Yad Ram Yadav, Yadav Nishtha, Pande Sonjjay, Parihar Vijay, Ratre Shailendra, Khare Yatin
Asian Journal of Neurosurgery 2017 12(4):585-597
Trigeminal neuralgia (TN) is a sudden, severe, brief, stabbing, and recurrent pain within one or more branches of the trigeminal nerve. Type 1 as intermittent and Type 2 as constant pain represent distinct clinical, pathological, and prognostic entities. Although multiple mechanism involving peripheral pathologies at root (compression or traction), and dysfunctions of brain stem, basal ganglion, and cortical pain modulatory mechanisms could have role, neurovascular conflict is the most accepted theory. Diagnosis is essentially clinically; magnetic resonance imaging is useful to rule out secondary causes, detect pathological changes in affected root and neurovascular compression (NVC). Carbamazepine is the drug of choice; oxcarbazepine, baclofen, lamotrigine, phenytoin, and topiramate are also useful. Multidrug regimens and multidisciplinary approaches are useful in selected patients. Microvascular decompression is surgical treatment of choice in TN resistant to medical management. Patients with significant medical comorbidities, without NVC and multiple sclerosis are generally recommended to undergo gamma knife radiosurgery, percutaneous balloon compression, glycerol rhizotomy, and radiofrequency thermocoagulation procedures. Partial sensory root sectioning is indicated in negative vessel explorations during surgery and large intraneural vein. Endoscopic technique can be used alone for vascular decompression or as an adjuvant to microscope. It allows better visualization of vascular conflict and entire root from pons to ganglion including ventral aspect. The effectiveness and completeness of decompression can be assessed and new vascular conflicts that may be missed by microscope can be identified. It requires less brain retraction.
http://ift.tt/2fVIgnK
Δευτέρα 9 Οκτωβρίου 2017
Predictive value of motor evoked potential monitoring during surgery of unruptured anterior circulation cerebral aneurysms
Yasuhiro Yamada, Yoko Kato, Mohsen Nouri, Tsukasa Ganaha, Motoki Oheda, Kohei Ishihara, Shigeta Moriya, Akiyo Sadato, Joji Inamasu, Yuichi Hirose
Asian Journal of Neurosurgery 2017 12(4):644-647
Objective and Background: Surgery of unruptured aneurysms is always a great challenge to neurovascular surgeons because no postoperative neurological deficits should be expected postoperatively as the patients are fully asymptomatic before the surgery. Here, we present our experience with selective motor evoked potential (MEP) monitoring of our patients in a 2-year time window. Patients and Methods: From 2012 to 2014, 27 patients with unruptured intracranial aneurysms were operated in our institute with the help of MEP monitoring. All patients underwent endoscope-assisted microsurgery with pre- and post-clipping indocyanine green angiography. Results: In this period, no mortality was observed, but 18.5% of the patients developed postoperative deficits which showed good recovery in all cases. Overall, MEP showed about 90% accuracy in predicting postoperative deficits. Conclusions: MEP as a part of multimodality monitoring of aneurysm surgeries is a valuable tool to improve the outcome. However, we should know its limitations as its results are not always consistent with the outcome.
http://ift.tt/2yaMQbW
Asian Journal of Neurosurgery 2017 12(4):644-647
Objective and Background: Surgery of unruptured aneurysms is always a great challenge to neurovascular surgeons because no postoperative neurological deficits should be expected postoperatively as the patients are fully asymptomatic before the surgery. Here, we present our experience with selective motor evoked potential (MEP) monitoring of our patients in a 2-year time window. Patients and Methods: From 2012 to 2014, 27 patients with unruptured intracranial aneurysms were operated in our institute with the help of MEP monitoring. All patients underwent endoscope-assisted microsurgery with pre- and post-clipping indocyanine green angiography. Results: In this period, no mortality was observed, but 18.5% of the patients developed postoperative deficits which showed good recovery in all cases. Overall, MEP showed about 90% accuracy in predicting postoperative deficits. Conclusions: MEP as a part of multimodality monitoring of aneurysm surgeries is a valuable tool to improve the outcome. However, we should know its limitations as its results are not always consistent with the outcome.
http://ift.tt/2yaMQbW
Scrotal migration of tubing: An unusual complication after ventriculo-peritoneal shunt
Monika Bawa, Saurabh Garge, Ravi Garg, Katragadda Lakshmi Narasimha Rao
Asian Journal of Neurosurgery 2017 12(4):738-740
Scrotal migration of peritoneal end of ventriculo-peritoneal (VP) shunt into the patent processus vaginalis (PPV) is a rare complication. Its exact incidence is not mentioned in the literature till date. This may be because of the rarity of this complication, and also because all previous articles related to the complication were case reports. We, in our series, had an incidence of 0.9%. This prospective study has been conducted on 437 patients in the age group of 1 month–3 years who underwent VP shunt for hydrocephalus over a period of 5 years from 2007 to 2011. The incidence of a hernia manifesting after shunt insertion has been reported to be around 16.8%. The average age of patients at the time of insertion of the shunt was 27.25 months (3–48 months). All our patients presented with swollen right sided scrotum after an average of 4 months (3–5 months) after shunt insertion. Shunt migration was more common on the right which is consistent with the incidence of hernias in children. The proper management of such cases includes repositioning of the catheter with the proper closure of the PPV. Usually, shunt revision is not required. We discuss the etiology, treatment and preventive measures of this rare entity.
http://ift.tt/2fY1OrV
Asian Journal of Neurosurgery 2017 12(4):738-740
Scrotal migration of peritoneal end of ventriculo-peritoneal (VP) shunt into the patent processus vaginalis (PPV) is a rare complication. Its exact incidence is not mentioned in the literature till date. This may be because of the rarity of this complication, and also because all previous articles related to the complication were case reports. We, in our series, had an incidence of 0.9%. This prospective study has been conducted on 437 patients in the age group of 1 month–3 years who underwent VP shunt for hydrocephalus over a period of 5 years from 2007 to 2011. The incidence of a hernia manifesting after shunt insertion has been reported to be around 16.8%. The average age of patients at the time of insertion of the shunt was 27.25 months (3–48 months). All our patients presented with swollen right sided scrotum after an average of 4 months (3–5 months) after shunt insertion. Shunt migration was more common on the right which is consistent with the incidence of hernias in children. The proper management of such cases includes repositioning of the catheter with the proper closure of the PPV. Usually, shunt revision is not required. We discuss the etiology, treatment and preventive measures of this rare entity.
http://ift.tt/2fY1OrV
Contributing factors for coagulopathy in traumatic brain injury
Ajit Shrestha, Ramesh Man Joshi, Upendra Prasad Devkota
Asian Journal of Neurosurgery 2017 12(4):648-652
Context: In traumatic brain injury patients, coagulation disorder causes secondary brain injury, thereby increasing mortality and morbidity. Aims: The aim of this study is to identify the factors responsible for coagulopathy in traumatic brain injury. Settings and Design: This prospective longitudinal study from June 2012 included 100 patients with moderate and severe head injury presenting to National Institute of Neurological and Allied Sciences, Kathmandu, over 1-year period. Subjects and Methods: Patients were evaluated for the development of coagulopathy, defined as collectively three abnormal hemostatic parameters, and associated risk factors for coagulopathy. They were then analyzed for correlation with coagulopathy. Statistical Analysis Used: SPSS version 16 was used for the analysis of data. For identification of contributing factors, a stepwise logistic regression analysis was performed, including the factors with P < 0.05 from the analysis. Results: Among the 100 patients, coagulopathy was present in 63% of cohort. Forty-three patients had severe head injury, and 76.7% (n = 33) of them had coagulopathy compared to 52.7% (n = 30) in 57 patients with moderate head injury (P = 0.013). Statistically significant correlation with coagulopathy was present with polytrauma, severity of head injury, blood transfusion, surgical intervention, and Marshall's classification of CT of the head; however, stepwise logistic regression analysis showed that blood transfusion, surgical intervention, polytrauma, and severity of head injury were significant independent variables responsible for the development of coagulopathy. Conclusions: Traumatic brain injury is complicated with coagulopathy in up to 63% of patients. Blood transfusion, surgical intervention, polytrauma, and severity of head injury are significant independent variables responsible for coagulopathy.
http://ift.tt/2yaYrI1
Asian Journal of Neurosurgery 2017 12(4):648-652
Context: In traumatic brain injury patients, coagulation disorder causes secondary brain injury, thereby increasing mortality and morbidity. Aims: The aim of this study is to identify the factors responsible for coagulopathy in traumatic brain injury. Settings and Design: This prospective longitudinal study from June 2012 included 100 patients with moderate and severe head injury presenting to National Institute of Neurological and Allied Sciences, Kathmandu, over 1-year period. Subjects and Methods: Patients were evaluated for the development of coagulopathy, defined as collectively three abnormal hemostatic parameters, and associated risk factors for coagulopathy. They were then analyzed for correlation with coagulopathy. Statistical Analysis Used: SPSS version 16 was used for the analysis of data. For identification of contributing factors, a stepwise logistic regression analysis was performed, including the factors with P < 0.05 from the analysis. Results: Among the 100 patients, coagulopathy was present in 63% of cohort. Forty-three patients had severe head injury, and 76.7% (n = 33) of them had coagulopathy compared to 52.7% (n = 30) in 57 patients with moderate head injury (P = 0.013). Statistically significant correlation with coagulopathy was present with polytrauma, severity of head injury, blood transfusion, surgical intervention, and Marshall's classification of CT of the head; however, stepwise logistic regression analysis showed that blood transfusion, surgical intervention, polytrauma, and severity of head injury were significant independent variables responsible for the development of coagulopathy. Conclusions: Traumatic brain injury is complicated with coagulopathy in up to 63% of patients. Blood transfusion, surgical intervention, polytrauma, and severity of head injury are significant independent variables responsible for coagulopathy.
http://ift.tt/2yaYrI1
Contrecoup extradural hematoma with coronal suture diastasis
Pratap Chandra Nath, Sudhansu Sekhar Mishra, Manmath Kumar Dhir, Rama Chandra Deo, Bikash Ranjan Behera, Sitansu Kumar Rout
Asian Journal of Neurosurgery 2017 12(4):751-753
Extradural hematoma (EDH) generally occurs in the site of impact, that is, coup injury site. EDH is associated with fracture of skull in many a times due to direct impact. However, EDH in counter coup site is a rare occurrence. Hardly, yet, 12 cases have been reported including this case. Here, we reported a case of a 22-year-old male of contrecoup acute EDH who had sustained head injury due to fall from bike. Physical examination revealed direct impact at the left occipito-parietal region with laceration of scalp and bruise with transient loss of consciousness at the time of injury. There was no evidence of impact on the right side of his head. Computed tomography scan revealed an EDH in the right fronto-temporal region without any bone fracture on bone windows. On intraoperative exploration, it is found that there was coronal suture diastasis with small subgaleal hematoma and right fronto-temporal extradural hematoma. The evacuation of EDH was done. The patient discharged on 3rd postoperative day. It can be concluded that direct impact on head causes exactly opposite hit of brain, and compensatory rebound causes a negative pressure in between the layers causing disruption of vessels and potential accumulation of blood. This mechanism mostly favors for acute subdural hematoma due to compact attachment of dura with cranium. However, in this case, diastasis of coronal suture due to transmitted force is the cause of accumulation of blood extradurally in relatively loosely adhered dura in fronto-temporal region where a potential negative pressure space is created by contrecoup injury.
http://ift.tt/2fY1IR5
Asian Journal of Neurosurgery 2017 12(4):751-753
Extradural hematoma (EDH) generally occurs in the site of impact, that is, coup injury site. EDH is associated with fracture of skull in many a times due to direct impact. However, EDH in counter coup site is a rare occurrence. Hardly, yet, 12 cases have been reported including this case. Here, we reported a case of a 22-year-old male of contrecoup acute EDH who had sustained head injury due to fall from bike. Physical examination revealed direct impact at the left occipito-parietal region with laceration of scalp and bruise with transient loss of consciousness at the time of injury. There was no evidence of impact on the right side of his head. Computed tomography scan revealed an EDH in the right fronto-temporal region without any bone fracture on bone windows. On intraoperative exploration, it is found that there was coronal suture diastasis with small subgaleal hematoma and right fronto-temporal extradural hematoma. The evacuation of EDH was done. The patient discharged on 3rd postoperative day. It can be concluded that direct impact on head causes exactly opposite hit of brain, and compensatory rebound causes a negative pressure in between the layers causing disruption of vessels and potential accumulation of blood. This mechanism mostly favors for acute subdural hematoma due to compact attachment of dura with cranium. However, in this case, diastasis of coronal suture due to transmitted force is the cause of accumulation of blood extradurally in relatively loosely adhered dura in fronto-temporal region where a potential negative pressure space is created by contrecoup injury.
http://ift.tt/2fY1IR5
Profiling cognitive deficits in intra-axial and extra-axial tumors using addenbrooke's cognitive examination as a screening tool: An Indian experience
Sandhya Cherkil, Dilip Panikar, Deepak Kuttikkattu Soman
Asian Journal of Neurosurgery 2017 12(4):653-658
Background: Tumors of the brain, whether intra- or extra-axial, results in cognitive deficits. The aim of the present study was to profile cognitive deficits using Addenbrooke's Cognitive Examination-Malayalam (ACE-M) as a screen and to determine the sensitivity and specificity of the same. Methods: Seventy-four drug naïve patients diagnosed to have brain tumors were assessed for cognitive functioning using ACE-M before surgery. Results: Patients with high-grade intra-axial tumors showed a significant association on the cognitive domains of registration (0.04), recall (0.01), and visuospatial functioning (0.02). Gender showed an association between registration (0.02) and verbal fluency (0.02) with females performing better while education was significantly associated with retrograde or remote memory (0.00) with college-educated sample performing better. Significance was assumed at P < 0.05. In extra-axial tumors, laterality had a single association with recall (0.02). Males showed a significant cognitive decline on the cognitive domains of attention (0.02), recall (0.05), naming (0.02), and language functions (0.01). College educated group performed better on registration (0.01), recall (0.09), naming (0.00), and visuospatial functioning (0.00). The area under the receiver operating characteristic curve was estimated as 0.75, which indicates fairly good discriminative ability with a cut off of 71/100; sensitivity at 77.3 and specificity fixed at 67. Conclusions: ACE-M is capable of bringing out cognitive deficits along with a number of cognitive domains in patients with intra- and extra-axial tumors in the capacity of a screen, with fairly good levels of sensitivity and specificity.
http://ift.tt/2yaQy5c
Asian Journal of Neurosurgery 2017 12(4):653-658
Background: Tumors of the brain, whether intra- or extra-axial, results in cognitive deficits. The aim of the present study was to profile cognitive deficits using Addenbrooke's Cognitive Examination-Malayalam (ACE-M) as a screen and to determine the sensitivity and specificity of the same. Methods: Seventy-four drug naïve patients diagnosed to have brain tumors were assessed for cognitive functioning using ACE-M before surgery. Results: Patients with high-grade intra-axial tumors showed a significant association on the cognitive domains of registration (0.04), recall (0.01), and visuospatial functioning (0.02). Gender showed an association between registration (0.02) and verbal fluency (0.02) with females performing better while education was significantly associated with retrograde or remote memory (0.00) with college-educated sample performing better. Significance was assumed at P < 0.05. In extra-axial tumors, laterality had a single association with recall (0.02). Males showed a significant cognitive decline on the cognitive domains of attention (0.02), recall (0.05), naming (0.02), and language functions (0.01). College educated group performed better on registration (0.01), recall (0.09), naming (0.00), and visuospatial functioning (0.00). The area under the receiver operating characteristic curve was estimated as 0.75, which indicates fairly good discriminative ability with a cut off of 71/100; sensitivity at 77.3 and specificity fixed at 67. Conclusions: ACE-M is capable of bringing out cognitive deficits along with a number of cognitive domains in patients with intra- and extra-axial tumors in the capacity of a screen, with fairly good levels of sensitivity and specificity.
http://ift.tt/2yaQy5c
Combined use of intraoperative indocyanine green and dynamic angiography in rotational vertebral artery occlusion
Nauman Chaudhry, Brandon Gerard Gaynor, Sudheer Ambekar, Mohamed Samy Elhammady
Asian Journal of Neurosurgery 2017 12(4):763-765
Rotational vertebral artery occlusion (RVAO) is a well-documented surgically amenable cause of vertebrobasilar insufficiency. Traditionally, patients have been imaged using dynamic rotational angiography. We report a case of RVAO in which intraoperative indocyanine green angiography (ICGA) was used to confirm adequate surgical decompression of the VA. A 57-year-old female who presented with multiple episodes of syncope provoked by turning her head to the right. Rotational dynamic angiography revealed a dominant right VA that became occluded at the level of C5/6 with head rotation to the right. The patient underwent successful surgical decompression of the VA via an anterior cervical approach. ICGA demonstrated VA patency with head rotation. This was further confirmed by intraoperative dynamic catheter angiography. To the best of our knowledge, we present the first use of ICG combined with intra-operative dynamic rotational angiography to document the adequacy surgical decompression of the VA in a patient with RVAO.
http://ift.tt/2fY1EAP
Asian Journal of Neurosurgery 2017 12(4):763-765
Rotational vertebral artery occlusion (RVAO) is a well-documented surgically amenable cause of vertebrobasilar insufficiency. Traditionally, patients have been imaged using dynamic rotational angiography. We report a case of RVAO in which intraoperative indocyanine green angiography (ICGA) was used to confirm adequate surgical decompression of the VA. A 57-year-old female who presented with multiple episodes of syncope provoked by turning her head to the right. Rotational dynamic angiography revealed a dominant right VA that became occluded at the level of C5/6 with head rotation to the right. The patient underwent successful surgical decompression of the VA via an anterior cervical approach. ICGA demonstrated VA patency with head rotation. This was further confirmed by intraoperative dynamic catheter angiography. To the best of our knowledge, we present the first use of ICG combined with intra-operative dynamic rotational angiography to document the adequacy surgical decompression of the VA in a patient with RVAO.
http://ift.tt/2fY1EAP
Comparison between ventriculosubgaleal shunt and extraventricular drainage to treat acute hydrocephalus in adults
Low Siaw Nee, Rahmat Harun, Pulivendhan Sellamuthu, Zamzuri Idris
Asian Journal of Neurosurgery 2017 12(4):659-663
Context: Hydrocephalus, due to subarachnoid or intraventricular hemorrhage (IVH), meningitis, or tumor compression, is usually transient and may resolve after treatment. There are several temporary methods of cerebrospinal fluid (CSF) diversion, none of it is superior to the other, and the decision is based on its various etiologies and factors. Ventriculosubgaleal shunt (VSGS) is one of those temporary measures, which is a simple and rapid CSF decompression method without causing electrolyte and nutritional losses. Aims: The aim is to study the efficacy of VSGS for temporary CSF diversion, compared to extraventricular drainage (EVD) in adult hydrocephalus patients; to evaluate the outcome in terms of avoiding a permanent shunt, and to look for incidences of their complications. Settings and Design: This was a retrospective observational study. Subjects and Methods: The data were acquired from case notes of fifty patients with acute hydrocephalus: 26 secondary to IVH, 10 from aneurysm rupture, 8 posttrauma, and 6 from infection. All these patients had undergone CSF diversion in Hospital Queen Elizabeth II, Sabah, Malaysia, between 2013 and 2015. The patients were followed up from the date of treatment until the resolution of hydrocephalus, where parameters such as shunt dependency and complications were documented. Statistical Analysis Used: All analyses were carried out using Statistical Packages for the Social Sciences Version 22.0. Chi-squared test or Fisher's exact test is used for univariate analysis of categorical variables. Results: A total of 21 (42%) patients underwent EVD insertion and 29 (58%) underwent VSGS insertion. Thirty-seven (74%) patients did not require a permanent shunt; 24 (64.8%) of them were from the VSGS group (P = 0.097). EVD had more intracranial complications (44.1%) compared with VSGS (23.5%), with a statistically significant P = 0.026. Conclusions: VSGS is a safe and viable option for adult hydrocephalus patients, with the possibility of continuation of the treatment for such patients in nonneurosurgical centers, as opposed to patients with EVDs. Furthermore, even though this method had no statistical difference in avoiding a permanent ventriculoperitoneal shunt, VSGS has statistically significant less intracranial complications compared with EVD.
http://ift.tt/2yaofnu
Asian Journal of Neurosurgery 2017 12(4):659-663
Context: Hydrocephalus, due to subarachnoid or intraventricular hemorrhage (IVH), meningitis, or tumor compression, is usually transient and may resolve after treatment. There are several temporary methods of cerebrospinal fluid (CSF) diversion, none of it is superior to the other, and the decision is based on its various etiologies and factors. Ventriculosubgaleal shunt (VSGS) is one of those temporary measures, which is a simple and rapid CSF decompression method without causing electrolyte and nutritional losses. Aims: The aim is to study the efficacy of VSGS for temporary CSF diversion, compared to extraventricular drainage (EVD) in adult hydrocephalus patients; to evaluate the outcome in terms of avoiding a permanent shunt, and to look for incidences of their complications. Settings and Design: This was a retrospective observational study. Subjects and Methods: The data were acquired from case notes of fifty patients with acute hydrocephalus: 26 secondary to IVH, 10 from aneurysm rupture, 8 posttrauma, and 6 from infection. All these patients had undergone CSF diversion in Hospital Queen Elizabeth II, Sabah, Malaysia, between 2013 and 2015. The patients were followed up from the date of treatment until the resolution of hydrocephalus, where parameters such as shunt dependency and complications were documented. Statistical Analysis Used: All analyses were carried out using Statistical Packages for the Social Sciences Version 22.0. Chi-squared test or Fisher's exact test is used for univariate analysis of categorical variables. Results: A total of 21 (42%) patients underwent EVD insertion and 29 (58%) underwent VSGS insertion. Thirty-seven (74%) patients did not require a permanent shunt; 24 (64.8%) of them were from the VSGS group (P = 0.097). EVD had more intracranial complications (44.1%) compared with VSGS (23.5%), with a statistically significant P = 0.026. Conclusions: VSGS is a safe and viable option for adult hydrocephalus patients, with the possibility of continuation of the treatment for such patients in nonneurosurgical centers, as opposed to patients with EVDs. Furthermore, even though this method had no statistical difference in avoiding a permanent ventriculoperitoneal shunt, VSGS has statistically significant less intracranial complications compared with EVD.
http://ift.tt/2yaofnu
Concurrent multilevel spinal intra-medullary with extensive intracranial tuberculomas: A rare case report
Shyam Sundar Krishnan, Bhavinkumar Rameshchandra Patel, Madabushi Chakravarthy Vasudevan
Asian Journal of Neurosurgery 2017 12(4):774-778
Disseminated tuberculomas in the brain and spinal cord are rare. To the best of our knowledge, only nine cases of spinal intra-medullary tuberculomas with cranial involvement have been reported till date. However, involvement of all levels in the spinal cord, brain stem with pan lobar involvement of the cerebrum and cerebellum has not been reported so far. We present such a case of a 12-year-old boy with history of pulmonary tuberculosis, who presented with gradual onset of quadriparesis and generalized seizures. We have discussed the unusual clinical presentation and the temporal changes in magnetic resonance imaging features along with clinical response to treatment. In cases reported so far, the plan of surgical versus medical management has been opted for variably, in cases of spinal intra-medullary involvement with acute neurological deficit. The decision is even more difficult in multilevel spinal intra-medullary tuberculomas. Our patient showed good clinico-radiological improvement with medical management.
http://ift.tt/2fVHSpi
Asian Journal of Neurosurgery 2017 12(4):774-778
Disseminated tuberculomas in the brain and spinal cord are rare. To the best of our knowledge, only nine cases of spinal intra-medullary tuberculomas with cranial involvement have been reported till date. However, involvement of all levels in the spinal cord, brain stem with pan lobar involvement of the cerebrum and cerebellum has not been reported so far. We present such a case of a 12-year-old boy with history of pulmonary tuberculosis, who presented with gradual onset of quadriparesis and generalized seizures. We have discussed the unusual clinical presentation and the temporal changes in magnetic resonance imaging features along with clinical response to treatment. In cases reported so far, the plan of surgical versus medical management has been opted for variably, in cases of spinal intra-medullary involvement with acute neurological deficit. The decision is even more difficult in multilevel spinal intra-medullary tuberculomas. Our patient showed good clinico-radiological improvement with medical management.
http://ift.tt/2fVHSpi
Isolation and Establishment of Gustatory Cell Lines in Patients Operated on for Cancer of the Mobile Tongue - ImmortTasteCELL
Condition: Cancer of the Tongue
Intervention: Procedure: Sample of cancerous epithelium from the tongue
Sponsor: Centre Hospitalier Universitaire Dijon
Recruiting
http://ift.tt/2yBgB7e
Intervention: Procedure: Sample of cancerous epithelium from the tongue
Sponsor: Centre Hospitalier Universitaire Dijon
Recruiting
http://ift.tt/2yBgB7e
Blood cadmium levels associated with short distant metastasis-free survival time in invasive breast cancer
Abstract
Distant metastasis is strongly associated with poor prognosis of breast cancer. Cadmium (Cd) exposure was previously found associated with breast cancer incidence. We explored the associations of blood cadmium levels (BCLs) and clinicopathologic characteristics with invasive breast cancer distant metastasis. Blood samples were collected and analyzed for BCLs by graphite-furnace atomic absorption spectrometry. Clinicopathologic characteristics, including basic clinical information and tumor characteristics, were obtained from medical records. Breast cancer distant metastasis-free survival (DMFS) time was calculated at follow-up. The associations of BCLs and clinicopathologic characteristics with DMFS time were examined by Kaplan–Meier method and Cox regression analysis, and associations between BCLs and tumor characteristics were also explored. Blood Cd level was positively associated with distant metastasis, clinical stage, BMI, and age. On univariate analysis, older age at diagnosis, family history of breast cancer, high N classification and clinical stage, positivity for human epidermal growth factor receptor 2, and high BCLs were associated with short DMFS time. On multivariate analysis model, older age at diagnosis, family history of breast cancer, high N classification, and BCLs were predictors for breast cancer distant metastasis. BCLs were a risk factor for short DMFS time of invasive breast cancer. BCLs and some clinicopathologic factors affect breast cancer distant metastasis, which needs further epidemiological and experimental studies to confirm.
http://ift.tt/2yays3p
Rapid changes in brain aromatase activity in the female quail brain following expression of sexual behavior
Abstract
In male quail, estrogens produced in the brain (neuroestrogens) exert a dual action on male sexual behavior: they increase sexual motivation within minutes via mechanisms activated at the membrane but facilitate sexual performance by slower, presumably nuclear-initiated, mechanisms. Recent work indicates that neuroestrogens are also implicated in the control of female sexual motivation despite the presence of high circulating concentrations of estrogens of ovarian origin. Interestingly aromatase activity (AA) in the male brain is regulated in time domains corresponding to the slow "genomic" and faster "non-genomic" modes of action of estrogens. Furthermore, rapid changes in brain AA are observed in males after sexual interactions with a female. In this study, we investigated whether similar rapid changes in brain AA are observed in females allowed to interact sexually with males. A significant decrease in AA was observed in the medial preoptic nucleus after interactions that lasted 2, 5 or 10 min but this decrease was no longer significant after 15 min of interaction. In the bed nucleus of the stria terminalis, a progressive decline of average AA was observed between 2 and 15 min but it never reached statistical significance. AA in this nucleus was however negatively correlated with the sexual receptivity of the female. These data indicate that sexual interactions affect brain AA in females as in males in an anatomically specific manner and suggest that rapid changes in brain estrogens production could also modulate female sexual behavior.
This article is protected by copyright. All rights reserved.
http://ift.tt/2yS3omg
Factors affecting palatability of four submerged macrophytes for grass carp Ctenopharyngodon idella
Abstract
Grass carp can weaken the growth and reproductive capacity of submerged macrophytes by consuming valuable tissues, but factors affecting palatability of submerged macrophytes for grass carp rarely are considered. In this study, relative consumption rate of grass carp with regard to submerged macrophytes was in the following order: Hydrilla verticillata > Vallisneria natans > Ceratophyllum demersum > Myriophyllum spicatum. Firmness of macrophytes was in the following order: M. spicatum > C. demersum > H. verticillata = V. natans, whereas shear force was M. spicatum > C. demersum > H. verticillata > V. natans. After crude extracts of M. spicatum were combined with H. verticillata, grass carp fed on fewer macrophyte pellets that contained more plant secondary metabolites (PSMs). This indicated that structure and PSMs affected palatability of macrophytes.
PSMs do not contribute to reduction in palatability through inhibition of intestinal proteinases activity, but they can cause a decrease in the abundance of Exiguobacterium, Acinetobacter-yielding proteases, lipases, and cellulose activity, which in turn can weaken the metabolic capacity of grass carp and adversely affect their growth. Thus, the disadvantages to the growth and development of grass carp caused by PSMs may drive grass carp to feed on palatable submerged macrophytes with lower PSMs.
http://ift.tt/2yzGZyc
Late breast implant infections: a delayed MRSA infection from hematogenous spread in an intravenous drug user
Abstract
Infection following breast augmentation is a rare event, with an incidence of 0–4% in most studies. We present the case of a methicillin-resistant Staphylococcus aureus bilateral breast implant infection in a 42-year-old female, 19 years after submuscular saline breast augmentation. Her history was significant for intravenous drug abuse and concomitant osteomyelitis of the sternum and manubrium. She underwent washout and debridement, with explantation of the breast implants, and long-term intravenous vancomycin. This represents the longest reported occurrence of late infection in breast augmentation and the only report of a breast implant infection due to hematogenous spread/seeding from intravenous drug abuse.
Level of Evidence: Level V, risk study.
http://ift.tt/2xsXh79
Scholar : These new articles for Critical Discourse Studies are available online
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Anxiolytic effect of fatty acids and terpenes fraction from Aloysia triphylla: Serotoninergic, GABAergic and glutamatergic implications
Publication date: December 2017
Source:Biomedicine & Pharmacotherapy, Volume 96
Author(s): Enrique Jiménez-Ferrer, Mayra Alejandra Santillán-Urquiza, Elian Alegría-Herrera, Alejandro Zamilpa, Carmen Noguerón-Merino, Jaime Tortoriello, Victor Navarro-García, Margarita Avilés-Flores, Macrina Fuentes-Mata, Maribel Herrera-Ruiz
Aloysia triphylla (Verbenaceae) is an aromatic medicinal plant, and it is used for the treatment of "nervous" problems as, "sadness" and "nervousness". While, there are no reports about its pharmacological activity in animal models. The objective of this work was to evaluate the anxiolytic effect of the extracts and fractions of this species and to measure the interaction of the most active fraction with serotonergic, glutamatergic and GABAergic drugs.An elevated plus maze test was carried ought where the methanol (AtM), dicloromethane (AtD) and hexanic (AtH) extracts presented anxiolytic activity in mice when exposed to the test. Also, different fractions obtained from the AtD were evaluated (AtF1, AtF2 and AtF3, 15mg/kg), and showed that fraction AtF1 possessed the anxiolytic activity, in the same model. Then, AtF1 was co-administered with different drugs, which act on GABAergic (bicuculline, picrotoxin, pentylenetetrazol, baclofen and phaclofen), or serotononinergic (DOI, 8-OH-DPAT, WAY 100635 and ketanserine) or glutamatergic (NMDA, MPEP and MK-801) systems. The anxiolytic activity of AtF1 was modified by GABAergic and serotoninergic drugs. Chemical analysis of this fraction by using GC–MS, showed that it contains hexadecanoic acid, hexadecanoic acid methyl ester, octadecanoic acid methyl ester, eicosanoic acid methyl ester, vitamin E, α-amiryn, campesterol, sitosterol, stigmastan-2,22, dien-3-ol (4) and stigmasta 5, 24 (28) dien-3-ol.
http://ift.tt/2hY1Iog
Protective effect of carvacrol on acetic acid-induced colitis
Publication date: December 2017
Source:Biomedicine & Pharmacotherapy, Volume 96
Author(s): Marilia Trindade de Santana Souza, Daiane Franco Teixeira, Janaíne Prata de Oliveira, Alan Santos Oliveira, Lucindo José Quintans-Júnior, Cristiane Bani Correa, Enilton Aparecido Camargo
The pharmacological therapy for inflammatory bowel diseases continues to be problematic, and requires new alternative options. In this study, we tested the hypothesis that carvacrol (CAR), a phenolic monoterpene with anti-inflammatory and antioxidant activities, can treat experimental colitis in mice. C57BL/6 mice (n=8/group) were subjected to intrarectal administration of acetic acid (5%) to induce colitis. Mice were pretreated with CAR (25, 50 or 100mg/kg, p.o.) every 12h for three days prior to the induction. Abdominal hyperalgesia, macroscopic and microscopic colon damage, myeloperoxidase (MPO) activity, tumor necrosis factor (TNF)-α and interleukin (IL)-1β levels, oxidative stress markers, and antioxidant enzyme activities were evaluated. Pretreatment with all doses of CAR significantly decreased abdominal hyperalgesia and colon MPO activity and TNF-α and IL-1β levels. A reduction in macroscopic and microscopic damage (p<0.05) was observed at doses of 50 and 100mg/kg CAR. Pretreatment with CAR significantly reduced lipid peroxidation (for all doses) and increased sulfhydryl groups (at 100mg/kg). This effect was accompanied by a significant increase in catalase, superoxide dismutase, and glutathione peroxidase activities. These findings indicate that CAR protected mice from acetic acid-induced colitis by reducing inflammatory, nociceptive, and oxidative damages.
Graphical abstract
http://ift.tt/2g2kfPA
Clusia hilariana and Eugenia uniflora as bioindicators of atmospheric pollutants emitted by an iron pelletizing factory in Brazil
Abstract
The objectives of this work were to evaluate if the pollution emitted by the pelletizing factory causes visual symptoms and/or anatomical changes in exposed Eugenia uniflora and Clusia hilariana, in active biomonitoring, at different distances from a pelletizing factory. We characterize the symptomatology, anatomical, and histochemistry alterations induced in the two species. There was no difference in the symptomatology in relation to the different distances of the emitting source. The foliar symptoms found in C. hilariana were chlorosis, necrosis, and foliar abscission and, in E. uniflora, were observed necrosis punctuais, purple spots in the leaves, and increase in the emission of new leaves completely purplish. The two species presented formation of a cicatrization tissue. E. uniflora presented reduction in the thickness of leaf. In C. hilariana, it was visualized hyperplasia of the cells and the adaxial epidermis did not appear collapsed due to thick cuticle and cuticular flanges. Leaves of C. hilariana showed positive staining for iron, protein, starch, and phenolic compounds. E. uniflora showed positive staining for total phenolic compounds and starch. Micromorphologically, there was accumulation of particulate matter on the leaf surface, obstruction of the stomata, and scaling of the epicuticular wax in both species. It was concluded that the visual and anatomical symptoms were efficient in the diagnosis of the stress factor. C. hilariana and E. uniflora showed to be good bioindicators of the atmospheric pollutants emitted by the pelletizing factory.
http://ift.tt/2gnC3lb
Irradiance, as well as body site and timing of readings, is important in determining ultraviolet A minimal erythemal dose: reply from the authors
Abstract
We would like to thank Eadie et al.1 for their interest in our study recently published in the BJD.2 Eadie et al.1 suggested that the discrepancies between the ultraviolet (UV)A1 minimal erythema doses (MEDs) observed in our recent study2 and the doses previously reported by Beattie et al.3 were mainly due to differences in irradiance, anatomical site and timing of MED readings.
This article is protected by copyright. All rights reserved.
http://ift.tt/2go4oIl
Reply to ‘Impact of biologic therapies on risk of major adverse cardiovascular events in patients with psoriasis: systematic review and meta-analysis of randomized controlled trials’: reply from the authors
Abstract
We would like to thank Drs Amin, No and Wu for their interest in our recent publication.2 We are pleased to note, in particular, how much we agree on one fundamental point - there is a need for future well-designed studies that involve larger numbers of patients and longer durations of treatment exposure in order to better assess the impact of biologic therapies on the risk of major adverse cardiovascular events (MACEs) in patients with psoriasis.
This article is protected by copyright. All rights reserved.
http://ift.tt/2y9KpGX
Utility of Pit-1 Immunostaining in Distinguishing Pituitary Adenomas of Primitive Differentiation from Null Cell Adenomas
Abstract
Pit-1 immunostaining is not routinely used in the characterization of pituitary adenomas, and its utility in distinguishing adenomas dedicated towards the lactotroph, somatotroph, and thyrotroph lineage from null cell adenomas warrants further evaluation. Pituitary adenomas that were negative for expression of a basic panel of hormonal markers (ACTH, prolactin, and growth hormone) were further evaluated for TSH, SF-1, and Pit-1 expression using a tissue microarray. Among the 147 identified pituitary adenomas that were negative for ACTH, prolactin, growth hormone, and TSH, expression of SF-1 was present in 68 cases (46%). Of the remaining 72 cases with sufficient tissue for further analysis, four were Pit-1 positive (6% of the adenomas negative for ACTH, prolactin, growth hormone, TSH, and SF-1); the remaining 68 were potentially null cell adenomas. Two of the Pit-1-positive adenomas displayed a paranuclear CAM 5.2 staining pattern suggestive of a sparsely granulated somatotroph adenoma; however, only one case contained fibrous bodies within a majority of the adenoma cells. Our data suggests that Pit-1 can be utilized as a second tier immunostain in cases of clinically non-functioning adenomas that are immunonegative for ACTH, prolactin, growth hormone, TSH, and SF-1 in order to further segregate rare cases of Pit-1-positive adenomas from null cell adenomas. Pit-1 immunostaining can recognize rare cases of sparsely granulated somatotroph adenomas that appear immunonegative for growth hormone, as well as rare cases of other Pit-1-positive adenomas that are negative for Pit-1 lineage hormones. Overall, pituitary adenomas of the Pit-1 lineage that do not produce prolactin, growth hormone, or TSH are rare, with only four cases identified in the current study.
http://ift.tt/2yQOwVu
Κυριακή 8 Οκτωβρίου 2017
Transporter for sulfated steroid hormones in the testis − expression pattern, biological significance and implications for fertility in men and rodents
Publication date: Available online 7 October 2017
Source:The Journal of Steroid Biochemistry and Molecular Biology
Author(s): D. Fietz
In various tissues, steroid hormones may be sulfated, glucuronidated or otherwise modified. For a long time, these hydrophilic molecules have been considered to be merely inactive metabolites for excretion via bile or urine. Nevertheless, different organs such as the placenta and breast tissue produce large amounts of sulfated steroids. After the discovery of the enzyme steroid sulfatase, which is able to re-activate sulfated steroids, these precursor molecules entered the focus of interest again as a local supply for steroid hormone synthesis with a prolonged half-life compared to their unconjugated counterparts. The first descriptions of this so-called sulfatase pathway in the placenta and breast tissue (with special regards to hormone-dependent breast cancer) were quickly followed by studies of steroid sulfate production and function in the testis. These hydrophilic molecules may not permeate the cell membrane by diffusion in the way that unbound steroids can, but need to be transported through the plasma membrane by transport systems. In the testis, a functional sulfatase pathway requires the expression of specific uptake carrier and efflux transporters in testicular cells, i.e. Sertoli, Leydig and germ cells. Main focus has to be placed on Sertoli cells, as these cells build up the blood-testis barrier.In this review, an overview of carrier expression pattern in the human as well as rodent testis is provided with special interest towards implications on fertility.
http://ift.tt/2xs2osM
A new practical classification for spatial distribution and morphology of human hair: Ahmad's LGMA classification
Summary
Background
The human hair is a complex structure with a diversity within itself. There is a wide variation in distribution and spatial arrangement of hairs.
Methods
The current classification was developed on the basis of high-resolution photographs of adult human scalp.
Results
On the observation, the new classification consisted of 4 categories.
Conclusions
It will help the hair restoration surgeons and dermatologists to identify the various pattern and also to help in deciding the appropriate size of the punch in FUE.
http://ift.tt/2kywpRH
Investigating the role of chemical and physical processes on organic aerosol modelling with CAMx in the Po Valley during a winter episode
Source:Atmospheric Environment
Author(s): A. Meroni, G. Pirovano, S. Gilardoni, G. Lonati, C. Colombi, V. Gianelle, M. Paglione, V. Poluzzi, G.M. Riva, A. Toppetti
Traditional aerosol mechanisms underestimate the observed organic aerosol concentration, especially due to the lack of information on secondary organic aerosol (SOA) formation and processing. In this study we evaluate the chemical and transport model CAMx during a one-month in winter (February 2013) over a 5 km resolution domain, covering the whole Po valley (Northern Italy). This works aims at investigating the effects of chemical and physical atmospheric processing on modeling results and, in particular, to evaluate the CAMx sensitivity to organic aerosol (OA) modeling schemes: we will compare the recent 1.5D-VBS algorithm (CAMx-VBS) with the traditional Odum 2-product model (CAMx-SOAP). Additionally, the thorough diagnostic analysis of the reproduction of meteorology, precursors and aerosol components was intended to point put strength and weaknesses of the modeling system and address its improvement.Firstly, we evaluate model performance for criteria PM concentration. PM10 concentration was underestimated both by CAMx-SOAP and even more by CAMx-VBS, with the latter showing a bias ranging between -4.7 and -7.1 μg m−3. PM2.5 model performance was to some extent better than PM10, showing a mean bias ranging between -0.5 μg m−3 at rural sites and -5.5 μg m−3 at urban and suburban sites. CAMx performance for OA was clearly worse than for the other PM compounds (negative bias ranging between -40% and -75%). The comparisons of model results with OA sources (identified by PMF analysis) shows that the VBS scheme underestimates freshly emitted organic aerosol while SOAP overestimates. The VBS scheme correctly reproduces biomass burning (BBOA) contributions to primary OA concentrations (POA). In contrast VBS slightly underestimates the contribution from fossil-fuel combustion (HOA), indicating that POA emissions related to road transport are either underestimated or associated to higher volatility classes. The VBS scheme under-predictes the SOA too, but to a lesser extent than CAMx-SOAP. SOA underestimation can be related to corresponding underestimation of either aging processes or precursor emissions. This indicates that improvements in the emission inventories for semi- and intermediate-volatility organic compounds are needed for further progress in this area. Finally, the comparison between modeled and observed SOA sources points out the urgency to include processing of OA in particle water phase into SOA formation mechanisms, to reconcile model results and observations.
http://ift.tt/2wHOPkO
Quantitative assessment of source contributions to PM2.5 on the west coast of Peninsular Malaysia to determine the burden of Indonesian peatland fire
Source:Atmospheric Environment
Author(s): Yusuke Fujii, Susumu Tohno, Norhaniza Amil, Mohd Talib Latif
Almost every dry season, peatland fires occur in Sumatra and Kalimantan Inlands. Dense smoke haze from Indonesian peatland fires (IPFs) causes impacts on health, visibility, transport and regional climate in Southeast Asian countries such as Indonesia, Malaysia, and Singapore. Quantitative knowledge of IPF source contribution to ambient aerosols in Southeast Asia (SEA) is so useful to make appropriate suggestions to policy makers to mitigate IPF-induced haze pollution. However, its quantitative contribution to ambient aerosols in SEA remains unclarified. In this study, the source contributions to PM2.5 were determined by the Positive Matrix Factorization (PMF) model with annual comprehensive observation data at Petaling Jaya on the west coast of Peninsular Malaysia, which is downwind of the IPF areas in Sumatra Island, during the dry (southwest monsoon: June–September) season. The average PM2.5 mass concentration during the whole sampling periods (Aug 2011–Jul 2012) based on the PMF and chemical mass closure models was determined as 20–21 μg m−3. Throughout the sampling periods, IPF contributed (on average) 6.1–7.0 μg m−3 to the PM2.5, or ∼30% of the retrieved PM2.5 concentration. In particular, the PM2.5 was dominantly sourced from IPF during the southwest monsoon season (51–55% of the total PM2.5 concentration on average). Thus, reducing the IPF burden in the PM2.5 levels would drastically improve the air quality (especially during the southwest monsoon season) around the west coast of Peninsular Malaysia.
http://ift.tt/2y4vVpM
Toward multi-day-ahead forecasting of suspended sediment concentration using ensemble models
Abstract
This study explores two ideas to made an improvement on the artificial neural network (ANN)-based models for suspended sediment forecasting in several time steps ahead. In this regard, both observed and forecasted time series are incorporated as input variables of the models when applied for more than one lead time. Secondly, least-square ensemble models employing multiple wavelet-ANN models are developed to increase the performance of the single model. For this purpose, different wavelet families are linked with the ANN model and performance of each model is evaluated using error measures. The Skagit River near Mount Vernon in Washington county is selected as the case study. The daily flow discharge and suspended sediment concentration (SSC) in the current day are considered as input variables to predict suspended sediment concentration in the next day. For more lead times, the input structure is updated by adding the forecast of SSC in the previous time step. Results of this study demonstrate that incorporating both observed and predicted variables in the input structure improves performance of conventional models in which those only employ observed time series as input variables. Moreover, ensemble model developed for each lead time outperforms the best single wavelet-ANN model which indicates superiority of the ensemble model over the other one. Findings of this study reveal that acceptable forecasts of daily suspended sediment concentration up to 3 days in advance can be achieved using the proposed methodology.
http://ift.tt/2y9ro7E
Vitamin D and the Skin: An Update for Dermatologists
Abstract
Vitamin D plays a key role in skeletal and cardiovascular disorders, cancers, central nervous system diseases, reproductive diseases, infections, and autoimmune and dermatological disorders. The two main sources of vitamin D are sun exposure and oral intake, including vitamin D supplementation and dietary intake. Multiple factors are linked to vitamin D status, such as Fitzpatrick skin type, sex, body mass index, physical activity, alcohol intake, and vitamin D receptor polymorphisms. Patients with photosensitive disorders tend to avoid sun exposure, and this practice, along with photoprotection, can put this category of patients at risk for vitamin D deficiency. Maintaining a vitamin D serum concentration within normal levels is warranted in atopic dermatitis, psoriasis, vitiligo, polymorphous light eruption, mycosis fungoides, alopecia areata, systemic lupus erythematosus, and melanoma patients. The potential determinants of vitamin D status, as well as the benefits and risks of vitamin D (with a special focus on the skin), will be discussed in this article.
http://ift.tt/2gmQfL9
Biofilm formation by Candida albicans is inhibited by photodynamic antimicrobial chemotherapy (PACT), using chlorin e6: increase in both ROS production and membrane permeability
Abstract
Candida albicans is an opportunistic fungal producing both superficial and systemic infections in immunocompromised patients. Furthermore, it has been described an increase in the frequency of infections which have become refractory to standard antifungal therapy. Photodynamic antimicrobial chemotherapy (PACT) is a potential antimicrobial therapy that combines visible light and a nontoxic dye, known as a photosensitizer, producing reactive oxygen species (ROS) that can kill the treated cells. The objective of this study was to investigate the effects of PACT, using chlorin e6, as a photosensitizer on C. albicans. In this work, we studied the effect of PACT on both cell growth and biofilm formation by C. albicans. In addition, both ROS production and cell permeability were determined after PACT. PACT inhibited both growth and biofilm formation by C. albicans. We have also observed that PACT increased both ROS production (six times) and cell membrane permeability (five times) in C. albicans. PACT decreased both cell growth and biofilm development. The effect of PACT using chlorin e6 on C. albicans could be associated with an increase in ROS production, which could increase cell permeability, producing permanent damage to the cell membranes, leading to the cell death.
http://ift.tt/2fWgWG3
An inexact multistage fuzzy-stochastic programming for regional electric power system management constrained by environmental quality
Abstract
Electric power system involves different fields and disciplines which addressed the economic system, energy system, and environment system. Inner uncertainty of this compound system would be an inevitable problem. Therefore, an inexact multistage fuzzy-stochastic programming (IMFSP) was developed for regional electric power system management constrained by environmental quality. A model which concluded interval-parameter programming, multistage stochastic programming, and fuzzy probability distribution was built to reflect the uncertain information and dynamic variation in the case study, and the scenarios under different credibility degrees were considered. For all scenarios under consideration, corrective actions were allowed to be taken dynamically in accordance with the pre-regulated policies and the uncertainties in reality. The results suggest that the methodology is applicable to handle the uncertainty of regional electric power management systems and help the decision makers to establish an effective development plan.
http://ift.tt/2y61yzm
cBiT: A transcriptomics database for innovative biomaterial engineering
Publication date: December 2017
Source:Biomaterials, Volume 149
Author(s): Dennie G.A.J. Hebels, Aurélie Carlier, Maarten L.J. Coonen, Daniël H. Theunissen, Jan de Boer
Creating biomaterials that are suited for clinical application is still hampered by a lack of understanding of the interaction between a cell and the biomaterial surface it grows on. This surface communication can strongly impact cellular behavior, which in turn affects the chances of a successful interaction between a material and the host tissue. Transcriptomics data have previously been linked to measurements of biomaterial properties in order to explain the biological mechanisms underlying these cell-biomaterial interactions. However, such multi-assay data are highly complex and therefore require careful and unambiguous characterization and storage. Failure to do so may result in loss of valuable data or erroneous data analysis. In order to start a new initiative that tackles these issues and offers a platform for innovative biomaterial development, we have created a publically accessible repository called The Compendium for Biomaterial Transcriptomics (cBiT, http://ift.tt/2y6bAjN). cBiT is a data warehouse that gives users the opportunity to search through biomaterial-based transcriptomics data sets using a web interface. Data of interest can be selected and downloaded, together with associated measurements of material properties. Researchers are also invited to add their data to cBiT in order to further enhance its scientific value. We aim to make cBiT the hub for biomaterial-associated data, thereby enabling major contributions to a more efficient development of new materials with improved body integration. Here, we describe the structure of cBiT and provide a use case with clinically applied materials to demonstrate how cBiT can be used to correlate data across transcriptomics studies.
Graphical abstract
http://ift.tt/2y5UhiO
Neuro-Psychiatric Comorbidity among Psoriatic Adolescents
Abstract
Background
Psoriasis is a known risk factor for neuro-psychiatric diseases among adults. Less is known regarding adolescents.
Objective
Investigating the association between psoriasis and neuro-psychiatric comorbidity and social skills among adolescents.
Methods
A population based cross-sectional study between January 1st, 1999 and January 1st, 2014 was conducted. The study included 1746 and 1366 adolescents (aged 16 to 18) with mild and moderate to severe psoriasis, respectively. All psoriasis patients were diagnosed by a dermatologist. Neuro-psychiatric diseases were diagnosed by a neurologist and a psychiatrist, accordingly. Social skills were evaluated by a psychosocial assessment. Psoriasis patients were compared to 884653 healthy control patients by a multivariate analysis adjusted for age, sex, country of origin, socio-economic status, cognitive skills and body mass index. A subgroup evaluation was done for comorbidity which could only be evaluated for part of the recruitment years, by a univariate analysis.
Results
Overall chronic headaches (8.1% vs. 3.4%), intermediate frequency migraine (4.8% vs. 1.6%), low frequency migraine and non-migraine headaches (3.4% vs. 1.8%) were associated only with moderate to severe psoriasis compared to healthy controls [Adjusted odds ratios (adj. ORs) 1.9, 2.3, 1.5; 95% confidence intervals (CIs) 1.6-2.4, 1.8-3.0 and 1.1-2.1, respectively]. Anxiety disorders (2.1% vs. 0.7%) and impaired social adjustment skills (7.5% vs. 4.2%) were also associated only with moderate to severe psoriasis compared to healthy controls (ORs 2.9, 1.9; 95% CIs 1.6-5.5 and 1.3-2.6; Of 466 vs. 265023, respectively).
Conclusion
Psoriasis among adolescents is associated with neuro-psychiatric co-morbidities and impaired adjustment skills, depending on disease severity.
This article is protected by copyright. All rights reserved.
http://ift.tt/2wGhQNB
Epigenetic regulation of DNA repair genes and implications for tumor therapy
Publication date: Available online 7 October 2017
Source:Mutation Research/Reviews in Mutation Research
Author(s): Markus Christmann, Bernd Kaina
DNA repair represents the first barrier against genotoxic stress causing metabolic changes, inflammation and cancer. Besides its role in preventing cancer, DNA repair needs also to be considered during cancer treatment with radiation and DNA damaging drugs as it impacts therapy outcome. The DNA repair capacity is mainly governed by the expression level of repair genes. Alterations in the expression of repair genes can occur due to mutations in their coding or promoter region, changes in the expression of transcription factors activating or repressing these genes, and/or epigenetic factors changing histone modifications and CpG promoter methylation or demethylation levels. In this review we provide an overview on the epigenetic regulation of DNA repair genes. We summarize the mechanisms underlying CpG methylation and demethylation, with de novo methyltransferases and DNA repair involved in gain and loss of CpG methylation, respectively. We discuss the role of components of the DNA damage response, p53, PARP-1 and GADD45a on the regulation of the DNA (cytosine-5)-methyltransferase DNMT1, the key enzyme responsible for gene silencing. We stress the relevance of epigenetic silencing of DNA repair genes for tumor formation and tumor therapy. A paradigmatic example is provided by the DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT), which is silenced in up to 40% of various cancers through CpG promoter methylation. The CpG methylation status of the MGMT promoter strongly correlates with clinical outcome and, therefore, is used as prognostic marker during glioblastoma therapy. Mismatch repair genes are also subject of epigenetic silencing, which was shown to correlate with colorectal cancer formation. For many other repair genes shown to be epigenetically regulated the clinical outcome is not yet clear. We also address the question of whether genotoxic stress itself can lead to epigenetic alterations of genes encoding proteins involved in the defense against genotoxic stress.
http://ift.tt/2y2bsnL
Fast in situ gamma spectroscopy using hand-held spectrometer with NaI probe
Source:Journal of Environmental Radioactivity
Author(s): Szymon Guguła, Krzysztof Kozak, Jadwiga Mazur, Dominik Grządziel, Mariusz Mroczek
In this work a hand-held spectrometer InSpector 1000 with NaI (Tl) 2″ x 2″ detector has been adapted to fast in situ gamma-ray spectroscopy. Two specially designed mounting stands with shielding have been built, allowing conducting measurements in different geometries. Three particular geometries (NW, IS50, IS00) have been chosen for efficiency calibration and further study. The first one (NW) is intended for small environmental samples (volume ca 140 cm3) collected on site. IS50 geometry is a typical in situ geometry meant for radioactivity measurements in soil with detector pointed towards the ground. In this geometry the probe is shielded and mounted 50 cm above the soil surface. The new proposed geometry IS00 is designed in the way that the detector is inserted directly into the soil in order to increase the counting efficiency. The methods of efficiency calibration involved using calibration standards (in NW geometry) and the results obtained in previous in situ measurements with InSpector 2000 portable spectrometer with HPGe detector and ISOCS™ Shield Systems, which is routinely used in environmental measurements.NW geometry turned out to be useful for natural radioisotopes concentrations (K-40, U-238 and Th-232), which significantly exceed typical values of those concentrations observed in Poland. Both IS50 and IS00 geometries are applicative for quick (2 h long measurement) evaluation of typical concentrations of K, U and Th in soils. The newly proposed geometry IS00 is superior as it showed lower detection limits and uncertainties as well as its handling was far easier than of IS50. Authors have proven that hand-held spectrometer InSpector 1000, together with mounting stands and shielding, can be successfully used for fast in situ gamma-spectroscopy. Its relatively small weight and good mobility are additional assets. Moreover, detailed procedures for measurements in each geometry have been developed to conduct such analyses properly.
http://ift.tt/2xs2agy
Ponatinib in chronic myeloid leukemia (CML): Consensus on patient treatment and management from a European expert panel
Source:Critical Reviews in Oncology/Hematology
Author(s): Martin C. Müller, Francisco Cervantes, Henrik Hjorth-Hansen, Jeroen J.W.M. Janssen, Dragana Milojkovic, Delphine Rea, Gianantonio Rosti
Five tyrosine kinase inhibitors (TKIs) are currently approved in the European Union for treatment of chronic myeloid leukemia (CML) and all have considerable overlap in their indications. While disease-specific factors such as CML phase, mutational status, and line of treatment are key to TKI selection, other important features must be considered, such as patient-specific comorbidities and TKI safety profiles. Ponatinib, the TKI most recently approved, has demonstrated efficacy in patients with refractory CML, but is associated with an increased risk of arterial hypertension, sometimes severe, and serious arterial occlusive and venous thromboembolic events. A panel of European experts convened to discuss their clinical experience in managing patients with CML. Based on the panel discussions, scenarios in which a CML patient may be an appropriate candidate for ponatinib therapy are described, including presence of the T315I mutation, resistance to other TKIs without the T315I mutation, and intolerance to other TKIs.
http://ift.tt/2z6Cqsh
MicroRNAs in glioblastoma pathogenesis and therapy: A comprehensive review
Source:Critical Reviews in Oncology/Hematology
Author(s): Bhavesh K. Ahir, Howard Ozer, Herbert H. Engelhard, Sajani S. Lakka
Glioblastoma (GBM), also known as grade IV astrocytoma, is the most aggressive primary intracranial tumor of the adult brain. MicroRNAs (miRNAs), a class of small non-coding RNA species, have critical functions across various biological processes. A great deal of progress has been made recently in dissecting miRNA pathways associated with the pathogenesis of GBM. miRNA expression signatures called gene signatures also characterize and contribute to the phenotypic diversity of GBM subclasses through their ability to regulate developmental growth and differentiation. miRNA molecules have been identified as diagnostic and prognostic biomarkers for patient stratification and may also serve as therapeutic targets and agents. This review summarizes: (i) the current understanding of the roles of miRNAs in the pathogenesis of GBM, (ii) the potential use of miRNAs in GBM diagnosis and glioma grading, (iii) further prospects of developing miRNAs as novel biomarkers and therapeutic targets for GBM, and (iv) important practical considerations when considering miRNA therapy for GBM patients.
http://ift.tt/2y9J5DG
Comparison of gamma- and DVH-based in vivo dosimetric plan evaluation for pelvic VMAT treatments
Source:Radiotherapy and Oncology
Author(s): Erik van der Bijl, René F.M. van Oers, Igor Olaciregui-Ruiz, Anton Mans
Background and purposeTo compare DVH-based quality assurance to a multi-parametric γ-based methodology for in vivo EPID dosimetry for VMAT to the pelvis.Materials and methodsFor 47 rectum, 37 prostate, and 44 bladder VMAT treatments we reconstructed the 3D dose distributions of 387 fractions from in vivo EPID dosimetry. The difference between planned and measured dose was evaluated using γ analysis (3%/3mm) in the 50% isodose volume (IDV) and DVH differences (ΔD2, ΔD50 and ΔD98) of targets and organs at risk. The γ-indicators mean γ, γ pass rate and γ1% were compared to DVH-differences and their correlations were studied. DVH-based alerts on PTV and IDV were compared to γ-based alerts.ResultsAverage PTV D50 and D98 dose differences were 0.0±2.2% (1SD) and −1.4±2.9% (1SD). Alert criteria of |ΔD50|<3.5–4.5% corresponded to an alert rate of about 10%. Strong correlations between mean γ and γ pass rate and difference in PTV ΔD50 were observed for all sites. DVH- and γ-based alerts agreed on >80% of the fractions for the majority of compared alert thresholds and methods. This agreement is >90% for the larger deviations.ConclusionsStrong correlations between some γ- and DVH indicators were found. Our comparison of multi-parametric alert strategies showed clinical equivalence for γ- and DVH-based methods.
http://ift.tt/2y7OTOg
Thyroid hormone levels in late preterm, early term and term infants: a study with healthy neonates revealing reference values and factors affecting thyroid hormones
Journal Name: Journal of Pediatric Endocrinology and Metabolism
Issue: Ahead of print
http://ift.tt/2y5ReY6
Severe complications after initial management of hyperglycemic hyperosmolar syndrome and diabetic ketoacidosis with a standard diabetic ketoacidosis protocol
Journal Name: Journal of Pediatric Endocrinology and Metabolism
Issue: Ahead of print
http://ift.tt/2yz2nUc
The relationship between non-alcoholic fatty liver disease and small intestinal bacterial overgrowth among overweight and obese children and adolescents
Journal Name: Journal of Pediatric Endocrinology and Metabolism
Issue: Ahead of print
http://ift.tt/2y4GR6N
Persistent de Quervain tenosynovitis induced by somatotropin treatment
Journal Name: Journal of Pediatric Endocrinology and Metabolism
Issue: Ahead of print
http://ift.tt/2wFLPp5
Oral health status of children with type 1 diabetes: a comparative study
Journal Name: Journal of Pediatric Endocrinology and Metabolism
Issue: Ahead of print
http://ift.tt/2kxDCkZ
Makorin ring finger 3 gene analysis in Koreans with familial precocious puberty
Journal Name: Journal of Pediatric Endocrinology and Metabolism
Issue: Ahead of print
http://ift.tt/2wGwYuD
Emerging targets in cancer immunotherapy
Source:Seminars in Cancer Biology
Author(s): Samantha Burugu, Amanda R. Dancsok, Torsten O. Nielsen
The first generation of immune checkpoint inhibitors (anti-CTLA-4 and anti-PD-1/PD-L1) targeted natural immune homeostasis pathways, co-opted by cancers, to drive anti-tumor immune responses. These agents led to unprecedented results in patients with previously incurable metastatic disease and may become first-line therapies for some advanced cancers. However, these agents are efficacious in only a minority of patients. Newer strategies are becoming available that target additional immunomodulatory mechanisms to activate patients' own anti-tumor immune responses. Herein, we present a succinct summary of emerging immune targets with reported pre-clinical efficacy that have progressed to active investigation in clinical trials. These emerging targets include co-inhibitory and co-stimulatory markers of the innate and adaptive immune system. In this review, we discuss: 1) T lymphocyte markers: Lymphocyte Activation Gene 3 [LAG-3], T-cell Immunoglobulin- and Mucin-domain-containing molecule 3 [TIM-3], V-domain containing Ig Suppressor of T cell Activation [VISTA], T cell ImmunoGlobulin and ITIM domain [TIGIT], B7-H3, Inducible T-cell Co-stimulator [ICOS/ICOS-L], CD27/CD70, and Glucocorticoid-Induced TNF Receptor [GITR]; 2) macrophage markers: CD47/Signal-Regulatory Protein alpha [SIRPα] and Indoleamine-2,3-Dioxygenase [IDO]; and 3) natural killer cell markers: CD94/NKG2A and the Killer Immunoglobulin-like receptor [KIR] family. Finally, we briefly highlight combination strategies and potential biomarkers of response and resistance to these cancer immunotherapies.
http://ift.tt/2wFHrq3
A new technique for adult hypospadias repair: A combination of TIPU and Mathieu techniques
Publication date: Available online 7 October 2017
Source:The Kaohsiung Journal of Medical Sciences
Author(s): Murat Zor, Hasret Topac, Onder Ors
http://ift.tt/2fUbV0D
Hybrid polymer films based ZnS nanocomposites and its optical and morphological properties: Monitoring the role of the binding-site interaction
Publication date: February 2018
Source:Materials Research Bulletin, Volume 98
Author(s): Guadalupe del C. Pizarro, Oscar G. Marambio, Manuel Jeria-Orell, Diego P. Oyarzún, Julio Sánchez
A series of hybrid polymer films were prepared using self-assembly block copolymers as nanoreactor of ZnS nanoparticles (NPs). The self-assembled poly(acrylic acid)-block-poly(N-phenylmaleimide) was prepared by Atom-Transfer Radical Polymerization (ATRP) in different block composition. Subsequently the fluorescent NPs were synthesized into the polymer matrices in order to modify its optical and thermal properties and to analyze its effect on the morphology properties (roughness and porosity).As results, significant effects on the properties of the hybrid materials were obtained under different percentages of the NPs. These materials exhibited blue- or red-shift emission bands depending on the length of hydrophilic domain. Additionally, a quenched emission band is seen, which may also be attributed primarily to the composition of the hydrophilic domain and to the greater percentage of ZnS NPs. The hybrid polymer films showed effects on the porosity and roughness of the surfaces, which was analyzed by AFM and SEM microscopic techniques.
Graphical abstract
http://ift.tt/2xrWZ07
Eyes have ears: Indexing the orienting response to sound using pupillometry
Source:International Journal of Psychophysiology
Author(s): Alexandre Marois, Katherine Labonté, Mark Parent, François Vachon
The rare occurrence of a sound deviating from the auditory background tends to trigger attentional orienting. While some sympathetic physiological responses can be used to index this orienting response, findings surrounding the pupillary dilation response (PDR) as a proxy for the orienting response are conflicting. The current study was tailor-designed to examine whether the PDR satisfies specific criteria of an orienting response index, namely the classic habituation pattern and a sensitivity to the size of the deviation. The PDR decrement to a repeated standard sound, recovery to a deviant sound, and dishabituation to the re-presentation of the standard were assessed for small and large deviations embedded in irrelevant auditory sequences. The PDR not only showed habituation and dishabituation, but also recovered in correspondence with the magnitude of the acoustic deviation. This consistency between variations of the PDR and orienting response's properties indicates that the PDR is a valid index of the auditory orienting response.
http://ift.tt/2g2zubw
Using theta and alpha band power to assess cognitive workload in multitasking environments
Source:International Journal of Psychophysiology
Author(s): Sébastien Puma, Nadine Matton, Pierre-V. Paubel, Éric Raufaste, Radouane El-Yagoubi
Cognitive workload is of central importance in the fields of human factors and ergonomics. A reliable measurement of cognitive workload could allow for improvements in human machine interface designs and increase safety in several domains. At present, numerous studies have used electroencephalography (EEG) to assess cognitive workload, reporting the rise in cognitive workload to be associated with increases in theta band power and decreases in alpha band power. However, results have been inconsistent with some failing to reach the required level of significance. We hypothesized that the lack of consistency could be related to individual differences in task performance and/or to the small sample sizes in most EEG studies. In the present study we used EEG to assess the increase in cognitive workload occurring in a multitasking environment while taking into account differences in performance. Twenty participants completed a task commonly used in airline pilot recruitment, which included an increasing number of concurrent sub-tasks to be processed from one to four. Subjective ratings, performances scores, pupil size and EEG signals were recorded. Results showed that increases in EEG alpha and theta band power reflected increases in the involvement of cognitive resources for the completion of one to three subtasks in a multitasking environment. These values reached a ceiling when performances dropped. Consistent differences in levels of alpha and theta band power were associated to levels of task performance: highest performance was related to lowest band power.
http://ift.tt/2wFw3KH
Development of a microarray-based assay for efficient testing of new HSP70/DnaK inhibitors
Publication date: Available online 7 October 2017
Source:Bioorganic & Medicinal Chemistry
Author(s): Sona Mohammadi-Ostad-Kalayeh, Vjaceslavs Hrupins, Sabine Helmsen, Frank Stahl, Thomas Scheper, Matthias Preller, Frank Surup, Marc Stadler, Andreas Kirschning, Carsten Zeilinger
A facile method for testing ATP binding in a highly miniaturized microarray environment using human HSP70 and DnaK from Mycobacterium tuberculosis as biological targets is reported. Supported by molecular modelling studies we demonstrate that the position of the fluorescence label on ATP has a strong influence on the binding to human HSP70. Importantly, the label has to be positioned on the adenine ring and not to the terminal phosphate group. Unlabelled ATP displaced bound Cy5-ATP from HSP70 in the micromolar range. The affinity of a well-known HSP70 inhibitor VER155008 for the ATP binding site in HSP70 was determined, with a EC50 in the micromolar range, whereas reblastin, a HSP90-inhibitor, did not compete for ATP in the presence of HSP70. The applicability of the method was demonstrated by screening a small compound library of natural products. This unraveled that terphenyls rickenyl A and D, recently isolated from cultures of the fungus Hypoxylon rickii, are inhibitors of HSP70. They compete with ATP for the chaperone in the range of 29 µM (Rickenyl D) and 49 µM (Rickenyl A). Furthermore, the microarray-based test system enabled protein-protein interaction analysis using full-length HSP70 and HSP90 proteins. The labelled full-length human HSP90 binds with a half-maximal affinity of 5.5 µg/ml (∼40 µM) to HSP70. The data also demonstrate that the microarray test has potency for many applications from inhibitor screening to target-oriented interaction studies.
Graphical abstract
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The burden of atopic dermatitis in US adults: Health care resource utilization data from the 2013 National Health and Wellness Survey
Publication date: Available online 7 October 2017
Source:Journal of the American Academy of Dermatology
Author(s): Laurent Eckert, Shaloo Gupta, Caroline Amand, Abhijit Gadkari, Puneet Mahajan, Joel M. Gelfand
BackgroundThere is a lack of data on the burden of atopic dermatitis (AD) in adults relative to the general population.ObjectiveTo characterize the AD burden in adult patients relative to both matched non-AD controls and matched patients with psoriasis in terms of comorbidities, health care resource utilization (HCRU), and costs.MethodsAdults (≥18 years) who self-reported a diagnosis of AD or psoriasis and adult non-AD controls were identified from the 2013 US National Health and Wellness Survey. Patients with AD were propensity score–matched with non-AD controls and patients with psoriasis on demographic variables. Patient-reported outcomes were analyzed between matched cohorts.ResultsPatients with AD had a significantly greater risk for atopic comorbidities, as well as significantly greater HCRU and total cost compared with non-AD controls. The burden of AD was generally comparable to that of psoriasis, although patients with AD reported increased use of emergency room visits compared with patients with psoriasis.LimitationsPatient-reported data are susceptible to recall bias and erroneous classification.ConclusionsAdult patients with AD reported a substantial disease burden, suggesting an unmet need for more effective AD treatment options.
http://ift.tt/2xqI3iK
The Frisbee Maneuver: A Novel Method to Abort Acute Attacks of Raynaud’s Phenomenon
Publication date: Available online 7 October 2017
Source:Journal of the American Academy of Dermatology
Author(s): Paul Curtiss, Gabriela Cobos, Kristen Lo Sicco, Andrew Franks
http://ift.tt/2yzaUq4
Focal Atrichia: A Diagnostic Clue in Female Pattern Hair Loss
Publication date: Available online 7 October 2017
Source:Journal of the American Academy of Dermatology
Author(s): Elise A. Olsen, David A. Whiting
BackgroundFocal atrichia is a common clinical finding in female pattern hair loss whose specificity and histologic findings need further clarification.ObjectiveTo determine the frequency of focal atrichia in various types of hair loss and its histologic characteristics in female pattern hair loss.MethodsPart 1: Review of 250 consecutive female patients seen with hair loss for the presence of focal atrichia and Part 2: paired biopsies of haired areas vs focal atrichia in 18 subjects with female pattern hair lossResultsFocal atrichia was seen in 46/104 (44%) of women with female pattern hair loss, including 67% of late onset vs 15% of early onset, compared to 3/146 (2%) of those with other hair disorders Biopsy findings of focal atrichia in female pattern hair loss showed primarily a more progressive miniaturization process than that of haired areas of the scalp.LimitationsSome women with female pattern hair loss may have had concomitant chronic telogen effluviumConclusionsWhen present, focal atrichia is a clinical clue to the diagnosis of female pattern hair loss, particularly late onset subtype.
http://ift.tt/2xr5t7M
Alternating histopathological pattern in blaschkoid dermatoses with epidermal changes: A retrospective series of 61 cases
Publication date: Available online 7 October 2017
Source:Journal of the American Academy of Dermatology
Author(s): Vishal Gupta, Sujay Khandpur, Sudheer Arava, M. Ramam
http://ift.tt/2yzaW1a
QTc prolongation during phenol-croton oil peels
Publication date: Available online 7 October 2017
Source:Journal of the American Academy of Dermatology
Author(s): Carlos Gustavo Wambier, Sarah Perillo de Farias Wambier, Liliana Elias Pena Pilatti, Jessica Adriana Grabicoski, Luis Fernando Wambier, André Schmidt
http://ift.tt/2yzaTT2
Structural requirements of flavonoids to induce heme oxygenase-1 expression
Publication date: December 2017
Source:Free Radical Biology and Medicine, Volume 113
Author(s): K.D. Croft, D. Zhang, R. Jiang, A. Ayer, S. Shengule, R.J. Payne, N.C. Ward, R. Stocker
Population studies suggest cardiovascular health benefits of consuming fruits and vegetables rich in polyphenolic compounds such as flavonoids. We reported previously that the flavonoid quercetin protects arteries from oxidant-induced endothelial dysfunction and attenuates atherosclerosis in apolipoprotein E gene knockout mice, with induction of heme oxygenase-1 (Hmox1) playing a critical role. The present study investigated the structural requirements of flavonoids to induce Hmox1 in human aortic endothelial cells (HAEC). We identified ortho-dihydroxyl groups and an α,β-unsaturated system attached to a catechol as the key structural requirements for Hmox1 induction. Active but not inactive flavonoids had a low oxidation potential and prevented ascorbate autoxidation, suggesting that Hmox1 inducers readily undergo oxidation and that oxidized, rather than reduced, flavonoids may be the biological inducer of Hmox1. To test this hypothesis, we synthesized stable derivatives of caffeic acid (3-(3,4-dihyroxyphenyl)-2-propenoic acid) containing either ortho-dihydroxy or ortho-dioxo groups. Compared with the dihydroxy compound, the quinone analog induced Hmox1 more potently in HAEC and also provided enhanced protection to arteries of wild type animals against oxidant-induced endothelial dysfunction. In contrast, the quinone analog failed to provide protection against oxidant-induced endothelial dysfunction in arteries of Hmox1–/– mice, establishing a key role for Hmox1 in vascular protection. These results suggest that oxidized forms of dietary polyphenols are the likely inducers of Hmox1 and may explain in part the protective cardiovascular effects of diets rich in these compounds.
Graphical abstract
http://ift.tt/2fUhzQB
UCP2 inhibition induces ROS/Akt/mTOR axis: Role of GAPDH nuclear translocation in genipin/everolimus anticancer synergism
Publication date: December 2017
Source:Free Radical Biology and Medicine, Volume 113
Author(s): Ilaria Dando, Raffaella Pacchiana, Elisa Dalla Pozza, Ivana Cataldo, Stefano Bruno, Paola Conti, Marco Cordani, Anna Grimaldi, Giovanna Butera, Michele Caraglia, Aldo Scarpa, Marta Palmieri, Massimo Donadelli
Several studies indicate that mitochondrial uncoupling protein 2 (UCP2) plays a pivotal role in cancer development by decreasing reactive oxygen species (ROS) produced by mitochondrial metabolism and by sustaining chemoresistance to a plethora of anticancer drugs. Here, we demonstrate that inhibition of UCP2 triggers Akt/mTOR pathway in a ROS-dependent mechanism in pancreatic adenocarcinoma cells. This event reduces the antiproliferative outcome of UCP2 inhibition by genipin, creating the conditions for the synergistic counteraction of cancer cell growth with the mTOR inhibitor everolimus. Inhibition of pancreatic adenocarcinoma cell growth and induction of apoptosis by genipin and everolimus treatment are functionally related to nuclear translocation of the cytosolic glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH). The synthetic compound (S)-benzyl-2-amino-2-(S)-3-bromo-4,5-dihydroisoxazol-5-yl-acetate (AXP3009), which binds GAPDH at its redox-sensitive Cys152, restores cell viability affected by the combined treatment with genipin and everolimus, suggesting a role for ROS production in the nuclear translocation of GAPDH. Caspase-mediated apoptosis by genipin and everolimus is further potentiated by the autophagy inhibitor 3-methyladenine revealing a protective role for Beclin1-mediated autophagy induced by the treatment. Mice xenograft of pancreatic adenocarcinoma further confirmed the antiproliferative outcome of drug combination without toxic effects for animals. Tumor masses from mice injected with UCP2 and mTOR inhibitors revealed a strong reduction in tumor volume and number of mitosis associated with a marked GAPDH nuclear positivity. Altogether, these results reveal novel mechanisms through which UCP2 promotes cancer cell proliferation and support the combined inhibition of UCP2 and of Akt/mTOR pathway as a novel therapeutic strategy in the treatment of pancreatic adenocarcinoma.
http://ift.tt/2fVVD7J
Redox imbalance due to the loss of mitochondrial NAD(P)-transhydrogenase markedly aggravates high fat diet-induced fatty liver disease in mice
Publication date: December 2017
Source:Free Radical Biology and Medicine, Volume 113
Author(s): Claudia D.C. Navarro, Tiago R. Figueira, Annelise Francisco, Genoefa A. Dal'Bó, Juliana A. Ronchi, Juliana C. Rovani, Cecilia A.F. Escanhoela, Helena C.F. Oliveira, Roger F. Castilho, Anibal E. Vercesi
The mechanisms by which a high fat diet (HFD) promotes non-alcoholic fatty liver disease (NAFLD) appear to involve liver mitochondrial dysfunctions and redox imbalance. We hypothesized that a HFD would increase mitochondrial reliance on NAD(P)-transhydrogenase (NNT) as the source of NADPH for antioxidant systems that counteract NAFLD development. Therefore, we studied HFD-induced liver mitochondrial dysfunctions and NAFLD in C57Unib.B6 congenic mice with (Nnt+/+) or without (Nnt-/-) NNT activity; the spontaneously mutated allele (Nnt-/-) was inherited from the C57BL/6J mouse substrain. After 20 weeks on a HFD, Nnt-/- mice exhibited a higher prevalence of steatohepatitis and content of liver triglycerides compared to Nnt+/+ mice on an identical diet. Under a HFD, the aggravated NAFLD phenotype in the Nnt-/- mice was accompanied by an increased H2O2 release rate from mitochondria, decreased aconitase activity (a redox-sensitive mitochondrial enzyme) and higher susceptibility to Ca2+-induced mitochondrial permeability transition. In addition, HFD led to the phosphorylation (inhibition) of pyruvate dehydrogenase (PDH) and markedly reduced the ability of liver mitochondria to remove peroxide in Nnt-/- mice. Bypass or pharmacological reactivation of PDH by dichloroacetate restored the peroxide removal capability of mitochondria from Nnt-/- mice on a HFD. Noteworthy, compared to mice that were chow-fed, the HFD did not impair peroxide removal nor elicit redox imbalance in mitochondria from Nnt+/+ mice. Therefore, HFD interacted with Nnt mutation to generate PDH inhibition and further suppression of peroxide removal. We conclude that NNT plays a critical role in counteracting mitochondrial redox imbalance, PDH inhibition and advancement of NAFLD in mice fed a HFD. The present study provide seminal experimental evidence that redox imbalance in liver mitochondria potentiates the progression from simple steatosis to steatohepatitis following a HFD.
Graphical abstract
http://ift.tt/2y8Ekuu
Modulating microfibrillar alignment and growth factor stimulation to regulate mesenchymal stem cell differentiation
Publication date: Available online 7 October 2017
Source:Acta Biomaterialia
Author(s): Dinorath Olvera, Binulal N. Sathy, Simon F. Carroll, Daniel J. Kelly
The ideal tissue engineering (TE) strategy for ligament regeneration should recapitulate the bone – calcified cartilage – fibrocartilage – soft tissue interface. Aligned electrospun-fibers have been shown to guide the deposition of a highly organized extracellular matrix (ECM) necessary for ligament TE. However, recapitulating the different tissues observed in the bone-ligament interface using such constructs remains a challenge. This study aimed to explore how fiber alignment and growth factor stimulation interact to regulate the chondrogenic and ligamentous differentiation of mesenchymal stem cells (MSCs). To this end aligned and randomly-aligned electrospun microfibrillar scaffolds were seeded with bone marrow derived MSCs and stimulated with transforming growth factor β3 (TGFβ3) or connective tissue growth factor (CTGF), either individually or sequentially. Without growth factor stimulation, MSCs on aligned-microfibers showed higher levels of tenomodulin (TNMD) and aggrecan gene expression compared to MSCs on randomly-oriented fibers. MSCs on aligned-microfibers stimulated with TGFβ3 formed cellular aggregates and underwent robust chondrogenesis, evidenced by increased type II collagen expression and sulphated glycosaminoglycans (sGAG) synthesis compared to MSCs on randomly-oriented scaffolds. Bone morphogenetic protein 2 (BMP2) and type I collagen gene expression were higher on randomly-oriented scaffolds stimulated with TGFβ3, suggesting this substrate was more supportive of an endochondral phenotype. In the presence of CTGF, MSCs underwent ligamentous differentiation, with increased TNMD expression on aligned compared to randomly aligned scaffolds. Upon sequential growth factor stimulation, MSCs expressed types I and II collagen and deposited higher overall levels of collagen compared to scaffolds stimulated with either growth factor in isolation. These findings demonstrate that modulating the alignment of microfibrillar scaffolds can be used to promote either an endochondral, chondrogenic, fibro-chondrogenic or ligamentous MSC phenotype upon presentation of appropriate biochemical cues.Statement of significancePolymeric electrospun fibers can be tuned to match the fibrillar size and anisotropy of collagen fibers in ligaments, and can be mechanically competent. Therefore, their use is attractive when attempting to tissue engineer the bone-ligament interface. A central challenge in this field is recapitulating the cellular phenotypes observed across the bone-ligament interface. Here we demonstrated that it is possible to direct MSCs seeded onto aligned electrospun fibres towards either a ligamentogenic, chondrogenic or fibrochondrogenic phenotype upon presentation of appropriate biochemical cues. This opens the possibility of using aligned microfibrillar scaffolds that are spatially functionalized with specific growth factors to direct MSC differentiation for engineering the bone-ligament interface.
Graphical abstract
http://ift.tt/2y8CYj2
A comprehensive review of non-enterica subspecies of Salmonella enterica
Publication date: Available online 7 October 2017
Source:Microbiological Research
Author(s): Alexandre Lamas, José Manuel Miranda, Patricia Regal, Beatriz Vázquez, Carlos Manuel Franco, Alberto Cepeda
Salmonella is a major foodborne pathogen with a complex nomenclature. This genus is composed of two species, S. enterica and S. bongori. S. enterica is divided into six subspecies. S. enterica subspecies enterica is composed of more than 1,500 serotypes with some of great importance, such as S. Typhimurium and S. Enteritidis. S. enterica subsp. enterica is responsible of more than 99% of human salmonellosis and therefore it is widely studied. However, the non-enterica subspecies of S. enterica have been little studied. These subspecies are considered to be related to cold-blooded animals and their pathogenicity is very limited. Phenotype and genotype information generated from different studies of non-enterica subspecies reveal poor ability to invade host cells and the absence or modification of important virulence factors. Also, the great majority of human infections due to non-enterica subspecies are related to a previous depressed immune system. Therefore, we propose to treat these subspecies only as opportunistic pathogens. For establish this premise, the present review evaluated, among other things, the genomic characteristics, prevalence, antimicrobial resistance and reported human cases of the non-enterica subspecies.
http://ift.tt/2xpqQLw
Automated segmentation of abdominal subcutaneous adipose tissue and visceral adipose tissue in obese adolescent in MRI
Source:Magnetic Resonance Imaging
Author(s): Steve C.N. Hui, Teng Zhang, Lin Shi, Defeng Wang, Chei-Bing Ip, Winnie C.W. Chu
PurposeTo develop a reliable and reproducible automatic technique to segment and measure SAT and VAT based on MRI.Materials and methodsChemical-shift water-fat MRI were taken on twelve obese adolescents (mean age: 16.1±0.6, BMI: 31.3±2.3) recruited under the health monitoring program. The segmentation applied a spoke template created using Midpoint Circle algorithm followed by Bresenham's Line algorithm to detect narrow connecting regions between subcutaneous and visceral adipose tissues. Upon satisfaction of given constrains, a cut was performed to separate SAT and VAT. Bone marrow was consisted in pelvis and femur. By using the intensity difference in T2*, a mask was created to extract bone marrow adipose tissue (MAT) from VAT. Validation was performed using a semi-automatic method. Pearson coefficient, Bland-Altman plot and intra-class coefficient (ICC) were applied to measure accuracy and reproducibility.ResultsPearson coefficient indicated that results from the proposed method achieved high correlation with the semi-automatic method. Bland-Altman plot and ICC showed good agreement between the two methods. Lowest ICC was obtained in VAT segmentation at lower regions of the abdomen while the rests were all above 0.80. ICC (0.98–0.99) also indicated the proposed method performed good reproducibility.ConclusionNo user interaction was required during execution of the algorithm and the segmented images and volume results were given as output. This technique utilized the feature in the regions connecting subcutaneous and visceral fat and T2* intensity difference in bone marrow to achieve volumetric measurement of various types of adipose tissue in abdominal site.
http://ift.tt/2z6uAyN
An Augmented Reality Magic Mirror as Additive Teaching Device for Gross Anatomy
Source:Annals of Anatomy - Anatomischer Anzeiger
Author(s): Daniela Kugelmann, Leonard Stratmann, Nils Nühlen, Felix Bork, Saskia Hoffmann, Golbarg Samarbarksh, Anna Pferschy, Anna Maria von der Heide, Andreas Eimannsberger, Pascal Fallavollita, Nassir Navab, Jens Waschke
When preparing young medical students for clinical activity, it is indispensable to acquaint them with anatomical section images which enable them to use the clinical application of imaging methods A new Augmented Reality Magic Mirror (AR MM) system, which provides the advantage of a novel, interactive learning tool in addition to a regular dissection course, was therefore tested and evaluated by 880 first-year medical students as part of the macroscopic anatomy course in 2015/16 at Ludwig-Maximilians-Universität (LMU) in Munich. The system consists of an RGB-D sensor as a real-time tracking device, which enables the system to link a deposited section image to the projection of the user's body, as well as a large display mimicking a real-world physical mirror. Using gesture input, the users have the ability to interactively explore radiological images in different anatomical intersection planes. We designed a tutorial during which students worked with the system in groups of about 12 and evaluated the results. Subsequently, each participant was asked to assess the system's value by filling out a Likert-scale questionnaire. The respondents approved all statements which stressed the potential of the system to serve as an additional learning resource for anatomical education. In this case, emphasis was put on active learning, 3-dimensional understanding, and a better comprehension of the course of structures. We are convinced that such an AR MM system can be beneficially installed into anatomical education in order to prepare medical students more effectively for the clinical standards and for more interactive, student-centered learning.
http://ift.tt/2y2hjcE
A preliminary study to design a floating treatment wetland for remediating acid mine drainage-impacted water using vetiver grass ( Chrysopogon zizanioides )
Abstract
Acid mine drainage (AMD) is extremely acidic, sulfate-rich effluent from abandoned or active mine sites that also contain elevated levels of heavy metals. Untreated AMD can contaminate surface and groundwater and pose severe ecological risk. Both active and passive methods have been developed for AMD treatment consisting of abiotic and biological techniques. Abiotic techniques are expensive and can create large amounts of secondary wastes. Passive biological treatment mainly consists of aerobic or anaerobic constructed wetlands. While aerobic wetlands are economical, they are not effective if the pH of the AMD is < 5. Anaerobic wetlands use organic-rich substrates to provide carbon source to iron- and sulfate-reducing bacteria. The efficiency of these systems declines overtime and requires continuous maintenance. Our objective is to develop an alternative, low-cost, and sustainable floating wetland treatment (FWT) system for AMD for the abandoned Tab-Simco coal mining site in Illinois using vetiver grass (Chrysopogon zizanioides). Tab-Simco AMD is highly acidic, with mean pH value of 2.64, and contains high levels of sulfate and metals. A greenhouse study was performed for a 30-day period in order to screen and optimize the necessary parameters to design a FWT system. Water quality and plant growth parameters were continuously monitored. Results show significant SO42− removal, resulting in increased pH, particularly at higher planting densities. Vetiver also helped in metal removal; high amounts of Fe, Zn, and Cu were removed, with relatively lower amounts of Pb, Al, and Ni. Iron plaque formation on the root was observed, which increased metal stabilization in root and lowered root to shoot metal translocation. Vetiver was tolerant of AMD, showing minimal change in biomass and plant growth. Results obtained are encouraging, and a large scale mesocosm study is now in progress, as the next step to develop the vetiver-based system for AMD treatment.
http://ift.tt/2xqypg1
Organic horizon and mineral soil mercury along three clear-cut forest chronosequences across the northeastern USA
Abstract
Mercury (Hg) is a globally distributed pollutant trace metal that has been increasing in terrestrial environments due to rising anthropogenic emissions. Vegetation plays an important role in Hg sequestration in forested environments, but increasing tree removal for biofuels and wood products may affect this process. The long-term effect of clear-cutting on forest soil Hg remains uncertain, since most studies are limited to measuring changes for < 10 years following a single harvest event. The chronosequence approach, which substitutes space for time using forest stands of different ages since clear-cutting, allows for investigation of processes occurring over decades to centuries. Here, we utilized three clear-cut forest soil chronosequences across the northeastern USA to understand Hg accumulation and retention over several decades. Total Hg concentrations and pools were quantified for five soil depth increments along three chronosequences. Our results showed Hg concentrations and pools decreased in the initial 20 years following clear-cutting. Mineral soil Hg pools decreased 21–53% (7–14 mg m−2) between 1–5-year-old stands and 15–25-year-old stands but mineral soil Hg pools recovered in 55–140-year-old stands to similar values as measured in 1–5-year-old stands. Our study is one of the first to demonstrate a decrease and recovery in Hg pool size. These changes in Hg did not correspond with changes in bulk density, soil C, or pH. We utilized a simple two-box model to determine how different Hg fluxes affected organic and mineral soil horizon Hg pools. Our simple model suggests that changes in litterfall and volatilization rates could have caused the observed changes in organic horizon Hg pools. However, only increases in leaching could reproduce observed decreases to mineral soil Hg pools. Further studies are needed to determine the mechanism of Hg loss from forest soils following clear-cutting.
http://ift.tt/2wFhGpZ
Turning an environmental problem into an opportunity: potential use of biochar derived from a harmful marine biomass named Cladophora glomerata as anode electrode for Li-ion batteries
Abstract
The electrochemical performance of lithium ion battery was enhanced by using biochar derived from Cladophora glomerata (C. glomerata) as widespread green macroalgae in most areas of the Iran's Caspian sea coast. By the utilization of the structure of the biochar, micro-/macro-ordered porous carbon with olive-shaped structure was successfully achieved through pyrolysis at 500 °C, which is the optimal temperature for biofuel production, and was activated with HCl. The biochar and HCl treatment biochar (HTB) were applied as anode electrode in lithium ion batteries. Then, electrochemical measurements were conducted on the electrodes via galvanostatic charge–discharge, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS) analyses. The electrochemical results indicated a higher specific discharge capacity (700 mAh g−1) and good cycling stability for HTB at the current density of 0.1 A g−1 as compared to the biochar. The reason that HTB electrode works better than the biochar could be due to the higher surface area, formation functional groups, removal impurities, and formation some micropores after HCl treatment. The biochar derived from marine biomass and treatment process developed here could provide a promising path for the low-cost, renewable, and environmentally friendly electrode materials.
Graphical abstract
Algal-biochar into Li-ion Battery
http://ift.tt/2xqOJNX
Pitfalls and errors in measuring jitter
Publication date: November 2017
Source:Clinical Neurophysiology, Volume 128, Issue 11
Author(s): Erik Stålberg, Donald B. Sanders, João Aris Kouyoumdjian
The safety factor of neuromuscular transmission can be assessed by measuring the neuromuscular jitter, which reflects the time variability of processes in the motor end-plate. Jitter is increased in any condition with disturbed end-plate function, such as myasthenic conditions and ongoing reinnervation. Jitter is increasingly being measured with concentric needle (CN) electrodes, which are more prone to artefacts than single fiber EMG recordings.The objective of this review is to identify and demonstrate pitfalls that can be seen with CN jitter measurements, made with both voluntary activation and electrical stimulation.With voluntary activation, errors are caused by poor signal quality; inappropriate time reference points on the signal; an irregular firing rate; and signals with dual latencies, i.e., "flip-flop." With electrical stimulation, additional errors result from insufficient stimulation intensity; from abrupt change in firing rate; and from axon reflexes.Many pitfalls cannot be avoided during recording and can only be detected during post-processing.It is critical to be aware of these artefacts when measuring jitter with CN electrodes.
http://ift.tt/2xqOEtL
Eye movements in genetic parkinsonisms affecting the α-synuclein, PARK9, and manganese network
Source:Clinical Neurophysiology
Author(s): Elena Pretegiani, Francesca Rosini, Antonio Federico, Lance M. Optican, Alessandra Rufa
http://ift.tt/2wEMyGZ
Efficacy of long pulse Nd:YAG laser versus fractional Er:YAG laser in the treatment of hand wrinkles
Abstract
There are different modalities for hand rejuvenation. Fractional Er:YAG laser and long pulse Nd:YAG laser were introduced for treating hand wrinkles. We plan to compare fractional Er:YAG laser and long pulse Nd:YAG laser in a randomized controlled double-blind design with multiple sessions and larger sample size in comparison with previous studies. Thirty-three participants with hand wrinkles entered this study. They were randomly allocated to undergo three monthly laser treatments on each hand, one with a fractional Er:YAG laser and the other with a long pulse Nd:YAG laser. The evaluations included assessment of clinical improvement determined by two independent dermatologists not enrolled in the treatment along with measuring skin biomechanical property of hands using a sensitive biometrologic device with the assessment of cutaneous resonance running time (CRRT). Moreover, potential side effects and patients' satisfaction have been documented at baseline, 1 month after each treatment, and 3 months after the final treatment session. Clinical evaluation revealed both modalities significantly reduce hand wrinkles (p value < 0.05), with no significant difference between two lasers. Mean CRRT values also decreased significantly after the laser treatment compared to those of the baseline in both laser groups. There was no serious persistent side effect after both laser treatments. Both fractional Er:YAG and long pulse Nd:YAG lasers show substantial clinical improvement of hand skin wrinkles with no serious side effects. However, combination treatment by these lasers along with the other modalities such as fat transfer could lead to better outcomes in hand rejuvenation. Trial registration: IRCT2016032020468N4
http://ift.tt/2wEvnVZ
Σάββατο 7 Οκτωβρίου 2017
Bilateral extracephalic transcranial direct current stimulation improves endurance performance in healthy individuals
Publication date: Available online 5 October 2017
Source:Brain Stimulation
Author(s): L. Angius, A.R. Mauger, J. Hopker, A. Pascual-Leone, E. Santarnecchi, S.M. Marcora
BackgroundTranscranial direct current stimulation (tDCS) has been used to enhance endurance performance but its precise mechanisms and effects remain unknown.ObjectiveTo investigate the effect of bilateral tDCS on neuromuscular function and performance during a cycling time to task failure (TTF) test.MethodsTwelve participants in randomized order received a placebo tDCS (SHAM) or real tDCS with two cathodes (CATHODAL) or two anodes (ANODAL) over bilateral motor cortices and the opposite electrode pair over the ipsilateral shoulders. Each session lasted 10 min and current was set at 2 mA. Neuromuscular assessment was performed before and after tDCS and was followed by a cycling time to task failure (TTF) test. Heart rate (HR), ratings of perceived exertion (RPE), leg muscle pain (PAIN) and blood lactate accumulation (ΔB[La−]) in response to the cycling TTF test were measured.ResultsCorticospinal excitability increased in the ANODAL condition (P < 0.001) while none of the other neuromuscular parameters showed any change. Neuromuscular parameters did not change in the SHAM and CATHODAL conditions. TTF was significantly longer in the ANODAL (P = 0.003) compared to CATHODAL and SHAM conditions (12.61 ± 4.65 min; 10.61 ± 4.34 min; 10.21 ± 3.47 min respectively), with significantly lower RPE and higher ΔB[La−] (P < 0.001). No differences between conditions were found for HR (P = 0.803) and PAIN during the cycling TTF test (P = 0.305).ConclusionOur findings demonstrate that tDCS with the anode over both motor cortices using a bilateral extracephalic reference improves endurance performance.
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Correlation of lysosome-associated protein transmembrane-4β gene overexpression with the malignant phenotypes of hepatocellular carcinoma
Publication date: Available online 7 October 2017
Source:Pathology - Research and Practice
Author(s): Hongying Su, Tao Xu, Xiongfei Huang, Shengbing Zang, Bin Wang, Yang Huang, Jingfeng Liu, Aimin Huang
Hepatocellular carcinoma (HCC) is one of the most common and rapidly fatal malignancies, for which hepatitis B virus (HBV) chronic infection constitutes a major risk factor. The lysosome-associated protein transmembrane-4 β gene (LAPTM4B) is a recently identified gene that was found to be overexpressed in several types of cancer. However, the role of LAPTM4B in HCC tumorigenesis and progression has not been clearly determined. The present study demonstrated that the mRNA as well as the protein levels of LAPTM4B were significantly upregulated in HCC specimens. Patients with higher levels of LAPTM4B mRNA in their HCC tissues tended to be of a younger age, HBsAg+, with an advanced Barcelona Clinic Liver Cancer stage. Moreover, LAPTM4B mRNA expression was positively associated with serum α −fetoprotein levels. We also observed that LAPTM4B was able to promote HCC cell proliferation, migration and invasion in vitro. In conclusion, our results indicated that LAPTM4B plays an important role in the promotion of hepatocarcinogenesis and cancer progression and may serve as a biomarker for the diagnosis and monitoring of HCC.
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