Abstract
With the aim of studying delta-like protein 1 (DLK1) implication in the relationship between adipocyte leptin and adenohypophyseal hormones, we carried out an immunohistochemical study to analyse the presence of receptors for these hormones in pituitary and adipose cells of male wildtype mice (Dlk1+/+), compared to knockout mice (Dlk1-/-). The mRNA expression of these molecules was also determined by RT-PCR. Our results showed that in wildtype adipose cells all the adenohypophyseal hormone receptors were present, with a higher mRNA expression for GHR and TSHR. Of the total cells in the anterior pituitary lobe, 17.09 ± 0.9% were leptin-receptor (LEPR) immunoreactive (-ir), mainly in GH-ir and PRL-ir cells (41.5 ± 3.8%; 13.5 ± 1.7%, respectively). In Dlk1-/- mice, adipocyte cells showed a significant increase in the TSHR mRNA expression level. Moreover, the percentage of LEPR-ir GH cells showed a statistically significant increase compared to controls, from 41.5± 3.8% to 53.1± 4.0%. In contrast, only 3.0 ± 0.6% of LEP-ir anterior pituitary cells were detected in Dlk1 knockout mice, opposed to 6.8± 1.1% observed in WT mice. The results suggest relationships between adipocytes and pituitary GH, PRL and TSH cells, in addition to influencing the synthesis and release of pituitary leptin, particularly in PRL cells.
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