Ιατρικά Άρθρα: 09/24/18

Δευτέρα 24 Σεπτεμβρίου 2018

Role of simvastatin in tumor lymphangiogenesis and lymph node metastasis

Abstract

Lymphangiogenesis plays a crucial role in promoting cancer metastasis to sentinel lymph nodes (LNs) and beyond. Increasing data have shown that simvastatin, a cholesterol-lowering medication for the prevention of cardiovascular diseases, is involved in tumor growth and dissemination, and endothelial functions. This study aimed to investigate the potential effect of simvastatin on lymphatic formation and LN metastasis. Tumor models were established by subcutaneous injection of B16-F10 melanoma cells into mouse hind footpads. Simvastatin was administered (0.2 µg/g, intraperitoneal injection, IP) every other day for a total of eight times. Tissue samples were removed and examined by immunohistochemical staining and reverse transcription-polymerase chain reaction (RT-PCR) techniques. The lymphatics of LN, skin, liver, and lung exhibited morphological changes, and LN weight and metastatic area of the tumor group treated with simvastatin was lower than that of the untreated tumor group. Analysis of lymphatic size, area fraction, and lymphatic vessel density showed tissue specificity and variation to melanoma carcinogenesis in the simvastatin-treated group compared with the untreated group. In addition, LNs and cutaneous tissues showed altered expression of lymphangiogenic factors and inflammatory cytokines such as VEGF-A/-C/-D and TNF-α. These findings indicated that simvastatin may modify lymphangiogenesis and tumor progression in malignant melanoma.

https://ift.tt/2OdR8YC

TEKT4 Promotes Papillary Thyroid Cancer Cell Proliferation, Colony Formation, and Metastasis through Activating PI3K/Akt Pathway

Abstract

Thyroid carcinoma is the most common malignancy of the endocrine system worldwide, but its molecular mechanisms remain unclear. Some diseases are associated with TEKT4 gene. However, its role in thyroid carcinoma has yet to be fully examined. This study was designed to investigate the function of TEKT4 in papillary thyroid cancer (PTC). The effect of TEKT4 on aggressive behavior of PTC cell lines, namely, TPC1 and BCPAP, transfected with small interfering RNA was identified through cell proliferation, colony formation, migration, and invasion. Our previous study revealed that TEKT4 may be vital in PTC. In in vitro experiments, TEKT4 downregulation suppressed cell proliferation, colony formation, cell migration, and invasion. Our data also indicated that tumor-suppressing role of TEKT4 knockdown in PTC cell lines was associated with the silence of the PI3K/Akt pathway. Our study revealed that TEKT4 shows important biological implications and is worthy of further study.

https://ift.tt/2O6hBHB

How to integrate physical activity and exercise approaches into inpatient treatment for eating disorders: fifteen years of clinical experience and research

Abstract

Background

The importance of physical activity and exercise among patients with eating disorders (EDs) is acknowledged among clinicians and researchers. The lack of clinical guidelines, the differing attitudes towards exercise approaches in treatment, and the lack of specialized competence all influence the management of ED symptoms in specialist ED treatment units. The purpose of the study was to examine 15 years of clinical experience with exercise approaches as an integrated part our inpatients treatment program.

Methods

From January 2003 to December 2017, 244 patients were admitted to a specialist ED unit in Norway. The treatment program at the ED unit is multidisciplinary. It is based on psychodynamic theory, and designed to enhance patients' recovery and to enable them to adopt a lifestyle that is as healthy as possible. The authors describe the clinical management of patients with reference to practical examples and a case example.

Recommendations and experience

The treatment for exercise is not manualized, but adjusted to the specific symptoms and needs of individual ED patients. The treatment approaches to exercise are part of the body-oriented treatment at the Specialist eating disorder unit (Regionalt kompetansesenter for spiseforstyrrelser (RKSF)), and the therapy addresses the entire body and the relation between the body, emotions, and the patient's social situation. It covers a chain of approaches from admission to discharge, from rest and relaxation to regular exercise groups.

Conclusions

Our experience and recommendations support earlier proposals for treatment approaches to exercise and exercise-related issues as a beneficial supplement to the treatment of ED inpatients. We have not experienced any adverse influences on patients' recovery processes, such as their rate of weight gain. Our intention is that this paper will be a contribution to the field of ED, the integration of exercise approaches in the inpatient treatment of ED and development of clinical guidelines.

https://ift.tt/2QViS2M

Short-term evaluation of photobiomodulation therapy on the proliferation and undifferentiated status of dental pulp stem cells

Abstract

The aim of this in vitro study was to analyze the effect of photobiomodulation therapy (PBMT) on the proliferation and undifferentiating status of stem cell from human exfoliated deciduous teeth (SHEDs). PBMT was carried out with an aluminum gallium indium phosphide (InGaAlP) diode laser in contact and punctual mode (continuous wave, 660 nm, 20 mW, 0.028 cm², and average energy densities of 1 (1 s), 3 (4 s), 5 (7 s), 10 (14 s), 15 (21 s), or 20 (28 s) J/cm² per point). The immunoprofile of the SHEDs was analyzed using flow cytometry. Cell proliferation was assessed by the MTT reduction assay. Gene expressions of mesenchymal stem cell markers (OCT4, Nestin, CD90, and CD105) were assessed by RT-qPCR 48 h after PBMT. Data were compared by analysis of variance (ANOVA) and Tukey's test (p ≤ 0.05). Cells cultured under nutritional deficit and treated with PBMT at 5 J/cm² presented similar cell growth than those of positive control group. Cell growth was significantly higher than those of other groups. Mesenchymal stem cell gene markers were still expressed after PBMT at 5 J/cm². In a short-term analysis, PBMT increases the number of stem cells with no interference in the undifferentiated state of the irradiated cells, which opens wide possibilities for application in tissue regeneration.

https://ift.tt/2xEV2jT

Sleep and the human impacts of climate change

In this issue of Sleep Medicine Reviews, Rifkin and colleagues (referred subsequently as RLP) [1] report on a systematic review of the literature – and present a conceptual framework for – the potential impacts of climate change on human sleep. Their review spans literature from 1980-2017 and results in 16 studies matching the authors' selection criteria. Across these studies, RLP report a tendency of exposure to warmer temperatures, extreme weather events, floods, or wildfires to associate with diminished total sleep times as well as incidences of sleep disruption.

https://ift.tt/2NC7ZVJ

Glucose intolerance in children with cystic fibrosis: a developing country’s perspective

Journal Name: Journal of Pediatric Endocrinology and Metabolism
Issue: Ahead of print

https://ift.tt/2NDAPVw

Scholar : Molecular Medicine Reports - Volume:18 Number:5

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TABLE OF CONTENTS

November-2018
Volume 18
Issue 5

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Roles of endoplasmic reticulum stress and autophagy on H2O2‑induced oxidative stress injury in HepG2 cells

Zhiming Wu, Huangen Wang, Sunyang Fang, Chaoyang Xu

Ετικέτες

Δευτέρα 24 Σεπτεμβρίου 2018

Abstract

Abstract

Abstract

Background

Methods

Recommendations and experience

Conclusions

Abstract

November-2018 Volume 18 Issue 5

Roles of endoplasmic reticulum stress and autophagy on H2O2‑induced oxidative stress injury in HepG2 cells

Anti‑breast cancer activity of selected 1,3,5‑triazines via modulation of EGFR‑TK

Bioinformatics analyses of significant genes, related pathways and candidate prognostic biomarkers in glioblastoma

Network analysis of membranous glomerulonephritis based on metabolomics data

Effect of chemokine CC ligand 2 (CCL2) on α‑synuclein‑induced microglia proliferation and neuronal apoptosis

Protective effects of α‑2‑macroglobulin on human bone marrow mesenchymal stem cells in radiation injury

Comprehensive analysis of multi Ewing sarcoma microarray datasets identifies several prognosis biomarkers

Long non‑coding RNA ferritin heavy polypeptide 1 pseudogene 3 controls glioma cell proliferation and apoptosis via regulation of the microRNA‑224‑5p/tumor protein D52 axis

Distribution and prognostic value of tumor‑infiltrating T cells in breast cancer

BACE1 gene silencing alleviates isoflurane anesthesia‑induced postoperative cognitive dysfunction in immature rats by activating the PI3K/Akt signaling pathway

Genetic mutation of familial dilated cardiomyopathy based on next‑generation semiconductor sequencing

Combination of pristimerin and paclitaxel additively induces autophagy in human breast cancer cells via ERK1/2 regulation

Pharmacological basis for application of scutellarin in Alzheimer's disease: Antioxidation and antiapoptosis

Elevated methylation of OPRM1 and OPRL1 genes in Alzheimer's disease

Effect of emodin on T cell subsets in NOD mice with NaI‑induced experimental autoimmune thyroiditis

Modular bioinformatics analysis demonstrates that a Toll‑like receptor signaling pathway is involved in the regulation of macrophage polarization

Cytoprotective effects of the medicinal herb Astragalus membranaceus on lipopolysaccharide‑exposed cells

Protein phosphatase 2A regulates the p38 signaling pathway to affect the migration of astrocytes

Deletion of poly(ADP‑ribose) polymerase-1 changes the composition of the microbiome in the gut

Amino acid starvation promotes podocyte autophagy through mammalian target of rapamycin inhibition and transcription factor EB activation

Angiotensin II upregulates cyclophilin A by enhancing ROS production in rat cardiomyocytes

Downregulation of FOXK2 is associated with poor prognosis in patients with gastric cancer

Molecular pathogenesis involved in human idiopathic pulmonary fibrosis based on an integrated microRNA‑mRNA interaction network

CXCL12 induces migration of oligodendrocyte precursor cells through the CXCR4‑activated MEK/ERK and PI3K/AKT pathways

Dicer affects cisplatin‑mediated apoptosis in epithelial ovarian cancer cells

Streptozotocin‑induced diabetic mice exhibit reduced experimental choroidal neovascularization but not corneal neovascularization

Regulation of the NLRP3 inflammasome and macrophage pyroptosis by the p38 MAPK signaling pathway in a mouse model of acute lung injury

Estrogen restricts the apoptosis of endometrial stromal cells by promoting TSLP secretion

Comparing the role of Ginkgolide B and Ginkgolide K on cultured astrocytes exposed to oxygen‑glucose deprivation

FKBP11 protects intestinal epithelial cells against inflammation‑induced apoptosis via the JNK‑caspase pathway in Crohn's disease

Two Paired Box 6 mutations identified in Chinese patients with classic congenital aniridia and cataract

Using Illumina Infinium HumanMethylation 450K BeadChip to explore genome‑wide DNA methylation profiles in a human hepatocellular carcinoma cell line

Adoptive transfer of xenoantigen‑stimulated T cell receptor Vβ‑restricted human regulatory T cells prevents porcine islet xenograft rejection in humanized mice

Protective effect of berberine on aconite‑induced myocardial injury and the associated mechanisms

Platelet‑rich fibrin exudate promotes the proliferation and osteogenic differentiation of human periodontal ligament cells in vitro

Role of the Rho/ROCK signaling pathway in the protective effects of fasudil against acute lung injury in septic rats

Identification of key genes and associated pathways in KIT/PDGFRA wild‑type gastrointestinal stromal tumors through bioinformatics analysis

Protective effects of taurine against inflammation, apoptosis, and oxidative stress in brain injury

Complement depletion with cobra venom factor alleviates acute hepatic injury induced by ischemia‑reperfusion

Aldehyde dehydrogenase activity in cryopreserved cord blood cells for quality assessment prior to transplantation

Theanine, an antitumor promoter, induces apoptosis of tumor cells via the mitochondrial pathway

Expression and clinical significance of ubiquitin‑specific‑processing protease 34 in diffuse large B‑cell lymphoma

Epigallocatechin‑3‑gallate protects against secondary osteoporosis in a mouse model via the Wnt/β‑catenin signaling pathway

miRNA‑328 overexpression confers cisplatin resistance in non‑small cell lung cancer via targeting of PTEN

Silencing of perilipin by short hairpin RNA inhibits proliferation and induces apoptosis in liposarcoma cells

Protection of insulin‑like growth factor 1 on experimental peripheral neuropathy in diabetic mice

Bafilomycin A1 alleviates depression‑like symptoms in chronic unpredictable mild stress rats

Huwe1 is a novel mediator of protection of neural progenitor L2.3 cells against oxygen‑glucose deprivation injury

Cantharidin inhibits melanoma cell proliferation via the miR‑21‑mediated PTEN pathway

Analysis of virulence diversity of 73 Helicobacter pylori strains isolated in Guizhou province, China

miR‑3120‑5p acts as a diagnostic biomarker in non‑small cell lung cancer and promotes cancer cell proliferation and invasion by targeting KLF4

Association between the methylation of six apoptosis‑associated genes with autism spectrum disorder

Inhibition of CCL19 benefits non‑alcoholic fatty liver disease by inhibiting TLR4/NF‑κB‑p65 signaling

Application of medical adhesive inhibits intimal hyperplasia involving the downregulation of ERK1/2 and eNOS levels

Post‑transplant lymphoproliferative disorder in hematopoietic stem cell transplant patients: A single center retrospective study between 2005 and 2012

Sevoflurane‑induced neurotoxicity is driven by OXR1 post‑transcriptional downregulation involving hsa‑miR‑302e

Attenuated endothelial function is associated with decreased endothelial progenitor cells and nitric oxide in premenopausal diabetic women

Ursolic acid alleviates inflammation and against diabetes‑induced nephropathy through TLR4‑mediated inflammatory pathway

SFRP5 serves a beneficial role in arterial aging by inhibiting the proliferation, migration and inflammation of smooth muscle cells

Immunization with lentiviral vector‑modified dendritic cells encoding ubiquitinated hepatitis B core antigen promotes Th1 differentiation and antiviral immunity by enhancing p38 MAPK and JNK activation in HBV transgenic mice

Molecular mechanisms regarding potassium bromate‑induced cardiac hypertrophy without apoptosis in H9c2 cells

Structure‑activity associations in novel farrerol derivatives with vasorelaxant properties

Sarcomatoid carcinoma of the pancreas: A case report and review of the literature

Long noncoding RNA LINC00978 promotes cell proliferation and invasion in non‑small cell lung cancer by inhibiting miR‑6754‑5p

Acute stimulatory effect of tumor necrosis factor on the basolateral 50 pS K channels in the thick ascending limb of the rat kidney

Silencing of pantothenate kinase 2 reduces endothelial cell angiogenesis

miR‑501‑3p sensitizes glioma cells to cisplatin by targeting MYCN

Celastrol inhibits glucocorticoid‑induced osteoporosis in rat via the PI3K/AKT and Wnt signaling pathways

LIMD2 targeted by miR‑34a promotes the proliferation and invasion of non‑small cell lung cancer cells

November-2018
Volume 18
Issue 5

23^nd World Congress on Advances in Oncology & 22^th International Symposium on Molecular Medicine

23 ^rd International Symposium on Molecular Medicine