Dihydropyridine-derived BODIPY probe for detecting exogenous and endogenous nitric oxide in mitochondria

Publication date: 1 January 2018
Source:Talanta, Volume 176
Author(s): Chang Gao, Lan Lin, Wan Sun, Zheng-Li Tan, Jun-Ru Huang, Lan He, Zhong-Lin Lu
A mitochondria-targetable probe Mito-DHP for nitric oxide (NO) was designed and synthesized by introducing dihydropyridine and triphenylphosphonium (TPP) moieties into boron dipyrromethene (BODIPY) dye. Mito-DHP was able to effectively detect nitric oxide through the aromatization of dihydropyridine to fluorescent pyridine product under oxygen-free conditions. The probe Mito-DHP showed high selectivity to NO over a number of reactive oxygen/nitrogen species (ROS/RNS) as well as high sensitivity (detection limit at 25nM), pH stability and bio-compatibility. Furthermore, Mito-DHP proved to target mitochondria specifically and to visualize both exogenous and endogenous NO in real time.

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