An integrative analysis of DNA methylation in osteosarcoma

Publication date: Available online 9 January 2017
Source:Pathology - Research and Practice
Author(s): Baohua Huang, Jiangdong Du, Qing Lin, Limei Yu, Liping Yang, Chengming Sun, Jie Li, Xia Zhang
BackgroundThe study aimed to analyze aberrantly methylated genes, relevant pathways and transcription factors (TFs) in osteosarcoma (OS) development.MethodsBased on the DNA methylation microarray data GSE36002 that were downloaded from GEO database, the differentially methylated genes in promoter regions were identified between OS and normal samples. Pathway and function enrichment analysis of differentially methylated genes was performed. Subsequently, protein-protein interaction (PPI) network was constructed, followed by identification of cancer-associated differentially methylated genes and significant differentially methylated TFs.ResultsA total of 1379 hyper-methylation regions and 169 hypo-methylation regions were identified in OS samples compared to normal samples. The differentially hyper-methylated genes were significantly enriched in Neuroactive ligand-receptor interaction pathway and PPAR signaling pathway. The differentially hypo-methylated genes were significantly enriched in toll-like receptor signaling pathway. In PPI network, signal transducers and activators of transcription (STAT3) had high degree (degree=21). MAX interactor 1, dimerization protein (MXI1), STAT3 and T-cell acute lymphocytic leukemia 1 (TAL1) were significant TFs enriched with target genes in OS samples. They were found to be cancer-associated and hyper-methylated in OS samples.ConclusionNeuroactive ligand-receptor interaction, PPAR signaling, and toll-like receptor signaling pathways are implicated in OS. MXI1, STAT3, and TAL1 may be important TFs in OS development.



http://ift.tt/2jptLbB