RNA Selection by PIWI Proteins

Publication date: Available online 24 January 2018
Source:Trends in Biochemical Sciences
Author(s): Alexey L. Arkov
Gene regulation by PIWI–piRNA complexes is determined by the selection of cognate target RNAs by PIWI–piRNA. What are the mechanisms for this selection? There is a rigorous multistep control in identifying target RNAs by PIWI–piRNA structures, and RNA helicases play a potentially crucial role in this process.



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A prospective 52-week randomised controlled trial of patient-initiated care consultations for patients with psoriasis

Abstract

Background

Treatment and care of moderate to severe psoriasis requires lifelong consultations with a dermatologist with close monitoring of systemic treatment.

Objectives

To investigate the effect of patient-initiated care consultations (PICC) for patients with psoriasis in a dermatology outpatient clinic.

Methods

A prospective randomised controlled trial with patients in well-controlled systemic treatment randomised to either 1) the PICC group, where they participated in one annual consultation with a dermatologist but were able to initiate consultations when needed; or 2) routine care, where they participated in a consultation every 12-16 weeks. The primary outcome was the Dermatology Life Quality Index (DLQI). Other outcomes were safety, patient adherence and satisfaction with healthcare assessed at baseline and after 52 weeks.

Results

150 patients were included, with 58.0% treated with biologicals, 37.3% with methotrexate and 4.7% with acitretin. At week 52 no statistically significant mean difference between groups was detected in DLQI 0.28 (95% CI, -0.35–0.9) or Psoriasis Area Severity Index -0.24 (95% CI, -0.84–0.36). Patients in the PICC group requested 63.1% fewer consultations with a dermatologist, mean ±SD 2.5 ±0.1 vs. 5.1 ±0.6, (p=0.001). Patient adherence and safety with treatment monitoring was equal between groups, but the PICC group was significantly better at attending consultations than the control group (p=0.003).

Conclusion

PICC offers additional clinical benefits compared to routine care, making patients less dependent on clinical visits. The intervention adds no harm to monitoring systemic treatment and patients report high quality of life and satisfaction with healthcare.

This article is protected by copyright. All rights reserved.

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Does goal-directed haemodynamic and fluid therapy improve peri-operative outcomes?: A systematic review and meta-analysis

BACKGROUND Much uncertainty exists as to whether peri-operative goal-directed therapy is of benefit. OBJECTIVES To discover if peri-operative goal-directed therapy decreases mortality and morbidity in adult surgical patients. DESIGN An updated systematic review and random effects meta-analysis of randomised controlled trials. DATA SOURCES Medline, Embase and the Cochrane Library were searched up to 31 December 2016. ELIGIBILITY CRITERIA Randomised controlled trials enrolling adult surgical patients allocated to receive goal-directed therapy or standard care were eligible for inclusion. Trauma patients and parturients were excluded. Goal-directed therapy was defined as fluid and/or vasopressor therapy titrated to haemodynamic goals [e.g. cardiac output (CO)]. Outcomes included mortality, morbidity and hospital length of stay. Risk of bias was assessed using Cochrane methodology. RESULTS Ninety-five randomised trials (11 659 patients) were included. Only four studies were at low risk of bias. Modern goal-directed therapy reduced mortality compared with standard care [odds ratio (OR) 0.66; 95% confidence interval (CI) 0.50 to 0.87; number needed to treat = 59; N = 52; I2 = 0.0%]. In subgroup analysis, there was no mortality benefit for fluid-only goal-directed therapy, cardiac surgery patients or nonelective surgery. Contemporary goal-directed therapy also reduced pneumonia (OR 0.69; 95% CI, 0.51 to 0. 92; number needed to treat = 38), acute kidney injury (OR 0. 73; 95% CI, 0.58 to 0.92; number needed to treat = 29), wound infection (OR 0.48; 95% CI, 0.37 to 0.63; number needed to treat = 19) and hospital length of stay (days) (−0.90; 95% CI, −1.32 to −0.48; I2 = 81. 2%). No important differences in outcomes were found for the pulmonary artery catheter studies, after accounting for advances in the standard of care. CONCLUSION Peri-operative modern goal-directed therapy reduces morbidity and mortality. Importantly, the quality of evidence was low to very low (e.g. Grading of Recommendations, Assessment, Development and Evaluation scoring), and there was much clinical heterogeneity among the goal-directed therapy devices and protocols. Additional well designed and adequately powered trials on peri-operative goal-directed therapy are necessary. Correspondence to Dr Matthew A. Chong, MD, Department of Anesthesia and Perioperative Medicine, University Hospital – London Health Sciences Centre, 339 Windermere Road, C3-108, London, ON, Canada N6A 5A5 E-mail: matthew.a.chong@gmail.com Supplemental digital content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal's Website (http://ift.tt/2ylyqmW). © 2018 European Society of Anaesthesiology

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Comparative efficacy of two anti-aging products containing retinyl palmitate in healthy human volunteers

Summary

Background

No study yet described the comparative efficacy of two over-the-counter (OTC) anti-aging products in Asian subjects using the techniques involving analysis of living skin.

Aim

We sought to evaluate the anti-aging efficacy of two commercial formulations containing retinyl palmitate using a high-resolution UVA video camera.

Method

Total 11 healthy male volunteers, agreed to participate in this single-blind split-face design study with the mean age of 25.5 years. Every night, volunteers applied one type of cream on the left side and other type of cream on the right side of the face, as directed according to the study design for 60 days. Measurements of the parameters were taken at 0, 1st, 7th, 15th, 30th, and 60th day of study period using noninvasive UVA video camera Visioscan^® VC98. Cream applied on right side of the face labeled as "R" and on the left side as "L."

Results

Sixty-day use of the creams showed significant improvement in SELS parameters of the skin. Percent change in skin wrinkling (SEw) parameter calculated after 60 days was −6.68% after applying cream R and −8.27% after applying cream L.

Conclusion

We concluded that constituents in both creams have potentially influenced skin surface parameters, thus indicating that, these creams as a better option to lessen the effects of aging on facial skin on long-term application.

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The use of natural ingredients in innovative Korean cosmeceuticals

Summary

Background

The cosmeceutical industry is an ever-growing and in demand market, especially in Asia. Korea has been on the forefront of creating the newest generation and most innovative cosmeceuticals products including ingredients such as snail secretions, starfish powder, botanical extracts, green tea, and red ginseng. Given their increasing prevalence in the cosmeceutical industry, scientists have been conducting investigations into these extracts and their properties.

Objective

To summarize the current literature surrounding multiple natural ingredients found in Korean cosmeceutical products.

Methods

A review of the literature surrounding natural ingredients found in Korean cosmeceuticals was conducted using PubMed (U.S. National Library of Medicine).

Results

Multiple natural extracts have been found to have antiaging, antitumor, and antimelanogenic effects making them useful additives in current cosmeceutical products.

Conclusion

With the public's increasing awareness of cosmeceutical products, it is important for physicians to understand the properties of these extracts in order to inform patients correctly and ensure patient safety.

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Seborrheic keratoses mimicking melanoma unveiled by in vivo reflectance confocal microscopy

Abstract

Background

Seborrheic keratoses (SebK) with atypical dermoscopy presentation are increasingly reported. These lesions do not exhibit typical dermoscopy features of SebK and sometimes mimic melanoma, thus complicating the differential diagnosis. Reflectance confocal microscopy (RCM) is a non-invasive tool, which allows an in vivo imaging of the skin. The study objectives were to evaluate the agreement between RCM classification and histological diagnoses, and the reliability of well-known RCM criteria for SebK in the identification of SebK with atypical dermoscopy presentation.

Materials and Methods

We retrospectively analysed at RCM excised lesions presenting in dermoscopy ≥1 score at revisited 7-point checklist. The study population consisted of cases showing no melanocytic RCM findings. Lesions were investigated for distinct non-melanocytic RCM features, blinded from histopathology diagnoses. Histopathology matching was then performed before statistical analysis.

Results

The study consisted of 117 cases, classified at RCM as SebK (71 cases), dermatofibroma (18 cases), basal cell carcinoma (13 cases), squamous cell carcinoma (2 cases), and "non-specific" (13 cases). Overall K strength of agreement at histopathology matching proved 0.76. Of the 71 cases classified at RCM with SebK, agreement was achieved in 97%.

Conclusion

Reflectance confocal microscopy classification proved high agreement with histopathology for SebK with atypical dermoscopy presentations, allowing an early differential diagnosis. RCM features in this group of lesions were similar to those described for typical cases of SebK, and may assist clinician therapy decision making, whilst avoiding unnecessary excisions.

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High-frequency ultrasound-based differentiation between nodular dermal filler deposits and foreign body granulomas

Abstract

Background

The number of procedures involving dermal filler injection is still on the rise. Although their safety is improving, not all complications can be avoided. The late complications manifesting as nodules or granulomas pose a particular diagnostic and therapeutic challenge, due to the lack of uniform standards or guidelines. High-frequency, ultrasound imaging appears to be a useful method for distinguishing between granulomas and nodular dermal filler deposits.

Aim

The aim of the paper was to evaluate the utility of high frequency ultrasound imaging for distinguishing between foreign body granulomas and nodular dermal filler deposits.

Material and methods

Eleven females aged 21-66 years (mean age of 43.6 years old) who had soft tissue fillers injected were enrolled. All patients had a high-frequency ultrasound scan of the involved skin area performed. The shape, margins, area, location and echogenicity of the lesions were assessed. Additionally, the lesions were evaluated histologically and photographs were taken.

Results

The analysis indicated differences between the ultrasound image of granulomas and dermal filler deposits. Characteristic ultrasound features of granulomas include oval shape and blurred, irregular outer edges. Small hyperechoic areas were seen inside the granulomas. The deposits were anechogenic, with sharp, regular borders.

Conclusions

High-frequency ultrasound imaging enables distinguishing between granulomas and nodules—dermal filler deposits, which form after dermal filler injections.

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Automated digital image analysis (TrichoScan) in male patients with androgenetic alopecia; comparison with manual marking of hairs on trichoscopic images

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Discrepancy of width between actual cutaneous lesion and punch biopsy specimen

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Physiological and Anatomical Outputs of Rat Genital Cortex

Abstract

Rat somatosensory genital cortex contains a large sexually monomorphic representation of the penis in males and the clitoris in females. Genital cortex microstimulation-evoked movements of legs, trunk and genitals, which showed sex-specific differences related to mating behaviors and included thrusting in males and lordosis-like movements in females. Erections/tumescence of penis or clitoris could not be evoked, however. Anterograde tracer injections into penis/clitoris cortex revealed eleven corticocortical and 10 subcortical projection targets, which were qualitatively similar in both sexes. Corticocortical genital-cortex-projections innervated about 3% of the cortical surface and most were analog to other somatosensory projections targeting motor cortex, secondary somatosensory cortex, parietal cortex and perirhinal cortex. Corticocortical projections that differed from other parts of somatosensory cortex targeted male scrotum cortex, female vulva cortex, the somatosensory–ear–auditory-cortex-region and the caudal parietal area. Aligning cytoarchitectonic borders with motor topography, sensory genital responses and corticocortical projections identified a candidate region for genital motor cortex. Most subcortical genital-cortex-projections were analog to other thalamic, tectal or pontine projections of somatosensory cortex. Genital-cortex-specific subcortical projections targeted amygdala and nucleus submedius and accumbens. Microstimulation-effects and projections support a sexual function of genital cortex and suggest that genital cortex is a major hub of sexual sensorimotor processing in rodents.

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A Brief History of the Encoding of Hand Position by the Cerebral Cortex: Implications for Motor Control and Cognition

Abstract

Encoding hand position by the cerebral cortex is essential not only for the neural representation of the body image but also for different actions based on eye–hand coordination. These include reaching for visual objects as well as complex movement sequences, such as tea-making, tool use, and object construction, among many others. All these functions depend on a continuous refreshing of the hand position representation, relying on both predictive signaling and afferent information. The hand position influence on neural activity in the parietofrontal system, together with eye position signals, are the basic elements of an eye–hand matrix from which all the above functions can emerge and could be regarded as key features of a network with several entry points, command nodes and outflow pathways, as confirmed by the discovery of a direct parietospinal projection for the control of hand action. The integrity of this system is crucial for daily life, as testified by the consequences of cortical lesions, spanning from severe paralysis to complex forms of apraxia. In this review, I will sketch my personal understanding of the scientific and conceptual trajectory of a line of investigation with many unexpected influences on cortical function and disease, from motor behavior to cognition.

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Presynaptic GABAA Receptors Modulate Thalamocortical Inputs in Layer 4 of Rat V1

Abstract

Fast inhibitory GABAergic transmission plays a fundamental role in neural circuits. Current theories of cortical function assume that fast GABAergic inhibition acts via GABA_A receptors on postsynaptic neurons, while presynaptic effects of GABA depend on GABA_B receptor activation. Manipulations of GABA_A receptor activity in vivo produced different effects on cortical function, which were generally ascribed to the mode of action of a drug, more than its site of action. Here we show that in rodent primary visual cortex, α4-containing GABA_A receptors can be located on subsets of glutamatergic and GABAergic presynaptic terminals and decrease synaptic transmission. Our data provide a novel mechanistic insight into the effects of changes in cortical inhibition; the ability to modulate inputs onto cortical circuits locally, via presynaptic regulation of release by GABA_A receptors.

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On the Neural and Mechanistic Bases of Self-Control

Abstract

Intertemporal choice requires a dynamic interaction between valuation and deliberation processes. While evidence identifying candidate brain areas for each of these processes is well established, the precise mechanistic role carried out by each brain region is still debated. In this article, we present a computational model that clarifies the unique contribution of frontoparietal cortex regions to intertemporal decision making. The model we develop samples reward and delay information stochastically on a moment-by-moment basis. As preference for the choice alternatives evolves, dynamic inhibitory processes are executed by way of asymmetric lateral inhibition. We find that it is these lateral inhibition processes that best explain the contribution of frontoparietal regions to intertemporal decision making exhibited in our data.

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Fear Extinction Recall Modulates Human Frontomedial Theta and Amygdala Activity

Abstract

Human functional magnetic resonance imaging (fMRI) and electroencephalography (EEG) studies, as well as animal studies, indicate that the amygdala and frontomedial brain regions are critically involved in conditioned fear and that frontomedial oscillations in the theta range (4–8 Hz) may support communication between these brain regions. However, few studies have used a multimodal approach to probe interactions among these key regions in humans. Here, our goal was to bridge the gap between prior human fMRI, EEG, and animal findings. Using simultaneous EEG–fMRI recordings 24 h after fear conditioning and extinction, conditioned stimuli presented (CS+E, CS−E) and not presented during extinction (CS+N, CS−N) were compared to identify effects specific to extinction versus fear recall. Differential (CS+ vs. CS−) electrodermal, frontomedial theta (EEG) and amygdala responses (fMRI) were reduced for extinguished versus nonextinguished stimuli. Importantly, effects on theta power covaried with effects on amygdala activation. Fear and extinction recall as indicated by theta explained 60% of the variance for the analogous effect in the right amygdala. Our findings show for the first time the interplay of amygdala and frontomedial theta activity during fear and extinction recall in humans and provide insight into neural circuits consistently linked with top-down amygdala modulation in rodents.

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Cortical Responses to Input From Distant Areas are Modulated by Local Spontaneous Alpha/Beta Oscillations

Abstract

Any given area in human cortex may receive input from multiple, functionally heterogeneous areas, potentially representing different processing threads. Alpha (8–13 Hz) and beta oscillations (13–20 Hz) have been hypothesized by other investigators to gate local cortical processing, but their influence on cortical responses to input from other cortical areas is unknown. To study this, we measured the effect of local oscillatory power and phase on cortical responses elicited by single-pulse electrical stimulation (SPES) at distant cortical sites, in awake human subjects implanted with intracranial electrodes for epilepsy surgery. In 4 out of 5 subjects, the amplitudes of corticocortical evoked potentials (CCEPs) elicited by distant SPES were reproducibly modulated by the power, but not the phase, of local oscillations in alpha and beta frequencies. Specifically, CCEP amplitudes were higher when average oscillatory power just before distant SPES (−110 to −10 ms) was high. This effect was observed in only a subset (0–33%) of sites with CCEPs and, like the CCEPs themselves, varied with stimulation at different distant sites. Our results suggest that although alpha and beta oscillations may gate local processing, they may also enhance the responsiveness of cortex to input from distant cortical sites.

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Design and preparation of efficient, stable and superhydrophobic copper foam membrane for selective oil absorption and consecutive oil–water separation

Publication date: 15 March 2018
Source:Materials & Design, Volume 142
Author(s): Jian Rong, Tao Zhang, Fengxian Qiu, Jicheng Xu, Yao Zhu, Dongya Yang, Yuting Dai
It is extremely important and challenging to develop an effective device for the continuous separation and recovery of large-scale oil–water mixtures due to their contribution to ecological remediation and contamination control. In this work, a superhydrophobic copper foam with high oil–water separation efficiency was successfully fabricated, which could serve both as oil absorption material and oil–water separation membrane, by in situ self-sacrificial template method combining the subsequent steaming modification. The obtained characterization results present that copper foam possesses large pore structure, micro-/nanoscale two-tier surface roughness, high water contact angle (153.6°), and low sliding angle (4.5°). Experimental results show that as-prepared copper foam could not only rapidly absorb residual oil either on the water surface or underwater, but also separate a series of oils from water, like carbon tetrachloride, trichloromethane, methylbenzene, pump oil and diesel. Furthermore, the separation efficiencies of copper foam on trichloromethane-water and diesel-water mixture were maintained above 96.9% and 90.8% after 10 cycles, respectively. Additionally, as-prepared copper foam exhibits excellent corrosion resistant ability and superior hydrophobic stability. The facile, low-cost and controllable strategy presented herein has a bright future in oil–water separation, and also can be further expanded to prepare various anti-corrosion, self-cleaning and water-proof sport materials.

Graphical abstract

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A simple descriptor for energetics at fcc-bcc metal interfaces

Publication date: 15 March 2018
Source:Materials & Design, Volume 142
Author(s): Linda A. Zotti, Stefano Sanvito, David D. O'Regan
We have developed a new and user-friendly interface energy calculation method that avoids problems deriving from numerical differences between bulk and slab calculations, such as the number of k points along the direction perpendicular to the interface. We have applied this to 36 bcc-fcc metal interfaces in the (100) orientation and found a clear dependence of the interface energy on the difference between the work functions of the two metals, on the one hand, and the total number of d electrons on the other. Greater mechanical deformations were observed in fcc crystals than in their bcc counterparts. For each bcc metal, the interface energy was found to follow the position of its d band, whereas the same was not observed for fcc.

Graphical abstract

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Experimental and theoretical analysis of microstructural evolution and deformation behaviors of CuW composites during equal channel angular pressing

Publication date: 15 March 2018
Source:Materials & Design, Volume 142
Author(s): Wenge Chen, Pei Feng, Longlong Dong, Bing Liu, Shuxin Ren, Yongqing Fu
CuW composites were synthesized using an equal channel angular pressing (ECAP) technique. Microstructural evolution during sintering process was investigated using both optical microscopy and transmission electron microscopy (TEM), and their deformation mechanisms were studied using finite element analysis (FEA). Results showed severe plastic deformation of the CuW composites and effective refinement of W grains after the ECAP process. TEM observation revealed that the ECAP process resulted in lamellar bands with high densities dislocations inside the composites. Effects of extrusion temperature and extrusion angles on stress-strain relationship and sizes of deformation zones after the ECAP process were investigated both theoretically and experimentally. When the extrusion angle was 90°, a maximum equivalent stress of ~1001 MPa was obtained when the extrusion test was done at room temperature of 22 °C, and this value was lower than compression strength of the CuW composites (1105.43 MPa). The maximum equivalent strains were varied between 0.5 and 0.7. However, when the extrusion temperature was increased to 550 °C and further to 900 °C, the maximum equivalent stresses were decreased sharply, with readings of 311 MPa and 68 MPa, respectively. When the extrusion angle was increased to 135°, the maximum equivalent stresses were found to be 716.9 MPa, 208 MPa, and 32 MPa for the samples extruded at temperatures of 22 °C, 550 °C and 900 °C, respectively. Simultaneously, the maximum equivalent strains were decreased to 0.2–0.4. Furthermore, results showed that the maximum equivalent stress was located on the sample's external surface and the stress values were gradually decreased from the surface to the center of samples, and the magnitudes of plastic deformation zones at the surface were much larger than those at the central part of the sintered samples. FEA simulation results were in good agreements with experimentally measured ones.

Graphical abstract

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Influence of manganese content on ε-/α′-martensitic transformation and tensile properties of low-C high-Mn TRIP steels

Publication date: 15 March 2018
Source:Materials & Design, Volume 142
Author(s): Xing Li, Liqing Chen, Yang Zhao, Raja Devesh Kumar Misra
Low carbon steels containing 15–19 wt% Mn were processed to study the influence of Mn content on the thermally induced and deformation-induced ε-/α′-martensitic transformation and tensile properties of high-Mn TRIP steels. The stability of austenite and ε-martensite was studied in terms of thermodynamics, and the work hardening behavior during tensile deformation was divided into two stages using Hollomon analysis. The results indicated that Mn increased the stability of austenite and ε-martensite and austenite grain refinement had a larger effect on γ → ε than γ → α′ transformation. During early stages of tensile deformation, the steel having ~15 wt% Mn continued the γ → ε, ε → α′ and γ → α′ transformation because deformation energy compensated the Gibbs free energy required for phase transformation. But α′-martensitic transformation was difficult in steel having ~19 wt% Mn even after fracture because of the high stability of ε-martensite. On account of high density of dislocations in α′-martensite, the dynamic strain aging process was obvious when deformation-induced α′-martensitic transformation occurred in steel. The significant α′-martensitic transformation and intense dynamic strain aging improved work hardening exponent and ultimate tensile strength of steel, while the coordinated transformation of γ → ε and ε → α′ during tensile deformation was beneficial to improve ultimate elongation.

Graphical abstract

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Motor skills intervention research of children with disabilities

Publication date: March 2018
Source:Research in Developmental Disabilities, Volume 74
Author(s): Jason C. Bishop, Melissa Pangelinan
BackgroundPhysical inactivity and obesity among children with physical and cognitive disabilities is an emerging public health issue. Children's motor skill development is a determinant of lifelong physical activity and obesity.AimsThe purpose of this article is to critically evaluate motor skill intervention literature among children with physical and cognitive disabilities.Methods and proceduresElectronic searches were completed to identity research articles published from 1984 to 2014. Major findings were categorized among subtopics including characteristics of intervention studies, research designs, diagnostic method, motor skill interventions and motor skill outcome.Outcomes and results21 studies were found and included participants with developmental delay (42.8%), autism (19.0%), cross-disability (19.0%), intellectual disability (4.8%), cerebral palsy (4.8%), developmental coordination disorder (4.8%), and learning disabilities (4.8%). Only one study was a randomized controlled trial.Conclusionsand implications: The current literature on motor skill intervention research is broad in scope and has limited generalizability within and across disability groups. Future research is needed to develop cross-disability intervention methods adaptable to disability and function-specific needs, including the utilization of rapidly developing technology. Researchers are encouraged to utilize sound methodology with robust theoretical foundations. Family and community engagement is encouraged in intervention delivery.



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