Kanamycin detection based on the catalytic ability enhancement of gold nanoparticles

Publication date: 15 May 2017
Source:Biosensors and Bioelectronics, Volume 91
Author(s): Chengke Wang, Dan Chen, Qingqing Wang, Rong Tan
In this paper, we demonstrated that kanamycin could enhance the peroxidase-like activity of citrate-capped gold nanoparticles (AuNPs) through two steps: the attachment of kanamycin onto AuNPs through −NH2 (on kanamycin) and −COOH (on AuNPs) interactions; and the specifically interaction between glucoside on kanamycin and AuNPs which changes the surface property of AuNPs, and produced •OH radicals and Au³⁺ in the solution, and catalyzed the chromogenic reactions between 3, 3′, 5, 5′-tetramethylbenzidine (TMB) and H2O2. Based on this principle, a novel method for kanamycin detection has been developed. This method exhibited high sensitivity and selectivity, as low as 0.1nM kanamycin could be detected with a linear range from 0.1nM to 20nM and 20nM to 300nM, respectively. This method was also successfully applied for the detection of kanamycin content in milk and meat samples.



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