Calcium Imaging Reveals Coordinated Simple Spike Pauses in Populations of Cerebellar Purkinje Cells

Publication date: 20 December 2016
Source:Cell Reports, Volume 17, Issue 12
Author(s): Jorge E. Ramirez, Brandon M. Stell
The brain's control of movement is thought to involve coordinated activity between cerebellar Purkinje cells. The results reported here demonstrate that somatic Ca²⁺ imaging is a faithful reporter of Na⁺-dependent "simple spike" pauses and enables us to optically record changes in firing rates in populations of Purkinje cells in brain slices and in vivo. This simultaneous calcium imaging of populations of Purkinje cells reveals a striking spatial organization of pauses in Purkinje cell activity between neighboring cells. The source of this organization is shown to be the presynaptic gamma-Aminobutyric acid producing (GABAergic) network, and blocking ionotropic gamma-Aminobutyric acid receptor (GABAARs) abolishes the synchrony. These data suggest that presynaptic interneurons synchronize (in)activity between neighboring Purkinje cells, and thereby maximize their effect on downstream targets in the deep cerebellar nuclei.

Graphical abstract

Teaser

Ramirez and Stell use high-speed calcium imaging to monitor action potentials in populations of neurons and extract pauses in ongoing neuronal activity as well as changes in spiking rate. They see that presynaptic GABAergic interneurons organize Purkinje cell activity by simultaneously pausing spiking in neighboring Purkinje cells.


http://ift.tt/2hK6eEQ