Circulating CD14+CD163+CD115+ M2 monocytes are associated with the severity of new onset severe acute pancreatitis in Chinese patients

Publication date: April 2018
Source:International Immunopharmacology, Volume 57
Author(s): Manli Zhang, Lili Ding, Xinrui Wang, Jie Hou, Man Li, Yanfang Jiang, Xiuting He, Meizi Cui, Feng Hu, Xiuna Zhang, Jingyuan Yang, Xiaohe Guo, Huiying Zhao, Pujun Gao
BackgroundDespite the role of monocytes in the pathogenesis of severe acute pancreatitis (SAP), it remains unclear how different subtypes of monocytes regulate and contribute to this pathogenesis.MethodsWe examined the numbers of different subsets of monocytes by flow cytometry in 21 SAP, 15 mild acute pancreatitis (MAP) and 13 healthy controls (HC). The concentrations of plasma cytokines were assessed by cytometric bead array. Disease severity was evaluated based on the acute physiology and chronic health evaluation (APACHE) II score and plasma C-reactive proteins (CRP) levels.ResultsCompared with the numbers in MAP patients and HC, we observed that the numbers of CD14⁺CD163⁻, CD14⁺CD163⁻MAC387⁺, CD14⁺CD163⁻IL-12⁺ M1 monocytes, and CD115⁺, CD204⁺, IL-10⁺ M2 monocytes were significantly increased in SAP patients. In addition, these patients showed higher plasma levels of interleukin (IL)-12 and IL-10. Furthermore, the number of CD14⁺CD163⁻, CD14⁺CD163⁻MAC387⁺ M1 monocytes and the plasma IL-12 concentration showed a positive association with the CRP level, while the number of CD204⁺, IL-10⁺ M2 monocytes and the plasma IL-10 concentration showed a positive correlation with the APACHE II score. Importantly, the CD115⁺ M2 subset displayed a positive correlation with both the CRP level and APACHE II score, and treatment of SAP significantly reduced the number of this subset.ConclusionsThe CD14⁺CD163⁺CD115⁺ M2 monocyte count appears to be important factor in determining the severity and prognosis of SAP. Both the pro- and anti-inflammatory monocytes appear to participate in the pathogenesis of SAP.



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