Rapid evaporative ionization mass spectrometry for high-throughput screening in food analysis: The case of boar taint

Publication date: 1 July 2017
Source:Talanta, Volume 169
Author(s): Kaat Verplanken, Sara Stead, Renata Jandova, Christof Van Poucke, Jan Claereboudt, Julie Vanden Bussche, Sarah De Saeger, Zoltan Takats, Jella Wauters, Lynn Vanhaecke
Boar taint is a contemporary off-odor present in meat of uncastrated male pigs. As European Member States intend to abandon surgical castration of pigs by 2018, this off-odor has gained a lot of research interest. In this study, rapid evaporative ionization mass spectrometry (REIMS) was explored for the rapid detection of boar taint in neck fat. Untargeted screening of samples (n=150) enabled discrimination between sow, tainted and untainted boars. The obtained OPLS-DA models showed excellent classification accuracy, i.e. 99% and 100% for sow and boar samples or solely boar samples, respectively. Furthermore, the obtained models demonstrated excellent validation characteristics (R²(Y)=0.872–0.969; Q²(Y)=0.756–0.917), which were confirmed by CV-ANOVA (p<0.001) and permutation testing. In conclusion, in this work for the first time highly accurate and high-throughput (<10s) classification of tainted and untainted boar samples was achieved, rendering REIMS a promising technique for predictive modelling in food safety and quality applications.

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