Recovery and utilization of collagen protein powder extracted from chromium leather scrap waste

Abstract

In this work, we investigate collagen protein powder (CPP) extracted from chromium leather scrap waste (CLSW). The composition and molecular weight distribution of CPP were determined by elemental analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), respectively. The microstructure and size distribution of CPP were then characterized by scanning electron microscopy (SEM) and nanometer analyzer instrument. Finally, CPP was treated with corn starch (CS), and the swelling behavior of the resulting CPP-CS blend was investigated in order to determine its range of applications. The experimental data showed that CPP contains 13 different amino-acids. CPP also displayed low mineral salt levels and a nitrogen content of 43.84%, indicating its potential use as an organic fertilizer. The molecular weight range of CPP is 6.5 to ~ 26.6 kDa. After the obtained CPP was blended with CS, the CPP-CS blend is endowed with optimal swelling properties and is able to overcome the solubility drawbacks of CPP alone. In addition, when the CPP was used as a natural fertilizer, the germination rate and height of kidney beans obviously increased.

http://bit.ly/2Tc4hAT

Arsenic, selenium, and metals in a commercial and vulnerable fish from southwestern Atlantic estuaries: distribution in water and tissues and public health risk assessment

Abstract

The anadromous catfish Genidens barbus is a vulnerable and economically important species from the Southwestern Atlantic Ocean. Concentrations of As, Co, Fe, Se, and Zn were determined in water and muscle, gill, and liver of catfish from two southwestern Atlantic estuaries (Brazil and Argentina) and health risk via fish consumption was evaluated. High spatial variability was observed in the metals, As, and Se distribution for both estuaries. Considering all tissues, element concentrations (mg/kg, wet weight) were As = 0.41–23.50, Co = 0.01–2.9, Fe = 2.08–773, Se = 0.15–10.7, and Zn = 3.97–2808). Most of the trace elements tended to be higher in Brazil than in Argentina, except for Co, Fe, Se, and Zn in liver and Fe and Co in muscle and gill, respectively. Arsenic accumulation order was muscle > liver ≥ gill. Only As (muscle) was above the maximum recommended by international guidelines at both estuaries. The target hazard quotient ranged from 0.10 to 1.58, suggesting that people may experience significant health risks through catfish consumption. Supposing that the inorganic/toxic As ranged between 1 and 20% of the total, the recommended maximum intakes per capita bases were 6.1–95 and 8.4–138 kg/year (wet weight) for Brazil and Argentina, respectively. Carcinogenic risk for As intake was within the acceptable range but close to the recommended limit (> 10⁻⁴). These results highlights the importance of quantifying the As species in catfish muscle in order to generate more reliable risk estimates.

http://bit.ly/2AX54P5

Vertical flow wetlands and hybrid systems for the treatment of landfill leachate

Abstract

Landfill leachates contain a variety of toxic compounds, which makes them one of the most difficult types of wastewater to be treated. An alternative "green" technology for leachate treatment is the use of constructed wetlands (CWs). The aims of this study were to select macrophytes and substrates to be used in vertical flow wetlands (VFWs) and to evaluate the performance of hybrid systems composed by a VFW and a horizontal subsurface flow (HSSW) or a free water surface flow (FWSW) wetlands for the treatment of a high ammonium concentration landfill leachate. In microcosms scale experiments, Typha domingensis, Scirpus californicus, and Iris pseudacorus were studied to assess their tolerance to raw and diluted leachate. Substrate selection for VFWs was evaluated using different layers of light expanded clay aggregate (LECA), coarse sand, fine sand, and gravel. Contaminant removals were higher in planted than in unplanted wetlands. Plants did not tolerate the raw effluent but showed a positive effect on plant growth when exposed to the diluted leachate. T. domingensis and I. pseudacorus showed higher contaminant removal ability and tolerance to landfill leachate than S. californicus. VFW with LECA + coarse sand showed the best performance in removal efficiencies. Hybrid system composed by VFW-FWSW planted with T. domingensis presented the best performance for the treatment of landfill leachate with high concentrations of ammonium.

http://bit.ly/2WoQGIy

Acute ecotoxicological effects of salicylic acid on the Polychaeta species Hediste diversicolor : evidences of low to moderate pro-oxidative effects

Abstract

Contamination of the aquatic environment by pharmaceutical drugs is an emerging issue in ecotoxicology. Aquatic organisms, in the presence of xenobiotics, tend to activate defensive mechanisms against toxic effects in order to mitigate and/or compensate for the toxic damages that frequently result from these interactions. Salicylic acid (SA) is a common drug, widely used in human medicine due to its analgesic, anti-inflammatory, and antipyretic properties, as well as its activity in terms of preventing platelet aggregation, among other clinical and cosmetic uses. It is commonly found in levels of the nanograms per liter to the micrograms per liter range in receiving waters, and its presence has been related to toxic effects in aquatic organisms, including oxidative stress. However, the number of studies that characterize the ecotoxicological profile of salicylates is still scarce and no studies have been published about the putative toxic effects of SA, especially in marine polychaetes. In order to determine the potential ecotoxicological effects caused by SA, individuals of the marine Polychaeta species Hediste diversicolor were exposed for 96 h to ecologically relevant concentrations of this compound, and several biochemical endpoints were evaluated, namely the activity of the antioxidant enzymes glutathione peroxidase (GPx) and catalase (CAT), the phase II biotransformation isoenzymes glutathione S-transferases (GSTs), the cholinergic enzyme acetylcholinesterase (AChE), and the determination of lipoperoxidative damage (thiobarbituric acid-reactive substances (TBARS) assay). The obtained results demonstrated that despite the pro-oxidative effects elicited by SA, exposure to realistic levels of this compound was not able to generate a state of oxidative stress, and the adaptive protective responses elicited by exposed individuals were effective enough to minimize and/or inhibit the damage potentially caused by overproduced reactive oxygen species.

http://bit.ly/2R9LzIk

Carcinoma of Unknown Primary with EML4‐ALK Fusion Response to ALK Inhibitors

AbstractWith the advent of next‐generation sequencing (NGS) and precision medicine, investigators have determined that tumors from different tissue sources that have the same types of genetic mutations will have a positive response to the same targeted therapy. This finding has prompted us to seek potential therapeutic targets for patients with carcinoma of unknown primary (CUP) using NGS technology. Here, we reported a case of a woman with CUP resistance to chemotherapy. We detected 450 cancer‐related gene alterations using three metastatic tumor specimens and found the presence of EML4 exon13 and ALK exon20 fusion. The tumor did respond to crizotinib, a first‐generation ALK inhibitor. When her tumor progressed, circulating tumor DNA detection revealed ALK L1196 M and G1269A mutation resistance to crizotinib, but she had a response to brigatinib. This case revealed that NGS technology used to detect the genetic alterations in patients with CUP might be a reliable method to find potential therapeutic targets, although the primary lesion could not always be confirmed.Key Points.This case exemplifies responsiveness to ALK inhibitor in carcinoma of unknown primary (CUP) with EML4‐ALK fusion.Next‐generation sequencing is an important diagnostic tool to find potential therapeutic targets in CUP.Liquid biopsy may be useful to provide critical information about resistance mechanisms in CUP to guide sequential treatment decision with targeted therapy.

http://bit.ly/2RdwYLN

Everolimus plus Exemestane for Hormone Receptor‐Positive Advanced Breast Cancer: A PAM50 Intrinsic Subtype Analysis of BOLERO‐2

AbstractBackground.The prognostic and predictive value of the two nonluminal (human epidermal growth factor receptor 2 [HER2]‐enriched and basal‐like) subtypes within advanced hormone receptor‐positive (HR+) breast cancer is currently unknown.Materials and Methods.This study retrospectively analyzed 261 tumors (80.7% primary; 19.3% metastatic) from the BOLERO‐2 study; BOLERO‐2 randomized 724 patients with advanced HR+/HER2‐negative breast cancer to everolimus plus exemestane or placebo plus exemestane. Tumors were classified using a PAM50 subtype predictor. Multivariable Cox regression analyses tested the independent prognostic significance of PAM50, and associations between PAM50 subtypes and treatment upon progression‐free survival (PFS) were evaluated.Results.Subtype distribution was 46.7% luminal A (n = 122), 21.5% HER2‐enriched (n = 56), 15.7% luminal B (n = 41), 14.2% normal‐like (n = 37), and 1.9% basal‐like (n = 5); HER2‐enriched subtypes were more common in metastatic versus primary tumors (32.0% vs. 18.7%; p = .038). Median PFS differences between luminal and nonluminal (6.7 vs. 5.2 months; adjusted hazard ratio, 0.66; 95% confidence interval [CI], 0.47–0.94; p = .020) and HER2‐enriched and non‐HER2‐enriched subtypes (5.2 vs. 6.2 months; adjusted hazard ratio, 1.53; 95% CI, 1.07–2.19; p = .019) were significant. Everolimus plus exemestane significantly improved median PFS versus placebo plus exemestane among patients with HER2‐enriched tumors (5.8 vs. 4.1 months; adjusted hazard ratio, 0.49; 95% CI, 0.26–0.90; p = .034); however, the association between HER2‐enriched tumors and everolimus benefit was nonsignificant (p = .433).Conclusion.The HER2‐enriched subtype was identified in a substantial proportion of advanced HR+/HER2‐negative breast tumors, and was a consistent biomarker of poor prognosis. Tailored therapies are therefore needed for HER2‐enriched tumors in the advanced HR+/HER2‐negative breast cancer setting.Implications for Practice.Using 261 tumor samples from the BOLERO‐2 phase III clinical trial, this study shows that a substantial proportion (20%–30%) of hormone receptor‐positive (HR+)/human epidermal growth factor receptor 2 (HER2)‐negative advanced breast cancers do not have a luminal A or B gene expression profile. This group of patients with nonluminal disease has a poor survival outcome regardless of the addition of everolimus to exemestane. This is the second study that confirms the prognostic value of this biomarker. Overall, these findings indicate a necessity to design novel clinical trials targeting nonluminal disease within HR+/HER2‐negative breast cancer.

http://bit.ly/2WiK7a7

Aromatase Inhibitors and Newly Developed Nonalcoholic Fatty Liver Disease in Postmenopausal Patients with Early Breast Cancer: A Propensity Score‐Matched Cohort Study

AbstractBackground.Unlike tamoxifen, the relationship between aromatase inhibitor use in postmenopausal patients with breast cancer and nonalcoholic fatty liver disease (NAFLD) has not been delineated.Materials and Methods.A retrospective analysis of 253 patients with early breast cancer without baseline NAFLD and treated with nonsteroidal aromatase inhibitors was performed. Among them, 220 patients were matched for sex, age, and menstruation status with healthy patients, and the prevalence of NAFLD was compared. NAFLD was determined by hepatic steatosis index in the absence of other known liver diseases. The presence of significant liver fibrosis in patients with NAFLD was determined noninvasively by AST‐platelet ratio index, FIB‐4 score, and NAFLD fibrosis score (NFS).Results.Postmenopausal patients with breast cancer undergoing treatment with aromatase inhibitors had higher prevalence of NAFLD independent of body mass index (BMI) and underlying diabetes mellitus (DM). Although the aromatase inhibitor group showed higher fibrotic burden by NFS, independent of BMI and DM, the proportion of advanced fibrosis did not show statistically significant differences between AI‐treated patients and the healthy patients. Those with abnormal baseline fasting glucose levels are suggested to have increased risk of NAFLD development after aromatase inhibitor treatment. In addition, patients with NAFLD developed after aromatase inhibitor use had significantly lower disease‐free survival than those without NAFLD, although there was no significant difference in overall survival.Conclusion.Results of this study suggest that inhibition of estrogen synthesis in postmenopausal women undergoing treatment with aromatase inhibitors could increase the risk of NAFLD, which might have some influence on the prognosis of patients with breast cancer.Implications for Practice.Unlike tamoxifen, the role of aromatase inhibitor treatment use in postmenopausal patients with breast cancer in development of fatty liver is not well known. In this propensity‐matched cohort study, postmenopausal patients with breast cancer treated with aromatase inhibitors had increased risk of nonalcoholic fatty liver disease compared with healthy women after menopause, independent of obesity and diabetes mellitus. The results show possible adverse influence of the newly developed fatty liver on breast cancer disease‐free survival and suggest a necessity for further validation. Fatty liver may need to be considered as an adverse event for aromatase inhibitor treatment.

http://bit.ly/2Rd8m5W

Phase II Study of the Triple Combination Chemotherapy of SOXIRI (S‐1/Oxaliplatin/Irinotecan) in Patients with Unresectable Pancreatic Ductal Adenocarcinoma

AbstractLessons Learned. The triple combination chemotherapy of SOXIRI (S‐1/oxaliplatin/irinotecan) in patients with unresectable pancreatic ductal adenocarcinoma was an effective treatment that appeared to be better tolerated than the widely used FOLFIRINOX regimen.SOXIRI regimen may provide an alternative approach for advanced pancreatic cancer.Background.In our previous phase I study, we determined the recommended dose of a biweekly S‐1, oxaliplatin, and irinotecan (SOXIRI) regimen in patients with unresectable pancreatic ductal adenocarcinoma (PDAC). This phase II study was conducted to assess the safety and clinical efficacy in patients with unresectable PDAC.Methods.Patients with previously untreated metastatic and locally advanced PDAC were enrolled. The primary endpoint was response rate (RR). Secondary endpoints were adverse events (AEs), progression‐free survival (PFS), and overall survival (OS). Patients received 80 mg/m2 of S‐1 twice a day for 2 weeks in alternate‐day administration, 150 mg/m2 of irinotecan on day 1, and 85 mg/m2 of oxaliplatin on day 1 of a 2‐week cycle.Results.Thirty‐five enrolled patients received a median of six (range: 2–15) treatment cycles. The RR was 22.8% (95% confidence interval [CI]: 10.4–40.1); median OS, 17.7 months (95% CI: 9.8–22.0); and median PFS, 7.4 months (95% CI: 4.2–8.4). Furthermore, the median OS in patients with distant metastasis was 10.1 months, whereas that in patients with locally advanced PDAC was 22.6 months. Major grade 3 or 4 toxicity included neutropenia (54%), anemia (17%), febrile neutropenia (11%), anorexia (9%), diarrhea (9%), and nausea (9%). There were no treatment‐related deaths.Conclusion.SOXIRI is considered a promising and well‐tolerated regimen in patients with unresectable PDAC.

http://bit.ly/2WdPB68

A Phase II Randomized Trial of Panitumumab, Erlotinib, and Gemcitabine Versus Erlotinib and Gemcitabine in Patients with Untreated, Metastatic Pancreatic Adenocarcinoma: North Central Cancer Treatment Group Trial N064B (Alliance)

AbstractLessons Learned. Dual epidermal growth factor receptor (EGFR)‐directed therapy with erlotinib and panitumumab in combination with gemcitabine was superior to gemcitabine and erlotinib, but the clinical relevance is uncertain given the limited role of gemcitabine monotherapy.A significantly longer overall survival was observed in patients receiving the dual EGFR‐directed therapy.The dual EGFR‐directed therapy resulted in increased toxicity.Background.Gemcitabine is active in patients with advanced pancreatic adenocarcinoma. The combination of erlotinib, an oral epidermal growth factor receptor (EGFR) inhibitor, and gemcitabine was shown to modestly prolong overall survival when compared with gemcitabine alone. The North Central Cancer Treatment Group (now part of Alliance for Clinical Trials in Oncology) trial N064B compared gemcitabine plus erlotinib versus gemcitabine plus combined EGFR inhibition with erlotinib and panitumumab.Methods.Eligible patients with metastatic adenocarcinoma of the pancreas were randomized to either gemcitabine 1,000 mg/m2 on days 1, 8, and 15 of a 28‐day cycle with erlotinib 100 mg p.o. daily (Arm A) or the same combination with the addition of panitumumab 4 mg/kg on days 1 and 15 of a 28‐day cycle (Arm B). The primary endpoint of the trial was overall survival. Secondary endpoints included progression‐free survival, the confirmed response rate, and toxicity. Comparison between arms for the primary endpoint was done with a one‐sided log‐rank test, and a p value less than .20 was considered statistically significant. Response rate comparison was done with Fisher's exact test. All other reported p values are two‐sided.Results.A total of 92 patients were randomized, 46 to each arm. The median overall survival was 4.2 months in Arm A and 8.3 months in Arm B (hazard ratio, 0.817; 95% confidence interval [CI], 0.530–1.260; p = .1792). The progression‐free survival was 2.0 months in Arm A and 3.6 months in Arm B (hazard ratio, 0.843; 95% CI, 0.555–1.280; p = .4190). A partial confirmed response was seen in 8.7% of patients on Arm A and 6.5% on Arm B (p = .9999). No patients had a complete response. Grade 3 and higher nonhematologic toxicities were more common in patients on Arm B compared with those on Arm A (82.6% vs. 52.2%; p = .0018).Conclusion.Dual EGFR‐directed therapy resulted in a significant prolongation of overall survival in patients with advanced adenocarcinoma of the pancreas but was associated with substantially increased toxicities. Dual EGFR‐directed therapy in combination with gemcitabine alone cannot be recommended for further study, as single‐agent gemcitabine is no longer considered an appropriate therapy for otherwise fit patients with metastatic pancreatic cancer.

http://bit.ly/2RdwOUH

Cutaneous involvement as the first manifestation of T‐lymphoblastic lymphoma and review of the literature.

Lymphoblastic lymphomas (LBL) are uncommon malignant neoplasms derived from immature T‐ or B‐ lymphoid progenitor cells. Although cutaneous involvement may reach 33% in B‐LBL, only twelve cutaneous cases of T‐ LBL have been published. We report the case of a 49‐year‐old woman with 2‐month history of erythematous‐violaceous plaques in the sternal region and breasts. Histopathologic examination showed a dense monomorphus infiltrate in dermis and positive immunostainings for CD3, CD99 and terminal deoxynucleotidyl transferase, thus indicating T‐LBL. Staging work‐up only revealed a mediastinal mass at diagnosis. After a 51‐month follow‐up and different treatment regimens, the patient remains alive although she has presented four relapses, all of them extramedullary.

This article is protected by copyright. All rights reserved.

http://bit.ly/2B5fZWT

Biomonitoring chronic lead exposure among battery manufacturing workers in Tunisia

Abstract

The aim of the study was the biomonitoring of the chronic exposure to Pb by measuring its levels in blood, urine, and hair of battery workers. Blood lead (BPb), urinary lead (UPb), hair lead (HPb), and urinary δ-aminolevulinic acid (UALA) levels were determined for 52 workers in a battery plant and compared to those of 20 non-occupational exposed subjects (controls). BPb and UPb levels were determined by graphite furnace atomic absorption spectrometry (GFAAS). HPb levels were measured by triple quadrupole ICP-MS and UALA levels were determined using cation exchanger column. The measured levels were significantly higher compared to the controls exceeding the OSHA cutoff values (p < 0.01). The GM mean levels of BPb, UPb, UALA, and HPb of workers were 715 μg L⁻¹, 331 μg L⁻¹, 16.3 mg g⁻¹, and 234 μg g⁻¹, respectively. The GM mean levels of BPb, UPb, UALA, and HPb of controls were 93.6 μg L⁻¹, 36.3 μg L⁻¹, 1.9 mg g⁻¹, and 1.8 μg g⁻¹, respectively. Significant correlations were observed between BPb and UALA (r = 0.630, p = 0.000), UPb and UALA (r = 0.566, p = 0.000), and between BPb and HPb (r = 0.466, p = 0.004). The significant correlation between BPb and HPb suggests the usefulness of hair for assessing occupational exposure particularly when the study area presents medium to high levels of Pb pollution. The association between Pb biomarkers and potential confounding factors revealed significant influence of the occupational factor over smoking and alcohol consumption. The results of this study urge for the reinforcement of the implemented engineering controls and safety measures in order to reduce exposure and to address the health issues related to Pb poisoning.

http://bit.ly/2sKCkV6

Bio-removal of cadmium from aqueous solutions by filamentous fungi: Trichoderma spp. and Piriformospora indica

Abstract

Six strains of Trichoderma spp. and Piriformospora indica have been studied for cadmium tolerance and bioaccumulation capacities by the poisoned food technique. A quantitative assay for Trichoderma spp. and P. indica was conducted in broth cultures supplemented with different cadmium concentrations (0–500 mg/l). In addition, the growth pattern of P. indica was determined by growing the fungus in a solid medium amended with eight concentrations of cadmium (0–200 mg/l). Generally, an increasing cadmium gradient in the culture medium suppressed the ability of fungi for cadmium accumulation. However, a negative relation was observed between the biomass production of fungi and cadmium uptake (q: mg/g biomass). Results showed that Trichoderma spp., especially T. simmonsii [UTFC 10063], are tolerant to cadmium toxicity and have a high ability to cadmium bioaccumulation. The biomass production of T. simmonsii [UTFC 10063] was significantly stimulated and increased by 46.1% when cadmium concentration increased from 0 to 125 mg/l. Moreover, 91.7 and 31.2% of cadmium removal was observed at 10 and 500 mg/l of the cadmium concentration, respectively. P. indica, however, showed a lower tolerance and removal efficiency for cadmium as compared with Trichoderma spp. Therefore, Trichoderma spp., especially T. simmonsii [UTFC 10063], can be exploited as potent bio-removal agents in cadmium-polluted aqueous solutions.

Graphical abstract

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http://bit.ly/2SbLEQs

Arginase Inhibition Improves Endothelial Function in an Age-Dependent Manner in Healthy Elderly Humans

Rejuvenation Research, Ahead of Print.


http://bit.ly/2RMjT1i

The Relationship Between Sleep Duration, Falls, and Muscle Mass: A Cohort Study in an Elderly Chinese Population

Rejuvenation Research, Ahead of Print.


http://bit.ly/2CKfaTy

Sono-advanced Fenton-like degradation of aromatic amines in textile dyeing sludge: efficiency and mechanisms

Abstract

In this paper, a novel strategy integrating ultrasound (US) with a Fenton-like (zero-valent iron/EDTA/air, ZEA) process was proposed for the removal of the refractory and carcinogenic aromatic amines (AAs) in textile dyeing sludge for the first time. The operating condition was optimized as 1.08 W/cm³ ultrasonic density, 15 g/L ZVI, and 1.0 mM EDTA, which could reach degradation efficiencies of 51.79% in US, 72.88% in ZEA, and 92.40% in US/ZEA system after 90-min reaction. Quenching experiments showed that electron transfer reactions generated by the iron ligands in ZEA brought about various reactive oxidative species (ROS), in which Fe (IV), O₂˙⁻, and ˙OH dominated the degradation. US induced sludge disintegration by ultrasonic shear, proven by particle size decrease and supernatant organic matter upsurge, which helps ROS contact with those pollutants in the sludge cavities. Besides, US facilitated the iron redox cycle for oxygen activation by promoting the corrosion of ZVI and stripping considerable ferric ions from sludge iron oxides which were verified by SEM, XRF, and XPS.

Graphical abstract

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http://bit.ly/2S0M3oH

Evaluation of osseous changes in dental panoramic radiography of thalassemia patients using mandibular indexes and fractal size analysis

Abstract

Objective

This study aimed to evaluate the radiomorphometric indexes and fractal dimension (FD) on dental panoramic radiography (DPR) of Thalassemia Major (TM) patients.

Methods

In 59 TM patients and in 59 healthy control subjects, mandibular cortical index (MCI), mandibular cortical width (MCW), panoramic mandibular index (PMI), and FD of four different regions (FD1-4) were evaluated and compared using DPRs.

Results

The distribution of MCI in TM patients was similar to control subjects (p > 0.05). The mean MCW was significantly lower in TM patients (p = 0.001). There was no significant difference in PMI between TM patients and control subjects. The mean of FD measured in the supracortical area above the angle of mandible (FD2), in the anterior to the mental foramen (FD4) and mean FD of four different regions was significantly lower in TM patients (p = 0.003, p = 0.001, and p = 0.014, respectively).

Conclusions

The radiomorphometric indices and fractal dimensions evaluated in the panoramic radiograph are capable of identifying thalassemia patients' jaw bone, and the results of DPR scanning can be used to refer these patients to appropriate medical investigation.

http://bit.ly/2HwbSbZ

Short-term exposure to tricyclic antidepressants delays righting time in marine and freshwater snails with evidence for low-dose stimulation of righting speed by imipramine

Abstract

Active pharmaceutical ingredients such as tricyclic antidepressants (TCAs) are contaminants of emerging concern which are commonly detected in wastewater effluent and which can disrupt the behavior of non-target organisms. In aquatic snails, the righting response is a critical behavior that has been shown to be inhibited by exposure to SSRI-type antidepressants. We exposed marine and freshwater snails to three tricyclic antidepressants (clomipramine, amitriptyline, and imipramine) for 1 h and measured righting response time. In the marine mud snail (Ilyanassa obsoleta), all three TCAs significantly increased righting time at concentrations as low as 156 μg/L. Similarly, in the freshwater snail Leptoxis carinata, all three TCAs increased righting time at concentrations as low as 263 μg/L. However, exposure to imipramine from 15.8 to 316 μg/L resulted in significantly faster righting time. Such low-dose stimulation and high-dose inhibition are characteristics of a hormetic response. We discuss the possible physiological mechanism of action of TCAs and other antidepressants on snail behavior, and the occurrence of non-monotonic, hormetic dose responses to human pharmaceuticals in the aquatic environment.

http://bit.ly/2CHlQ4U

Methodology Flaws and Implications of A Complementary Medicine Study

To the Editor In a retrospective case-control study using records from the US National Cancer Database with 1:4 matching of 258 vs 1032 patients with cancer, Johnson et al reported: (1) reduced survival among the 258 patients who used at least 1 conventional treatment together with complementary medicine (CM) compared with the 1032 patients who used only conventional treatment and (2) an association between the use of CM and refusal of standard treatment. The authors concluded that such refusal may explain the reduced survival. Herein, we briefly argue why we are not convinced that the authors' results support their conclusion.

http://bit.ly/2RKp3uA

Stripping the Fear of a Fatal Disease

This article describes the coping mechanism used by a patient with cancer.

http://bit.ly/2CGRFux

Rapidly Evolving Extensive Fluorodeoxyglucose-Positive Soft-Tissue Activity During Nivolumab Therapy

A 41-year-old woman with stage IIa melanoma of the upper thigh presented with diffuse erythematous induration of the upper thigh expanding to the lower left abdominal area and palpable subcutaneous nodules on the ipsilateral gluteal region and leg during nivolumab therapy. What is your diagnosis?

http://bit.ly/2RK1rX0

Association of Inherited Variants in CHEK2 With Susceptibility to Testicular Germ Cell Tumors

This case-control study evaluates the enrichment of germline pathogenic variants in the mendelian cancer predisposition DNA repair genes in patients with testicular germ cell tumors compared with healthy controls.

http://bit.ly/2CSCFtN

Patient and Caregiver Consent for Publication of Case Reports

This Viewpoint argues against policies mandating the patient consent process for publication and institutional review board approval of case studies.

http://bit.ly/2ROP0co

Efficacy and Safety of Avelumab for Patients With Recurrent or Refractory Ovarian Cancer

This phase 1b open-label study assesses the efficacy and safety of avelumab, an anti–programmed death ligand 1 agent, in a cohort of patients with previously treated recurrent or refractory ovarian cancer.

http://bit.ly/2RNTznl

Methodology Flaws and Implications of a Complementary Medicine Study

To the Editor The recently published study by Johnson et al evaluating the effect of complementary medicine (CM) use on survival in patients receiving curative cancer treatment is being misrepresented as evidence that CM may be harmful. There are 2 major methodological issues that challenge the validity of this study.

http://bit.ly/2CDHKpm

Methodology Flaws and Implications of a Complementary Medicine Study—In Reply

In Reply We appreciate the correspondence regarding our article and are happy for the opportunity to respond. The main critiques were that (1) we did not properly identify complementary medicine (CM), (2) we underascertained the number of patients who underwent CM, and (3) the definition of CM was not accurate.

http://bit.ly/2RLCwSZ

Methodology Flaws and Implications of a Complementary Medicine Study

To the Editor I congratulate Johnson and colleagues on their study in which they compared the overall survival rates, adherence to treatment, and characteristics of patients with cancer receiving conventional cancer treatment (CCT) with complementary medicine (CM) with those who did not receive CM. However, the authors did not clearly provide or refer to definitions of CM and alternative medicine. Indeed, they mentioned that patients who receive alternative medicine and CM behave similarly; however, there was no reference provided for this statement. According to the National Center for Complementary and Integrative Health, CM is used along with conventional medicine, whereas alternative medicine is used instead of CCT. Johnson and colleagues used the term CM throughout their article when they were referring to alternative medicine. There has been a long debate about CM, alternative medicine, and CCT in the health care system. Yet, CM has been monitored by the national health regulatory authorities in Europe and the United States. None of these health regulatory authorities have supported the use of alternative medicine, and they only refer to nonconventional treatments as CM. The lack of clarity between CM and alternative medicine in the article by Johnson and colleagues may cause confusion in the international health communities.

http://bit.ly/2CKfTns

Methodology Flaws and Implications of A Complementary Medicine Study

To the Editor From my perspective, the work by Johnson et al has 3 problems. Two are genuinely methodological, whereas the third is more related with the ethics of research. The 3 problems severely undermine the study's scientific rigor.

http://bit.ly/2RR7I3d

Radiotherapy-Associated Sarcoma After Breast Cancer Treatment

This population-based study describes the incidence, latency period, and outcomes of radiotherapy-associated angiosarcoma among patients with breast cancer from the Netherlands Cancer Registry.

http://bit.ly/2CJNrSY

Methodology Flaws and Implications of A Complementary Medicine Study

To the Editor In a recent article, Johnson and colleagues concluded that patients with curable cancers who use complementary medicine (CM) modalities are more likely than those not using CM modalities to refuse or delay conventional cancer treatment and subsequently bear a higher mortality burden. The purpose of this letter is 2-fold: to address a glaring methodological flaw in this study, and to address the ramifications this study may have for the practice of medicine.

http://bit.ly/2RLTbWA

The Distribution of Allied Dental Practitioners in Australia: Socio‐economics and rurality as a driver of better health service accessibility

Abstract

Objectives

Analysis of the distribution of allied dental practitioners relative to the socio‐economic profile of the population, to develop a baseline of employment demographics at a time of proposed deregulation of practicing restrictions.

Methods

A database of allied dental practitioners (ADP – oral health therapists, dental therapists and dental hygienists) was compiled from the public access register of the Australian Health Practitioner Regulation Agency. The principal practice locations limited to suburb and postcode were geocoded by latitude and longitude and superimposed on a map of the Australian landmass using QGIS software. Using Australian Bureau of Statistics data, the number of ADPs for each Statistical Area 2 (SA2) was counted and apportioned according to proportion of the population within each SA2 for each quintile of the Index of Relative Socio‐economic Disadvantage (IRSD). The results were reported according to oral health therapists, dental therapists and dental hygienists per 100 000 population for each IRSD quintile, by State and Territory, and the Australian Regional Index for Areas (ARIA+).

Results

The ADP to population ratio was not consistent for each professional class between states and territories, IRSD quintiles and (ARIA+) regional areas. Across Major Cities, South Australia was the State with the highest ratio of oral health therapists (10.6 ‐ 12.9) and dental hygienists (14.6 – 24.5) for all IRSD quintiles. Western Australia had the highest ratio of dental therapists (9.3 – 16.0).

Conclusion

There is no consistent pattern of distribution of ADPs relative to the socio‐economic profile of the population across states and territories, and regional (ARIA+) areas of Australia.

This article is protected by copyright. All rights reserved.

http://bit.ly/2RQDT2L

Joint effects of Si and mycorrhiza on the antioxidant metabolism of two pigeonpea genotypes under As (III) and (V) stress

Abstract

Arsenic (As) is the most hazardous soil contaminant, which inactivates metabolic enzymes and restrains plant growth. To withstand As stress conditions, use of some alleviative tools, such as arbuscular mycorrhizal (AM) fungi and silicon (Si), has gained importance. Therefore, the present study evaluated comparative and interactive effects of Si and arbuscular mycorrhiza-Rhizophagus irregularis on phytotoxicity of arsenate (As V) and arsenite (As III) on plant growth, ROS generation, and antioxidant defense responses in pigeonpea genotypes (Tolerant-Pusa 2002; Sensitive-Pusa 991). Roots of As III treated plants accumulated significantly higher total As than As V supplemented plants, more in Pusa 991 than Pusa 2002, which corresponded to proportionately decreased plant growth, root to biomass ratio, and oxidative burst. Although Si nutrition and AM inoculations improved plant growth by significantly reducing As uptake and the resultant oxidative burst, AM was relatively more efficient in upregulating enzymatic and non-enzymatic antioxidant defense responses as well as ascorbate–glutathione pathway when compared with Si. Pusa 2002 was more receptive to Si nourishment due to its ability to establish more efficient mycorrhizal symbiosis, which led to higher Si uptake and lower As concentrations. Moreover, +Si+AM bestowed better metalloid resistance by further reducing ROS and strengthening antioxidants. Results demonstrated that the genotype with more efficient AM symbiosis in As-contaminated soils could accrue higher benefits of Si fertilization in terms of metalloid tolerance in pigeonpea.

http://bit.ly/2CHYoo5

Deep sequencing reveals specific bacterial signatures in the subgingival microbiota of healthy subjects

Abstract

Objectives

This study aimed to define the comprehensive bacterial flora of the healthy oral cavity by identifying and comparing bacterial species in different subgingival sites using 454 sequencing of 16S rRNA genes.

Materials and methods

Subgingival plaque samples were taken from six target teeth (central incisor, first premolar, and first molar in both the maxilla and mandible) of 10 periodontally healthy patients. Bacterial DNA was extracted and next-generation sequencing of 16S rRNA genes was performed.

Results

Bacterial composition in phylum level was similar for all sites within the same individual irrespective of tooth location. Unweighted UniFrac distance values of microbiome also showed that average distance was significantly larger between subjects than between tooth locations of the same subjects.

Conclusions

The present results clarify the lack of effect of tooth location in the healthy subgingival microbiota. Results may suggest that any subgingival site can demonstrate similar subject-specific microbiota.

Clinical relevance

This investigation offers a better understanding of the uniqueness of the oral microbiome. The present study will facilitate sampling in future subgingival microbiological studies.

http://bit.ly/2DxfvdK

Design and testing of a 24-channel head coil for MR imaging at 3 T

Publication date: Available online 23 January 2019

Source: Magnetic Resonance Imaging

Author(s): Ye Li, Jo Lee, Lei Zhang, Qiaoyan Chen, Changjun Tie, Chao Luo, Xiaoliang Zhang, Dong Liang, Xin Liu, Hairong Zheng

Abstract

Purpose

To offer the new head coil option (with a lower channel count) of an RF coil array that provides comparable performance to the commercial 32-channel head coil.

Methods

The coil system comprised an upper shield (ten channels) and bottom section (fourteen channels). Signal-to-noise ratio (SNR) values for 2D unaccelerated imaging and the g-factor with an acceleration factor of 2–4 were evaluated in a phantom study. SNR performance and human brain and intracranial vessel wall imaging were conducted as part of the in vivo study. Both studies were conducted using the commercial head coil on the Siemens Tim Trio 3 T system for comparison.

Results

In the phantom study, the new head coil had an 8% higher SNR than the commercial head coil and demonstrated less than a 1% difference in the g-factor with the acceleration factor 2–4 in the right-to-left and anterior-to-posterior directions. In the in vivo study, the performance matched the results seen in the phantom study and showed high reproducibility in several human experiments. Structural images of the brain and intracranial vessel wall with an isotropic spatial resolution of 0.5 mm can be acquired with the new head coil.

Conclusion

This study demonstrates that the new head coil is capable of providing good SNR performance in both phantom and human experiments, can achieve similar acceleration ability compared to the commercial head coil, and is capable of obtaining brain and intracranial vessel wall images.

http://bit.ly/2RbPl3G

Anatomy Pathology

 Annals of Diagnostic Pathology

The frequency of <em>NOTCH1</em> variants in T-acute lymphoblastic leukemia/lymphoma and chronic lymphocytic leukemia/small lymphocytic lymphoma among Jordanian patients

Publication date: Available online 24 January 2019Source: Annals of Diagnostic PathologyAuthor(s): Nezeen Z. Abualhaj, Zain Dardas, Belal Azab, Dema Ali, Maher A. Sughayer, Tariq N. Aladily, Mamoun AhramAbstractThe transmembrane receptor NOTCH1 is thought to be associated with the development and progression of T-acute lymphoblastic leukemia (T-ALL)/T-lymphoblastic lymphoma (T-LBL) and chronic lymphocytic leukemia (CLL)/small lymphocytic lymphoma (SLL). The current study aimed to characterize NOTCH1...

Annals of Diagnostic Pathology

4h

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 Pathology International

Combined hepatocellular‐cholangiocarcinoma with angiosarcomatoid change: A case report with immunohistochemical study

Sarcomatoid combined hepatocellular‐cholangiocarcinoma (cHCC‐CCA) is a rare condition, with only 16 cases reported to date; however, there have been no reports of hepatic sarcomatoid carcinoma with angiosarcomatous features. Here, we report a rare case of cHCC‐CCA with angiosarcomatoid changes in a 77‐year‐old man. The tumor was biphasic with malignant epithelial and mesenchymal components. Histologically, the epithelial component was concordant with classical type cHCC‐CCA. The mesenchymal component...

Pathology International

1d

Intravascular large B‐cell lymphoma involving large blood vessels, three autopsy cases

Although intravascular large B‐cell lymphoma (IVLBCL) is an extranodal lymphoma characterized by the selective growth of lymphoma cells within the lumina of small vessels, we here report three autopsy cases of IVLBCL characterized by the proliferation within large blood vessels. These three cases were diagnosed as IVLBCL of the bone marrow or skin biopsy. Two cases died suddenly before treatment, whereas the other died during treatment. Autopsies showed a large embolus of dense lymphoma cells extending...

Pathology International

1d

Mark above section as read

 Cells Tissues Organs

Whey Protein Complexes with Green Tea Polyphenols: Antimicrobial, Osteoblast-Stimulatory, and Antioxidant Activities

Polyphenols are known for their antimicrobial activity, whilst both polyphenols and the globular protein β-lactoglobulin (bLG) are suggested to have antioxidant properties and promote cell proliferation. These are potentially useful properties for a tissue-engineered construct, though it is unknown if they are retained when both compounds are used in combination. In this study, a range of different microbes and an osteoblast-like cell line (human fetal osteoblast, hFOB) were used to assess the combined...

Cells Tissues Organs

2h

Two Web Resources Linking Major Human Embryology Collections Worldwide

Human embryology is a core subject for medicine and current research. While animal models of development now have significant online resources available, the vast majority of human embryonic material is locked up in historic collections. When accessed today, these collections are still contributing to our understanding of human development. This paper describes two online resources for studying human development that are unlocking these invaluable collections and providing related human developmental...

Cells Tissues Organs

1d

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 Case Reports in Pathology

A Rare Case of Fibrous Hamartoma of Infancy: A Clinicopathological Diagnosis at a Tertiary Hospital, Eastern Nepal

Background. Fibrous hamartoma of infancy is a rare soft tissue lesion of infants and young children with characteristic triphasic morphology. Case Description. An 18-month-old female child was presented with complaints of swelling over right leg shin since birth. On examination, a lump of size 7x3 cm was identified which was mobile and nontender. Local excision was performed and tissue sent for histopathological examination. On gross examination, a globular, capsulated, firm to hard tissue had cut...

Case Reports in Pathology

1d

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 Cancer Cytopathology

The prevalence and surgical outcomes of Hurthle cell lesions in FNAs of the thyroid: A multi‐institutional study in 6 Asian countries

Background Hurthle cell‐rich nodules (HCNs) encompass non‐neoplastic to malignant lesions. There is paucity of literature on the frequency distribution of HCNs among Bethesda categories, histologic follow‐up, risk of malignancy (ROM), and risk of neoplasia (RON). The objective of this retrospective, multi‐institutional study was to determine the prevalence of the cytologic diagnostic category and surgical outcomes of patients with HCN. Methods Nine tertiary health centers representing 6 Asian...

Cancer Cytopathology

1d

Negative predictive value and sensitivity of urine cytology prior to implementation of The Paris System for Reporting Urinary Cytology

Background Urinary tract cytology (UTCy) is used for screening urothelial carcinoma (UC) and it must have a high negative predictive value (NPV) to be an effective test. To the authors' knowledge, the literature regarding the NPV of UTCy provides little information regarding the risk of malignancy, especially for patients with high‐grade urothelial carcinoma (HGUC). Methods Patients with negative UTCy specimens were identified in the pathology files at the study institution for the years 2012...

Cancer Cytopathology

1d

The value of cytology in the management of patients with pancreatic cysts

Cancer Cytopathology

1d

Comprehensive evaluation of ecological compensation effect in the Xiaohong River Basin, China

Abstract

Due to the complexity of ecological compensation standards and methods, some problems cannot be expected before compensation, but the evaluation of eco-compensation effect can feedback the implementation effect of eco-compensation policies and provide reference for the improvement of eco-compensation policies. This paper constructed a comprehensive evaluation index system of eco-compensation, which covered social and economic development, pollution discharge and monitoring, and pollution treatment aspects. An eco-compensation comprehensive evaluation model was established, which consisted of the projection pursuit model (PP model) and the chaotic particle swarm optimization algorithm (CPSO algorithm). The Xiaohong River Basin in China was selected as a case study. Before and after the implementation of eco-compensation policy, the compensation effects in the four counties in the basin from 2008 to 2015 were evaluated. The results showed that through the implementation of the basin eco-compensation policies, the comprehensive indicators in the four counties showed an upward trend, which indicated that the eco-compensation of the basin had achieved certain effects. Among them, Xincai was the best, and could provide reference for other counties. The research results can provide new ideas and new methods for the evaluation of eco-compensation effects.

http://bit.ly/2FILmL3

OroFacial Medicine

Prevalence of oral mucosal disorders during pregnancy: A systematic review and meta‐analysis

Abstract Objective To assess the prevalence of oral mucosal disorders during pregnancy. Methods Observational studies were selected by two reviewers in a two‐phase process. Search strategies were applied at CINAHL, LILACS, LIVIVO, PubMed, Scopus, Web of Science, Google Scholar, Open Grey, and ProQuest. The risk of bias was assessed using the Joanna Briggs Institute's Critical Appraisal Checklist for Studies Reporting Prevalence Data. Synthesis of results was calculated by the software R...

Oral Pathology & Medicine

4h

Skeletal stability in orthognathic surgery with the surgery first approach: a systematic review

Publication date: Available online 23 January 2019Source: International Journal of Oral and Maxillofacial SurgeryAuthor(s): D. Soverina, G. Gasparini, S. Pelo, P. Doneddu, M. Todaro, R. Boniello, C. Azzuni, C. Grippaudo, G. Saponaro, G. D'Amato, U. Garagiola, A. MoroAbstractThe surgery first approach (SFA) is a therapeutic strategy used in orthognathic surgery that is constantly evolving. With this approach, the pre-surgical orthodontic treatment can be eliminated, the maxilla and the mandible are...

Oral and Maxillofacial Surgery

5h

Pharyngeal airway space changes after maxillomandibular advancement: a five-year retrospective study

Publication date: Available online 23 January 2019Source: International Journal of Oral and Maxillofacial SurgeryAuthor(s): P.M. Araújo, R.L.V. Osterne, A.C.G. de Souza Carvalho, N.O. Azevedo, R.F. Gondim, R.T. Gonçalves Filho, E. Sant'Ana, R.L.M. NogueiraAbstractThe aim of this study was to compare the alterations in three regions of the airway—nasopharynx, oropharynx, and hypopharynx—in relation to the area of the midsagittal plane, volume, and minimal axial area after maxillomandibular advancement...

Oral and Maxillofacial Surgery

5h

Assessment of metal sleeve-free 3D-printed implant surgical guides

Publication date: Available online 23 January 2019Source: Dental MaterialsAuthor(s): Kyung Chul Oh, Ji-Man Park, June-Sung Shim, Jee-Hwan Kim, Jong-Eun Kim, Jang-Hyun KimAbstractObjectivesThe aim of the present study was to investigate the adaptation and guide hole tolerance of metal sleeve-free computer-assisted implant surgical guides fabricated with 3D printers.MethodsAn implant surgical guide for full-guided implant placement was designed with a total of eight different guide holes. Ten implant...

Dental Materials

5h

Microvascular reconstruction and dental rehabilitation of benign severely atrophic jaws and defects of the alveolar ridge: our philosophy in 2019

We deal regularly with patients who present with severe atrophy of the jaws, compromised soft tissue, or penetrating defects of the alveolar ridge that are not the result of malignant disease. For these patients we use microvascular bony flaps together with dental implants and implant-supported prostheses. The purpose of this retrospective study was to present our current management and the lessons we have learned over a 16-year period while treating 86 patients for these indications with 87 microvascular...

Oral and Maxillofacial Surgery

15h

Pharyngeal airway space changes after maxillomandibular advancement: a five-year retrospective study

The aim of this study was to compare the alterations in three regions of the airway—nasopharynx, oropharynx, and hypopharynx—in relation to the area of the midsagittal plane, volume, and minimal axial area after maxillomandibular advancement (MMA) surgery. Thirty patients who had undergone MMA surgery were evaluated at four time points: preoperative (T0), immediately postoperative (T1), 1year postoperative (T2), and ≥5 years postoperative (T3). All measurements were performed using computed tomography,...

Oral & Maxillofacial Surgery

16h

Skeletal stability in orthognathic surgery with the surgery first approach: a systematic review

The surgery first approach (SFA) is a therapeutic strategy used in orthognathic surgery that is constantly evolving. With this approach, the pre-surgical orthodontic treatment can be eliminated, the maxilla and the mandible are surgically repositioned into the desired position, and the therapy is ended with a short orthodontic phase. Several studies have reported that the SFA is an acceptable approach, but postoperative stability is unclear. In this study, a systematic review on the SFA was performed.

Oral & Maxillofacial Surgery

16h

Predictive value of FHIT, p27, and pERK1/ERK2 in salivary gland carcinomas: a retrospective study

Abstract Objectives The aim of this study was to investigate the predictive value of the biomarkers FHIT, p27, and pERK1/ERK2 in salivary gland carcinomas. Material and methods Immunohistochemical staining of FHIT, p27, and pERK1/ERK2 of 265 patients with salivary gland carcinomas was conducted, and associations with clinico-histopathological data, overall...

Clinical Oral Investigations

18h

Fludroxycortide cream as an alternative therapy for actinic cheilitis

Abstract Objectives The objective of the study is to assess, by clinical follow-up, the efficacy of the dermatological cream Fludroxycortide 0.125 mg/g (Drenison®) in the treatment of actinic cheilitis (AC). Material and methods Twenty-three patients diagnosed with AC participated in the study. Fifteen were submitted to corticotherapy with Fludroxycortide,...

Clinical Oral Investigations

18h

Systemic comorbidities are associated with medication‐related osteonecrosis of the jaws: case‐control study

Abstract Objectives Medication‐related osteonecrosis of the jaws (MRONJ) is a serious condition developed in up to 15% of patients who take antiresorptive medications. Its underlying pathogenesis remains unclear. The association between systemic comorbidities and MRONJ was investigated. Subjects and Methods A case‐control study was conducted in Brisbane, Australia. Hospital records were used to identify 68 cases of MRONJ between January 2003‐ March 2017. Each case was individually matched...

Oral Diseases

20h

The role of autophagy in the pathogenesis of periodontal disease

Abstract Periodontal disease is a chronic inflammatory disease leading to destruction of periodontal tissues. As a local inflammation, periodontopathic bacterium, pro‐inflammatory mediators and local immune response play pivotal role in the progress of periodontal disease. Besides, cigarette smoke has long been associated with periodontal disease and tooth loss. Autophagy is an intracellular degradation process highly conserved from yeast to humans. As a lysosomal degradation pathway of self‐digestion,...

Oral Diseases

20h

Following in the footsteps of Hippocrates—interrelation between the incidence of odontogenic abscess and meteorological parameters

Abstract Objectives Although many physicians in daily practice assume a connection between odontogenic infections and meteorological parameters, this has not yet been scientifically proven. Therefore, the aim of the present study was to evaluate the incidence of odontogenic abscess (OA) in relation to outdoor temperature and atmospheric pressure. Patients and methods ...

Clinical Oral Investigations

20h

Scanning electron microscopy analysis of aligner fitting on anchorage attachments

Abstract Purpose The aims of the study were (1) to evaluate the fitting of three different aligners (Invisalign [Align Technology, Santa Clara, CA, USA], CA Clear Aligner [Scheu-Dental, Iserlohn, Germany] and F22 [Sweden&Martina, Due Carrare, Italy]) on anchorage attachments using scanning electron microscopy (SEM), and (2) to analyze the influence of 2 different types of resin used to build attachments on aligner fitting. ...

Latest Results for Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie

22h

Impact of breastfeeding and/or bottle-feeding on surgical wound dehiscence after cleft lip repair in infants: a systematic review

Publication date: Available online 23 January 2019Source: Journal of Cranio-Maxillofacial SurgeryAuthor(s): Eriko Matsunaka, Shingo Ueki, Kiyoko MakimotoSummaryBackgroundImmediately after cleft lip repair, breastfeeding and bottle-feeding are generally restricted to avoid placing tension on the surgical incision. However, no consensus about feeding methods after cleft lip repair has been reached. The objective of this systematic review was to examine the impact of breastfeeding and/or bottle-feeding...

Cranio-Maxillofacial Surgery

1d

An <em>in vitro</em> long-term study of cryopreserved umbilical cord blood-derived platelet-rich plasma containing growth factors—PDGF-BB, TGF-β, and VEGF

Publication date: Available online 22 January 2019Source: Journal of Cranio-Maxillofacial SurgeryAuthor(s): Kyoko Baba, Yasuharu Yamazaki, Yumiko Sone, Yoshika Sugimoto, Kazuno Moriyama, Takayuki Sugimoto, Kennichi Kumazawa, Yasuhito Shimakura, Akira TakedaSummaryPurposeUmbilical cord blood-derived platelet-rich plasma (UCB-PRP) containing growth factors has attracted attention as a biomaterial useful for regenerative medicine. The osteoblastic differentiation of umbilical cord-derived mesenchymal...

Cranio-Maxillofacial Surgery

1d

Analysis of the association of <em>IL4</em> polymorphisms with orthodontic mini-implant loss

Publication date: Available online 23 January 2019Source: International Journal of Oral and Maxillofacial SurgeryAuthor(s): T.F. Lopes, C.M. Souza, A.M. Reichow, A.C. Melo, P.C. TrevilattoAbstractThe aim of this study was to investigate the association of clinical characteristics and IL4 tag single nucleotide polymorphisms (SNPs; rs2227284 and rs2243268) with orthodontic mini-implant (MI) failure. The sample included 135 subjects of both sexes, mean age 48.7 ± 10 years (range 20–76 years): 104 in...

Oral and Maxillofacial Surgery

1d

Implant fracture failure rate and potential associated risk indicators: An up‐to 12‐year retrospective study of implants in 5124 patients

Abstract Objectives This study investigated fracture rates and risk indicators for fractures in internal connection dental implants. Material and Methods We performed a retrospective analysis of 19,006 internal connection implants used in fixed restoration in 5,124 patients (4,570 males, 554 females) at the Dental Hospital of Veterans Health Service Medical Center between 2006 and 2015. Patients were followed through June 2018 (0.03–12.39 years post‐installation). Clinical factors (age,...

Clinical Oral Implants Research

1d

Assessment of Interleukin – 18 gene polymorphism and serum levels in Oral Lichen Planus in an Indian population

Abstract Background Oral Lichen Planus (OLP) is a chronic, inflammatory disease with uncertain aetiology. The aim of this study was to assess Interleukin‐18 (IL‐18) gene polymorphism and serum levels in OLP cases of Indian origin and to compare them with a control population of similar background. Methods Assessment of single nucleotide polymorphisms (SNPs) of IL‐18 gene at promoter regions 137(G/C) and 607(C/A) was done in 70 OLP cases and 70 healthy controls using sequence specific primer‐polymerase...

Oral Pathology & Medicine

1d

N-acetyl cysteine versus chlorhexidine mouthwashes in prevention and treatment of experimental gingivitis: a randomized, triple-blind, placebo-controlled clinical trial

Abstract Objectives To compare the efficacy of N-acetyl cysteine (NAC) mouthwash with chlorhexidine (CHX) in prevention and treatment of experimental gingivitis Materials and methods Sixty subjects were assigned randomly and blindly into one of three equal groups: NAC, CHX, or placebo group. The study was conducted in two stages: preventive and treatment...

Clinical Oral Investigations

1d

Investigation into the accuracy and measurement methods of sequential 3D dental scan alignment

Publication date: Available online 23 January 2019Source: Dental MaterialsAuthor(s): Saoirse O'Toole, Cecilie Osnes, David Bartlett, Andrew KeelingAbstractObjectivesAlignment procedures have yet to be standardised and may influence the measurement outcome. This investigation assessed the accuracy of commonly used alignment techniques and their impact on measurement metrics.MethodsDatasets of 10 natural molar teeth were created with a structured-light model-scanner (Rexcan DS2, Europac 3D, Crewe)....

Dental Materials

1d

A novel histopathological scoring system for patients with oral squamous cell carcinoma

Abstract Objectives Tumor invasion into blood and/or lymphatic vessels, perineural invasion, and histopathological grading are evaluated to assess the biological aggressiveness of oral squamous cell carcinoma (OSCC). We aim to assess the prognostic impact of a novel scoring system, based upon the aforementioned histological parameters. Materials and methods ...

Clinical Oral Investigations

1d

Analysis of the association of IL4 polymorphisms with orthodontic mini-implant loss

The aim of this study was to investigate the association of clinical characteristics and IL4 tag single nucleotide polymorphisms (SNPs; rs2227284 and rs2243268) with orthodontic mini-implant (MI) failure. The sample included 135 subjects of both sexes, mean age 48.7±10years (range 20–76years): 104 in the control group (patients without any MI loss) and 31 in the study group (patients presenting ≥1 MI loss). Genotypes were determined by real-time PCR. Bivariate and multivariate analyses were performed...

Oral & Maxillofacial Surgery

1d

Influence of Porphyromonas gingivalis in gut microbiota of streptozotocin‐induced diabetic mice

Abstract Objectives Increasing evidence suggests that periodontitis can exacerbate diabetes, and gut bacterial dysbiosis appears to be linked with the diabetic condition. The present study examined the effects of oral administration of the periodontopathic bacterium, Porphyromonas gingivalis, on the gut microbiota and systemic conditions in streptozotocin‐induced diabetic mice. Materials and Methods Diabetes was induced by streptozotocin injection in C57BL/6J male mice (STZ). STZ and wild‐type...

Oral Diseases

1d

Overexpression of EphrinB2 in Stem Cells from Apical Papilla Accelerates Angiogenesis

Abstracts Objectives we aimed to accelerate angiogenesis in pulp regeneration by modulating ephrinB2 expression in stem cells from apical papilla (SCAPs). Materials and Methods SCAPs were transducted with ephrinB2‐lentiviral expression vector in experimental group (ephrinB2‐SCAPs) and green fluorescent protein (GFP‐SCAPs) in control group. The transduction efficiency was confirmed by real‐time PCR and western blot assays. MTT assay was performed to detect the proliferative capacity of SCAPs...

Oral Diseases

1d

Inhibition of secondary caries <em>in vitro</em> by addition of chlorhexidine to adhesive components

Publication date: Available online 22 January 2019Source: Dental MaterialsAuthor(s): Christina Boutsiouki, Roland Frankenberger, Susanne Lücker, Norbert KrämerAbstractObjectiveTo investigate secondary caries inhibition after dentine pre-treatment with 2% CHX, experimental addition of CHX in primer and adhesive of a 3-step adhesive system, and industrial addition od CHX in a 2-step adhesive system.Materials and methodSixty Class-V cavities were restored according to the adhesive protocol (n = 12):...

Dental Materials

2d

Hydrophobicity of graphene as a driving force for inhibiting biofilm formation of pathogenic bacteria and fungi

Publication date: Available online 21 January 2019Source: Dental MaterialsAuthor(s): Shruti Vidhawan Agarwalla, Kassapa Ellepola, Mariana Caldeira Ferraz da Costa, Guilhermino José Macêdo Fechine, Julien Luc Paul Morin, A.H. Castro Neto, Chaminda Jayampath Seneviratne, Vinicius RosaAbstractObjectiveTo evaluate the surface and wettability characteristics and the microbial biofilm interaction of graphene coating on titanium.MethodsGraphene was deposited on titanium (Control) via a liquid-free technique....

Dental Materials

2d

Combining Platelet Rich Fibrin With Different Bone Graft Materials: An Experimental Study On The Histopathological And Immunohistochemical Aspects Of Bone Healing

Publication date: Available online 22 January 2019Source: Journal of Cranio-Maxillofacial SurgeryAuthor(s): Fatih Karayürek, Ela Tules Kadiroğlu, Yusuf Nergis, Neslihan Coşkun Akçay, Selçuk Tunik, Berna Ersöz Kanay, Ersin UysalAbstractAimThe current study was based on the hypothesis that the use of PRF with bone graft materials might increase bone regeneration and focus on the histopathological and immunohistochemical aspects following application of PRF with autogenous graft, xenograft and B-TCP...

Cranio-Maxillofacial Surgery

2d

The preventive effect of decellularized pericardial patch against Frey's syndrome following the superficial parotidectomy

Publication date: Available online 22 January 2019Source: Journal of Cranio-Maxillofacial SurgeryAuthor(s): Chengyong Wang, Dong Wu, Chuanqing Mao, Meng Lu, Zhiyu Cai, Yongzhen Lai, Weihui ChenSummaryPurposeThis study aimed to investigate the feasibility of adopting the ThormalGEN surgical patch (a porcine decellularized pericardial patch) in the superficial parotidectomy to prevent postoperative Frey's syndrome in patients with benign parotid tumor.Materials and MethodsA total of 59 patients with...

Cranio-Maxillofacial Surgery

2d

Examination of <em>OPG</em>, <em>RANK</em>, <em>RANKL</em> and <em>HIF1A</em> polymorphisms in temporomandibular joint ankylosis patients

Publication date: Available online 21 January 2019Source: Journal of Cranio-Maxillofacial SurgeryAuthor(s): Paola Fernanda Cotait de Lucas Corso, Michelle Nascimento Meger, Igor Bassi Ferreira Petean, Juliana Feltrin de Souza, João Armando Brancher, Lea Assed Bezerra da Silva, Nelson Luis Barbosa Rebelatto, Leandro Eduardo Kluppel, Manoel Damião Sousa-Neto, Erika Calvano Küchler, Rafaela ScariotSummaryPurposeTo evaluate the association between polymorphisms in genes that regulate bone metabolism,...

Cranio-Maxillofacial Surgery

2d

Comparative evaluation of different fixation techniques for sagittal split ramus osteotomy in 10 mm advancements. Part two: finite element analysis

Publication date: Available online 21 January 2019Source: Journal of Cranio-Maxillofacial SurgeryAuthor(s): Eder Alberto Sigua-Rodriguez, Ricardo Armini Caldas, Douglas Rangel Goulart, Paulo Hemerson de Moraes, Sergio Olate, Valentim Adelino Ricardo Barão, Jose Ricardo de Albergaria-BarbosaAbstractPurposeTo evaluate three rigid, stable fixation methods for sagittal split ramus osteotomy (SSRO), using finite element analysis. The hypothesis is that a customized miniplate presents better stress concentration...

Cranio-Maxillofacial Surgery

2d

Comprehensive assessment of tranexamic acid during orthognathic surgery: a systematic review and meta-analysis of randomized, controlled trials

Publication date: Available online 21 January 2019Source: Journal of Cranio-Maxillofacial SurgeryAuthor(s): Haoming Zhao, Shaopeng Liu, Zuping Wu, Huaqiang Zhao, Chuan MaAbstractThe objective of this study was to comprehensively assess the use of tranexamic acid (TXA) during orthognathic surgery. A systematic review and meta-analysis of randomized controlled trials addressing these issues were carried out. Three electronic databases, included PubMed, Web of Science, and Cochrane Library, were searched...

Cranio-Maxillofacial Surgery

2d

Azithromycin and Erythromycin susceptibility and macrolide resistance genes in Prevotella from patients with periodontal disease

Abstract Objectives To study oral Prevotella spp. isolated from patients with chronic periodontitis, to determine their susceptibility to azithromycin and erythromycin and to screen the presence of macrolide resistance genes therein. Material and Methods Isolates with a Prevotella‐like morphology were obtained from subgingival samples of 52 patients with chronic periodontitis. Each isolate was identified to the species level by sequencing of the 16S rRNA gene. In 100 Prevotella spp. isolates,...

Oral Diseases

2d

Clinical Evaluation of Full‐arch Screw‐Retained Implant‐Supported Fixed Prostheses and Full‐arch Telescopic‐Retained Implant‐Supported Fixed Prostheses: A 5–12 Year Follow‐up Retrospective Study

Abstract Objective To assess the prostheses and implants survival rate and peri‐implantitis rate in edentulous patients treated with full‐arch screw‐retained implant‐supported fixed dental prostheses (FSIFDPs) and full‐arch telescopic‐retained implant‐supported fixed dental prostheses (FTIFDPs) over an observation period of at least 5 years. Materials and Methods From 2004 to 2012, 696 implants were inserted into 78 patients with 102 prostheses. The FSIFDP group comprised 31 patients (37...

Clinical Oral Implants Research

2d

Maxillary osteotomy complications in piezoelectric surgery compared to conventional surgical techniques: a systematic review

Publication date: Available online 21 January 2019Source: International Journal of Oral and Maxillofacial SurgeryAuthor(s): C. Thereza-Bussolaro, J. Galván Galván, C. Pachêco-Pereira, C. Flores-MirAbstractA systematic review was conducted to investigate the available evidence on maxillary complications related to piezoelectric and conventional surgery. Seven databases were searched. A total of 996 maxillary osteotomies were analysed, 864 performed with conventional tools and 132 with a piezoelectric...

Oral and Maxillofacial Surgery

2d

Maxillary osteotomy complications in piezoelectric surgery compared to conventional surgical techniques: a systematic review

A systematic review was conducted to investigate the available evidence on maxillary complications related to piezoelectric and conventional surgery. Seven databases were searched. A total of 996 maxillary osteotomies were analysed, 864 performed with conventional tools and 132 with a piezoelectric device. One hundred and fifty-six complication events were reported. The complications, in descending order of overall prevalence, were as follows: neurosensory disturbance (64.7%), haemorrhage (8.3%),...

Oral & Maxillofacial Surgery

2d

Effect of antiangiogenic targeted chemotherapy on the osseointegration of titanium implants in rabbits

Patients with cancer have recently been treated with more advanced targeted chemotherapies that have greater specificity towards the cancer cells and fewer side effects. However, the periods of treatment take longer than those of traditional cytotoxic treatments. The aim of this study was to examine the effect of antiangiogenic targeted chemotherapy on the osseointegration of titanium implants. Fourteen white New Zealand rabbits were allocated randomly into two groups of seven: the placebo control...

Oral and Maxillofacial Surgery

2d

World Workshop on Oral Medicine VII: Biomarkers predicting lymphoma in the salivary glands of patients with Sjögren's syndrome. A systematic review

Abstract Objective To conduct a systematic review of studies exploring potential biomarkers for development, course and efficacy of treatment of lymphomas in salivary glands of patients with Sjögren's syndrome. Material and Methods Eligible studies were identified through a comprehensive search of two databases, i.e. PubMed and EMBASE. Quality of included articles was assessed with the 'Quality In Prognosis Studies' (QUIPS) tool. The 'CHecklist for critical Appraisal and data extraction...

Oral Diseases

2d

Atraumatic restorative treatment—ART in early childhood caries in babies: 4 years of randomized clinical trial

Abstract Objective To assess the effectiveness of atraumatic restorative treatment (ART) performed 4 years ago in babies suffering from early childhood caries (ECC), and to compare the clinical performance of ART atraumatic restorations performed with two different high-viscosity glass ionomer cements (GIC). Materials and methods This was a longitudinal follow-up...

Clinical Oral Investigations

2d

Changes in proinflammatory gene expression in human whole blood after contact with UV-conditioned implant surfaces

Abstract Objectives The aim of this in vitro study was to assess changes in the gene expression of proinflammatory cytokines in human whole blood after contact with titanium implant surfaces conditioned by UV light. To this end, expression levels of proinflammatory cytokines were analyzed in vitro in human whole blood. Materials and methods Dental implants...

Clinical Oral Investigations

3d