DJ-1 is a useful biomarker for invasive extrahepatic cholangiocarcinoma

Publication date: Available online 13 February 2018
Source:Human Pathology
Author(s): Yukiko Tabata, Yoshitsugu Nakanishi, Kanako C. Hatanaka, Yutaka Hatanaka, Takahiro Tsuchikawa, Keisuke Okamura, Takehiro Noji, Toshiaki Shichinohe, Yoshihiro Matsuno, Satoshi Hirano
We have previously reported that DJ-1 protein is up-regulated in cholangiocarcinoma compared with non-neoplastic epithelium of the bile duct in a study using liquid chromatography mass spectrometry-based proteomics. The aim of this study was to clarify whether DJ-1 expression offers a biomarker for patients with invasive extrahepatic cholangiocarcinoma (EHCC) who undergo surgical resection with curative intent. Positive immunohistochemical (IHC) staining of DJ-1 was significantly more frequent in the cytoplasm of 96 invasive EHCCs (n=28, 29.2%) than in that of 66 non-neoplastic epithelial lesions adjacent to invasive EHCC (n=7, 10.6%; P=.006). No significant difference in clinicopathological features was evident between invasive EHCC patients with negative (n=68) and positive (n=28) IHC staining. However, negative IHC staining for DJ-1 in cytoplasm was selected as an independent risk factor for adverse prognosis on multivariate analysis (P=.004, hazard ratio 2.13, 95% confidence interval 1.28–3.57). Serum levels of DJ-1 in 16 invasive EHCC patients with metastasis were compared with 12 invasive EHCC patients without metastasis. Serum levels of DJ-1 tended to be higher in 16 patients with metastasis (median, 40.9ng/ml) than in 12 patients without (27.6ng/ml, P=.137). In addition, patients with high serum levels (≥ 40ng/ml) of DJ-1 tended to have metastasis more frequently than those without (P=.054, Fisher's exact test). We concluded that IHC staining pattern and serum level of DJ-1 in patients with invasive EHCC might be predictive of prognosis and metastasis, respectively.



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