Infrared and Raman Microscopy and Imaging of Biomaterials at the Micro and Nano Scale

Publication date: 2017
Source:Reference Module in Materials Science and Materials Engineering
Author(s): C. Marcott, M. Padalkar, N. Pleshko
Fourier transform infrared (FTIR) and Raman spectroscopy have been widely utilized to evaluate the molecular structure of polymers, biomaterials, and tissues, with the choice of technique dependent on the problem under investigation. Major advances in spectroscopic analyses occurred with the coupling of microscopes to infrared and Raman spectrometers, developments that allowed investigators to assess the location of different chemical species at high spatial resolution, on the order of 1µm for Raman, and 2–6µm for FTIR. Further advances were the development of imaging technology, where an array detector is utilized to efficiently collect high-resolution spectral data from relatively large sections (millimeters) of tissue and material samples, and most recently, the development of nanoscale infrared spectroscopy, known as atomic force microscopy-infrared (AFM-IR). Several applications to biomaterials are discussed here, including assessment of polymer composition and degradation, evaluation of composition of tissue-engineered constructs and cells, of dental materials, and characterization of drug delivery systems.



http://ift.tt/2jnXjdY