Developing a Framework for Designing and Deploying Technology-Assisted Rehabilitation Post Stroke: A Qualitative Study

alexandrossfakianakis shared this article with you from Inoreader Developing a Framework for Designing and Deploying Technology-Assisted Rehabilitation Post Stroke: A Qualitative Study Via American Journal of Physical Medicine & Rehabilitation - Published Ahead-of-Print Objective Many unmet rehabilitation needs of patients with stroke can be addressed effectively using technology. However, technological solutions have not yet been seamlessly incorporated into clinical care. The purpose of this pilot study was to examine how to bridge the gaps between the recovery process, technology, and clinical practice to impact stroke rehabilitation meaningfully. Design Semi-structured interviews using a grounded theory approach with purposive sampling of 17 diverse expert providers in acute care, inpatient, and outpatient stroke rehabilitation settings. Common themes were identified from qualitative analyses of the transcribed conversations to develop a guiding framework from the emerging concepts. Results Four core themes emerged that addressed major barriers in stroke rehabilitation and technology-assisted solutions to overcome these barriers: 1) accessibility to quality rehabilitation, 2) adaptability to patient differences, 3) accountability or compliance with rehabilitation, and 4) engagement with rehabilitation. Conclusion The results suggest a four-pronged framework, the A3E framework which stands for Accessibility, Adaptability, Accountability and Engagement, to comprehensively address existing barriers in providing rehabilitation services. This framework can guide technology developers and clinicians in designing and deploying technology-assisted rehabilitation solutions for post-stroke rehabilitation, particularly using tele-rehabilitation. Corresponding author: Preeti Raghavan, MD, Address: 600 North Wolfe Street, Phipps Bldng. Suite 182, Baltimore, MD 21287. Phone number: 410-955-0703. Email: praghav3@jhmi.edu Acknowledgement of Funding: This work is supported in part by the National Science Foundation grants DRK-12 DRL: 1417769, ITEST DRL: 1614085, and RET Site EEC: 1542286; and NY Space Grant Consortium grant 76156-10488. Conflict(s)-of-Interest/Disclosure(s): All authors have completed the ICMJE uniform disclosure and declare no support from any organization for the submitted work; VK and PR have an issued patent on Game-based Sensorimotor Rehabilitator; PR reports consulting for Mirrored Motion Works, Inc., outside the submitted work. No other relationships or activities appear to have influenced the submitted work. Anna Palumbo is in training. Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Circular RNA circTP63 enhances estrogen receptor-positive breast cancer progression and malignant behaviors through the miR-873-3p/FOXM1 axis

alexandrossfakianakis shared this article with you from Inoreader Circular RNA circTP63 enhances estrogen receptor-positive breast cancer progression and malignant behaviors through the miR-873-3p/FOXM1 axis Via Anti-Cancer Drugs - Published Ahead-of-Print Circular RNAs (circRNAs) have been shown to play a functional role in a variety of cancers. However, few studies on circRNAs in estrogen receptor-positive breast cancer have been conducted. Here, we investigated the role of circRNA circTP63 in estrogen receptor-positive breast cancer progression and malignant behaviors. First, we observed increased expression of circTP63 in MCF7 cells relative to normal human mammary epithelial cell lines, such as DU4475 and MCF-10A, and the changed oncogenicity of MCF7 cells correlated with circTP63 overexpression and downregulation. Interestingly, a series of gain- and loss-of-function assays revealed that a higher level of FOXM1 was closely associated with MCF7 malignant behaviors induced by circTP63 overexpression. Further investigations showed that c ircTP63 sponged to miR-873-3p, which targeted FOXM1 mRNA and inhibited its expression. Mechanistically, circTP63 binds to miR-873-3p and prevents the targeting of FOXM1, thus inducing the progression and malignant behaviors of estrogen receptor-positive breast cancer, such as cell proliferation, cell cycle dysregulation, invasion, migration and even tumor growth. CircTP63 might be a potential biomarker or target to treat estrogen receptor-positive breast cancer patients in the future. Received 2 July 2020 Revised form accepted 25 September 2020 Supplemental Digital Content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal's website, www.anti-cancerdrugs.com. Correspondence to Yue-e Xu, Department of Thyroid and Breast Surgery, The Affiliated Huai'an Hospital of Xuzhou Medical University and The Second People's Hospital of Huai'an, 62 Huaihai Road(S), Huai'an 223002, China, Tel: +86 15195387009; e-mail: yueexu1206@163.com Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

S-allylcysteine induces cytotoxic effects in two human lung cancer cell lines via induction of oxidative damage, downregulation of Nrf2 and NF-κB, and apoptosis

alexandrossfakianakis shared this article with you from Inoreader S-allylcysteine induces cytotoxic effects in two human lung cancer cell lines via induction of oxidative damage, downregulation of Nrf2 and NF-κB, and apoptosis Via Anti-Cancer Drugs - Published Ahead-of-Print In this study, we investigated the putative cytotoxic effect elicited by the garlic-derived compound S-allylcysteine (SAC) in two human cancer cell lines (HCC827 and NCI-H1975) in order to develop an experimental approach to the therapeutic potential of this molecule for lung cancer. Cells were incubated for 24, 48 and 72 h in the presence of SAC (10 or 20 mM), which resulted in a concentration- and time-dependent decrease in cell viability and culture confluence in both cell lines. These effects were contrasted with – and validated through – those observed in an immortalized but nontumorigenic epithelial cell line from human bronchial epithelium (BEAS-2B, negative control) and an adenocarcinoma human alveolar basal epithelial cell line (A549, positive control). SAC (20� �mM at 72 h) also increased the oxidative damage to lipids, augmented apoptosis, and decreased the expression of the nuclear factor erythroid 2-related factor 2 (Nrf2) and the nuclear factor kappa B (NF-κB) proteins in HCC827 and NCI-H1975 cells. Our results establish the efficacy of SAC in reducing malignant growth and proliferation of lung tumor cells. This effect is mediated by the induction of oxidative damage associated with the downregulation of Nrf2 and NF-κB and their corresponding signaling pathways. Received 26 August 2020 Revised form accepted 4 October 2020 Correspondence to Ana Laura Colín-González, PhD, Banco de Tumores, Instituto Nacional de Cancerología, S.S.A., Av. San Fernando # 22, Tlalpan, CDMX 14080, México, Tel: +5255 5628 0400 (x18113); e-mail: laura_coling@yahoo.com.mx Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Cryptochrome 1 is modulated by blue light in human keratinocytes and exerts positive impact on human hair growth

alexandrossfakianakis shared this article with you from Inoreader Cryptochrome 1 is modulated by blue light in human keratinocytes and exerts positive impact on human hair growth Via Wiley: Experimental Dermatology: Table of Contents Abstract Photoactivation of cryptochrome‐family proteins by blue light is a well‐established reaction regulating physiology of plants, fungi, bacteria, insects and birds, while impact of blue light on cryptochrome synthesis and/or activity in human non‐visual cells remains unknown. Here, we show that 453 nm blue light induces cryptochrome 1 (CRY1) accumulation in human keratinocytes and the hair follicle. CRY1 is prominently expressed in the human anagen hair follicle, including epithelial stem cells. Specific silencing of CRY1 promotes catagen, while stimulation of CRY1 by KL001 prolongs anagen ex vivo by altering the expression of genes involved in apoptosis and proliferation. Together, our study identifies a role for CRY1 in sustaining human hair growth. Previously we demonstrated positive effects of 453 nm blue light on hair growth ex vivo. Taken all together, our study suggests that CRY1 might mediate blue light dependent positive effects on hair growth. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Overexpression of Fgf8 in the epidermis inhibits hair follicle development

alexandrossfakianakis shared this article with you from Inoreader Overexpression of Fgf8 in the epidermis inhibits hair follicle development Via Wiley: Experimental Dermatology: Table of Contents Abstract The hair follicle is a classical model for studying epithelial‐mesenchymal interactions. Given the critical role of fibroblast growth factor 8 (Fgf8) in embryonic development, we generated a mouse model that overexpresses Fgf8 specifically in the epidermis. Interestingly, these mutant mice exhibited stunted, smaller bodies and severe hypotrichosis. Histological analysis showed that the hair follicles in the mutants were arrested at stage 2 of hair development. The density of hair follicles in the mutant mice was also lower compared to that in the control mice. Overexpression of Fgf8 inhibited the proliferation of epidermal cells and simultaneously promoted apoptosis, leading to the arrest of hair follicle development. Further analysis showed that sonic hedgehog (Shh) and bone morphogenetic protein 4 (Bmp4) were downregulated and upregulated, respectively. To summarize, our study demonstrates that FGF signaling plays an important role in the regulation of hair folli cle development. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Paracrine roles of hormone receptors in Riehl’s melanosis: A quantitative analysis of estrogen and progesterone receptor expression patterns

alexandrossfakianakis shared this article with you from Inoreader Paracrine roles of hormone receptors in Riehl's melanosis: A quantitative analysis of estrogen and progesterone receptor expression patterns Via Wiley: Experimental Dermatology: Table of Contents Abstract The incidence of Riehl's melanosis (RM) is most common in the fifth or sixth decade of life with a female preponderance. As the skin is regarded a non‐reproductive organ on which sex steroid hormones act, a possible relationship between the pathogenesis of RM and sex steroid hormone receptors can be inferred. This study intended to evaluate the expression profile of estrogen receptor (ER)β and progesterone receptor (PR) in RM. Twelve lesional and perilesional normal‐appearing skin samples of RM patients and the skin of 12 healthy controls were retrieved for analysis. Real‐time PCR analysis and immunohistochemical studies were conducted for ER β and PR, respectively. The lesional and perilesional normal‐appearing skin of 12 patients with RM and the skin of 12 healthy controls were retrieved for analysis. Interestingly, the dermal ERβ immunostaining intensity was increased more in lesional skin than in perilesional skin. When compared to healthy controls, increased expr ession of ERβ and PR mRNAs was observed in the lesional skin of patients with RM. Of note, epidermal and dermal ERβ and dermal PR expressions showed increased staining intensities in the lesional skin of RM patients compared to healthy controls. The altered expression of ERβ and PR in RM supports the possible role of these hormone receptors in the pathogenesis of RM. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Far-Lateral Transcondylar Approach to a Right Cervicomedullary Dural Arteriovenous Fistula of the Posterior Fossa

alexandrossfakianakis shared this article with you from Inoreader Far-Lateral Transcondylar Approach to a Right Cervicomedullary Dural Arteriovenous Fistula of the Posterior Fossa Via Journal of Neurological Surgery Part B: Skull Base J Neurol Surg B Skull Base DOI: 10.1055/s-0040-1705162 Objectives Dural arteriovenous fistulas (DAVFs) at the cervicomedullary junction are uncommon and often accompanied by subarachnoid hemorrhage (SAH). We aim to illustrate in detail the microsurgical procedure for treating a DAVF located at the cervicomedullary junction. Design We present a two-dimensional operative video that includes clinical history, preoperative imaging, surgical strategy, still images with labels, clinical course, and postoperative imaging. Setting The microsurgery was performed at an academic medical center. Participant The patient is a 55-year-old female who presented with SAH, acute onset headache, nausea, and vomiting. Angiography demonstrated right vertebral artery vasospasm and a persistent arteriovenous shunt at the cervicomedullary junction supplied by small perforating arteries of the right vertebrobasilar junction (Fig. 1). Main Outcome Measures The patient was placed in the park-bench position with the head turned to the contralateral side. A hockey stick incision was made, followed by a right-side far-lateral transcondylar approach. Indocynanine green videoangiography was performed to help identify the areas of arteriovenous shunting. Multiple clips were placed to interrupt vessels that corresponded to arterial feeders at the level of the C1 and C2 nerve root sleeves (Fig. 2). The dura was closed in a water tight fashion and the posterior fossa was reconstructed with a titanium mesh. Results Postoperative imaging showed no evidence of continued arteriovenous shunting. The patient was discharged in good clinical condition with an uneventful postoperative course. Conclusion A deep understanding of the microsurgical vascular anatomy is necessary for successful occlusion of a cervicomedullary DAVF.The link to the video can be found at: https://youtu.be/-LfOcNB05BY. [...] Georg Thieme Verlag KG Rüdigerstraße 14, 70469 Stuttgart, Germany Article in Thieme eJournals: Table of contents  |  Abstract  |  open access Full text View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

A collaborative care psychosocial intervention to improve late life depression in socioeconomically deprived areas of Guarulhos, Brazil: the PROACTIVE cluster randomised controlled trial protocol

alexandrossfakianakis shared this article with you from Inoreader A collaborative care psychosocial intervention to improve late life depression in socioeconomically deprived areas of Guarulhos, Brazil: the PROACTIVE cluster randomised controlled trial protocol Via Trials - Latest Articles The elderly population has been growing in most low- and middle-income countries (LMIC), and depression is a common condition among these populations. The lack of integration between mental health and primary ... View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Effect of radiotherapy after breast-conserving surgery in elderly patients with early breast cancer according to the AJCC 8th Edition Breast Cancer Staging System in Japan.

alexandrossfakianakis shared this article with you from Inoreader Effect of radiotherapy after breast-conserving surgery in elderly patients with early breast cancer according to the AJCC 8th Edition Breast Cancer Staging System in Japan. Via Breast Cancer CONCLUSIONS: RT was associated with significantly improved RFS, but had no significant impact on OS in elderly patients with breast cancer after BCS. Adjuvant RT should be performed even for elderly patients with early breast cancer. PMID: 33141399 [PubMed - as supplied by publisher] (Source: Breast Cancer) View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Optimal, Large-Scale Propagation of Mouse Mammary Tumor Organoids

alexandrossfakianakis shared this article with you from Inoreader Optimal, Large-Scale Propagation of Mouse Mammary Tumor Organoids Via Journal of Mammary Gland Biology and Neoplasia AbstractTumor organoids mimic the architecture and heterogeneity of in vivo tumors and enable studies of collective interactions between tumor cells as well as with their surrounding microenvironment. Although tumor organoids hold significant promise as cancer models, they are also more costly and labor-intensive to cultivate than traditional 2D cell culture. We sought to identify critical factors regulating organoid growth ex vivo, and to use these observations to develop a more efficient organoid expansion method. Using time-lapse imaging of mouse mammary tumor organoids in 3D culture, we observed that outgrowth potential varies non-linearly with initial organoid size. Maximal outgrowth occurred in organoids with a starting size between ~10 to 1000 cells. Based on these observations, we ... View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Characterization of Organoid Cultures to Study the Effects of Pregnancy Hormones on the Epigenome and Transcriptional Output of Mammary Epithelial Cells

alexandrossfakianakis shared this article with you from Inoreader Characterization of Organoid Cultures to Study the Effects of Pregnancy Hormones on the Epigenome and Transcriptional Output of Mammary Epithelial Cells Via Journal of Mammary Gland Biology and Neoplasia AbstractThe use of mouse derived mammary organoids can provide a unique strategy to study mammary gland development across a normal life cycle, as well as offering insights into how malignancies form and progress. Substantial cellular and epigenomic changes are triggered in response to pregnancy hormones, a reaction that engages molecular and cellular changes that transform the mammary epithelial cells into "milk producing machines". Such epigenomic alterations remain stable in post-involution mammary epithelial cells and control the reactivation of gene transcription in response to re-exposure to pregnancy hormones. Thus, a system that tightly controls exposure to pregnancy hormones, epigenomic al terations, and activation of transcription will allow for a better understanding of such... View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Polycythämia vera in der differenzialdiagnostischen Abklärung kognitiver Defizite

alexandrossfakianakis shared this article with you from Inoreader Polycythämia vera in der differenzialdiagnostischen Abklärung kognitiver Defizite Via DMW - Deutsche Medizinische Wochenschrift Dtsch Med Wochenschr 2020; 145: 1625-1627 DOI: 10.1055/a-1219-9564 Anamnese Es erfolgt die Aufnahme einer 82-jährigen Patientin zur weiteren Abklärung zunehmender kognitiver Defizite und Gangunsicherheit. Diagnostik und Befunde In der durchgeführten Computertomografie des Kopfes zeigten sich die Zeichen einer fortgeschrittenen subkortikalen arteriosklerotischen Enzephalopathie (SAE). Laborchemisch fiel ein erhöhter Hämoglobinwert von 19,9 g/dl auf. Bei zusätzlicher Mutation in der JAK-2-Analyse wurde die Diagnose einer Polycythämia vera (Pv) gestellt. Nach Aderlass-Therapie zeigte sich eine Verbesserung der Kognition. Diskussion Die progredienten kognitiven Defizite sind bei vorbestehender SAE im Zusammenhang mit der neu diagnostizierten Pv zu sehen. Nach entsprechender Therapie der Pv ist auch langfristig eine Besserung der Kognition zu beobachten, sodass in diesem Fallbericht die Wichtigkeit einer Abklärung sekundärer, potenziell behandelbarer Ursachen von kognitiven Defiziten hervorgehoben wird. [...] Georg Thieme Verlag KG Rüdigerstraße 14, 70469 Stuttgart, Germany Article in Thieme eJournals: Table of contents  |  Abstract  |  Full text View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.