spatiotemporal context of this shift rests on accurate identification of fossil bone surface modifications (BSM), such as stone tool butchery marks. Multidecade-long debates over the agents responsible for individual BSM are indicative of systemic flaws in current approaches to identification. Here we review the current state of BSM studies and introduce a novel probabilistic approach to identifying agents of BSM. We use control assemblages of bones modified by modern agents to train a multivariate Bayesian probability model. The model then identifies BSM agents with associated uncertainties, serving as the basis for a predictive probabilistic algorithm. The multivariate Bayesian approach offers a novel, probabilistic, and analytical method for BSM research that overcomes much of the bias that has typified previous, more qualitative approaches.



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Evaluation of the effects of photobiomodulation on vertebras in two rat models of experimental osteoporosis

Abstract

The aim of this study was to evaluate the effects of photobiomodulation (PBM) on cancellous bone in rat models of ovariectomized induced osteoporosis (OVX-D) and glucocorticoid-induced osteoporosis (GIOP). The experiment comprised of nine groups. A group of healthy rats was used for baseline evaluations. The OVX-D rats were further divided into groups as follows: control rats with osteoporosis, OVX-D rats that received alendronate (1 mg/kg 60 days), OVX-D rats treated with pulsed wave laser (890 nm, 80 Hz, 900 s, 0.0061 W/cm2, 5.5 J/cm2, three times a week, 60 days), and OVX-D rats treated with alendronate + pulsed laser. Dexamethasone was administered to the remaining rats that were split into four groups: control, alendronate-treated rats, laser-treated rats, and GIOP rats treated with alendronate + laser. T12, L1, L2, and L3 vertebrae were subjected to laser. Results of the current study demonstrated that OVX-D and GIOP significantly decreased some stereological parameters, and type 1 collagen gene expression compared to the healthy group. There was a significant increase in osteoclast number in both OVX-D and glucocorticoid administration compared to the healthy group. However, the detrimental effect of the OVX-D procedure on bone was more serious than glucocorticoid administration. Results showed that laser alone had a detrimental effect on trabecular bone volume, and cortical bone volume in groups GIOP and OVX-D compared to those in the healthy group. Alendronate significantly improved total vertebral bone volume, trabecular bone volume, and cortical bone volume, in GIOP and OVX-D groups compared to the laser-treated groups. Furthermore, the alendronate + laser in OVX-D rats and GIOP rats produced significantly increased osteoblast number and type 1 collagen gene expression and caused a significant decrease in osteoclast number compared to the controls.



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Sparse MRI reconstruction using multi-contrast image guided graph representation

Publication date: Available online 19 July 2017
Source:Magnetic Resonance Imaging
Author(s): Zongying Lai, Xiaobo Qu, Hengfa Lu, Xi Peng, Di Guo, Yu Yang, Gang Guo, Zhong Chen
Accelerating the imaging speed without sacrificing image structures plays an important role in magnetic resonance imaging. Under-sampling the k-space data and reconstructing the image with sparsity constraint is one efficient way to reduce the data acquisition time. However, achieving high acceleration factor is challenging since image structures may be lost or blurred when the acquired information is not sufficient. Therefore, incorporating extra knowledge to improve image reconstruction is expected for highly accelerated imaging. Fortunately, multi-contrast images in the same region of interest are usually acquired in magnetic resonance imaging protocols. In this work, we propose a new approach to reconstruct magnetic resonance images by learning the prior knowledge from these multi-contrast images with graph-based wavelet representations. We further formulate the reconstruction as a bi-level optimization problem to allow misalignment between these images. Experiments on realistic imaging datasets demonstrate that the proposed approach improves the image reconstruction significantly and is practical for real world application since patients are unnecessarily to stay still during successive reference image scans.

Graphical abstract

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Proteolytic effects of gingipains on trefoil factor family peptides

Abstract

Objectives

The present study was aimed to determine whether trefoil factor family (TFF) peptides which were generally considered to be resistant to proteolysis could be digested by gingipains, a major proteinases produced by Porphyromonas gingivalis.

Materials and methods

Recombinant human TFF1, TFF2, and TFF3 peptides were used as substrates. Gingipains including arginine gingipain (RgpB) and lysine gingipain (Kgp) were used as enzymes. Trypsin was used as a control protease. Matrix-assisted laser desorption/ionization with time-of-flight/time-of-flight (MALDI-TOF/TOF) and liquid chromatography mass spectrometry (LC-MS) were used for analyzing peptide mass signals and amino acid sequences of digested TFF peptides.

Results

MALDI-TOF/TOF analyses demonstrated that Kgp, RgpB, and trypsin were able to cleave TFF1 and TFF2 peptides, resulting in different patterns of digested fragments. However, impurity in recombinant TFF3 peptide substrates affected the interpretations of enzymatic reaction by MALDI-TOF/TOF. LC-MS analyses demonstrated that identified fragments of TFF1, TFF2, and TFF3 from digestion by gingipains were similar to those by trypsin.

Conclusions

Using MALDI-TOF/TOF and LC-MS, the present study provides new information that gingipains containing trypsin-like activities are able to digest TFF peptides.

Clinical relevance

The proteolytic effects of gingipains on TFF peptides may be responsible for reduction of salivary TFF peptides in chronic periodontitis patients. Further investigations to determine the pathological effects of gingipains on TFF peptides in saliva and periodontal tissues of patients with chronic periodontitis would be of interest.



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The earliest evidence of coloured ornaments in China: The ochred ostrich eggshell beads from Shuidonggou Locality 2

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Publication date: December 2017
Source:Journal of Anthropological Archaeology, Volume 48
Author(s): Africa Pitarch Martí, Yi Wei, Xing Gao, Fuyou Chen, Francesco d'Errico
Beads are a communication technology used by humans to transmit information on the wearer identity to members of the same or neighbouring groups by means of a shared symbolic language. Here we focus on the earliest evidence from Eastern Asia of a communication technology – the production of artificially coloured beads – that has allowed humans to further complexify the messages conveyed by personal ornaments, and associate, to some extent, the performance characteristics of beads and pigment. We study six ochred ostrich eggshell beads (OESB) from Shuidonggou Locality 2 (SDG2), Cultural Layer (CL) 2, China, dated to c. 31kyrcalBP. Analysis of the beads manufacturing technique, size, and wear, combined with SEM-EDS and µ-Raman spectroscopy (µ-RS) study of well-preserved red residues sampled on the beads and sediment from CL2 indicate that these beads come from body ornaments and that red residues are the remnant of a pigmented compound coating the beads at the time they were worn by the SDG Palaeolithic visitors. SDG2-CL2 red stained OESBs thus represent, with the red stained bone disk from Xiaogushan, the earliest consistent evidence of pigment use for symbolic activities from China. Results also identify variations in the composition of the pigmental compound used to colour the beads that closely match differences in bead technology, size, and style. We argue that such a hitherto unknown consistency in bead production and decoration may reflect technological, morphological, and pigment coating standardisation at the individual level, standardisation in technology associated with variation in morphology and pigment at the group level, and substantial differences in these three domains at inter-group level.



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Relationship between diminution of small pulmonary vessels and emphysema in chronic obstructive pulmonary disease

Publication date: Available online 18 July 2017
Source:Clinical Imaging
Author(s): Shuko Mashimo, Shotaro Chubachi, Akihiro Tsutsumi, Naofumi Kameyama, Mamoru Sasaki, Masahiro Jinzaki, Hidetoshi Nakamura, Koichiro Asano, John J. Reilly, Tomoko Betuyaku
ObjectivesTo investigate the relationship between small pulmonary vessels and extent of emphysema on CT in individual lungs with chronic obstructive pulmonary disease (COPD).MethodsForty-nine patients were included. The percentage of cross-sectional area of vessels <5mm2 (%CSA <5) and extent of emphysema were assessed.ResultsLess than half of the COPD patients demonstrated an inverse correlation between %CSA <5 and percentage of low attenuation area (LAA%). In the remaining patients, %CSA <5 was homogeneously distributed.ConclusionNot all patients with COPD demonstrated an inverse correlation of the distributions between %CSA <5 and LAA% in individual lungs.



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The Effectiveness of a Simulation-Based Flipped Classroom in the Acquisition of Laparoscopic Suturing Skills in Medical Students—A Pilot Study

Publication date: Available online 19 July 2017
Source:Journal of Surgical Education
Author(s): Hsin-Yi Chiu, Yi-No Kang, Wei-Lin Wang, Hung-Chang Huang, Chien-Chih Wu, Wayne Hsu, Yiu-Shun Tong, Po-Li Wei
ObjectiveTo evaluate the effectiveness of a simulation-based flipped classroom in gaining the laparoscopic skills in medical students.DesignAn intervention trial.SettingTaipei Medical University Hospital, an academic teaching hospital.Participants and MethodsFifty-nine medical students participating in a 1-hour laparoscopic skill training session were randomly assigned to a conventional classroom (n = 29) or a flipped classroom approach (n = 30) based on their registered order. At the end of the session, instructors assessed participants' performance in laparoscopic suturing and intracorporeal knot-tying using the assessment checklist based on a modified Objective Structured Assessment of Technical Skills tool.ResultsStudents in the flipped group completed more numbers of stitches (mean [M] = 0.47; standard deviation [SD] = 0.507) than those in the conventional group (M = 0.10; SD = 0.310) (mean difference: 0.37; 95% CI: 0.114-582; p = 0.002). Moreover, students in the flipped group also had higher stitch quality scores (M = 7.17; SD = 2.730) than those in the conventional group (M = 5.14; SD = 1.767) (mean difference = 2.03; 95% CI: 0.83-3.228; p = 0.001). Meanwhile, students in the flipped group had higher pass rates for the second throw (p < 0.001), third throw (p = 0.002), appropriate tissue reapproximation without loosening or strangulation (p < 0.001), needle cut from suture under direct visualization (p = 0.004), and needle safely removed under direct visualization (p = 0.018) than those in the conventional group.ConclusionsComparing with traditional approach, a simulation-based flipped classroom approach may improve laparoscopic intracorporeal knot-tying skill acquisition in medical students.



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Are Residents Prepared for Surgical Cases? Implications in Patient Safety and Education

Publication date: Available online 18 July 2017
Source:Journal of Surgical Education
Author(s): Minh-Bao Mundschenk, Elizabeth B. Odom, Trina D. Ghosh, Grant M. Kleiber, Andrew Yee, Kamlesh B. Patel, Susan E. Mackinnon, Marissa M. Tenenbaum, Donald W. Buck
ObjectiveIn surgical education, the areas of focus and evaluation are skewed toward technical skill and operative knowledge; less emphasized is familiarity with the patient's medical history. The purposes of this study were to characterize how surgical trainees prepare for cases and to determine the comprehensiveness of their preparation.DesignA 27-question survey was created through a web-based software program and distributed to all resident physicians and fellows in the Departments of Surgery, Neurosurgery, and Otolaryngology at our institution. Survey responses were collected anonymously and analyzed. Institutional review board exemption was obtained.SettingThis study was performed at Washington University in St. Louis, Missouri, at an institutional hospital setting.ParticipantsThe survey was distributed to current surgical trainees at Washington University in St. Louis in the Departments of Surgery, Neurosurgery, and Otolaryngology. Further, 130 of 169 surgical residents and fellows completed the survey.ResultsMost respondents (96%) taught themselves case preparation. Only 57% of respondents reviewed the patients medical record before every surgery. Although most respondents (83%) felt they were prepared or very prepared from a patient-specific standpoint, only 24% felt that their handoff of a patient to on-call colleagues was comprehensive enough to include all pertinent aspects of a patient's history and expected perioperative course. From a technical perspective, most residents (63%) felt they were prepared or very prepared, and this level of comfort increased with postgraduate year; 76% of respondents would not feel comfortable telling their attending they were not adequately prepared.ConclusionsAlthough most trainees feel prepared or very prepared for cases from a patient-specific regard, only half review the patient's medical record before every surgery. Furthermore, almost all trainees have taught themselves how to prepare for surgery. This represents a critical gap in residency education and an opportunity to improve patient safety and quality of care.



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The Program Evaluation Committee in Action: Lessons Learned From a General Surgery Residency’s Experience

Publication date: Available online 19 July 2017
Source:Journal of Surgical Education
Author(s): Shanley B. Deal, Heather Seabott, Lily Chang, Adnan A. Alseidi
ObjectiveTo evaluate the success of the annual program evaluation process and describe the experience of a Program Evaluation Committee for a General Surgery residency program.DesignWe conducted a retrospective review of the Program Evaluation Committee's meeting minutes, data inputs, and outcomes from 2014 to 2016. We identified top priorities by year, characterized supporting data, summarized the improvement plans and outcome measures, and evaluated whether the outcomes were achieved at 1 year.SettingVirginia Mason Medical Center General Surgery Residency Program.ParticipantsProgram Evaluation Committee members including the Program Director, 2 Associate Program Directors, 2 Senior Faculty Members, and 1 Resident.ResultsAll outcome measures were achieved or still in progress at 1 year. This included purchasing a GI mentor to improve endoscopic simulation training, establishing an outpatient surgery rotation to increase the volume of cases, and implementing a didactic course on adult learning principles for faculty development to improve intraoperative teaching. Primary reasons for slow progress were lack of follow-through by delegates or communication breakdown.ConclusionsThe annual program evaluation process has been successful in identifying top priorities, developing action plans, and achieving outcome measures using our systematic evaluation process.



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Environmental scan of infection prevention and control practices for containment of hospital-acquired infectious disease outbreaks in acute care hospital settings across Canada

Publication date: Available online 18 July 2017
Source:American Journal of Infection Control
Author(s): Wrechelle Ocampo, Rose Geransar, Nancy Clayden, Jessica Jones, Jill de Grood, Mark Joffe, Geoffrey Taylor, Bayan Missaghi, Craig Pearce, William Ghali, John Conly
BackgroundWard closure is a method of controlling hospital-acquired infectious diseases outbreaks and is often coupled with other practices. However, the value and efficacy of ward closures remains uncertain.PurposeTo understand the current practices and perceptions with respect to ward closure for hospital-acquired infectious disease outbreaks in acute care hospital settings across Canada.MethodsA Web-based environmental scan survey was developed by a team of infection prevention and control (IPC) experts and distributed to 235 IPC professionals at acute care sites across Canada. Data were analyzed using a mixed-methods approach of descriptive statistics and thematic analysis.ResultsA total of 110 completed responses showed that 70% of sites reported at least 1 outbreak during 2013, 44% of these sites reported the use of ward closure. Ward closure was considered an "appropriate," "sometimes appropriate," or "not appropriate" strategy to control outbreaks by 50%, 45%, and 5% of participants, respectively. System capacity issues and overall risk assessment were main factors influencing the decision to close hospital wards following an outbreak.DiscussionResults suggest the use of ward closure for containment of hospital-acquired infectious disease outbreaks in Canadian acute care health settings is mixed, with outbreak control methods varying. The successful implementation of ward closure was dependent on overall support for the IPC team within hospital administration.



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Exposure Survey of Trends in Occupational Practice (EXPO-S.T.O.P.) 2015: A national survey of sharps injuries and mucocutaneous blood exposures among health care workers in US hospitals

Publication date: Available online 18 July 2017
Source:American Journal of Infection Control
Author(s): T. Grimmond, L. Good
BackgroundNational blood exposure (BE) surveys are valuable to health care facilities striving to reduce percutaneous sharps injuries (SIs) or mucocutaneous (MC) exposures among their health care workers (HCWs). In the Exposure Survey of Trends in Occupational Practice (EXPO-S.T.O.P.) 2015 we surveyed hospital BE incidence among members of the Association of Occupational Health Professionals in Healthcare.MethodsA 23-item electronic survey requested 2015 data on total SI and MC; SI in nurses, doctors, and surgery; staffed beds; teaching status; full time equivalent staff (FTE), nurse FTE, average daily census (ADC), and adjusted patient days (APD).ResultsOne hundred eighty-one hospitals in 34 states reported 9,343 BE (71% SI and 29% MC exposures). SI rates were 25.2/100 ADC (17.5 in nonteaching hospitals and 30.4 in teaching hospitals), 2.1/100 FTE (significantly less than that in 2001), 3.2/100 nurse FTE, 0.36/1,000 APD, and 38% occurred during surgery. MC exposure incidence rates were 10.5/100 ADC (8.6 in nonteaching hospitals and 11.7 in teaching hospitals), 0.86/100 FTE, and 0.14/1000 APD.ConclusionsBE incidence rates have fallen slowly but significantly since 2001, but the reduction is far less than hoped. Occupied beds (ie, ADC) has become a less-useful denominator. We estimate more than 300,000 HCWs sustain SIs annually in hospital and nonhospital settings. Greater resources are needed for more frequent and correct use of safety devices, training to competency, and root-cause investigation of all SIs.



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Letter to the editor on “Antimicrobial copper alloys decreased bacteria on stethoscope surfaces”

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Publication date: Available online 18 July 2017
Source:American Journal of Infection Control
Author(s): Marko Predic, Stefanie Buchanan




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Antimicrobial stewardship in the treatment of skin and soft tissue infections

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Publication date: Available online 18 July 2017
Source:American Journal of Infection Control
Author(s): Julie A. Gibbons, Hayden L. Smith, Sudhir C. Kumar, Katherine Johnson Duggins, Amanda M. Bushman, Jayme M. Danielson, William J. Yost, Jonathan J. Wadle
BackgroundResearch on treating skin and soft tissue infections (SSTI) has shown improved patient outcomes with effective pharmaceutic prescribing. Antimicrobial stewardship programs can reduce consequences of broad-spectrum antimicrobial administration in SSTI treatment.MethodsProspective and historic control data were collected during two 7-month periods. Intervention consisted of implementing a new SSTI evidence-based treatment algorithm and provider education, including calls and medical record notes targeted at physicians.ResultsOf 412 patients, 76 and 86 were found eligible from the historic and intervention groups, respectively. The intervention group had a higher prevalence of appropriate antibiotic usage (33% vs 19%, respectively; P = .04). There was a lower median number of days from intravenous antibiotic therapy to oral conversion (3 vs 5; P < .0001) and a lower median number of days of antipseudomonal antibiotic use (3 vs 5; P = .03) in the intervention group, respectively. The intervention group also had fewer documented SSTI treatment complications (1% vs 8%, respectively; P = .04). The positive outcomes outlined demonstrate potential impacts made from the use of multidisciplinary antibiotic stewardship initiatives.ConclusionsAppropriate use of antimicrobial agents under the direction of an antimicrobial stewardship program can lead to improved outcomes for patients being treated for SSTIs.



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A proposal for appropriate countermeasures to infectious diseases in schools

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Publication date: Available online 19 July 2017
Source:American Journal of Infection Control
Author(s): Seok-Ju Yoo




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What do visitors know and how do they feel about contact precautions?

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Publication date: Available online 18 July 2017
Source:American Journal of Infection Control
Author(s): Grace Seibert, Tola Ewers, Anna K. Barker, Adam Slavick, Marc-Oliver Wright, Linda Stevens, Nasia Safdar
Patients with Clostridium difficile infection (CDI) are placed in contact precautions. We surveyed 31 visitors of CDI patients to understand their compliance, knowledge, and perceptions of contact precautions. Although most visitors knew where to find the required personal protective equipment, only 42% were fully compliant with gown and gloves. Family members accounted for 90% of visitors, and roughly half of the reasons given for not gowning were related to a lack of perceived risk for family members. Nursing staff are fundamental sources of personal protective equipment (PPE) information for visitors; however, we found variation in staff communication regarding need for visitor PPE use.



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Turbocharging vaccines: emerging adjuvants for dendritic cell based therapeutic cancer vaccines

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Publication date: August 2017
Source:Current Opinion in Immunology, Volume 47
Author(s): Mansi Saxena, Nina Bhardwaj
Development of therapeutic cancer vaccines has been hindered by the many pro-tumorigenic mechanisms at play in cancer patients that serve to suppress both antigen presenting cells and T cells. In face of these obstacles, cancer vaccines are most likely to promote anti-tumorigenic immune responses only when formulated with strong adjuvants, and in combination with new immune interventions designed to reverse immune suppression and exhaustion of T cells in the tumor microenvironment. Dendritic cells (DCs) are often termed 'nature's adjuvant' due to their exceptional capacity for initiating both innate and adaptive immune responses. Hence, the past decade has witnessed a flurry of activity in testing DC based immunotherapies for cancer intervention. In this review we will discuss advances in conventional adjuvants and provide insight into new adjuvants as they pertain to DC cancer therapy.



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Old and new adjuvants

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Publication date: August 2017
Source:Current Opinion in Immunology, Volume 47
Author(s): Amy S McKee, Philippa Marrack
Adjuvants have been deliberately added to vaccines since the 1920's when alum was discovered to boost antibody responses, leading to better protection. The first adjuvants were discovered by accident and were used in the safer but less immunogenic subunit vaccines, supposedly by providing an antigen depot to extend antigen presentation. Since that time, much has been discovered about how these adjuvants impact cells at the tissue site to activate innate immune responses, mobilize dendritic cells and drive adaptive immunity. New approaches to vaccine construction for infectious diseases that have so far not been well addressed by conventional vaccines often attempt to induce antibodies, polyfunctional CD4+ T cells and CD8+ CTLs. The discovery of pattern recognition receptors and ligands that drive desired T cell responses has led to development of novel adjuvant strategies using immunomodulatory agents to direct appropriate immune responses.



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CD8+ T cell programming by cytomegalovirus vectors: applications in prophylactic and therapeutic vaccination

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Publication date: August 2017
Source:Current Opinion in Immunology, Volume 47
Author(s): Klaus Früh, Louis Picker
Vectors based on cytomegalovirus (CMV) represent a novel vaccine platform that maintains high frequencies of non-exhausted effector memory T cells in both CMV sero-positive and sero-negative individuals. In non-human primate models, CMV vectored vaccines provide unprecedented protection against simian immunodeficiency virus (SIV). Moreover, CMV vectors can be genetically altered to program highly diverse CD8+ T cell responses that differ in their epitope targeting including conventional, MHC-I restricted CD8+ T cells as well as unconventional CD8+ T cells restricted by MHC class II or non-polymorphic MHC-E. By modifying cytomegaloviral determinants that control unconventional T cell priming it is possible to uniquely tailor the CD8+ T cell response for each individual disease target in order to maximize prophylactic or therapeutic protection.



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