Photodynamic Diagnosis of Parathyroid Glands with Nano-stealth Aminolevulinic Acid Liposomes

Publication date: Available online 15 November 2017
Source:Photodiagnosis and Photodynamic Therapy
Author(s): Shaimaa Elbassiouny, Maha Fadel, Tarek Elwakeel, Mahmoud Saber
Aim of the study: Exploring whether the formulation of ALA in nano-stealth liposomes would inflict its photodiagnostic effectiveness.The present study represents ALA in nano-stealth liposomes as well as in conventional nanoliposomes in order to be used for photodiagnosis of parathyroid glands intraoperatively. Materials and Methods: Preparation of ALA nanovesicles and characterization for the drug encapsulation%, vesicle size, Zeta potential, in vitro drug release profile and stability. Subsequently, intraperitoneal administration of ALA in rats and study the feasibility and efficacy of these formulations in identifying parathyroids intraoperatively by a fluorescence technique and evaluation of fluorescence differences between these delivery forms and the free ALA solution used earlier. Results and conclusion: Stealth liposomes were insignificantly higher in drug encapsulation%, in vitro drug release and zeta potential compared to conventional liposomes. Additionally, they needed less time for the start of the photosensitization and recorded the highest signal after spectrometry compared to the other two preparations. These data provide a new evidence of the potentiality of ALA-stealth Liposomes for identification of PTGs intraoperatively and could lead to propose a non-invasive procedure with reduced postoperative side effects.



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