Oxidative and cellular stress as bioindicators for metal contamination in freshwater mollusk Lamellidens marginalis

Abstract

The concentrations of 14 metals, namely, chromium (Cr), manganese (Mn), cobalt (Co), nickel (Ni), copper (Cu), zinc (Zn), selenium (Se), arsenic (As), strontium (Sr), cadmium (Cd), tin (Sn), antimony (Sb), mercury, (Hg), and lead (Pb), have been studied in freshwater mollusk Lamellidens marginalis collected from Dhimbe reservoir. In addition, also studied the cellular and antioxidative status to assess metal contamination as pollution biomarkers. The levels of all the 14 metals were elevated in different sampling sites of Dhimbe reservoir. The level of metals in the L. marginalis was followed as Mn > Zn > Ni > Sr > Cr > Cu > Hg > Se > Sn > Pb > As > Co > Cd > Sb. The oxidative biomarkers viz. catalase (CAT), superoxide dismutase (SOD), and glutathione-S-transferase (GST); glycolytic enzymes viz. lactate dehydrogenase (LDH) and malate dehydrogenase (MDH); and protein metabolic enzymes viz. aspartate amino transferase (AST), alanine amino transferase (ALT), and lipid peroxidation (LPO) were significantly (p < 0.01) higher, while neurotransmitter enzymes such as acetylcholine esterase (AChE) were significantly inhibited in muscle of L. marginalis (p < 0.01) collected from different sampling sites. Therefore, based on our results, we could recommend that the Dhimbe reservoir is moderately metal contaminated and investigation based on biochemical parameters such as antioxidative enzymes, cellular biomarkers, and AChE are strong indicators for metal contamination; hence, these parameters are successfully applied as reliable biomarkers for monitoring contaminated freshwater ecosystems.

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