Lipidomic data on lipid droplet triglyceride remodelling associated with protection of breast cancer cells from lipotoxic stress

Publication date: June 2018
Source:Data in Brief, Volume 18
Author(s): Eva Jarc, Thomas O. Eichmann, Robert Zimmermann, Toni Petan
The data presented here is related to the research article entitled "Lipid droplets induced by secreted phospholipase A2 and unsaturated fatty acids protect breast cancer cells from nutrient and lipotoxic stress" by E. Jarc et al., Biochim. Biophys. Acta 1863 (2018) 247–265. Elevated uptake of unsaturated fatty acids and lipid droplet accumulation are characteristic of aggressive cancer cells and have been associated with the cellular stress response. The present study provides lipidomic data on the triacylglycerol (TAG) and phosphatidylcholine (PC) composition of MDA-MB-231 breast cancer cells exposed to docosahexaenoic acid (DHA; 22:6, ω-3). Datasets provide information on the changes in lipid composition induced by depletion of adipose triglyceride lipase (ATGL) and by exogenous addition of secreted phospholipase A2 (sPLA2) in DHA-treated cells. The presented alterations in lipid composition, mediated by targeting lipid droplet biogenesis and lipolysis, are associated with protection from lipotoxicity and allow further investigation into the role of lipid droplets in the resistance of cancer cells to lipotoxic stress.



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A resource for sustainable management: De novo assembly and annotation of the liver transcriptome of the Atlantic chub mackerel, Scomber colias

Publication date: June 2018
Source:Data in Brief, Volume 18
Author(s): André M. Machado, Mónica Felício, Elza Fonseca, Rute R. da Fonseca, L. Filipe C. Castro
Mackerels represent a valuable fishery worldwide. Their ample geographic distribution and capture levels make them an insightful model to address stock management strategies in the context of global changes. Yet, and despite recent impressive genome and transcriptome sequencing efforts from teleost species, available resources from the Scombridae family are comparatively scarce. Here, we generated the first high-quality de novo assembly of the liver transcriptome of the Atlantic chub mackerel (Scomber colias). Through the use of RNA-Seq Illumina technology, 111,124,228 clean reads were obtained for the liver transcriptome. De novo assembly resulted in 93,731 transcripts with an N50 of 1462 bp. This dataset provides an important insight into the context of fisheries management.



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Genome sequence data from 17 accessions of Ensete ventricosum, a staple food crop for millions in Ethiopia

Publication date: June 2018
Source:Data in Brief, Volume 18
Author(s): Zerihun Yemataw, Sadik Muzemil, Daniel Ambachew, Leena Tripathi, Kassahun Tesfaye, Alemayheu Chala, Audrey Farbos, Paul O'Neill, Karen Moore, Murray Grant, David J. Studholme
We present raw sequence reads and genome assemblies derived from 17 accessions of the Ethiopian orphan crop plant enset (Ensete ventricosum (Welw.) Cheesman) using the Illumina HiSeq and MiSeq platforms. Also presented is a catalogue of single-nucleotide polymorphisms inferred from the sequence data at an average density of approximately one per kilobase of genomic DNA.



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Data supporting Ni-NTA magnetic bead-based fluorescent protease assay using recombinant fusion protein substrates

Publication date: June 2018
Source:Data in Brief, Volume 18
Author(s): János András Mótyán, Márió Miczi, Beáta Bozóki, József Tőzsér
Data provided here are related to the research article entitled as 'A recombinant fusion protein-based, fluorescent protease assay for high throughput-compatible substrate screening'. Here we describe data related to the investigation of the properties of the His6-MBP-VSQNY↓PIVQ-mApple recombinant protein substrate and its interactions with Ni-NTA magnetic beads, including the dependence of substrate attachment on incubation time and concentration. Data on the folding efficiency and conformational stability of the recombinant substrate assessed by tryptic digestion are also presented. We describe here the changes of fluorescent properties and binding abilities upon treatments commonly used for stopping enzymatic reactions: trichloroacetic acid (TCA) or heat treatment.



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Data from a targeted proteomics approach to discover biomarkers in saliva for the clinical diagnosis of periodontitis

Publication date: June 2018
Source:Data in Brief, Volume 18
Author(s): V. Orti, B. Mertens, J. Vialaret, P. Gibert, A. Relaño-Ginés, S. Lehmann, D. Deville de Périère, C. Hirtz
This study focused on the search for new biomarkers based on liquid chromatography-multiple reaction monitoring (LC-MRM) proteomics profiling of whole saliva from patients with periodontitis compared to healthy subjects. The LC-MRM profiling approach is a new and innovative method that has already been validated for the absolute and multiplexed quantification of biomarkers in several diseases. The dataset for this study was produced using LC-MRM to monitor protein levels in a multiplex assay, it provides clinical information on salivary biomarkers of periodontitis. The data presented here is an extension of our recently published research article (Mertens et al., 2017) [1].

Graphical abstract

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Survey data on teaching strategies and product innovation: A focus on selected university students in Nigeria

Publication date: June 2018
Source:Data in Brief, Volume 18
Author(s): Maxwell Olokundun, Stephen ibidunni, Mercy Ogbari, Fred Peter, Taiye Borishade, Hezekiah Falola, Odunayo Salau, Oladele Kehinde
The main objective of this study was to examine the effectiveness of the teaching methods adopted in motivating university students' in Nigeria to engage in product innovation. Emphasis was laid on Covenant University in Nigeria which is the pioneer institution to offer entrepreneurship education in Nigeria. The study adopted quantitative method with a descriptive research design to establish trends related to the objective of the study. Survey was be used as quantitative research method. The population of this study comprised all students in the selected university which was given as 6401 3. A sample size of 377 students was selected using yard's formula. Reliability and validity were confirmed. Data was analyzed with the use of Statistical Package for Social Sciences (SPSS).Regression analysis was used as statistical tool of analysis. The field data set is made publicly available to enable critical or a more extensive inquiry.



https://ift.tt/2pz285A

Neuro-fuzzy inference system Prediction of stability indices and Sodium absorption ratio in Lordegan rural drinking water resources in west Iran

Publication date: June 2018
Source:Data in Brief, Volume 18
Author(s): Afshin Takdastan, Majid Mirzabeygi (Radfard), Mahmood Yousefi, Abbas Abbasnia, Rouhollah Khodadadia, Hamed Soleimani, Amir Hossein Mahvi, Davood Jalili Naghan
According to World Health Organization guidelines, corrosion control is an important aspect of safe drinking-water supplies. Water always includes ingredients, dissolved gases and suspended materials. Although some of these water ingredients is indispensable for human beings, these elements more than permissible limits, could be endanger human health. The aim of this study is to assess physical and chemical parameters of drinking water in the rural areas of Lordegan city, also to determine corrosion indices. This cross-sectional study has carried out with 141 taken samples during 2017 with 13 parameters, which has been analyzed based on standard method and to estimate the water quality indices from groundwater using ANFIS. Also with regard to standard conditions, results of this paper are compared with Environmental Protection Agency and Iran national standards. Five indices, Ryznar Stability Index (RSI), Langlier Saturation Index (LSI), Larson-Skold Index (LS), Puckorius Scaling Index (PSI), and Aggressive Index (AI) programmed by using Microsoft Excel software. Owing to its simplicity, the program, can easily be used by researchers and operators. Parameters included Sulfate, Sodium, Chloride, and Electrical Conductivity respectively were 13.5, 28, 10.5, and 15% more than standard level. The amount of Nitrate, in 98% of cases were in permissible limits and about 2% were more than standard level. Result of presented research indicate that water is corrosive at 10.6%,89.4%,87.2%,59.6% and 14.9% of drinking water supply reservoirs, according to LSI, RSI, PSI, LS and AI, respectively.



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Hyperedge bundling: Data, source code, and precautions to modeling-accuracy bias to synchrony estimates

Publication date: June 2018
Source:Data in Brief, Volume 18
Author(s): Sheng H. Wang, Muriel Lobier, Felix Siebenhühner, Tuomas Puoliväli, Satu Palva, J. Matias Palva
It has not been well documented that MEG/EEG functional connectivity graphs estimated with zero-lag-free interaction metrics are severely confounded by a multitude of spurious interactions (SI), i.e., the false-positive "ghosts" of true interactions [1,2]. These SI are caused by the multivariate linear mixing between sources, and thus they pose a severe challenge to the validity of connectivity analysis. Due to the complex nature of signal mixing and the SI problem, there is a need to intuitively demonstrate how the SI are discovered and how they can be attenuated using a novel approach that we termed hyperedge bundling. Here we provide a dataset with software with which the readers can perform simulations in order to better understand the theory and the solution to SI. We include the supplementary material of [1] that is not directly relevant to the hyperedge bundling per se but reflects important properties of the MEG source model and the functional connectivity graphs. For example, the gyri of dorsal-lateral cortices are the most accurately modeled areas; the sulci of inferior temporal, frontal and the insula have the least modeling accuracy. Importantly, we found the interaction estimates are heavily biased by the modeling accuracy between regions, which means the estimates cannot be straightforwardly interpreted as the coupling between brain regions. This raise a red flag that the conventional method of thresholding graphs by estimate values is rather suboptimal: because the measured topology of the graph reflects the geometric property of source-model instead of the cortical interactions under investigation.



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Peptide data on the disulfide bond analysis of baculovirus produced Pfs25 by LC-MSMS

Publication date: June 2018
Source:Data in Brief, Volume 18
Author(s): Shwu-Maan Lee, Jordan L. Plieskatt, C. Richter King
This article contains the peptide data obtained while performing disulfide bond mapping of the recombinant Plasmodium falciparum protein, Pfs25, produced from the baculovirus expression system. Pfs25 is a malaria transmission-blocking vaccine candidate, with a compact and complex structure including 22 cysteines. This supplementary data is related to the research "Disulfide bond mapping of Pfs25, a recombinant malaria transmission blocking vaccine candidate" (Lee et al., 2018) [1]. In brief, Pfs25 was digested with trypsin/Lys-C and derived peptides separated by High Performance Liquid Chromatography (HPLC) and analyzed by mass spectrometry (MS) by MS^E fragmentation. The theoretical peptides and their respective masses along with disulfide bond locations with linked peptides are presented here alongside the mass spectrometry analysis. The raw mass spectrometry data is made available through the Mass Spectrometry Interactive Virtual Environment (MassIVE) with identifier: MSV000081982.



https://ift.tt/2pCzBLt

The influence of KaiA mutations on its function in the KaiABC circadian clock system

Publication date: June 2018
Source:Data in Brief, Volume 18
Author(s): Qiang Chen, Sen Liu, Liting Yang, Lingya Zhang, Jinkui Li
The core oscillator of the circadian clock of cyanobacteria consists of three proteins, KaiA, KaiB, and KaiC. The KaiABC oscillator can be re-constituted in vitro with the purified proteins in buffer containing ATP and Mg²⁺. The interaction between KaiA and KaiC has not been well studied. In this article, different KaiA mutants were designed and used to elucidate the influence of KaiA structure on its function in the in vitro system. Molecular dynamics simulations were adopted to study the structural flexibility of KaiA homodimer. The data presented in this article provide further experimental supports to our work in Chen et al. (2017) [1].



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Evolution over time in the cost-effectiveness of pneumococcal conjugate vaccine (PCV13) in older Australians due to herd protection from infant vaccination

Publication date: 12 April 2018
Source:Vaccine, Volume 36, Issue 16
Author(s): C. Chen, P. Beutels, A.T. Newall
In many settings, serotype changes as a result of infant 13-valent pneumococcal conjugate vaccine (PCV13) programs are likely to continue after the introduction of adult PCV13 programs. We applied a multi-cohort model to explore how potential serotype changes may impact on the cost-effectiveness of PCV13 use in Australian adults aged over 65 years. We found assumptions around continued herd protection from infant PCV13 programs to be critical when assessing the cost-effectiveness of adult PCV13 vaccination in Australia. Future cost-effectiveness analyses of adult PCV13 programs need to carefully consider how to predict these future changes in serotypes, with Australian data suggesting that the changes post-PCV13 use in infants may be different than post-PCV7.



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Oral Chlamydia vaccination induces transmucosal protection in the airway

Publication date: 12 April 2018
Source:Vaccine, Volume 36, Issue 16
Author(s): Cuiming Zhu, Hui Lin, Lingli Tang, Jianlin Chen, Yimou Wu, Guangming Zhong
Although Chlamydia has been frequently detected in the gastrointestinal tracts of both humans and animals, it is not associated with any gastrointestinal pathology. We have recently shown that gastrointestinal Chlamydiamuridarum is not only non-pathogenic but also induces protective immunity in the genital tract. We now report that the transmucosal immunity induced by a single oral immunization with C.muridarum protected the mouse airway from a subsequent challenge infection. The oral immunization significantly reduced chlamydial burden in the airway as early as day 3 after intranasal challenge. As a result, the airway chlamydial spreading to extra-airway tissues was completely prevented on day 3 and significantly reduced on day 9. The immunized mice were protected from any significant systemic toxicity caused by the intranasal challenge since there was no significant bodyweight drop in the immunized mice. This robust protection correlated well with Chlamydia-specific antibodies that recognize chlamydial organism surface antigens and T cell responses that are dominated with a Th1 phenotype. The immunized mice developed high ratios of IgG2b/c over IgG1 levels and IFNγ-producing over IL-5- or IL-13-producing lymphocytes. Thus, we have demonstrated that oral vaccination with C. muridarum can induce Th1-dominant transmucosal immunity in the airway. Together with previous studies, we propose that non-pathogenic colonization of Chlamydia in the gastrointestinal tract be explored as an oral delivery system for inducing protection against infections and pathologies in extra-gastrointestinal tissues.



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Epidemiological profile and progress toward rubella elimination in China. 10 years after nationwide introduction of rubella vaccine

Publication date: 12 April 2018
Source:Vaccine, Volume 36, Issue 16
Author(s): Qiru Su, Chao Ma, Ning Wen, Chunxiang Fan, Hong Yang, Huaqing Wang, Zundong Yin, Zijian Feng, Lixin Hao, Weizhong Yang
BackgroundRubella-containing vaccine (RCV) became available in China in 1993 and was introduced nationwide into the Expanded Immunization Program (EPI) in 2008. We evaluated implementation and impact of RCV from 2 years prior to nationwide introduction through the 10 years after nationwide introduction.MethodsWe analyzed RCV lot-release (doses distributed) data, 1- and 2-dose RCV coverage, and rubella data from China's nationwide disease surveillance system to describe the current status and changes in rubella epidemiology between 2005 and 2017.ResultsWhile the vaccine was included into the routine immunization program in 2008, its full implementation required 4 years due to sporadic vaccine supply constraints. RCV1 and RCV2 coverage increased from 51.5% and 39.0% in 2008 to >95% during 2012 through 2016. From 2005 to 2017, the annual incidences (per million) of rubella ranged from 91.09 in 2008 down to 1.16 in 2017; reductions occurred in all age groups. The proportion of cases among individuals ≥20 years old increased from 0.97% in 2005 to 31.2% in 2017. In the better-developed eastern China, most cases were among adults; in central and western China, most cases were among children or adolescents.ConclusionsThe marked decrease rubella was a result of inclusion of RCVs into EPI targeting children less than 2 years of age and achieving high level of 2-dose coverage. Rubella was reduced in absolute terms, and its epidemiology was changed to older cases with substantial inter-province variation. Ensuring full vaccination of school children and identifying strategies to reach adults with measles and rubella combined vaccines will be important to hasten elimination of rubella and prevent CRS outbreaks.



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Expression and characterization of a novel truncated rotavirus VP4 for the development of a recombinant rotavirus vaccine

Publication date: 12 April 2018
Source:Vaccine, Volume 36, Issue 16
Author(s): Yijian Li, Miaoge Xue, Linqi Yu, Guoxing Luo, Han Yang, Lianzhi Jia, Yuanjun Zeng, Tingdong Li, Shengxiang Ge, Ningshao Xia
The outer capsid protein VP4 is an important target for the development of a recombinant rotavirus vaccine because it mediates the attachment and penetration of rotavirus. Due to the poor solubility of full-length VP4, VP8 was explored as candidate rotavirus vaccines in the past years. In previous studies, it has been found that the N-terminal truncated VP8 protein, VP8-1 (aa26-231), could be expressed in soluble form with improved immunogenicity compared to the core of VP8 (aa65-223). However, this protein stimulated only a weak immune response when aluminum hydroxide was used as an adjuvant. In addition, it should be noted that the protective efficacy of VP4 was higher than that of VP8 and VP5. In this study, it was found that when the N-terminal 25 amino acids were deleted, the truncated VP4^∗ (aa26-476) containing VP8 and the stalk domain of VP5 could be expressed in soluble form in E. coli and purified to homogeneous trimers. Furthermore, the truncated VP4 could induce high titers of neutralizing antibodies when aluminum adjuvant was used and conferred high protective efficacy in reducing the severity of diarrhea and rotavirus shedding in stools in animal models. The immunogenicity of the truncated VP4 was significantly higher than that of VP8^∗ and VP5^∗ alone. Taken together, the truncated VP4^∗ (aa26-476), with enhanced immunogenicity and immunoprotectivity, could be considered as a viable candidate for further development and has the potential to become a parenterally administered rotavirus vaccine.



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Editorial Board/Aims and Scope

Publication date: 12 April 2018
Source:Vaccine, Volume 36, Issue 16





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Enhancing viral vaccine production using engineered knockout vero cell lines – A second look

Publication date: 12 April 2018
Source:Vaccine, Volume 36, Issue 16
Author(s): F. Hoeksema, J. Karpilow, A. Luitjens, F. Lagerwerf, M. Havenga, M. Groothuizen, G. Gillissen, A.A.C. Lemckert, B. Jiang, R.A. Tripp, C. Yallop
The global adoption of vaccines to combat disease is hampered by the high cost of vaccine manufacturing. The work described herein follows two previous publications (van der Sanden et al., 2016; Wu et al., 2017) that report a strategy to enhance poliovirus and rotavirus vaccine production through genetic modification of the Vero cell lines used in large-scale vaccine manufacturing. CRISPR/Cas9 gene editing tools were used to knockout Vero target genes previously shown to play a role in polio- and rotavirus production. Subsequently, small-scale models of current industry manufacturing systems were developed and adopted to assess the increases in polio- and rotavirus output by multiple stable knockout cell lines. Unlike previous studies, the Vero knockout cell lines failed to achieve desired target yield increases. These findings suggest that additional research will be required before implementing the genetically engineered Vero cell lines in the manufacturing process for polio- and rotavirus vaccines to be able to supply vaccines at reduced prices.



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Characterization of capsid protein (p495) of hepatitis E virus expressed in Escherichia coli and assembling into particles in vitro

Publication date: 12 April 2018
Source:Vaccine, Volume 36, Issue 16
Author(s): Minghua Zheng, Jie Jiang, Xiao Zhang, Nan Wang, Kaihang Wang, Qiong Li, Tingting Li, Qingshan Lin, Yingbin Wang, Hai Yu, Ying Gu, Jun Zhang, Shaowei Li, Ningshao Xia
Hepatitis E virus (HEV) is associated with acute hepatitis disease. Numerous truncated HEV capsid proteins have been successfully expressed using different expression systems. Among these, p495, a protein truncated at its N- and C-termini by 111 and 54 amino acids (aa), respectively (HEV ORF2 aa 112–606) can self-assemble into T = 1 virus-like particles (VLPs) when expressed by insect cells. A shorter p239 (aa 368–606) protein is a particulate antigen that we have previously used in our commercialized HEV vaccine, Hecolin. Here, we sought to express p495 in its soluble form (named Ep495) in E. coli and in baculovirus-infected Tn5 insect cells (named BTp495) as a back-to-back control. Characterization of p495 particles derived from these two expression systems showed similarities in particle size, morphology, and sedimentation coefficient. Antigenicity assays using a panel of anti-HEV monoclonal antibodies also showed similar strong reactivities for Ep495 and BTp495, as well as similar binding profiles that were congruent with p239. Mouse immunization results showed that Ep495 particles had comparable immunogenicity with that of BTp495 VLPs, as well as p239. Overall, our findings suggest that p495 particles produced in E. coli are ideal for the development of next-generation prophylactic vaccines against hepatitis E.



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Influenza vaccination among Saudi Hajj pilgrims: Revealing the uptake and vaccination barriers

Publication date: 12 April 2018
Source:Vaccine, Volume 36, Issue 16
Author(s): Mohammad Alfelali, Osamah Barasheed, Al-Mamoon Badahdah, Hamid Bokhary, Mohammed I. Azeem, Turki Habeebullah, Marwan Bakarman, Atif Asghar, Robert Booy, Harunor Rashid
BackgroundHajj is the world's largest annual mass gathering that attracts two to three million Muslims from around the globe to a religious assemblage in Makkah, Saudi Arabia. The risk of acquisition and transmission of influenza among Hajj pilgrims is high. Therefore, influenza vaccination is recommended, and was monitored frequently among pilgrims from different countries. However, the vaccination uptake among Saudi pilgrims has not been assessed in recent years.ObjectiveThis analysis aims to evaluate influenza vaccine uptake among Saudi Hajj pilgrims, and identify the key barriers to vaccination.MethodData on influenza vaccination were obtained from Saudi pilgrims who took part in a large trial during the Hajj of 2013, 2014 and 2015. Pilgrims were met and recruited in Mina, Makkah during the peak period of Hajj and were asked to complete a baseline questionnaire that recorded their influenza vaccination history, including reason(s) for non-receipt of vaccine.ResultsA total of 6974 Saudi pilgrims aged between 18 and 95 (median 34) years were recruited; male to female ratio was 1:1.2. Of the total, 90.8% declared their influenza vaccination history, 51.3% of them reported receiving influenza vaccine before travel to Hajj. The vaccination rates for the years 2013, 2014 and 2015 were 21.4%, 48.2% and 58.1%, respectively (P < 0.001). Of 1,269 pilgrims who were at higher risk of severe disease, 54.5% received the vaccine. Lack of awareness (47.5%), reliance on natural immunity (15.8%) and being busy (15.5%) were the main reasons for non-receipt.ConclusionThese data from a convenience sample indicate that influenza vaccine uptake among Saudi Hajj pilgrims is increasing over years but still needs further improvement. Lack of awareness and misperceptions are the main barriers. Education of Saudi pilgrims and health professionals is required to raise awareness about influenza vaccination. Further studies are needed to understand pilgrims' misperceptions.



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A DNA prime/protein boost vaccine protocol developed against Campylobacter jejuni for poultry

Publication date: 12 April 2018
Source:Vaccine, Volume 36, Issue 16
Author(s): Marine Meunier, Muriel Guyard-Nicodème, Estelle Vigouroux, Typhaine Poezevara, Véronique Béven, Ségolène Quesne, Michel Amelot, Alberto Parra, Marianne Chemaly, Daniel Dory
Vaccination of broilers is one of the potential ways to decrease Campylobacter intestinal loads and therefore may reduce human disease incidence. Despite many studies, no efficient vaccine is available yet. Using the reverse vaccinology strategy, we recently identified new vaccine candidates whose immune and protective capacities need to be evaluated in vivo. Therefore, the goal of the present study was to develop and evaluate an avian subunit vaccine protocol for poultry against Campylobacter jejuni. For this, flagellin was used as vaccine antigen candidate. A DNA prime/protein boost regimen was effective in inducing a massive protective immune response against C. jejuni in specific pathogen free Leghorn chickens. Contrastingly, the same vaccine regimen stimulated the production of antibodies against Campylobacter in conventional Ross broiler chickens harbouring maternally derived antibodies against Campylobacter, but not the control of C. jejuni colonization. These results highlight the strength of the vaccine protocol in inducing protective immunity and the significance of the avian strain and/or immune status in the induction of this response. Nevertheless, as such the vaccine protocol is not efficient in broilers to induce protection and has to be adapted; this has been done in one of our recent published work.



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The impact of [ 68 Ga]PSMA I&T PET/CT on radiotherapy planning in patients with prostate cancer

Abstract

Purpose

To determine the impact of Gallium-68-labled prostate-specific membrane antigen positron-emission tomography/computed tomography ([⁶⁸Ga]PSMA PET/CT) on radiotherapy planning for primary disease, biochemical cancer relapse, and advanced disease of prostate cancer.

Methods

A total of 106 patients with prostate cancer scheduled for radiation therapy underwent 120 [⁶⁸Ga]PSMA PET/CT scans prior to radiotherapy treatment. In 20 cases, patients underwent [⁶⁸Ga]PSMA PET/CT for primary therapy (PT), 75 cases were referred for biochemical relapse after surgery (RL), and 25 cases were intended for palliative treatment of localized metastases (MD). We retrospectively compared the impact of [⁶⁸Ga]PSMA PET/CT on lesion detection and treatment decision to CT alone.

Results

[⁶⁸Ga]PSMA PET/CT revealed a total of 271 positive lesions, whereas CT detected 86 lesions (32%). Overall, the radiotherapy regime was changed in 55 of 120 cases (46%) based on the higher detection rate of [⁶⁸Ga]PSMA PET/CT: in 15% of cases with PT, in 43% of cases with RL, and in 44% of cases with MD.

Conclusion

[⁶⁸Ga]PSMA PET/CT is superior to CT alone for lesion detection in prostate cancer, thereby significantly impacting on radiotherapy planning for primary disease, biochemical cancer relapse, and advanced disease of prostate cancer.

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