Differential effects of chronic 17β‐estradiol treatment on rat behaviours relevant to depression

Journal of Neuroendocrinology, Volume 0, Issue ja, -Not available-.


https://ift.tt/2IW3640

High‐fat diet and type 2 diabetes induced disruption of the oestrous cycle and alteration of hormonal profiles, but did not affect subpopulations of KNDy neurons in female rats

Journal of Neuroendocrinology, Volume 0, Issue ja, -Not available-.


https://ift.tt/2OnkJ2P

Effect of light-emitting diodes, platelet-rich plasma, and their combination on the activity of sheep tenocytes

Abstract

Healthy tendons play an important role in joint movements and subjected to a group of pathologies called tendinopathy due to multiple factors. Tendons have a slowly repairing process due to the low vascularity and cellularity. Treatment options aimed at potentiating the healing response and relieving symptoms. Phototherapy and platelet-rich plasma were novel treatment modalities in tendons based on photobiomodulation and growth factors during healing, and the results were encouraging suggesting calibrating treatment parameters. This study utilizes cell culture to explore the potential effect of light-emitting diode and/or growth factors in the form of platelet-rich plasma (PRP) on the activity of tenocytes isolated from sheep Achilles tendons by measuring the cell metabolism and cell mobility using cell viability and migration assays to proof safety and confirm activity. Results showed that sheep tenocyte-cultured groups treated with 5% platelet-rich plasma alone or combined with 4 J/cm² light-emitting diode have increased viability significantly when compared to control group after a 48 h, while light-emitting diode treatment has not decreased cell migration significantly when compared with control. Result suggests that using platelet-rich plasma alone or combined with light-emitting diode might have potential to enhance healing response at the conditions applied. PRP could enhance proliferation while LED could enhance migration and proliferation. Further research is needed at longer durations.

https://ift.tt/2NDnNCH

Two cases of erosive pustular dermatosis of the scalp occurring after topical 3,75% imiquimod for actinic keratoses

Dermatologic Therapy, Volume 0, Issue ja, -Not available-.


https://ift.tt/2OTMn74

Psoriasis and Suicidality: A Review of the Literature

Dermatologic Therapy, Volume 0, Issue ja, -Not available-.


https://ift.tt/2PwMwui

Máxime “Max” Kuczyński: Father of Peruvian Public Health and of the 66th President of Peru

Dermatologic Therapy, EarlyView.


https://ift.tt/2OUXi07

Comparison the effects of topical application of olive and calendula ointments on Children's diaper dermatitis: A triple‐blind randomized clinical trial

Dermatologic Therapy, EarlyView.


https://ift.tt/2Pvsy31

Agminated Blue Nevus with a GNAQ mutation: A Case Report and Review of the Literature

Journal of Cutaneous Pathology, Volume 0, Issue ja, -Not available-.


https://ift.tt/2yhxQZv

The expression of terminal deoxynucleotidyl transferase and paired box gene 5 in Merkel cell carcinomas and its relation to the presence of Merkel cell polyomavirus DNA

Journal of Cutaneous Pathology, Volume 0, Issue ja, -Not available-.


https://ift.tt/2yxGTok

Is CD1a useful for Leishmaniasis diagnosis in the new world?

Journal of Cutaneous Pathology, Volume 0, Issue ja, -Not available-.


https://ift.tt/2yfnx8f

Acquired Perforating Calcific Collagenosis in a Drug Addict with Rhabdomyolysis and Transient Hypercalcemia

Journal of Cutaneous Pathology, Volume 0, Issue ja, -Not available-.


https://ift.tt/2yzCl0z

Accuracy of hydrocortisone dose administration via nasogastric tube

Clinical Endocrinology, Volume 0, Issue ja, -Not available-.


https://ift.tt/2OkcaFY

Comparability of dental implant site ridge measurements using ultra-low-dose multidetector row computed tomography combined with filtered back-projection, adaptive statistical iterative reconstruction, and model-based iterative reconstruction

Abstract

Objective

To assess the linear measurements of edentulous ridges recorded from multidetector row computed tomography (MDCT) images obtained by a previously untested ultra-low dose in combination with filtered back-projection (FBP), adaptive statistical iterative reconstruction (ASIR), and model-based iterative reconstruction (MBIR).

Methods

Three cadavers were imaged using a reference protocol with a standard dose and FBP (volume CT dose index (CTDIvol): 29.4 mGy) and two ultra-low-dose protocols, LD1 and LD2 (CTDIvol: 0.53 and 0.29 mGy). All test examinations were reconstructed with FBP, ASIR 50, ASIR 100, and MBIR. Linear measurements from the images of the edentulous ridges recorded from the test protocols were compared with those from the reference using a one-sample t test, Bland–Altman plots, and linear regression. Statistical significance was set at a p value of 0.05.

Results

The one-sample t test demonstrated a statistically significant difference between the measurements from the reference protocol and all test protocols. The difference was not clinically significant for the following three test protocols: LD1/FBP, LD1/ASIR 50, and LD2/FBP. Bland–Altman plots with linear regression showed no systematic variation between the measurements obtained with the reference protocol and these three test protocols.

Conclusions

The lowest-dose protocol to demonstrate comparable measurements with a standard MDCT dose was CTDIvol 0.29 mGy with FBP. These results must be considered with caution for areas of the jaws with thin cortication. The results in areas of thin cortication should be verified by studies with larger sample sizes at such areas and comparison with true gold standard measurements.

https://ift.tt/2ynTU4L

Baclofen-Loaded Poly (d,l-Lactide-Co-Glycolic Acid) Nanoparticles for Neuropathic Pain Management: In Vitro and In Vivo Evaluation

Rejuvenation Research, Ahead of Print.


https://ift.tt/2OXXw6P

Design and Application of 3D-Printed Photometers Controlled with an Arduino

3D Printing and Additive Manufacturing, Ahead of Print.


https://ift.tt/2RLYT75

Claudin‐1 expression decreases with increasing pathological grade in actinic keratosis and may be a marker of high‐risk actinic keratosis

Clinical and Experimental Dermatology, EarlyView.


https://ift.tt/2EiEvrL

2018 Van Meter Lectureship presented by Carmelo Nucera, MD, PhD

2018 Van Meter Lectureship "The role of a new thyroid-specific long non-coding RNA (lincRNA) in drug resistance and iodine metabolism in BRAFV600E thyroid cancer" presented by Carmelo Nucera, MD, PhD

October 11, 2018—The American Thyroid Association (ATA) announces with pleasure that the 2018 Van Meter Award has been presented to Dr. Carmelo Nucera, currently Assistant Professor in the Department of Pathology and at the Cancer Center and Cancer Research Institute of Beth Israel Deaconess Medical Center (BIDMC), Harvard Medical School. He is also an Associate Member at the Broad Institute of Harvard and MIT and Faculty member at the Center for Vascular Biology Research (CVBR) at BIDMC, which is dedicated to "improve human health by using genomics to advance our understanding of the biology and treatment of human disease, and to help lay the groundwork for a new generation of therapies."

The Van Meter Award recognizes outstanding contributions to research on the thyroid gland or related subjects by an investigator who is age 45 or under. Dr. Nucera has given the prestigious Van Meter Lecture on October 4 at 8:05 am, during this year's ATA Annual Meeting in Washington, DC from October 3 to 7. The award winner and the title of his lecture are kept secret until the time of the presentation.  Dr. Nucera spoke on "The role of a new thyroid-specific long non-coding RNA (lincRNA) in drug resistance and iodine metabolism in BRAF^V600E thyroid cancer".

Dr. Nucera was born in Reggio Calabria (Italy), and received his MD summa cum laude in 2000 from the Medical and Surgery School at the University of Messina, Italy, where he also spent his residency in Endocrinology and Metabolic Diseases. During his residency he coordinated a local unit doing ultrasound screening of thyroid nodules, for the Ministry of Italian Scientific and Technological Research. He was also a Research Fellow and Coinvestigator at the Regina Elena Cancer Institute and Gemelli Medical School University Hospital in Rome, where he studied cloning techniques, molecular and cellular biology techniques, and transgenic mouse models for maternal thyroid hormone action during embryo-fetal development. At the Institute of Endocrinology of the University of Catania, he studied DNA automated sequencing and RNA extraction techniques for human thyroid tumors.

Dr. Nucera received his PhD in Experimental Endocrinology and Metabolic Diseases funded through the Italian Ministry of Scientific Research and Education, and completed his thesis on "novel mechanisms of BRAF^V600E-driven thyroid cancer progression" at the Division of Endocrine Surgery at Massachusetts General Hospital (MGH), and at BIDMC at Harvard Medical School.

He was also a post-doctoral research fellow at the same Division at MGH/Harvard. In the years following, Dr. Nucera acted as Attending Physician-Scientist at courses, workshops, and symposia, primarily at Harvard Medical School but also at the ATA.

His academic appointments have included Instructor in the Division of Cancer Biology and Angiogenesis in the Department of Pathology at BIDMC and, for the past five years, Assistant Professor in the Division of Experimental Pathology at the same institution at Harvard Medical School.

Dr. Nucera is primarily engaged in translational thyroid cancer research, as well as in teaching and tutoring medical students, post-docs, PhD students, and College students. He currently focuses on biomarker discovery such as regulatory long intergenic non-coding RNAs (LincRNA) and murine preclinical and co-clinical trials for targeted therapies for super-precision medicine. He is actively developing an independent research program at the BIDMC/Harvard, focused on a preclinical/translational model of patient-derived thyroid cancers and the role of the BRAF^V600E gene mutation in metastatic thyroid cancer. Since his residency, Dr. Nucera has investigated the cellular details of thyroid tumors. His goals have been, among others, to:

• Determine the role of the BRAF gene mutation in human thyroid cancer microenvironment
• Understand the autocrine and paracrine pathways by which BRAF^V600E (serine/threonine-protein kinase) and tyrosine kinase VEGFR2 (one of the vascular endothelial growth factors) promote aggressiveness in papillary thyroid cancer
• Assess the role of stem-cell like pericytes in the mechanisms of drug resistance to targeted therapies in BRAF^V600E thyroid cancer
• Unravel the clonal evolution of BRAF^V600E thyroid cancer treated with targeted therapies

Among the many mentees he has formally supervised are several post-doctoral research fellows, with whom he has collaborated on research studies and papers. For the past decade, Dr. Nucera has given presentations to his medical colleagues, nationally and internationally, concerning the details of his cellular research, thyroid cancer research, tumor modeling, targeted gene therapies, and clinical trials. As principal investigator (PI), he has successfully advanced many basic and clinical-translational projects and collaborations. His research expertise has been enhanced by his teaching experience at Harvard Medical School, resulting in his obtaining major grants as PI from the NIH and National Cancer Institute.

Dr. Nucera has served on the ATA Research and Internet Communications Committees and is a member of the Annual Thyroid Congress Program for the ATA. He has been recently nominated basic research Chair of the ATA Research Committee for 2018.

He is a reviewer for dozens of medical journals, including the New England Journal of Medicine, Frontiers in Oncology, the Journal of the National Cancer Institute, and the ATA's Thyroid. He is an Associate Editor for both Frontiers in Endocrinology and Discoveries. And he has received frequent honors and prizes for his research since medical school.

In his relatively brief career, Dr. Nucera has achieved much more in his research into thyroid oncology and gene therapy than many people over a lifetime. That commitment makes him a very worthy recipient for the prestigious 2018 Van Meter Award from the American Thyroid Association.

 ###

The American Thyroid Association (ATA) is the leading worldwide organization dedicated to the advancement, understanding, prevention, diagnosis, and treatment of thyroid disorders and thyroid cancer. ATA is an international membership medical society with over 1,700 members from 43 countries around the world. Celebrating its 95^th anniversary, the ATA continues to deliver its mission of being devoted to thyroid biology and to the prevention and treatment of thyroid disease through excellence in research, clinical care, education, and public health.  These efforts are carried out via several key endeavors:

•  The publication of the highly regarded professional journals Thyroid, Clinical Thyroidology, and VideoEndocrinology
• Annual scientific meetings
• Biennial clinical and research symposia
• Research grant programs for young investigators
• Support of online professional, public, and patient educational programs
• Development of guidelines for clinical management of thyroid disease and thyroid cancer

 The ATA promotes thyroid awareness and information online through Clinical Thyroidology for the Public and extensive, authoritative explanations of thyroid disease and thyroid cancer in both English and Spanish. The ATA website serves as the clinical resource for patients and the public who look for reliable information on the Internet. Every fifth year, the American Thyroid Association joins with the Latin American Thyroid Society, the European Thyroid Association, and the Asia and Oceania Thyroid Association to cosponsor the International Thyroid Congress (ITC).

The post 2018 Van Meter Lectureship presented by Carmelo Nucera, MD, PhD appeared first on American Thyroid Association.

https://ift.tt/2ISoE1y

Effect of TiF 4 varnish on microbiological changes and caries prevention: in situ and in vivo models

Abstract

Objective

The aim of this study was to evaluate microbiological changes, oral soft tissue toxicity, and caries-preventive effect of an experimental titanium tetrafluoride (TiF₄) varnish compared with a commercially available fluoride varnish (NaF), using in situ and in vivo models.

Materials and methods

The treatment groups were the following: TiF₄ varnish (experimental varnish), Duraphat® (fluoride positive control), placebo varnish (no fluoride), and no treatment (negative control). The varnishes were applied once over the enamel surface using a microbrush. For the in vivo study, 48 Wistar rats were infected with Streptococcus sobrinus 6715, received a treatment, and were submitted to a cariogenic challenge. After 4 weeks, S. sobrinus, oral soft tissue toxicity, presence, and severity of caries were evaluated. For the in situ study, 12 volunteers took part in this randomized crossover, double-blind study performed in four phases of 14 days each. They used intraoral appliances containing four enamel specimens that received the varnish according treatment group. After 24 h, the varnish was removed and plaque accumulation was allowed. A 20% sucrose solution was dripped over the enamel blocks (10×/day for 5 min each). Total streptococci, S. mutans, Lactobacillus, Candida spp. counts, and presence of white spot lesions were evaluated. Lesion depth was also quantified by micro-CT.

Results

For the in vivo study, the fluoride (F-varnishes) showed a statistically significant reduction in the percentage of S. sobrinus compared to the negative control (p < 0.05). Toxicological analysis revealed no abnormalities in oral tissues of rats from all groups, and both F-varnishes reduced the number and severity of caries lesions, without significant differences (p < 0.05). No statistical differences in microbiological counts were seen for the in situ experiment (p > 0.05). However, the specimens treated with TiF₄ exhibited lower percentage of white spot lesions and the lesion depth was significantly reduced by F-varnishes (p < 0.05).

Conclusions

F-varnishes showed reduction in the percentage of S. sobrinus in vivo, no oral soft tissue toxicity, and a caries-preventive effect in vivo and in situ.

Clinical relevance

NaF varnish is largely used due its capacity to form CaF₂-like layer on enamel. Therefore, development of studies focused on other fluoride compounds such as a TiF₄ varnish, which may have greater efficacy than NaF against tooth demineralization, is important.

https://ift.tt/2EeERiY

Penile bulb sparing in prostate cancer radiotherapy

Abstract

Objective

This study aimed to assess the reduction in dose to the penile bulb (PB) achieved by MRI-based contouring following drinking and endorectal balloon (ERB) instructions.

Patients and methods

A total of 17 prostate cancer patients were treated with intensity-modulated radiation therapy (IMRT) and interstitial brachytherapy (IBT). CT and MRI datasets were acquired back-to-back based on a 65 cm³ air-filled ERB and drinking instructions. After rigid co-registration of the imaging data, the CT-based planning target volume (PTV) used for treatment planning was retrospectively compared to an MRI-based adaptive PTV and the dose to the PB was determined in each case. The adapted PTV encompassed a caudally cropped CT-based PTV which was defined on the basis of the MRI-based prostate contour plus an additional 5 mm safety margin.

Results

In the seven-field IMRT treatment plans, the MRI-based adapted PTV achieved mean (D_mean) and maximum (D_max) doses to the PB which were significantly lower (by 7.6 Gy and 10.9 Gy, respectively; p <0.05) than those of the CT-contoured PTV. For 6 patients, the estimated PB D_max (seven-field IMRT and IBT) for the adapted PTV was <70 Gy, whereas only 1 patient fulfilled this criterium with the CT-based PTV.

Conclusion

MRI-based contouring and seven-field IMRT-based treatment planning achieved dose sparing to the PB. Whereas the comparison of MRI and CT contouring only relates to external beam radiotherapy (EBRT) sparing, considering EBRT and IBT shows the improvement in PB sparing for the total treatment.

https://ift.tt/2IRsIzg

Dental radiographic findings in 18 individuals with SATB2 -associated syndrome

Abstract

Objective

To characterize the radiographic dental phenotype of individuals with SATB2-associated syndrome (SAS).

Materials and methods

Participants were evaluated by a multidisciplinary team during a concurrent clinic conducted during the 1st international SAS family meeting held in 2017 at a single institution. Whenever possible, panoramic and/or periapical radiographs were obtained in clinic or previously obtained and provided by the caregiver.

Results

Of the 37 individuals evaluated, 18 (12 males, median age 8.5 years) underwent radiographic examination. Dental radiographs revealed anomalies in all individuals starting at 2 years of age. The most consistent finding was delayed development of the mandibular second bicuspids (83%) with other common radiographic findings including delayed development of the roots of the permanent teeth (78%), severely rotated (56%) or malformed teeth (44%), and taurodontism (44%).

Conclusions

Dental anomalies are fully penetrant and can be documented radiographically in all individuals with SAS.

Clinical relevance

Dental radiographic findings of delayed second premolar development and delayed development of permanent root formation, especially concurrent with findings of taurodontism and malformed teeth, support a clinical suspicion for SAS and should help differentiate SAS from other neurodevelopmental syndromes.

https://ift.tt/2QLYeRM