Drug release, cytocompatibility, bioactivity, and antibacterial activity of doxycycline loaded Mg-Ca-TiO2 composite scaffold

Publication date: 5 February 2018
Source:Materials & Design, Volume 139
Author(s): H.R. Bakhsheshi-Rad, E. Hamzah, Mark P. Staiger, George J. Dias, Z. Hadisi, M. Saheban, M. Kashefian
Mg-Ca-TiO2 (MCT) composite scaffolds loaded with different concentrations of doxycycline (DC) with a network of interconnected pores with good compressive strength (5±0.1MPa) were fabricated via space holder method for the first time. The results showed that MCT-DC scaffolds possess a porosity and pore size in the range of 65–67% and 600–800μm respectively. The bioactivity results exhibited the apatite formation on the MCT-DC scaffold surface, indicating that DC did not obstruct the bioactivity of MCT. The MCT-DC scaffolds drug release profiles show the initial burst and sustained drug release (55–75%) and the release rate could be adjusted via altering the DC concentration. The MCT loaded with 1 and 5% DC did not indicate cytotoxic behavior against MG63 cells while further DC loading resulted in some toxicity. Antimicrobial properties of MCT-DC scaffolds against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) bacteria were examined and the results reveal oblivious inhibition zone around each MCT-DC scaffold whereas no obvious inhibition is observed around the MCT scaffold. Therefore, MCT-DC composite scaffolds with low concentration of DC could be alternative candidates for infection prevention and bone tissue engineering.

Graphical abstract

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