Scholar : Decannulation - νέα αποτελέσματα

Early Tracheostomy in Patients with Traumatic Cervical Spinal Cord Injury Appears Safe and May Improve Outcomes

C Flanagan, BR Childs, TA Moore, HA Vallier - Spine, 2017

Study Design. Retrospective case series Objective. To
characterize outcomes associated with tra.

F-134 Duration of sweep gas off trial for weaning from venovenous extracorporeal membrane oxygenation

전경만， 나수진， 정치량， 최희정， 조양현， 성기익… - 대한결핵및호흡기학회 추계 …, 2017

… 초록(외국어). Background: There is no data on how long time of sweep gas off is enough for
assessing the adequacy of lung function to make a decision of decannulation in patients
receiving venovenous extracorporeal membrane oxygenation (ECMO) …

[PDF] Effects of post-intubation laryngeal stenosis treatment depending on the grade of stenosis

L Zawadzka-Głos, K Ślączka - 2017

… Within a decade of our experience based on 92 patients with subglottic stenosis managed with
endoscopic argon plasma coagulation, we have ob- tained the following outcomes: the treatment
result was positive in 82 patients: decannulation was possible in 44 cases whereas …

Percutaneous versus surgical strategy for tracheostomy: a systematic review and meta-analysis of perioperative and postoperative complications

R Klotz, P Probst, M Deininger, U Klaiber, K Grummich… - Langenbeck's Archives of …, 2017

Page 1. SYSTEMATIC REVIEWS AND META-ANALYSES Percutaneous versus
surgical strategy for tracheostomy: a systematic review and meta-analysis of
perioperative and postoperative complications Rosa Klotz1,2 & Pascal …

 

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Scholar : Myringotomy and tubes - νέα αποτελέσματα

Audiometric assessment of pediatric patients with cystic fibrosis

KL Kreicher, MJ Bauschard, CS Clemmens, CM Riva… - Journal of Cystic Fibrosis, 2017

… These included: otologic, ocular, neurological, cardiac, pulmonary, and gastrointestinal disorders.
Specific otologic conditions of interest included: otitis media, Eustachian tube dysfunction, otorrhea,
cerumen impaction, cholesteatoma, and placement of ear tubes …

Хирургическое лечение пациента с посттравматической сирингомиелией, ассоциированной со сложной посттравматической деформацией позвоночника

АА Зуев, ВБ Лебедев, ДС Епифанов… - Хирургия позвоночника, 2017

… A Disorder of CSF Circulation. Springer, 2014. 12. Leschke JM, Mumert ML, Kurpad SN.
Syringosubarachnoid shunting using a myringotomy tube. Surg Neurol Int. 2016;7(Suppl 1):
S8–S11. DOI: 10.4103/2152-7806.173559. 13. Klekamp J, Batzdorf U, Samii M, Bothe HW …

 

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Scholar : Cricotracheal resection - νέα αποτελέσματα

[PDF] Effects of post-intubation laryngeal stenosis treatment depending on the grade of stenosis

L Zawadzka-Głos, K Ślączka - 2017

… 15. Hartley BE, Rutter MJ, Cotton RT: Cricotracheal resection as a primary procedu- re
for laryngotracheal stenosis in children … 16. Hartley BE, Cotton RT: Paediatric airway
stenosis: laryngotracheal reconstruction or cricotracheal resection …

[PDF] IV Mosin

ИВ Мосин - 2017

… and cricotracheal resection and anastomosis for inflammatory and neoplastic stenoses. Ann Otol
Rhinol Laryngol. 2014 Nov; 123(11): 798-804. doi: 10.1177/0003489414538764. 18. Regnard
JF, Fourquier P., Levasseur P. Results and prognostic factors in resections of primary …

 

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Scholar : ΣΤΟΜΑΤΙΚΗ - νέα αποτελέσματα

Λέξεις-κλειδιά: Στοματική υγιεινή, στάσεις, γνώσεις, Διασωληνωμένους ασθενείς, Νοσηλευτές εντατικής θεραπείας, Πρακτικές ΝΟΣΗΛΕΥΤΙΚΗ, 55 (1), 41-51, 2016 …

Ξ Ευαγγέλου, Μ Χατζημπαλάση

Εισαγωγή: Πολλές ερευνητικές μελέτες έχουν δείξει ότι η φροντίδα της στοματικής κοιλότητας
παίζει καθοριστικό ρόλο στη στοματική υγιεινή των ασθενών υπό μηχανική υποστήριξη της αναπνοής
μέσω ενδοτραχειακού σωλήνα, μειώνοντας την επίπτωση νοσοκομειακών λοιμώξεων, συμπεριλαμβανομένης

Λέξεις-κλειδιά: Στοματική υγεία, Iκανοποίηση, Δείκτης DMFT, Οδοντίατρος, ΕΟΠΥΥ ΝΟΣΗΛΕΥΤΙΚΗ, 55 (2), 158-166, 2016-Ερευνητική εργασία

Δ Βάγιας, Γ Αλεξιάς, Π Κωσταγιόλας, Γ Ίντας

Εισαγωγή: Η ποιότητα των υπηρεσιών υγείας και η αναγκαία αξιολόγησή τους, προκειμένου να
εκτιμηθεί κατά πόσο ανταποκρίνονται στους προκαθορισμένους στόχους, αποτελεί ζητούμενο
στις σύγχρονες κοινωνίες. Η ικανοποίηση των ασθενών αποτελεί διάσταση της ποιότητας των

Θ. ΣΤΕΦΑΝΙΩΤΗΣ, Σ. ΓΑΒΑΛΑ, Α. ΔΟΝΤΑ, Κ. ΤΣΙΧΛΑΚΗΣ

Δ ΔΙΑΤΑΡΑΧΕΣ, ΣΤΗ ΓΕΥΣΗ

 

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Scholar : ΑΠΟΚΑΤΑΣΤΑΣΗ - νέα αποτελέσμ

Ανατομική μελέτη των κρημνών των διατιτραινουσών της οπίσθιας κνημιαίας αρτηρίας και κλινικές εφαρμογές

Γ Δριμούρας - 2017

… Περίληψη. Εισαγωγή: Οι κρημνοί των διατιτραινουσών, μισχωτοί ή ελεύθεροι, κατέχουν ουσιαστική
θέση στην αποκατάσταση της κνήμης … Εισαγωγή: Οι κρημνοί των διατιτραινουσών, μισχωτοί ή
ελεύθεροι, κατέχουν ουσιαστική θέση στην αποκατάσταση της κνήμης …

Η επίδραση ενός προγράμματος αποκατάστασης στη μυϊκή λειτουργικότητα του βαρέως πάσχοντος μετά την έξοδο από τη Μονάδα Εντατικής Θεραπείας

Ε Πατσάκη - 2017

… Καθηγήτρια Βασιλειάδης Ιωάννης Επ Καθηγητής. Επιστημονικό πεδίο. Ιατρική &
Επιστήμες Υγείας Επιστήμες Υγείας. Λέξεις-κλειδιά. Αποκατάσταση βαρέως πάσχον.
Χώρα. Ελλάδα. Γλώσσα. Ελληνικά. Άλλα στοιχεία. εικ., πιν., σχημ …

 

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Scholar : ΦΩΝΗ - νέα αποτελέσματα

[PDF] Ραδιόφωνο και εκπαίδευση: απόψεις εκπαιδευτικών για τη συμβολή του European School Radio στην εκπαιδευτική διαδικασία

Α Γενιτζές - 2017

Page 1. ΣΧΟΛΗ ΚΟΙΝΩΝΙΚΩΝ, ΑΝΘΡΩΠΙΣΤΙΚΩΝ ΕΠΙΣΤΗΜΩΝ ΚΑΙ ΤΕΧΝΩΝ ΤΜΗΜΑ
ΕΚΠΑΙΔΕΥΤΙΚΗΣ ΚΑΙ ΚΟΙΝΩΝΙΚΗΣ ΠΟΛΙΤΙΚΗΣ ΠΡΟΓΡΑΜΜΑ ΜΕΤΑΠΤΥΧΙΑΚΩΝ ΣΠΟΥΔΩΝ
ΕΠΙΣΤΗΜΕΣ ΤΗΣ ΕΚΠΑΙΔΕΥΣΗΣ ΚΑΙ ΤΗΣ ΔΙΑ ΒΙΟΥ ΜΑΘΗΣΗΣ …

 

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Cerebral Embolism Following Transcarotid Transcatheter Aortic Valve Replacement

http://ift.tt/2lDOM5c

Silent Myocardial Infarction: Listen to the Evidence

http://ift.tt/2lAQnIS

Silent Myocardial Infarction and Long-Term Risk of Heart Failure: The ARIC Study

AbstractBackground

Although silent myocardial infarction (SMI) accounts for about one-half of the total number of myocardial infarctions (MIs), the risk of heart failure (HF) among patients with SMI is not well established.

Objectives

The purpose of this study was to examine the association of SMI and clinically manifested myocardial infarction (CMI) with HF, as compared with patients with no MI.

Methods

This analysis included 9,243 participants from the ARIC (Atherosclerosis Risk In Communities) study who were free of cardiovascular disease at baseline (ARIC visit 1: 1987 to 1989). SMI was defined as electrocardiographic evidence of MI without CMI after the baseline until ARIC visit 4 (1996 to 1998). HF events were ascertained starting from ARIC visit 4 until 2010 in individuals free of HF before that visit.

Results

Between ARIC visits 1 and 4, 305 SMIs and 331 CMIs occurred. After ARIC visit 4 and during a median follow-up of 13.0 years, 976 HF events occurred. The incidence rate of HF was higher in both CMI and SMI participants than in those without MI (incidence rates per 1,000 person-years were 30.4, 16.2, and 7.8, respectively; p < 0.001). In a model adjusted for demographics and HF risk factors, both SMI (hazard ratio [HR]: 1.35; 95% confidence interval [CI]: 1.02 to 1.78) and CMI (HR: 2.85; 95% CI: 2.31 to 3.51) were associated with increased risk of HF compared with no MI. These associations were consistent in subgroups of participants stratified by several HF risk predictors. However, the risk of HF associated with SMI was stronger in those younger than the median age (53 years) (HR: 1.66; 95% CI: 1.00 to 2.75 vs. HR: 1.19; 95% CI: 0.85 to 1.66, respectively; overall interaction p by MI type <0.001).

Conclusions

SMI is associated with an increased risk of HF. Future research is needed to examine the cost effectiveness of screening for SMI as part of HF risk assessment, and to identify preventive therapies to improve the risk of HF among patients with SMI.

http://ift.tt/2Eunnvg

Early Experience With New Transcatheter Mitral Valve Replacement

AbstractBackground

Transcatheter mitral valve replacement (TMVR) is a potential therapy for patients with symptomatic, severe mitral regurgitation (MR). The feasibility of this therapy remains to be defined.

Objectives

The authors report their early experience with TMVR using a new valve system.

Methods

The valve is a self-expanding, nitinol valve with bovine pericardial leaflets that is placed using a transapical delivery system. Patients with symptomatic MR who were deemed high or extreme risk by the local heart teams were enrolled in a global pilot study at 14 sites (United States, Australia, and Europe).

Results

Fifty consecutively enrolled patients (mean age: 73 ± 9 years; 58.0% men; 84% secondary MR) underwent TMVR with the valve. The mean Society for Thoracic Surgery score was 6.4 ± 5.5%; 86% of patients were New York Heart Association functional class III or IV, and the mean left ventricular ejection fraction was 43 ± 12%. Device implant was successful in 48 patients with a median deployment time of 14 min (interquartile range: 12 to 17 min). The 30-day mortality was 14%, with no disabling strokes, or repeat interventions. Median follow-up was 173 days (interquartile range: 54 to 342 days). At latest follow-up, echocardiography confirmed mild or no residual MR in all patients who received implants. Improvements in symptom class (79% in New York Heart Association functional class I or II at follow-up; p < 0.0001 vs. baseline) and Minnesota Heart Failure Questionnaire scores (56.2 ± 26.8 vs. 31.7 ± 22.1; p = 0.011) were observed.

Conclusions

TMVR with the valve was feasible in a study group at high or extreme risk for conventional mitral valve replacement. These results inform trial design of TMVR in lower-risk patients with severe mitral valve regurgitation (Evaluation of the Safety and Performance of the Twelve Intrepid Transcatheter Mitral Valve Replacement System in High Risk Patients with Severe, Symptomatic Mitral Regurgitation – The Twelve Intrepid TMVR Pilot Study; NCT02322840)

http://ift.tt/2Eqha3b

Reply: An Incomplete Story

http://ift.tt/2lDNCGD

JACC Instructions for Authors

http://ift.tt/2EtJMZy

Sham-Controlled Trials for Coronary Interventions: Ethically Acceptable and Ethically Important

http://ift.tt/2lCjXh7

Transcatheter Mitral Valve Replacement: First Steps on a Long Road

http://ift.tt/2lAp81h

An Incomplete Story

http://ift.tt/2lDiEP7

Beating-Heart Mitral Valve Repair Using a Novel ePTFE Cordal Implantation Device: A Prospective Trial

AbstractBackground

Conventional mitral valve (MV) operations allow direct anatomic assessment and repair on an arrested heart, but require cardiopulmonary bypass, aortic cross-clamping, sternotomy or thoracotomy, and cardioplegic cardiac arrest, and are associated with significant perioperative disability, and risks of morbidity and mortality.

Objectives

This study evaluated safety and performance of a transesophageal echocardiographic-guided device designed to implant artificial expanded polytetrafluoroethylene (ePTFE) cords on mitral leaflets in the beating heart.

Methods

In a prospective multicenter study, 30 consecutive patients with severe degenerative mitral regurgitation (MR) were treated with a mitral valve repair system (MVRS) via small left thoracotomy. The primary (30-day) endpoint was successful implantation of cords with MR reduction to moderate or less.

Results

The primary endpoint was met in 27 of 30 patients (90%). Three patients required conversion to open mitral surgery. There were no deaths, strokes, or permanent pacemaker implantations. At 1 month, MR was mild or less in 89% (24 of 27) and was moderate in 11% (3 of 27). At 6 months, MR was mild or less in 85 % (22 of 26), moderate in 8% (2 of 26), and severe in 8% (2 of 26). Favorable cardiac remodeling at 6 months included decreases in end-diastolic (161 ± 36 ml to 122 ± 30 ml; p < 0.001) and left atrial volumes (106 ± 36 ml to 69 ± 24 ml; p < 0.001). The anterior-posterior mitral annular dimension decreased from 34.7 ± 5.8 mm to 28.2 ± 5.1 mm; p < 0.001 as did the mitral annular area (10.0 ± 2.7 cm² vs. 6.9 ± 2.0 cm²; p < 0.0001).

Conclusions

MVRS ePTFE cordal implantation can reduce the invasiveness and morbidity of conventional MV surgery. The device's safety profile is promising and prospective trials comparing the outcomes of the MVRS to conventional MV repair surgery are warranted. (CE Mark Study for the Harpoon Medical Device [TRACER]; NCT02768870)

http://ift.tt/2EtRUt9

Cost-Effectiveness of Long-Term Ticagrelor in Patients With Prior Myocardial Infarction: Analysis by Subgroups

http://ift.tt/2lAVr05

TMVR With Artificial Cords in the Treatment of Mitral Regurgitation: More Questions Than Answers

http://ift.tt/2EtRSl1

Primary Prevention With Statins in the Elderly

Abstract

The burden of atherosclerotic cardiovascular disease (ASCVD) in high-income countries is mostly borne by the elderly. With increasing life expectancy, clear guidance on sensible use of statin therapy to prevent a first and potentially devastating ASCVD event is critically important to ensure a healthy aging population. Since 2013, 5 major North American and European guidelines on statin use in primary prevention of ASCVD have been released by the American College of Cardiology/American Heart Association, the UK National Institute for Health and Care Excellence, the Canadian Cardiovascular Society, U.S. Preventive Services Task Force, and the European Society of Cardiology/European Atherosclerosis Society. Guidance on using statin therapy in primary ASCVD prevention in the growing elderly population (>65 years of age) differs markedly. The authors discuss the discrepant recommendations, place them into the context of available evidence, and identify circumstances in which uncertainty may hamper the appropriate use of statins in the elderly.

http://ift.tt/2Esm9jZ

Temporal Trends of De Novo Malignancy Development After Heart Transplantation

AbstractBackground

Malignancy is a concern in cardiac transplant recipients, but the temporal trends of de novo malignancy development are unknown.

Objectives

The goal of this study was to describe the temporal trends of the incidence, types, and predictors of de novo malignancy in cardiac transplant recipients.

Methods

The authors analyzed the temporal trends of post-transplant incidence, types, and predictors of malignancy using 17,587 primary adult heart-only transplant recipients from the International Society for Heart and Lung Transplantation registry. The main study outcomes included the incidence of, types of, and time to de novo malignancy.

Results

The risk of any de novo solid malignancy between years 1 and 5 after transplantation was 10.7%. The cumulative incidence by malignancy type was: skin cancer (7.0%), non-skin solid cancer (4.0%), and lymphoproliferative disorders (0.9%). There was no temporal difference in the time to development according to malignancy type. However, the cumulative incidence of de novo solid malignancy increased from 2000 to 2005 vs. 2006 to 2011 (10.0% vs. 12.4%; p < 0.0001). Survival in patients after de novo malignancy was markedly lower than in patients without malignancy (p < 0.0001). Older recipients and patients who underwent transplantation in the recent era had a higher risk of de novo malignancy.

Conclusions

More than 10% of adult heart transplant recipients developed de novo malignancy between years 1 and 5 after transplantation, and this outcome was associated with increased mortality. The incidence of post-transplant de novo solid malignancy increased temporally, with the largest increase in skin cancer. Individualized immunosuppression strategies and enhanced cancer screening should be studied to determine whether they can reduce the adverse outcomes of post-transplantation malignancy.

http://ift.tt/2lCjYlb

New Beginnings, New Transitions: A Bold Vision for the Journals Fellows-in-Training and Early Career Page

http://ift.tt/2EsRcMu

Malignancy Post Heart Transplantation: No Free Lunch

http://ift.tt/2EtRRgX

Effects of Direct Oral Anticoagulants at the Peak Phase, Trough Phase, and After Vascular Injury

http://ift.tt/2Ev5Pz4

Platelet-Derived MRP-14 Induces Monocyte Activation in Patients With Symptomatic Peripheral Artery Disease

AbstractBackground

Peripheral artery disease (PAD), a diffuse manifestation of atherothrombosis, is a major cardiovascular threat. Although platelets are primary mediators of atherothrombosis, their role in the pathogenesis of PAD remains unclear.

Objectives

The authors sought to investigate the role of platelets in a cohort of symptomatic PAD.

Methods

The authors profiled platelet activity, mRNA, and effector roles in patients with symptomatic PAD and in healthy controls. Patients with PAD and carotid artery stenosis were recruited into ongoing studies (NCT02106429 and NCT01897103) investigating platelet activity, platelet RNA, and cardiovascular disease.

Results

Platelet RNA sequence profiling mapped a robust up-regulation of myeloid-related protein (MRP)-14 mRNA, a potent calcium binding protein heterodimer, in PAD. Circulating activated platelets were enriched with MRP-14 protein, which augmented the expression of the adhesion mediator, P-selectin, thereby promoting monocyte–platelet aggregates. Electron microscopy confirmed the firm interaction of platelets with monocytes in vitro and colocalization of macrophages with MRP-14 confirmed their cross talk in atherosclerotic manifestations of PAD in vivo. Platelet-derived MRP-14 was channeled to monocytes, thereby fueling their expression of key PAD lesional hallmarks and increasing their directed locomotion, which were both suppressed in the presence of antibody-mediated blockade. Circulating MRP-14 was heightened in the setting of PAD, significantly correlated with PAD severity, and was associated with incident limb events.

Conclusions

The authors identified a heightened platelet activity profile and unraveled a novel immunomodulatory effector role of platelet-derived MRP-14 in reprograming monocyte activation in symptomatic PAD. (Platelet Activity in Vascular Surgery and Cardiovascular Events [PACE]; NCT02106429; and Platelet Activity in Vascular Surgery for Thrombosis and Bleeding [PIVOTAL]; NCT01897103)

http://ift.tt/2lBP0tC

Transcatheter Versus Surgical Aortic Valve Replacement: Unneglectable Concomitant Coronary Artery Disease

http://ift.tt/2Esm6Vl

MRP-14 Preach the Worse for Platelets and Monocytes Union in Peripheral Artery Disease

http://ift.tt/2Et2j8p

Combining advantages of homogeneous organocatalysis and heterogeneous catalysis with thermosensitive single-chain nanoparticles in a representative tetrahydropyranilation of alcohols

Publication date: 31 January 2018
Source:Polymer, Volume 136
Author(s): Misha Rumyantsev, Sergey Rumyantsev
A significant scientific problem solved in this work is the development of effective polymer catalysts that allow carrying out organic reactions under homogeneous conditions with high rates and to remove catalyst from the system as a heterophase without the use of special treatment, thus combining the advantages of both homogeneous and heterogeneous catalysis. Kinetic experiments show high catalytic activity of the synthesized catalyst in homogeneous regime even at low dosage of the catalyst. Thus, it was demonstrated that tetrahydropyranilation of methanol was completed in nearly quantitative yield of the corresponding ether in 80 min at 60 °C with a dosage of the polymeric catalyst as low as 0.2 mol%. On the other hand anomalous decrease in the values of the effective rate constant was observed when the reaction was run at temperatures below the phase transition temperature. Kinetic data were used along with thermodynamic calculations to discuss the effect of the synthesized polymeric catalyst on the stabilization of the transition state. The high activity of the catalyst is complemented by its high sedimentation ability; thus, it usually takes a few minutes for the polymer to precipitate from the alcohol solution when cooled below the phase-transition temperature after the completion of the reaction. It was also revealed via dynamic light scattering that catalytic polymers adopt a thermodynamically stable conformation of the single-chain nanoparticles with average hydrodynamic radius in the range 2–2.5 nm.

Graphical abstract

http://ift.tt/2CqbcRK

Selective synthesis of highly soluble cyclic olefin copolymers with pendant vinyl groups using 1,5-hexadiene as a comonomer

Publication date: 31 January 2018
Source:Polymer, Volume 136
Author(s): Ryo Tanaka, Akane Sasaki, Takuma Takenaka, Yuushou Nakayama, Takeshi Shiono
Copolymerization of norbornene (NB) and 1,5-hexadiene (HD) was performed using a fluorenylamido-ligated titanium catalyst which conducts copolymerization of NB and 1-alkene. The obtained copolymers possessed pendant vinyl groups, of which content was varied from 0 to 9.4% by the initial feed ratio of the monomers. The copolymers were soluble in various solvents even at high conversion (>85%), showing that the crosslinking of pendant vinyl groups was negligible. The glass transition temperature of the copolymer was changed from 79 °C to over 300 °C, depending on the comonomer composition. Post-functionalization of the pendant vinyl groups with 3,3,4,4,5,5,6,6,6-nonafluoro-1-hexene via ruthenium-catalyzed metathesis reaction was also performed and fluoroalkyl-functionalized cyclic olefin copolymer was successfully obtained. The fluorinated copolymer film showed higher contact angle than the unfunctionalized copolymer.

Graphical abstract

http://ift.tt/2CpEMH1

Effects of pH on the photophysics of conjugated polyelectrolyte complexes

Publication date: 31 January 2018
Source:Polymer, Volume 136
Author(s): Pamela Schleissner, Alexander L. Ayzner
We have previously reported the formation of complexes between oppositely charged, conjugated polyelectrolytes (CPECs), resulting in electronic energy transfer between the donor and acceptor components. The ionic self-assembly process that forms CPECs is heavily influenced by environmental circumstances including solvent composition, temperature, and pH. In this article, we report the effect of polyelectrolyte chain protonation on complex formation and resulting energy transfer. The particular polyelectrolytes used in this study were a pH inactive energy donor and an acceptor polymer with protonatable sidechains. We find that over a large range of acidic pH, the optical properties do not change appreciably up to precipitation (pH 3). Surprisingly in the basic regime where the acceptor polymer is fully deprotonated, complex formation is hindered. However, at pH 11, which corresponds to an excess ion concentration of 0.001 M, the photophysical properties of the complex begin to once again resemble that of the neutral or acidic environments. Our results show that the CPEC displays impressive stability over a relatively broad range of proton concentrations, which may have implications for the construction of supramolecular light-harvesting assemblies.

Graphical abstract

http://ift.tt/2Ctk60M

Near infrared hyperspectral dataset of healthy and infected apple tree leaves images for the early detection of apple scab disease

Publication date: February 2018
Source:Data in Brief, Volume 16
Author(s): Maroua Nouri, Nathalie Gorretta, Pierre Vaysse, Michel Giraud, Christian Germain, Barna Keresztes, Jean-Michel Roger
This dataset presents two series of hyperspectral images of healthy and infected apple tree leaves acquired daily, from two days after inoculation until an advanced stage of infection (11 days after inoculation). The hyperspectral images were calibrated by reflection correction and registered to match the geometry of one reference image. On the last experiment day, scab positions are provided.



http://ift.tt/2CrQyRa

Fibroblast and keratinocyte gene expression following exposure to extracts of neem plant (Azadirachta indica)

Publication date: February 2018
Source:Data in Brief, Volume 16
Author(s): Takao Someya, Katsura Sano, Kotaro Hara, Yoshimasa Sagane, Toshihiro Watanabe, R.G.S. Wijesekara
This data article provides gene expression profiles, determined by using real-time PCR, of fibroblasts and keratinocytes treated with 0.01% and 0.001% extracts of neem plant (Azadirachta indica), local name "Kohomba" in Sri Lanka, harvested in Sri Lanka. For fibroblasts, the dataset includes expression profiles for genes encoding hyaluronan synthase 1 (HAS1), hyaluronan synthase 2 (HAS2), hyaluronidase-1 (HYAL1), hyaluronidase-2 (HYAL2), versican, aggrecan, CD44, collagen, type I, alpha 1 (COL1A1), collagen, type III, alpha 1 (COL3A1), collagen, type VII, alpha 1 (COL7A1), matrix metalloproteinase 1 (MMP1), acid ceramidase, basic fibroblast growth factor (bFGF), fibroblast growth factor-7 (FGF7), vascular endothelial growth factor (VEGF), interleukin-1 alpha (IL-1α), cyclooxygenase-2 (cox2), transforming growth factor beta (TGF-β), and aquaporin 3 (AQP3). For keratinocytes, the expression profiles are for genes encoding HAS1, HAS2, HYAL1, HYAL2, versican, CD44, IL-1α, cox2, TGF-β, AQP3, Laminin5, collagen, type XVII, alpha 1 (COL17A1), integrin alpha-6 (ITGA6), ceramide synthase 3 (CERS3), elongation of very long chain fatty acids protein 1 (ELOVL1), elongation of very long chain fatty acids protein 4 (ELOVL4), filaggrin (FLG), transglutaminase 1 (TGM1), and keratin 1 (KRT1). The expression profiles are provided as bar graphs.



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Data on a single oral dose of camu camu (Myrciaria dubia) pericarp extract on flow-mediated vasodilation and blood pressure in young adult humans

Publication date: February 2018
Source:Data in Brief, Volume 16
Author(s): Tadayoshi Miyashita, Ryosuke Koizumi, Takao Myoda, Yoshimasa Sagane, Koichi Niwa, Toshihiro Watanabe, Kazuhiro Minami
This data article describes the flow-mediated vasodilation (FMD) responses, represented by changes in arterial diameter, and blood pressure changes in young adults after a single oral dose of camu camu (Myrciaria dubia) pericarp extract or placebo (cross-over design). Ten healthy men and 10 healthy women participated in this study. Ultrasonic diagnostic equipment was used to monitor arterial diameter changes, indicative of FMD, for 110s after the administration of the camu camu extract or placebo. In addition, the systolic and diastolic blood pressure values were recorded.



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Data on a delivery of biomolecules into Nicothiana benthamiana leaves using different nanoparticles

Publication date: February 2018
Source:Data in Brief, Volume 16
Author(s): Antonida V. Makhotenko, Ekaterina A. Snigir, Natalia O. Kalinina, Valentin V. Makarov, Michael E. Taliansky
Nanoparticles (NPs) have a number of unique properties associated with their ultrasmall size and exhibit many advantages compared with existing plant biotechnology platforms for delivery of proteins, RNA and DNA of various sizes into the plant cells (Arruda et al., 2015; Silva et al., 2010; Martin-Ortigosa et al., 2014; Mitter et al., 2017) [1–4]. The data presented in this article demonstrate a delivery of biomolecules into Nicotiana benthamiana plant leaves using various types of NPs including gold, iron oxide and chitosan NPs and methods of biolistic bombardment and infiltration. The data demonstrate physical characteristics of NPs coated with fluorescently labeled protein and small RNA (size and zeta-potential) and visualization of nanocomplexes delivery into cells of N. benthamiana leaves by fluorescence microscopy.



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Molecular imaging of tumor photoimmunotherapy: Evidence of photosensitized tumor necrosis and hemodynamic changes

Publication date: 20 February 2018
Source:Free Radical Biology and Medicine, Volume 116
Author(s): Shun Kishimoto, Nobu Oshima, Kazutoshi Yamamoto, Jeeva Munasinghe, Jan Henrik Ardenkjaer-Larsen, James B. Mitchell, Peter L. Choyke, Murali C. Krishna
Near-infrared photoimmunotherapy (NIR PIT) employs the photoabsorbing dye IR700 conjugated to antibodies specific for cell surface epidermal growth factor receptor (EGFR). NIR PIT has shown highly selective cytotoxicity in vitro and in vivo. Cell necrosis is thought to be the main mode of cytotoxicity based mainly on in vitro studies. To better understand the acute effects of NIR PIT, molecular imaging studies were performed to assess its cellular and vascular effects.In addition to in vitro studies for cytotoxicity of NIR PIT, the in vivo tumoricidal effects and hemodynamic changes induced by NIR PIT were evaluated by ¹³C MRI using hyperpolarized [1,4-¹³C2] fumarate, R2* mapping from T2*-weighted MRI, and photoacoustic imaging. In vitro studies confirmed that NIR PIT resulted in rapid cell death via membrane damage, with evidence for rapid cell expansion followed by membrane rupture. Following NIR PIT, metabolic MRI using hyperpolarized fumarate showed the production of malate in EGFR-expressing A431 tumor xenografts, providing direct evidence for photosensitized tumor necrosis induced by NIR PIT. R2* mapping studies showed temporal changes in oxygenation, with an accompanying increase of deoxyhemoglobin at the start of light exposure followed by a sustained decrease after cessation of light exposure. This result suggests a rapid decrease of blood flow in EGFR-expressing A431 tumor xenografts, which is supported by the results of the photoacoustic imaging experiments. Our findings suggest NIR PIT mediates necrosis and hemodynamic changes in tumors by photosensitized oxidation pathways and that these imaging modalities, once translated, may be useful in monitoring clinical treatment response.

Graphical abstract

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