Colorimetric semi-quantitative measurement of pyrophosphate by functionalised SPPS resin in biological media

Publication date: May 2017
Source:Sensors and Actuators B: Chemical, Volume 243
Author(s): Benjamin M. Long, Frederick M. Pfeffer, Colin J. Barrow
Inorganic pyrophosphate is a molecule of significant biological importance, which has roles in metabolism and disease diagnosis. In this communication we demonstrate a novel platform for the colorimetric naked eye detection of pyrophosphate using a threshold test in both aqueous solutions and biological fluids. Our system uses peptide synthesis resin as a solid support for a bis-Zn(II)dipicolylamino mediated indicator displacement assay. Our threshold tests are able to detect pyrophosphate in concentrations as low as 1mM.

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