Ετικέτες

Παρασκευή 12 Ιανουαρίου 2018

Ultrasensitive electrochemical immuno-sensing platform based on gold nanoparticles triggering chlorpyrifos detection in fruits and vegetables

S09565663.gif

Publication date: 15 May 2018
Source:Biosensors and Bioelectronics, Volume 105
Author(s): Anita Talan, Annu Mishra, Sergei A. Eremin, Jagriti Narang, Ashok Kumar, Sonu Gandhi
Chlorpyrifos (chl) is an organophosphate pesticide extensively used in agriculture and highly toxic for human health. Fluorine doped tin-oxide (FTO) based electrochemical nanosensor was developed for chlorpyrifos detection with gold nanoparticles (AuNPs) and anti-chlorpyrifos antibodies (chl-Ab). AuNPs provides high electrical conductivity and specific resistivity, thus increases the sensitivity of immunoassay. High electrical conductivity of AuNPs reveals that it promotes the redox reaction for better cyclic voltammetry. Based on the intrinsic conductive properties of FTO-AuNPs complex, chl-Ab was immobilized onto AuNPs surface. Under optimized conditions, the proposed FTO based nanosensor exhibited high sensitivity and stable response for the detection of chlorpyrifos, ranging from 1fM to 1µM with limit of detection (LOD) up to 10fM. The FTO-AuNPs sensor was successfully employed for the detection of chlorpyrifos in standard as well in real samples up to 10nM for apple and cabbage, 50nM for pomegranate. The proposed FTO-AuNPs nanosensor can be used as a quantitative tool for rapid, on-site detection of chlorpyrifos traces in real samples when miniaturized due to its excellent stability, sensitivity, and simplicity.



http://ift.tt/2D8oO4F

Δεν υπάρχουν σχόλια:

Δημοσίευση σχολίου

Αναζήτηση αυτού του ιστολογίου