Hyperaccumulating potential of Bidens pilosa L. for Cd and elucidation of its translocation behavior based on cell membrane permeability

Abstract

Phytoremediation with the use of hyperaccumulating plant species to remove excess trace metals from contaminated soil and water is considered a cost-effective non-invasive technique. Over 400 plant taxa worldwide have been identified as natural hyperaccumulators, but only very few are reported to hyperaccumulate Cd. Bidens pilosa L. is a newly found, promising Cd hyperaccumulator, although its potential to accumulate Cd and mechanism of this process are not yet well known. This paper was aimed at exploring hyperaccumulation capacity of B. pilosa for Cd, and its translocation behavior related to cell membrane permeability. The highest Cd concentration in shoots of B. pilosa grown in soil was 405.91 mg kg⁻¹ and of that cultured in nutrient solution 1651.68 mg kg⁻¹, indicating very high accumulation potential. Cd concentrations in the root, stem, leaf, and shoot of B. pilosa cultured in nutrient solution were all much higher than those in soil, while biomass development was considerably lower. This resulted in lesser differences between Cd maximum accumulation loads in the shoot (462 and 365 μg pot⁻¹) and in the root (100 and 96 μg pot⁻¹) of B. pilosa grown in solution and in soil, respectively. Relative electric conductivity (REC), K⁺ relative permeability ratio, and MDA (malondialdehyde) contents, which are major indices expressing cell membrane permeability, appeared to be closely related to Cd translocation and accumulation. The relative molecular mechanism of Cd accumulation/translocation in B. pilosa was found of importance and needs to be elucidated.

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