Genotoxicity of inhalational anesthetics and its relationship with the polymorphisms of GSTT1, GSTM1, and GSTP1 genes

Abstract

Due to their wide applications, concern exists regarding possible genotoxic effects of inhalational anesthetics (IAs) among operating room personnel. This study was undertaken to examine genotoxic properties of co-exposure to nitrous oxide, sevoflurane, and isoflurane on induction of micronucleus (MN) and chromosomal aberrations (CAs) and to determine whether any associations exist between polymorphisms of GST genes and the level of genomic damage measured by MN and CAs assays. Sixty operating room personnel and 60 unexposed referent nurses were studied. The workers' exposure to the IAs was determined. DNA damage was evaluated by MN and CAs assays. Additionally, the GSTM1, GSTT1, and GSTP1 polymorphisms were detected. The mean concentrations of nitrous oxide, isoflurane, and sevoflurane were found to be 850.92 ± 919.78, 2.40 ± 0.86, and 0.18 ± 0.14 ppm, respectively. The frequency of MN and CAs in the exposed group was significantly higher than that of the non-exposed group. The frequency of MN was significantly higher in referent nurses with null GSTT1, compared to referent nurses with positive GSTT1. The frequency of MN was significantly higher in exposed individuals carrying the combined genotype of GSTT1 (−), GSTM1 (−), and GSTP1 AG as compared with subjects carrying a combination of GSTT1 (+), GSTM1 (+), and GSTP1 AA. Statistically significant associations were noted between exposure to the IAs, gender, and the combination of the three GSTs genotypes with MN frequency. These findings indicate that inhalation exposure to IAs induces genotoxic response and the polymorphisms of GSTs genes might modulate the effect of exposure to IAs on MN.

https://ift.tt/2KWwwA2

Assessment of sediment capping with zirconium-modified bentonite to intercept phosphorus release from sediments

Abstract

Three different types of zirconium-modified bentonites (ZrMBs) including zirconium-modified original bentonite (ZrMOB), zirconium-modified magnesium-pretreated bentonite (ZrMMgB), and zirconium-modified calcium-pretreated bentonite (ZrMCaB) were synthesized and used as active covering materials to suppress the release of phosphorus (P) from sediments. To assess the covering efficiency of ZrMBs to inhibit P release from sediments, we examined the impact of ZrMB covering layer on P mobilization in sediments at different depths as well as the release of P through the interface between sediment and overlying water (SWI) by use of simulating P release control experiments and diffusive gradients in thin films (DGT) technology. The results showed that the amount of soluble reactive P (SRP) in the overlying water greatly decreased after covering with ZrMBs. Moreover, both pore water SRP and DGT-liable P (DGT-P) in the top sediments decreased after capping with ZrMBs. An obvious stratification of DGT-P was observed along the vertical direction after covering with ZrMBs, and static and active layers were found in the top sediment and in the lower sediment directly below the static layer, respectively. Furthermore, ZrMB covering led to the change of P species from easily released P to relatively or very stable P, making P in the top sediment more stable compared to that without ZrMB covering. Besides, an overwhelming majority of P immobilized by ZrMBs is hard to be re-released into the water column in a common environment. Overall, the above results demonstrate that sediment covering with ZrMBs could effectively prevent the transport of SRP from sediments into the overlying water through the SWI, and the control of P transport into the overlying water by ZrMB covering could be mostly due to the immobilization of pore water SRP, DGT-P, and mobile P in the top sediment by ZrMBs.

https://ift.tt/2BSAqap

Managing pitted keratolysis: consider topical glycopyrrolate

https://ift.tt/2Sw99jC

Efficacy and safety of oral atenolol for the treatment of infantile haemangioma: A systematic review

Abstract

Recently, several studies have reported their experience in using oral atenolol in patients with infantile haemangioma (IH), especially as an alternative to propranolol, but the efficacy and safety of oral atenolol has not been evaluated. We searched PubMed (Medline), Central, Embase, Web of Science and EBSCOhost (until May 2018) for the eligible studies reporting more than 10 IH patients who were treated with oral atenolol with detailed original data, including outcomes, regimens and adverse events (AEs). The data was standardised and analysed by using R software with meta‐package. A total of 9 of 141 identified articles, including 341 infantile haemangioma patients treated with oral atenolol therapy, were included. The pooled response rate of atenolol was 0.90 (95% CI: 0.85–0.93), and the rebound rate was 0.11 (95% CI: 0.08–0.16). Among the 341 patients, 44 patients were switched to atenolol therapy from propranolol due to adverse events. The response rate of subsequent atenolol treatment was 90.9% (40/44). Regarding AEs, 141 patients reported 177 episodes of AEs, and the pooled rate was 0.26 (95% CI: 0.12–0.47). Gastrointestinal symptoms (e.g. constipation, diarrhoea and vomiting) were the most frequent AEs (22.6%). Widely known propranolol‐related AEs, including hypoglycaemia, bronchospasm, bradycardia and hypotension, were not recorded. Overall, atenolol appears to be an effective and safe therapy for the treatment of IH and may be a promising alternative to propranolol.

https://ift.tt/2EfUS7D

Heavy metals in organs of stray dogs and cats from the city of Naples and its surroundings (Southern Italy)

Abstract

The aim of the present study was to assess the concentrations of lead (Pb) and cadmium (Cd) in the liver and kidney from stray dogs (Canis lupus familiaris) and cats (Felis catus) found dead between 2014 and 2017 in the city of Naples and its surrounding areas. These organs from 290 dogs and 88 cats were collected after ordinary necropsy of stray animals. Heavy metal concentrations were determined by using atomic absorption spectrophotometry (AAS). Concentrations of Pb (up to 5.93 mg/kg) and Cd (ranging from 0.005 to 6.13 mg/kg) were detected in both livers and kidneys analyzed. Differences in concentration were found based on age class, gender, and kind of tissue for both elements, with a trend similar to those already reported in the literature for comparable studies from different countries. Cadmium levels in the kidney were significantly higher (p < 0.05) in females than those in males for both species. As regards to Pb, the highest concentrations were detected in the liver (3.45 mg/kg in dog and 5.93 in cat, respectively) followed by the renal tissue, with no significant difference depending on the animal gender. This study can be considered the first one in Italy regarding stray dogs and cats as bio-indicators of environmental contamination due to lead and cadmium, suggesting that pets could be sentinel animals to evaluate human exposure to these heavy metals.

https://ift.tt/2Pmdynn

Changes of DNA methylation of Isoetes sinensis under Pb and Cd stress

Abstract

To investigate the molecular response of ancient plants to heavy metal stress and to explore the feature of DNA methylation in endangered plants after exposure to heavy metals, the Isoetes sinensis, an endangered plant, was stressed with three different concentrations of two heavy metals lead (Pb) and cadmium (Cd), respectively. Then the degrees and the patterns of DNA methylation in the leaves were measured on the 14th day using Methylation Sensitive Amplified Polymorphism (MSAP) technique. The results showed that the DNA methylated profile of I. sinensis was affected by Pb and Cd stress. There was no significant difference in the amount of DNA methylation among control check (CK), Pb stress group, and Cd stress group (CK 46.96%, Pb 48.23%, and Cd 48.1%). However, full-methylation level of Pb stress group (28.34%) and Cd stress group (20.25%) was lower than control (33.91%), in contrast, hemi-methylation level Pb stress group (19.89%) and Cd stress group (27.85%) were higher than control (13.04%). The change of patterns from no methylation or hemi-methylation of internal and external cytosines into full-methylation of internal and external cytosines accounted for a large proportion in enhanced methylation aspects. The full-methylation into no methylation or hemi- or full-methylation of internal and external cytosines occupied most of demethylation. The proportion of DNA methylation (including hypermethylation) by both Pb and Cd stresses is nearly equal (39.04% and 39.71%), but the proportion of DNA demethylation by Cd is higher than that by Pb (46.86% than 33.92%).

https://ift.tt/2zJS5Q7

Nitrate removal from groundwater using negatively charged nanofiltration membrane

Abstract

A commercial nanofiltration (NF) membrane was modified using poly(sodium 4-styrenesulfonate) (PSS) to improve the nitrate rejection from groundwater. Fourier transform infrared spectroscopy, thermogravimetric analysis, zeta potential, and water contact angle analyses were performed, showing that PSS was successfully coated onto the membrane with the surface negative charge density being enhanced. The results of nitrate removal tests showed that the best PSS concentration was 1.5 mg/L, with the nitrate rejection rate of 88.8% and the permeate flux of 27.0 L/m² h. The effect of initial nitrate concentration and solution pH on the nitrate removal performance of the modified NF membrane was investigated. The results indicate that the modified NF membrane can improve nitrate removal from actual groundwater, with little membrane permeate flux loss.

Graphical abstract

https://ift.tt/2Pmdsw1

Residue behavior and risk assessment of cymoxanil in grape under field conditions and survey of market samples in Guangzhou

Abstract

A simple and fast method based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for cymoxanil residue analysis in grape. Sample preparation based on solid-liquid extraction was optimized without using adsorbent for purification. Recoveries were 79.8–109.5% with relative standard deviations (RSDs) of 2.5–9.4% at fortified levels from 0.001 to 0.50 mg/kg. The limit of detection (LOD) was 0.3 μg/kg. Field trials were conducted to explore the dissipation and terminal residue behavior of cymoxanil in grape. Results showed that the half-lives of cymoxanil were from 0.5 to 0.7 days. Terminal residues were from below the limit of quantification (LOQ) to 0.363 mg/kg. Dietary exposure risk assessment revealed that the risk quotients (RQs) were much less than 1. It was concluded that cymoxanil in grape raised negligible concerns to human health under field conditions. Sixty grape samples from Guangzhou market were found to be free of cymoxanil. The proposed study would provide reference for appropriate use of cymoxanil in grape planting in China.

https://ift.tt/2zRFHOf

Chemical analysis of in vivo –irradiated dentine of head and neck cancer patients by ATR-FTIR and Raman spectroscopy

Abstract

Objectives

To evaluate the effect of in vivo radiotherapy on the chemical properties of human dentine by Fourier-transform infrared spectroscopy (FTIR) and Raman analysis.

Materials and methods

Chemical composition was evaluated comparing control and irradiated group (n = 8). Irradiated teeth were obtained from radiotherapy patients subjected to fractionated X-ray radiation of 1.8 Gy daily totaling 72 Gy. The teeth were sectioned according to the type of dentine (crown or root dentine), obtaining 3-mm dentine cervical slices. The analyzed parameters by FTIR and Raman spectroscopies were mineral/matrix ratio (M:M), carbonate/mineral ratio (C:M), amide I/amide III ratio, and amide I/CH₂ ratio. Raman also calculated the phosphate and carbonate crystallinity.

Results

FTIR revealed that M:M had a decrease in both factors (p = 0.008; p = 0.043, respectively) and root dentine showed a lower C:M in the irradiated group (p = 0.003). Raman revealed a higher phosphate crystallinity and a lower carbonate crystallinity in crown dentine of irradiated group (p = 0.021; p = 0.039). For amide I/amide III, the irradiated showed a lower ratio when compared to the control group (FTIR p = 0.002; Raman p = 0.017). For amide I/CH₂, the root dentine showed a higher ratio than the crown dentine in both methods (p < 0.001).

Conclusions

Radiotherapy altered the chemical composition of human dentine. The exchange of phosphate-carbonate ions in the hydroxyapatite and higher concentration of organic components was found after radiotherapy.

Clinical relevance

The increased risk of radiation-related caries in patients undergoing head and neck radiotherapy is due not only to salivary, dietary, and microbiological changes but also to changes in tooth chemical composition.

https://ift.tt/2Uh0eUH

The effect of thyroid functions on osteopenia of prematurity in preterm infants

Journal Name: Journal of Pediatric Endocrinology and Metabolism
Issue: Ahead of print


https://ift.tt/2rjOzHu

Use of antiosteoporotic drugs and calcium/vitamin D in patients with fragility fractures: impact on re-fracture and mortality risk

Abstract

Purpose

To evaluate the impact of pharmacological treatment in osteoporosis patients with recent fracture and to assess the incidence of subsequent fracture and all-cause mortality.

Methods

This observational retrospective study was based on data from administrative databases of five Italian Local Health Units. Osteoporosis patients aged ≥ 50 years with hospitalization for vertebral or hip fracture occurring between 01/01/2011 and 31/12/2015 were included. Treatment adherence was calculated using the medication possession ratio. Multivariable proportional hazard Cox model was used to identify factors associated with time to re-fracture and all-cause mortality.

Results

A cohort of 3475 patients were included and 41.5% of them did not receive any specific anti-fracture treatment. Among treated patients (N = 2032), the majority (83.6%) received calcium/vitamin D supplementation. Over a mean follow-up of 3 years, the risk of subsequent fractures was 44.4% lower in treated patients compared to untreated ones (HR = 0.556, 95% CI = 0.420–0.735, p < 0.001) and 64.4% lower in those receiving calcium/vitamin D supplementation compared to osteoporosis treatment only (HR = 0.356, 95% CI = 0.237-0.533, p < 0.001). The risk of re-fracture was 77.2% lower in treated patients who were adherent to medication (HR = 0.228, 95% CI = 0.139–0.376, p < 0.001). Treated patients had 64% lower mortality risk over the follow-up compared to untreated ones (HR = 0.360, 95% CI = 0.310–0.418, p < 0.001).

Conclusions

A consistent proportion of osteoporosis patients did not receive specific treatment after a fracture, showing poor adherence to national guidelines on osteoporosis treatment. Osteoporosis drug treatment, and to a greater extent in combination with calcium/vitamin D, and adherence were correlated with lower risk of both re-fracture and all-cause mortality.

https://ift.tt/2PhD64N

Use of antiosteoporotic drugs and calcium/vitamin D in patients with fragility fractures: impact on re-fracture and mortality risk

Abstract

Purpose

To evaluate the impact of pharmacological treatment in osteoporosis patients with recent fracture and to assess the incidence of subsequent fracture and all-cause mortality.

Methods

This observational retrospective study was based on data from administrative databases of five Italian Local Health Units. Osteoporosis patients aged ≥ 50 years with hospitalization for vertebral or hip fracture occurring between 01/01/2011 and 31/12/2015 were included. Treatment adherence was calculated using the medication possession ratio. Multivariable proportional hazard Cox model was used to identify factors associated with time to re-fracture and all-cause mortality.

Results

A cohort of 3475 patients were included and 41.5% of them did not receive any specific anti-fracture treatment. Among treated patients (N = 2032), the majority (83.6%) received calcium/vitamin D supplementation. Over a mean follow-up of 3 years, the risk of subsequent fractures was 44.4% lower in treated patients compared to untreated ones (HR = 0.556, 95% CI = 0.420–0.735, p < 0.001) and 64.4% lower in those receiving calcium/vitamin D supplementation compared to osteoporosis treatment only (HR = 0.356, 95% CI = 0.237-0.533, p < 0.001). The risk of re-fracture was 77.2% lower in treated patients who were adherent to medication (HR = 0.228, 95% CI = 0.139–0.376, p < 0.001). Treated patients had 64% lower mortality risk over the follow-up compared to untreated ones (HR = 0.360, 95% CI = 0.310–0.418, p < 0.001).

Conclusions

A consistent proportion of osteoporosis patients did not receive specific treatment after a fracture, showing poor adherence to national guidelines on osteoporosis treatment. Osteoporosis drug treatment, and to a greater extent in combination with calcium/vitamin D, and adherence were correlated with lower risk of both re-fracture and all-cause mortality.

https://ift.tt/2PhD64N

Primary Cutaneous vs Secondary Cutaneous Follicular Lymphomas: A Comparative Study Focused on BCL2, CD10 and t (14;18) Expression

Background

Primary cutaneous follicular center‐cell lymphoma (PCFCL) is one of the most common types of cutaneous B‐cell lymphoma. Differences in immunohistochemical expression of BCL2 and CD10 antigens along with the presence of t(14:18) translocation in neoplastic cells have been postulated as relevant clues in differentiating PCFCL from cutaneous lesions secondary to a systemic follicular lymphoma (SCFL). The aim of this study is to evaluate the significance and usefulness of these parameters in a large series of patients.

Methods

Patients with PCFCL and SCFL diagnosed at three University Hospitals in Barcelona, from 2000‐2015 were reviewed. Clinical, histopathological, immunophenotypical, genetic and outcome parameters were analysed

Results

Eighty‐one cases (59 PCFCL and 22 SCFL) were included. There were no significant differences between PCFCL and SCFL cases regarding clinical presentation, site of involvement or predominant type of skin lesions. Most patients in both groups showed positivity for BCL2 and CD10 but strong expression of BCL2 and CD10 were associated with SCFL cases. Although more frequent in SCFL, a small proportion of PCFCL cases also showed the t (14:18) on FISH analysis.

Conclusions

The intensity of BCL2 expression was found to be the single most valuable clue in differentiating PCFCL from SCFL cases on histopathological grounds.

This article is protected by copyright. All rights reserved.

https://ift.tt/2SuNhoS

A diagnostically‐challenging case of melanoma ex blue nevus with comprehensive molecular analysis, including the 23‐gene expression signature (myPath Melanoma)

Melanoma ex blue nevus (MEBN) is a rare, aggressive, and potentially lethal neoplasm. Distinguishing MEBN from an atypical cellular blue nevus can be very challenging. We report a diagnostically difficult case of MEBN with lymph node metastases, in which single nucleotide polymorphism array and fluorescence in situ hybridization were used to arrive at the correct diagnosis. It was also analyzed by the recently‐introduced proprietary 23‐gene expression signature test (myPath Melanoma, Myriad Genetics Inc.). To our knowledge, this is the second reported case of MEBN analyzed by the 23‐gene expression signature, which provided a false negative result. More studies are needed to assess the sensitivity and specificity of this test in various melanocytic proliferations.

This article is protected by copyright. All rights reserved.

https://ift.tt/2EfNRnp

Ibrutinib plus rituximab superior to standard treatment for some patients with chronic leukemia

A clinical trial showed that ibrutinib plus rituximab is superior to standard treatment for patients age 70 and younger with untreated chronic lymphocytic leukemia (CLL). Findings were announced at the American Society of Hematology annual meeting.

https://ift.tt/2BRZezr

18F-Fluorocholine for the Detection of Parathyroid Adenomas

Condition:   Hyperparathyroidism, Primary
Intervention:   Drug: Fluorocholine PET
Sponsor:   University of California, San Francisco
Recruiting

https://ift.tt/2zKaoof

Self-targeted knockdown of CD44 improves cisplatin sensitivity of chemoresistant non-small cell lung cancer cells

Abstract

Background

Chemoresistance remains a major challenge for effective chemotherapy of non-small-cell lung carcinoma (NSCLC). CD44 expression is related to the susceptibility of various cancer cell types to anticancer drugs. Here, we systematically investigated the CD44-dependent chemoresistance of NSCLC cells and developed a liposomal siRNA delivery system to overcome this chemoresistance by the self-targeted downregulation of CD44.

Methods

We confirmed the relationship between the expression of CD44 and the chemosensitivity of NSCLC cells using flow cytometry and MTT assay. We then generated and characterized cisplatin-resistant cell lines and compared the expression of CD44 in resistant cells to that in parental cells using western blotting. To evaluate whether the chemosensitivity of resistant cells depends on CD44 expression, we performed CD44 knockdown using CD44 siRNA and detected the chemosensitivity of these cells. Additionally, we prepared hyaluronic acid (HA)-coated liposomes as a targeted delivery system to selectively deliver CD44-specific siRNA to chemoresistant NSCLC cells and observed whether the chemosensitivity of these cells was improved.

Results

We found that CD44 expression is inversely proportional to the degree of cellular response to cisplatin chemotherapy and that CD44 is overexpressed in chemoresistant NSCLC cells. By performing CD44 knockdown using siRNA, we reconfirmed that the chemosensitivity of resistant cells depends on CD44 expression. We also observed that HA-liposome-mediated siRNA delivery prior to cisplatin chemotherapy significantly reduced CD44 expression and enhanced cisplatin sensitivity in chemoresistant NSCLC cells.

Conclusions

These results suggest that self-targeted downregulation of chemoresistance-associated cell surface proteins during chemotherapy is an effective therapeutic strategy for overcoming the chemoresistance of NSCLC cells.

https://ift.tt/2zHUAT4

Lack of UGT polymorphism association with idasanutlin pharmacokinetics in solid tumor patients

Abstract

Purpose

Idasanutlin is a selective small-molecule MDM2 antagonist. It activates the tumor suppressor TP53 and is in phase 3 clinical trial for acute myeloid leukemia. Nonclinical studies have shown that glucuronidation is the major metabolizing mechanism for idasanutlin and UGT1A3 is the major metabolizing enzyme. There are reported examples of UGT polymorphisms associated with drug metabolism or response. Thus, the aim of this analysis is to investigate if UGT polymorphism is associated with idasanutlin pharmacokinetics.

Method

Idasanutlin clearance was derived and normalized from two phase I studies. Its clearance level was compared between patients with different genotypes at 44 non-monomorphic UGT SNPs. Several single-locus and multi-locus association analysis, including haplotype association analysis and pairwise SNP interaction (epistasis) analyses were performed to investigate if there is any association between UGT genotypes and idasanutlin clearance.

Results and conclusion

A total of 69 patients who have both idasanutlin pharmacokinetic data and UGT genotyping data were analyzed for association. The major clearance enzyme for idasanutlin, UGT1A3, has no association with idasanutlin clearance. Further single-locus and multi-locus association analyses also suggest that no significant UGT polymorphism association with idasanutlin clearance can be detected with the current datasets. However, the possibility of association with rare allele(s) of UGT family genes cannot be excluded due to the limited sample size of the current phase I studies.

https://ift.tt/2Ru4Hl7

A retrospective study of the CHOP, CHOPE, and CHOPE/G regimens as the first-line treatment of peripheral T-cell lymphomas

Abstract

Purpose

The standard treatment for peripheral T-cell lymphomas (PTCLs) is undetermined. We designed a CHOPE/G regimen (cyclophosphamide, pirarubicin, vincristine, prednisolone, and etoposide alternating with a gemcitabine-based regimen) as the first-line treatment of PTCLs and compared with CHOP (cyclophosphamide, pirarubicin, vincristine, and prednisolone) and CHOPE (CHOP plus etoposide) regimen to evaluate the optimal chemotherapy regimen.

Methods

116 previously untreated PTCL patients received CHOP (N = 46), CHOPE (N = 46), or CHOPE/G (N = 24) regimen at Peking University Cancer Hospital from 2009 to 2017 and were retrospectively analyzed.

Results

The overall response rates (ORRs) of the CHOP, CHOPE, and CHOPE/G groups were 82.6%, 76.1%, and 75.0% (p = 0.673), with complete response (CR) rates of 32.6%, 56.5%, and 45.7% (p = 0.063), respectively. Within a median follow-up time of 35.5 months, the 3-year overall survival (OS) rates of the CHOP, CHOPE, and CHOPE/G groups were 37.0%, 47.0%, and 56.3% (p = 0.107), and the 3-year progression-free survival (PFS) rates were 19.9%, 29.9%, and 5.3% (p = 0.093), respectively. Compared with the CHOP regimen alone, CHOPE had a significantly higher CR rate (p = 0.021) with more favorable OS (p = 0.046). The CHOPE/G regimen did not improve the ORR, CR rate, or OS compared with either the CHOP or CHOPE, with a significantly poorer PFS compared with the CHOPE regimen (p = 0.029). Anemia and thrombocytopenia occurred most frequently in the CHOPE/G group (anemia 83.3%, p = 0.035; thrombocytopenia 50%, p = 0.015).

Conclusions

Compared with CHOP alone, CHOPE regimen improved the efficacy and survival; while the addition of gemcitabine in the front-line therapy resulted in more adverse events without benefit of survival.

https://ift.tt/2rhYuNZ

Severe hypersensitivity reactions to platinum compounds post-pressurized intraperitoneal aerosol chemotherapy (PIPAC): first literature report

Abstract

Background

Pressurized intraperitoneal aerosol chemotherapy (PIPAC) shows encouraging results for patients with unresectable peritoneal metastasis. Several reports demonstrated the safety of the procedure combined with systemic chemotherapy, with a low rate of complication. The aim of this study is to report severe hypersensitivity reactions to platinum compounds (SHRPC) during PIPAC procedures.

Methods

All patients who underwent PIPAC for non-resectable PC in Lyon Sud University hospital were included in a prospective institutional database. All patients who presented a SHRPC after PIPAC were included in our analysis.

Results

One hundred and thirty-two patients underwent 383 PIPAC procedures between December 2015 and December 2017. oxaliplatin's and cisplatin–doxorubicin's protocols were used in 71 and 312 PIPAC, respectively. Four patients (3%) developed SHRPC; two patients (2.8%) after oxaliplatin and two patients (0.6%) after cisplatin–doxorubicin protocols. SHRPC occurred during the 6th PIPAC with cisplatin–doxorubicin protocol and during 2nd and 3rd PIPAC of the oxaliplatin protocol. Three events appeared within 15 min and one event occurred 50 min following nebulization. All the SHRPC have been managed successfully without any complication.

Conclusions

This is the first report of SHRPC after PIPAC. The physician must constantly keep this rare but life-threatening complication in mind, especially after repeated PIPAC administration or previous platinum-based systemic chemotherapy.

https://ift.tt/2FTnm8A

A phase II trial of EGFR-TKI readministration with afatinib in advanced non-small-cell lung cancer harboring a sensitive non-T790M EGFR mutation: Okayama Lung Cancer Study Group trial 1403

Abstract

Purpose

The aim of this study was to evaluate the efficacy of epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitor (TKI) readministration using afatinib in patients with non-small-cell lung cancer (NSCLC) with a sensitive non-T790M EGFR mutation who had received cytotoxic chemotherapy after acquiring resistance to EGFR-TKIs.

Methods

Eligible patients had EGFR-mutant tumors resistant to first- or second-generation EGFR-TKIs and an EGFR-TKI-free period with cytotoxic agents. Confirmation of absence of the T790M mutation was required before registration. Afatinib (40 mg/body) was administered daily. The primary endpoint was progression-free survival (PFS). We assumed estimated and threshold PFS times of 3.3 and 1 months, with an α of 0.05 and β of 0.1, respectively.

Results

Twelve patients were enrolled from December 2014 to May 2017. The objective response rate and disease control rate were 17% and 84%, respectively. The median PFS time was 4.2 months (95% confidence interval [CI] 2.0–5.8), which met the pre-defined primary endpoint. The median overall survival was 11.6 months (95% CI 9.2-not reached). Grade 3 or worse adverse events included diarrhea (25%), elevated creatinine levels (8%), and hypokalemia (8%), without any treatment-related deaths.

Conclusion

EGFR-TKI readministration with afatinib for sensitive EGFR-mutant NSCLC without T790M after resistance to a first- or second-generation EGFR-TKI yielded modest activity with tolerable toxicity. It might be one of the treatment options in patients who do not possess T790M tumors, although further studies in this patient setting are warranted.

https://ift.tt/2zIbIZf

Lactobacillus supplementation prevents cisplatin-induced cardiotoxicity possibly by inflammation inhibition

Abstract

Purpose

Cardiotoxicity of chemotherapy exerts as the main hurdle for prognosis, while whether cisplatin causes severe cardiotoxicity remains largely unknown. Accumulating evidence reveals that intestinal microbiota functions importantly in nutrient metabolism and cardiovascular function. In this study, we observed the possible cardiotoxicity of cisplatin and explored the possible role of microbiota in the mouse model.

Methods

C57Bl6 mice were treated with 0, 3 or 6 mg/kg cisplatin via i.p. injection, together with or without Lactobacillus supplementation. Cardiac function was analyzed by echocardiography. Gut microbiota was analyzed by 16S RNA sequencing. Gene expression was analyzed by qPCR. The data differences were compared with Graphpad Prism 7.0.

Results

In comparison with the control group, 6 mg/kg per week cisplatin treatment for 3 weeks significantly decreased the body weight by about 33% (18.1 ± 2.1 vs 27.2 ± 0.9) and decreased the left ventricular ejection fraction by about 15% (0.57 ± 0.07 vs 0.67 ± 0.04). Together, the gut microbiota was found dramatically changed, manifested as 27% decrease of Firmicutes and increased pathological bacteria. Antibiotics treatment had no obvious beneficial effects on the body weight and cardiac function caused by cisplatin. However, Lactobacillus supplementation significantly increased the body weight and restored cardiac function, together with lower inflammation gene expression.

Conclusions

The study here has established a possible role of microbiota dysbiosis in cisplatin-associated toxic effects, while delivery of Lactobacillus would be beneficial for the cardiac function prevention possibly via inflammation control.

https://ift.tt/2y3HmOM

Comment on: Yohshino et al.: combination chemotherapy with irinotecan and gemcitabine for taxane/platinum-resistant/refractory ovarian and primary peritoneal cancer: a multicenter phase I/II trial (GOGO-Ov 6). Cancer Chemother Pharmacol (2017) 80:1239–1247

https://ift.tt/2HueLtt

Oncogenic roles of serine–threonine kinase receptor-associated protein (STRAP) in osteosarcoma

Abstract

Purpose

To validate the presence of serine–threonine kinase receptor-associated Protein (STRAP) in osteosarcoma tissue and to investigate the oncological role of STRAP in osteosarcoma.

Methods

Expression of STRAP protein in osteosarcoma tissue compared to soft callus (hyperactive bone healing tissue) and in multiple cell lines was examined using western blot analysis. Effects of STRAP silencing on cell proliferation, invasion, migration and re-implantability in chick chorioallantoic membrane (CAM) were observed in osteosarcoma cell lines (MNNG-HOS, 143B, and U2OS).

Results

The result demonstrated that STRAP was highly up-regulated in osteosarcoma tissues compared with the normal physiological bone healing tissue (soft callus). Expression level of STRAP was markedly high in osteosarcoma cell lines with aggressive phenotype. Upon STRAP silencing, invasion and migration, but not proliferative activity, were selectively modulated in high-expression-STRAP cell lines. In addition, STRAP silencing reduced the success rate of tumor implantation and growth of MNNG-HOS cells in CAM model.

Conclusions

Serine–threonine kinase receptor-associated protein is up-regulated during osteosarcoma progression. The presence of STRAP enhances osteosarcoma cell invasion, migration and re-implantation ability, factors which play a critical role in metastasis. Serine–threonine kinase receptor-associated protein and its related pathway are worthy for further exploration as a novel target for anti-metastasis agents.

https://ift.tt/2OpA7uB

Enhancing 5-fluorouracil efficacy through suppression of PKM2 in colorectal cancer cells

Abstract

Purpose

Cancer cells alter regular metabolic pathways in order to sustain rapid proliferation. One example of metabolic remodeling in cancerous tissue is the upregulation of pyruvate kinase isoenzyme M2 (PKM2), which is involved in aerobic glycolysis. Indeed, PKM2 has previously been identified as a tumor biomarker and as a potential target for cancer therapy. Here, the role of PKM2 in the anticancer efficacy of 5-fluorouracil (5-FU) was evaluated in colorectal cancer (CRC).

Methods

HCT116, SW480 and HT-29 cells were used by transfection with lentiviral vectors expressing short hairpin RNA (shRNA) against PKM2. In response to 5-FU treatment, cellular proliferation was examined, the levels of ATP/ADP ratio were monitored, the intracellular accumulation of 5-FU was measured, and intracellular levels of phosphoenolpyruvate (PEP), pyruvate and lactate were evaluated by using liquid chromatography–mass spectrometry (LC–MS). A CRC subcutaneous tumor model was performed to investigate the effect of PKM2 inhibition on 5-FU efficacy in vivo.

Results

Suppression of PKM2 resulted in changes in glucose metabolism, leading to decreased synthesis of adenosine triphosphate (ATP). Reduced levels of ATP/ADP ratio resulted in the intracellular accumulation of 5-FU, consequently enhancing the therapeutic efficacy of this drug in several CRC cell lines. Furthermore, the enhanced efficacy of 5-FU by simultaneous inhibition of PKM2 was demonstrated in an in vivo HCT116 CRC model.

Conclusion

We show that the combination treatment showed superior anticancer efficacy as compared to 5-FU alone. These findings suggest that targeting PKM2 can increase the efficacy of chemotherapy, potentially providing a new approach for improving the outcome of chemotherapy in patients with CRC.

https://ift.tt/2BslvU2

Dose-dense paclitaxel/carboplatin as neo-adjuvant chemotherapy followed by radical surgery in locally advanced cervical cancer: a prospective phase II study

Abstract

Purpose

The role of dose-dense schedules in the neo-adjuvant treatment (NACT) of locally advanced cervical cancer (LACC) has been reported. This phase II study investigated activity of dose-dense paclitaxel/platinum before radical surgery (RS) in LACC patients.

Methods

The primary end-point was the rate of optimal pathological response (OPR: pathological complete/microscopic response). NACT (paclitaxel: 80 mg/m²) and carboplatin (AUC 2) were administered for 6 weeks. Overall response rate (ORR) to NACT was assessed by the RECIST criteria. Patients amenable to surgery were triaged to RS. The null hypothesis was that the OPR rate would improve from 30.0 to 45.0% (α error: 0.05, β error: 0.2). The regimen would be considered active if > 25 OPRs were found.

Results

36 patients were enrolled; 19 patients were stage IIB (52.8%) and 16 (44.4%) patients had pelvic lymph-node involvement at imaging. All patients completed neo-adjuvant chemotherapy; ORR was of 75.0%. RS was performed in 29 (93.5%) patients. Since the OPR was 16.1%, we evaluated the real chances to achieve the number of OPR required by the Simon design and decided to close the study. Grade 3/4 hematological toxicity occurred in 5 patients; surgical morbidity occurred in 14 patients. The 2-year PFS rate was 69.0%.

Conclusion

Dose-dense neo-adjuvant paclitaxel/carboplatin is feasible and safe in LACC patients; however, failure to achieve the primary end-point has to be recognized. Given the heterogeneity of the available studies, robust data from an adequately sized prospective study focused on more homogeneous series are required.

https://ift.tt/2RmmKtw

No significant impact of patient age and prior treatment profile with docetaxel on the efficacy of cabazitaxel in patient with castration-resistant prostate cancer

Abstract

Background

The correlation of the oncological outcomes of docetaxel and cabazitaxel in Japanese metastatic castration-resistant prostate cancer (mCRPC) patients has not been unclear.

Materials and methods

This study included a total of 47 consecutive Japanese mCRPC patients treated with cabazitaxel and assessed the prognostic significance of cabazitaxel, focusing on patient age and the correlation of efficacy between docetaxel and cabazitaxel.

Results

Prostate-specific antigen (PSA) decline was observed in 27 patients (57.4%), including 19 (40.0%) achieving the response defined by PSA decline ≥ 30%. The median overall survival (OS) periods after the introduction of cabazitaxel was 16.1 months. Twenty (42.6%) were judged to have responded to cabazitaxel with a PSA decrease ≥ 30% from the baseline. A 30% PSA response to cabazitaxel was achieved in 4 (50.0%) patients with ≧ 75 years (n = 8) and 16 (41.0%) patients with less than 75 years (n = 39). There was no significant correlation between the PSA response and patients' age (p = 0.707). A 30% PSA response to cabazitaxel was achieved in 13 (46.4%) and 7 (36.8%) patients with and without that to docetaxel, respectively. A 30% PSA response to cabazitaxel was achieved in 5 (16.6%) and 7 (41.2%) patients who had treated with less than 10 cycles docetaxel or 10 ≦ cycles, respectively. Univariate and multivariate analyses revealed that there were no significant correlation of patient age (p = 0.537), the response to prior docetaxel therapy (p = 0.339) or cycles of docetaxel therapy (p = 0.379) with shorter OS.

Conclusion

These results indicate that the introduction of cabazitaxel for Japanese mCRPC patients could result in oncological outcomes without any association with patient's age and the profiles of previous docetaxel therapy.

https://ift.tt/2QtJtDj

Thanks to YOU

We are Deeply Grateful to You!!! 

Thanks to YOU ECAN exceeded the $5,000
#GivingTuesday Fundraising Goal!

ECAN's generous supporters donated more than $6,000 – which was matched dollar-for-dollar by the
ECAN Board of Directors.

And because we exceeded our $5,000 goal, a generous individual has donated another $10,000 to our #GivingTuesday campaign!

That means your support resulted in more than $22,000 to fight Esophageal Cancer

 

Watch our Heartfelt Message of Thanks:

Because of YOU about $22,000 will now be available to Fight Esophageal Cancer by:

Raising Awareness,
Promoting Early Detection,
Supporting Medical Research and
Linking Patients and Families to Compassionate Support!!!

You Make a HUGE Difference!

The post Thanks to YOU appeared first on Esophageal Cancer Action Network.

https://ift.tt/2KTKi6P

Fever of Unknown Origin as a Sole Presentation of Subacute Thyroiditis in an Elderly Patient: A Case Report with Literature Review.

Related Articles

Fever of Unknown Origin as a Sole Presentation of Subacute Thyroiditis in an Elderly Patient: A Case Report with Literature Review.

Case Rep Endocrinol. 2018;2018:5041724

Authors: Raj R, Yada S, Jacob A, Unnikrishnan D, Ghali W

Abstract
An 80-year-old Caucasian male presented with fever of 3-week duration. Outpatient workup for infectious etiologies was negative and due to persistent fever, he was hospitalized for further evaluation of fever of unknown origin (FUO). Physical examination and laboratory studies remained unremarkable; however a follow-up CT scan of chest, abdomen, and pelvis with contrast done to rule out malignancy as an underlying cause of FUO revealed heterogeneous thyroid gland with surrounding hazy changes suggestive of thyroiditis. Thyroid function tests confirmed the diagnosis of subacute thyroiditis. The patient was started on prednisone with good response in his symptoms and was eventually discharged to home. The importance of our case lies in the fact that diagnosing subacute thyroiditis in the absence of classical symptoms of neck pain can be challenging and a physician should have a very high index of suspicion especially in an elderly patient where FUO can be the sole presentation.

PMID: 30498605 [PubMed]

https://ift.tt/2BQuEG4

Type 2 Diabetes Mellitus, a Sequel of Untreated Childhood Onset Growth Hormone Deficiency Developing in a 17-Year-Old Patient.

Related Articles

Type 2 Diabetes Mellitus, a Sequel of Untreated Childhood Onset Growth Hormone Deficiency Developing in a 17-Year-Old Patient.

Case Rep Endocrinol. 2018;2018:4748750

Authors: Henry RK, Menon RK

Abstract
In a seminal report, a 17-year-old boy with panhypopituitarism had fatty liver (FL) amelioration with growth hormone (GH). By extension, since hepatic insulin resistance (IR) is key to FL and type 2 diabetes mellitus (T2DM), GH then may ameliorate the IR of T2DM. We present a 17-year-old nonobese female with untreated childhood onset growth hormone deficiency (CO-GHD) who developed type 2 diabetes mellitus (T2DM) and steatohepatitis with bridging fibrosis. Based on height z-score of - 3.1 and a history of radiation therapy as treatment for a medulloblastoma at 7 years of age, GHD was quite likely. GH therapy was, however, not initiated at 15 years of age (when growth was concerning) based on full skeletal maturity. After she developed T2DM, GHD was confirmed and GH was initiated. With its initiation, though insulin dose decreased from 2.9 (~155 units) to 1.9 units/kg/day (~ 100 units), her T2DM was, however, not fully reversed. This illustrates the natural history of untreated CO-GHD and shows that though hepatic IR can be ameliorated by GH, full reversal of T2DM may be prevented with irreversible hepatic changes (fibrosis). Clinicians caring for pediatric patients and otherwise should remember that, even in patients beyond the cessation of linear growth, GH can have a crucial role in both glucose and lipid metabolism.

PMID: 30473891 [PubMed]

https://ift.tt/2KTKfb9

Marine-Lenhart Syndrome: Case Report, Diagnosis, and Management.

Related Articles

Marine-Lenhart Syndrome: Case Report, Diagnosis, and Management.

Case Rep Endocrinol. 2018;2018:3268010

Authors: Neuman D, Kuker R, Vendrame F

Abstract
The coexistence of thyroid functioning nodules and Graves' disease is called Marine-Lenhart syndrome. This condition is estimated to occur in 0.8-2.7% of patients with Graves' disease with few cases reported in the literature. Criteria for the diagnosis are not well defined. Here, we present a case of hyperthyroidism characterized by the presence of stimulating TSH receptor antibodies and severe bilateral exophthalmos. A thyroid uptake and scan revealed an elevated 24-hour iodine-131 uptake and a discrete hot nodule in the upper pole of the right lobe which was also observed with a thyroid ultrasound. The patient was diagnosed with Marine-Lenhart syndrome complicated by thyroid eye disease and was treated with methimazole followed by thyroidectomy and orbital decompression. We review the existing literature and propose criteria for the diagnosis and treatment of this condition.

PMID: 30473890 [PubMed]

https://ift.tt/2BQuzCg

Phase II Trial of Paclitaxel +/- Ramucirumab in Patients With Squamous-cell Carcinoma of the Esophagus After Prior Therapy

Condition:   Squamous Cell Carcinoma of the Esophagus
Interventions:   Drug: Ramucirumab;   Drug: Paclitaxel
Sponsors:   IKF Klinische Krebsforschung GmbH at Krankenhaus Nordwest;   Eli Lilly and Company;   Trium Analysis Online GmbH
Not yet recruiting

https://ift.tt/2KTKbbp

Second-Line Chemotherapy With Ramucirumab +/- Paclitaxel in Elderly Advanced Gastric or Gastroesophageal Junction Cancer Patients

Conditions:   Stomach Cancer;   Stomach Neoplasm;   Gastric Cancer;   Gastroesophageal Junction Adenocarcinoma
Interventions:   Drug: Ramucirumab;   Drug: Ramucirumab + Paclitaxel
Sponsors:   Federation Francophone de Cancerologie Digestive;   Eli Lilly and Company
Recruiting

https://ift.tt/2BR5l6Z

Tracheal Intubation Using Videolaryngoscope vs Fiberoptic Bronchoscope in Patients With Cervical Immobilization

Condition:   Intubation, Intratracheal
Intervention:   Procedure: intubation with videolaryngoscope vs fiberoptic bronchoscope
Sponsor:   Seoul National University Hospital
Not yet recruiting

https://ift.tt/2KTK4fZ

Palliative and Oncology Care Intervention: Symptom COACH

Condition:   Cancer of Head and Neck
Intervention:   Other: Evidenced-based symptom management and coping intervention
Sponsor:   Fox Chase Cancer Center
Recruiting

https://ift.tt/2BR25IK

Artificial Intelligence for Early Diagnosis of Esophageal Squamous Cell Carcinoma

Conditions:   Artificial Intelligence;   Optical Enhancement Endoscopy;   Magnifying Endoscopy
Interventions:   Other: AI presentation;   Other: no AI presentation
Sponsor:   Shandong University
Recruiting

https://ift.tt/2KVllYv

Swimming performance of a freshwater fish during exposure to high carbon dioxide

Abstract

Deterring the spread of invasive fishes is a challenge for managers, and bigheaded carp (including bighead and silver carp, Hypophthalmichthys spp.) are invasive fish that have spread throughout large portions of the Mississippi River basin and threaten to invade the Great Lakes' ecosystem. Studies have shown that elevated levels of carbon dioxide gas (CO₂) have the ability to act as a nonphysical fish barrier, but little work has been done on the efficacy of CO₂ to deter fish movement in flowing water. An annular swim flume was used to measure U_burst and sprint duration of the model species largemouth bass (Micropterus salmoides) across a range of pCO₂ levels (< 400 μatm [ambient]; 10,000 μatm; 50,000 μatm; and 100,000 μatm). This species was tested as a proxy because of the likelihood of a similar CO₂ response being produced, as well as constraints in obtaining and housing appropriately sized Asian carp. A significant decrease in U_burst swimming occurred when exposed to 100,000 μatm. No effects on sprint duration were detected. In both swimming tests, 15% of fish lost equilibrium when exposed to 50,000 μatm pCO₂, while 50% of fish lost equilibrium when exposed to 100,000 μatm. Together, results define target levels for managers to impede the spread of largemouth bass and potentially other invasive freshwater fishes, helping guide policy to conserve aquatic ecosystems.

https://ift.tt/2zFW1kZ

Amalgamation of N-graphene quantum dots with nanocubic like TiO 2 : an insight study of sunlight sensitive photocatalysis

Abstract

In this work, a sunlight-sensitive photocatalyst of nanocubic-like titanium dioxide (TiO₂) and N-doped graphene quantum dots (N-GQDs) is developed through a simple hydrothermal and physical mixing method. The successful amalgamation composite photocatalyst characteristics were comprehensively scrutinized through various physical and chemical analyses. A complete removal of bisphenol A (BPA) is attained by a synthesized composite after 30 min of sunlight irradiation as compared to pure TiO₂. This clearly proved the unique contribution of N-GQDs that enhanced the ability of light harvesting especially under visible light and near-infrared region. This superior characteristic enables it to maximize the absorbance in the entire solar spectrum. However, the increase of N-GQDs weight percentage has created massive oxygen vacancies that suppress the generation of active radicals. This resulted in a longer duration for a complete removal of BPA as compared to lower weight percentage of N-GQDs. Hence, this finding can offer a new insight in developing effective sunlight-sensitive photocatalysts for various complex organic pollutants degradation.

https://ift.tt/2PiKxsm

Glycemic variation in uncontrolled Graves’ disease patients with normal glucose metabolism: Assessment by continuous glucose monitoring

Abstract

Purpose

To observe the glycemic variation (GV) in uncontrolled Graves' disease (GD) patients with normal glucose metabolism measured by continuous glucose monitoring (CGM).

Methods

This was a single-center, open-label, observational study. From January 2017 to October 2017, 20 GD patients with normal glucose metabolism and 24 healthy control subjects were recruited. Serum samples were obtained at 0, 30, and 120 min after oral glucose loading for glucose, insulin, and C-peptide level measurements. Fasting plasma fasting free triiodothyronine (FT3), free thyroxin (FT4), and thyroid stimulating hormone concentrations were also detected. All participants were subjected to a 3-day CGM after baseline data were collected. The primary endpoint was the difference in the mean amplitude of the glycemic excursions between the two groups.

Results

Compared with the healthy subjects, the GD patients had higher mean amplitude of glycemic excursions (MAGE) (P < 0.01). Multiple linear stepwise regression analysis showed that FT4 level was an independent factor for the MAGE. Interestingly, the GD patients had a significant prolongation in the time to peak glucose, especially after breakfast (P < 0.01), and the elevation in the incremental area under the curve of glucose after breakfast till 4 hours later.

Conclusions

Uncontrolled GD patients with normal glucose metabolism had a greater GV, and the FT4 level may contributed to the increased GV.

https://ift.tt/2PiKXPM

Glycemic variation in uncontrolled Graves’ disease patients with normal glucose metabolism: Assessment by continuous glucose monitoring

Abstract

Purpose

To observe the glycemic variation (GV) in uncontrolled Graves' disease (GD) patients with normal glucose metabolism measured by continuous glucose monitoring (CGM).

Methods

This was a single-center, open-label, observational study. From January 2017 to October 2017, 20 GD patients with normal glucose metabolism and 24 healthy control subjects were recruited. Serum samples were obtained at 0, 30, and 120 min after oral glucose loading for glucose, insulin, and C-peptide level measurements. Fasting plasma fasting free triiodothyronine (FT3), free thyroxin (FT4), and thyroid stimulating hormone concentrations were also detected. All participants were subjected to a 3-day CGM after baseline data were collected. The primary endpoint was the difference in the mean amplitude of the glycemic excursions between the two groups.

Results

Compared with the healthy subjects, the GD patients had higher mean amplitude of glycemic excursions (MAGE) (P < 0.01). Multiple linear stepwise regression analysis showed that FT4 level was an independent factor for the MAGE. Interestingly, the GD patients had a significant prolongation in the time to peak glucose, especially after breakfast (P < 0.01), and the elevation in the incremental area under the curve of glucose after breakfast till 4 hours later.

Conclusions

Uncontrolled GD patients with normal glucose metabolism had a greater GV, and the FT4 level may contributed to the increased GV.

https://ift.tt/2PiKXPM

A Comparative Perspective on Extra-retinal Photoreception

Publication date: Available online 4 December 2018

Source: Trends in Endocrinology & Metabolism

Author(s): Jonathan H. Pérez, Elisabetta Tolla, Ian C. Dunn, Simone L. Meddle, Tyler J. Stevenson

Ubiquitous in non-mammalian vertebrates, extra-retinal photoreceptors (ERPs) have been linked to an array of physiological, metabolic, behavioral, and morphological changes. However, the mechanisms and functional roles of ERPs remain one of the enduring questions of modern biology. In this review article, we use a comparative framework to identify conserved roles and distributions of ERPs, highlighting knowledge gaps. We conclude that ERP research can be divided into two largely unconnected categories: (i) identification and localization of photoreceptors and (ii) linkage of non-retinal light reception to behavioral and physiological processes, particularly endocrine systems. However, the emergence of novel gene editing and silencing techniques is enabling the unification of ERP research by allowing the bridging of this divide.

https://ift.tt/2Ecr9MS

Serum sickness disease in a patient with alopecia areata and Meniere' disease after PRP procedure

Background

Platelet Rich Plasma procedure (PRP) is considered to be one of the safest aesthetic procedures. Adverse reactions after PRP administration are extreme rare.

Purpose

We present the patient with serum sickness disease (SSD) after PRP procedure.

Objective & Methods

41 years old female suffers from alopecia areata for 5 years with frequent relapses and she has been suffering from Menier's disease recurrent symptoms for 6 years. The patient developed SSD after third PRP rejuvenating procedure and she has also noticed new alopecia areata lessions, but without Menier's disease symptoms.

Results

After SSD, 4 months later, she developed severe symptoms of Menier's disease with an episode of sudden sensorineural hearing loss. It alleviated only after intravenous administration of methylprednisolone.

Conclusion

In our opinion, significant contraindication of PRP procedure is an autoimmune disease in the active phase.

This article is protected by copyright. All rights reserved.

https://ift.tt/2DYDcMJ

Tooth substance removal for ceramic single crown materials—an in vitro comparison

Abstract

Objectives

The aim of this in vitro study was to compare the tooth structure removal required for currently available ceramic crown materials.

Material and methods

Ninety typodont teeth (60 incisors, 30 molars) were assigned to nine study groups. The teeth were digitized, weighed with a high-precision balance, and fixed in carriers in identical alignment. Full-crown restorations were prepared according to material-specific guidelines for monolithic zirconia (MZ), polymer-infiltrated ceramics (PIC), buccally veneered zirconia (BVZ), feldspathic ceramics (FC), fully veneered zirconia (FVZ), and lithium disilicate (LD). Tooth structure removal was assessed by weighing the teeth before and after preparation. Coronal volume loss was analyzed statistically by use of one-way ANOVA and post-hoc Tukey HSD tests with α = 0.05.

Results

Mean tooth structure removal for incisors was 42% (SD 2%) for MZ, 46% (SD 1%) for PIC, 50% (SD 2%) for BVZ, 57% (SD 1%) for FC, 57% (SD 2%) for FVZ, and 59% (SD 2%) for LD. Mean tooth structure removal for molars was 21% (SD 2%) for MZ, 31% (SD 1%) for PIC, and 35% (SD 1%) for LD. Inter-group differences were statistically significant, except for between FC and FVZ.

Conclusions

Preparation of full ceramic crowns for restoration-free teeth is an invasive procedure. Selecting the ceramic material can, however, reduce loss of tooth structure substantially.

Clinical relevance

Monolithic zirconia is the least invasive material for the preparation of incisor and molar ceramic single crowns. Prescribing buccally veneered instead of fully veneered zirconia reduces preparation invasiveness significantly.

https://ift.tt/2QxJaux