Mannosyl electrochemical impedance cytosensor for label-free MDA-MB-231 cancer cell detection

Publication date: 30 September 2018
Source:Biosensors and Bioelectronics, Volume 116
Author(s): Yi-Hsuan Tang, Han-Chen Lin, Chiao-Ling Lai, Po-Yu Chen, Chian-Hui Lai
A label-free and ultrasensitive electrochemical impedance cytosensor was developed to specifically detect the breast cancer cells MDA-MB-231 via the interaction between the mannosyl glassy carbon electrode (GCE) and the overexpressed mannose receptors on the target cell surface. The mannosyl GCE was prepared through electrografting of the amino-functionalized mannose derivatives on GCE surface in which a covalent bond was formed between carbon of the electrode and the amino group of the mannose derivative. The fluorescent microscopy indicated that the electrode is specific for MDA-MB-231 cells, with good biocompatibility for viable captured cells. The derivative with a shorter alkyl linker, mannose-C2NH2, showed a better sensitivity than that with a longer linker, mannose-C6NH2. GCE modified with amino-functionalized galactose derivative, galactose-C2NH2, shows no function to the detection of MDA-MB-231 cells. The specific interaction between the mannosyl GCE and Con A (a mannose-binding lectin) or MDA-MB-231 breast cancer cells with overexpressed mannose receptors was determined through the change of peak separation in the cyclic voltammogram or the change of charge transfer resistance in the electrochemical impedance spectra (Nyquist plot) in the electrolytes containing a reversible redox couple [Fe(CN)6]³⁻/[Fe(CN)6]⁴⁻. The charge transfer resistance in the Nyquist plots linearly depended on the concentration of MDA-MB-231 cells (1.0 × 10–1.0 × 10⁵ cells mL⁻¹, with 10 cells mL⁻¹ being the lower detection limit). Introducing 0.1% polyethylene glycol-200 (PEG-200) was able to prevent the interference caused by 1.0 × 10³ HEK-293T cells mL⁻¹, a non-cancer cell line (control).

Graphical abstract

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Ultrasensitive dual probe immunosensor for the monitoring of nicotine induced-brain derived neurotrophic factor released from cancer cells

Publication date: 30 September 2018
Source:Biosensors and Bioelectronics, Volume 116
Author(s): Mahmood H. Akhtar, Khalil K. Hussain, N.G. Gurudatt, Pranjal Chandra, Yoon-Bo Shim
Brain-derived neurotrophic factor (BDNF) was detected in the extracellular matrix of neuronal cells using a dual probe immunosensor (DPI), where one of them was used as a working and another bioconjugate loading probe. The working probe was fabricated by covalently immobilizing capture anti-BDNF (Cap Ab) on the gold nanoparticles (AuNPs)/conducting polymer composite layer. The bioconjugate probe was modified by drop casting a bioconjugate particles composed of conducting polymer self-assembled AuNPs, immobilized with detection anti-BDNF (Det Ab) and toluidine blue O (TBO). Each sensor layer was characterized using the surface analysis and electrochemical methods. Two modified probes were precisely faced each other to form a microfluidic channel structure and the gap between inside modified surfaces was about 19 µm. At optimized conditions, the DPI showed a linear dynamic range from 4.0 to 600.0 pg/ml with a detection limit of 1.5 ± 0.012 pg/ml. Interference effect of IgG, arginine, glutamine, serine, albumin, and fibrinogene were examined and stability of the developed biosensor was also investigated. The reliability of the DPI sensor was evaluated by monitoring the extracellular release of BDNF using exogenic activators (ethanol, K⁺, and nicotine) in neuronal and non-neuronal cells. In addition, the effect of nicotine onto neuroblastoma cancer cells (SH-SY5Y) was studied in detail.



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HER1-based vaccine: simultaneous activation of humoral and cellular immune response

Publication date: Available online 31 May 2018
Source:Seminars in Oncology
Author(s): Gretchen Bergado Báez, Diana R. Hernández Fernández, Zaima Mazorra Herrera, Belinda Sánchez Ramírez
The human epidermal growth factor receptor 1 (HER1) is a tumor associated antigen that has been validated as a clinical target for several passive, non-immune therapies currently approved for the treatment epithelial tumors. HER1 is an oncogene that not only promotes tumor progression and survival, but also immune-escape. Its overexpression in some epithelial malignancies has been correlated with a poor prognosis. We developed an approach to target HER1 by specific active immunotherapy, recognizing the extracellular domain of the receptor, using a combination of VSSP and Montanide ISA 51 as adjuvants. We summarize the results obtained with this vaccine in both the preclinical and clinical settings, emphasizing the importance of the induction of both humoral and cellular responses for the success of cancer vaccines, as safe therapeutic alternatives for the treatment of cancer.



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Molecular mechanisms underlying the neuroprotective role of atrial natriuretic peptide in experimental acute ischemic stroke

Publication date: 5 September 2018
Source:Molecular and Cellular Endocrinology, Volume 472
Author(s): Mikahela A. López-Morales, María Castelló-Ruiz, María C. Burguete, Teresa Jover-Mengual, Alicia Aliena-Valero, José M. Centeno, Enrique Alborch, Juan B. Salom, Germán Torregrosa, Francisco J. Miranda
Along with its role in regulating blood pressure and fluid homeostasis, the natriuretic peptide system could be also part of an endogenous protective mechanism against brain damage. We aimed to assess the possibility that exogenous atrial natriuretic peptide (ANP) could protect against acute ischemic stroke, as well as the molecular mechanisms involved. Three groups of rats subjected to transient middle cerebral artery occlusion (tMCAO, intraluminal filament technique, 60 min) received intracerebroventricular vehicle, low-dose ANP (0.5 nmol) or high-dose ANP (2.5 nmol), at 30 min reperfusion. Neurofunctional condition, and brain infarct and edema volumes were measured at 24 h after tMCAO. Apoptotic cell death and expression of natriuretic peptide receptors (NPR-A and NPR-C), K⁺ channels (KATP, KV and BKCa), and PI3K/Akt and MAPK/ERK1/2 signaling pathways were analyzed. Significant improvement in neurofunctional status, associated to reduction in infarct and edema volumes, was shown in the high-dose ANP group. As to the molecular mechanisms analyzed, high-dose ANP: 1) reduced caspase-3-mediated apoptosis; 2) did not modify the expression of NPR-A and NPR-C, which had been downregulated by the ischemic insult; 3) induced a significant reversion of ischemia-downregulated KATP channel expression; and 4) induced a significant reversion of ischemia-upregulated pERK2/ERK2 expression ratio. In conclusion, ANP exerts a significant protective role in terms of both improvement of neurofunctional status and reduction in infarct volume. Modulation of ANP on some molecular mechanisms involved in ischemia-induced apoptotic cell death (KATP channels and MAPK/ERK1/2 signaling pathway) could account, at least in part, for its beneficial effect. Therefore, ANP should be considered as a potential adjunctive neuroprotective agent improving stroke outcome after successful reperfusion interventions.



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Cardiolipin Synthesis in Brown and Beige Fat Mitochondria Is Essential for Systemic Energy Homeostasis

Publication date: Available online 31 May 2018
Source:Cell Metabolism
Author(s): Elahu G. Sustarsic, Tao Ma, Matthew D. Lynes, Michael Larsen, Iuliia Karavaeva, Jesper F. Havelund, Carsten H. Nielsen, Mark P. Jedrychowski, Marta Moreno-Torres, Morten Lundh, Kaja Plucinska, Naja Z. Jespersen, Trisha J. Grevengoed, Barbara Kramar, Julia Peics, Jakob B. Hansen, Farnaz Shamsi, Isabel Forss, Ditte Neess, Susanne Keipert, Jianing Wang, Katharina Stohlmann, Ivan Brandslund, Cramer Christensen, Marit E. Jørgensen, Allan Linneberg, Oluf Pedersen, Michael A. Kiebish, Klaus Qvortrup, Xianlin Han, Bente Klarlund Pedersen, Martin Jastroch, Susanne Mandrup, Andreas Kjær, Steven P. Gygi, Torben Hansen, Matthew P. Gillum, Niels Grarup, Brice Emanuelli, Søren Nielsen, Camilla Scheele, Yu-Hua Tseng, Nils J. Færgeman, Zachary Gerhart-Hines
Activation of energy expenditure in thermogenic fat is a promising strategy to improve metabolic health, yet the dynamic processes that evoke this response are poorly understood. Here we show that synthesis of the mitochondrial phospholipid cardiolipin is indispensable for stimulating and sustaining thermogenic fat function. Cardiolipin biosynthesis is robustly induced in brown and beige adipose upon cold exposure. Mimicking this response through overexpression of cardiolipin synthase (Crls1) enhances energy consumption in mouse and human adipocytes. Crls1 deficiency in thermogenic adipocytes diminishes inducible mitochondrial uncoupling and elicits a nuclear transcriptional response through endoplasmic reticulum stress-mediated retrograde communication. Cardiolipin depletion in brown and beige fat abolishes adipose thermogenesis and glucose uptake, which renders animals insulin resistant. We further identify a rare human CRLS1 variant associated with insulin resistance and show that adipose CRLS1 levels positively correlate with insulin sensitivity. Thus, adipose cardiolipin has a powerful impact on organismal energy homeostasis through thermogenic fat bioenergetics.

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Teaser

Sustarsic et al. reveal that synthesis of the mitochondrial phospholipid cardiolipin is a hallmark of brown and beige fat activation by cold temperature. This single lipid species in thermogenic fat not only shapes adipose mitochondrial bioenergetics but also exerts profound control over whole-body insulin sensitivity and metabolic flexibility.


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Ubiquitination of ABCE1 by NOT4 in Response to Mitochondrial Damage Links Co-translational Quality Control to PINK1-Directed Mitophagy

Publication date: Available online 31 May 2018
Source:Cell Metabolism
Author(s): Zhihao Wu, Yan Wang, Junghyun Lim, Boxiang Liu, Yanping Li, Rasika Vartak, Trisha Stankiewicz, Stephen Montgomery, Bingwei Lu
Translation of mRNAs is tightly regulated and constantly surveyed for errors. Aberrant translation can trigger co-translational protein and RNA quality control processes, impairments of which cause neurodegeneration by still poorly understood mechanism(s). Here we show that quality control of translation of mitochondrial outer membrane (MOM)-localized mRNA intersects with the turnover of damaged mitochondria, both orchestrated by the mitochondrial kinase PINK1. Mitochondrial damage causes stalled translation of complex-I 30 kDa subunit (C-I30) mRNA on MOM, triggering the recruitment of co-translational quality control factors Pelo, ABCE1, and NOT4 to the ribosome/mRNA-ribonucleoprotein complex. Damage-induced ubiquitination of ABCE1 by NOT4 generates poly-ubiquitin signals that attract autophagy receptors to MOM to initiate mitophagy. In the Drosophila PINK1 model, these factors act synergistically to restore mitophagy and neuromuscular tissue integrity. Thus ribosome-associated co-translational quality control generates an early signal to trigger mitophagy. Our results have broad therapeutic implications for the understanding and treatment of neurodegenerative diseases.

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Removal of damaged mitochondria is essential for maintaining cellular vitality, but the earliest signal that initiates the mitophagy process is not well defined. Wu et al. show that mitochondrial damage causes stalled translation of OXPHOS-related mRNAs on the mitochondrial surface. Co-translational quality control of stalled ribosomes generates ubiquitin-containing signals that trigger mitophagy.


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Chrono-pharmacological Targeting of the CCL2-CCR2 Axis Ameliorates Atherosclerosis

Publication date: Available online 31 May 2018
Source:Cell Metabolism
Author(s): Carla Winter, Carlos Silvestre-Roig, Almudena Ortega-Gomez, Patricia Lemnitzer, Hessel Poelman, Ariane Schumski, Janine Winter, Maik Drechsler, Renske de Jong, Roland Immler, Markus Sperandio, Michael Hristov, Tanja Zeller, Gerry A.F. Nicolaes, Christian Weber, Joana R. Viola, Andres Hidalgo, Christoph Scheiermann, Oliver Soehnlein
Onset of cardiovascular complications as a consequence of atherosclerosis exhibits a circadian incidence with a peak in the morning hours. Although development of atherosclerosis extends for long periods of time through arterial leukocyte recruitment, we hypothesized that discrete diurnal invasion of the arterial wall could sustain atherogenic growth. Here, we show that myeloid cell recruitment to atherosclerotic lesions oscillates with a peak during the transition from the activity to the resting phase. This diurnal phenotype is regulated by rhythmic release of myeloid cell-derived CCL2, and blockade of its signaling abolished oscillatory leukocyte adhesion. In contrast, we show that myeloid cell adhesion to microvascular beds peaks during the early activity phase. Consequently, timed pharmacological CCR2 neutralization during the activity phase caused inhibition of atherosclerosis without disturbing microvascular recruitment. These findings demonstrate that chronic inflammation of large vessels feeds on rhythmic myeloid cell recruitment, and lay the foundation for chrono-pharmacology-based therapy.

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Teaser

Winter et al. identify an oscillatory myeloid cell recruitment pattern to atherosclerotic lesions regulated, in part, by rhythmic deposition of CCL2 on arterial endothelium. These findings lay the foundation for a chrono-pharmacological treatment strategy targeting early lesion development without disturbing microvascular recruitment of myeloid cells.


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Patients with Obesity Caused by Melanocortin-4 Receptor Mutations Can Be Treated with a Glucagon-like Peptide-1 Receptor Agonist

Publication date: Available online 31 May 2018
Source:Cell Metabolism
Author(s): Eva W. Iepsen, Jinyi Zhang, Henrik S. Thomsen, Elizaveta L. Hansen, Mette Hollensted, Sten Madsbad, Torben Hansen, Jens J. Holst, Jens-Christian Holm, Signe S. Torekov
Pathogenic mutations in the appetite-regulating melanocortin-4 receptor (MC4R) represent the most common cause of monogenic obesity with limited treatment options. Glucagon-like peptide-1 receptor agonists (GLP-1 RAs) cause weight loss by reducing appetite. We assessed the effect of the GLP-1 RA liraglutide 3.0 mg for 16 weeks in 14 obese individuals with pathogenic MC4R mutations (BMI 37.5 ± 6.8) and 28 matched control participants without MC4R mutation (BMI 36.8 ± 4.8). Liraglutide decreased body weight by 6.8 kg ± 1.8 kg in individuals with pathogenic MC4R mutations and by 6.1 kg ± 1.2 kg in control participants. Total body fat, waist circumference, and fasting and postprandial glucose concentrations similarly decreased in both groups. Thus, liraglutide induced an equal, clinically significant weight loss of 6% in both groups, indicating that the appetite-reducing effect of liraglutide is preserved in MC4R causal obesity and that liraglutide acts independently of the MC4R pathway. Thus, liraglutide could be an effective treatment of the most common form of monogenic obesity.

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Iepsen et al. show that the diabetes and obesity drug liraglutide, which has appetite-suppressing effects, caused weight loss in obese patients with mutations in the appetite-regulating melanocortin-4 receptor (MC4R). These results show that the appetite effects of liraglutide are independent of the MC4R pathway and offer therapeutic opportunities for patients with MC4R causal obesity.


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Cancer Lipid Metabolism Confers Antiangiogenic Drug Resistance

Publication date: Available online 31 May 2018
Source:Cell Metabolism
Author(s): Hideki Iwamoto, Mitsuhiko Abe, Yunlong Yang, Dongmei Cui, Takahiro Seki, Masaki Nakamura, Kayoko Hosaka, Sharon Lim, Jieyu Wu, Xingkang He, Xiaoting Sun, Yongtian Lu, Qingjun Zhou, Weiyun Shi, Takuji Torimura, Guohui Nie, Qi Li, Yihai Cao
Intrinsic and evasive antiangiogenic drug (AAD) resistance is frequently developed in cancer patients, and molecular mechanisms underlying AAD resistance remain largely unknown. Here we describe AAD-triggered, lipid-dependent metabolic reprogramming as an alternative mechanism of AAD resistance. Unexpectedly, tumor angiogenesis in adipose and non-adipose environments is equally sensitive to AAD treatment. AAD-treated tumors in adipose environment show accelerated growth rates in the presence of a minimal number of microvessels. Mechanistically, AAD-induced tumor hypoxia initiates the fatty acid oxidation metabolic reprogramming and increases uptake of free fatty acid (FFA) that stimulates cancer cell proliferation. Inhibition of carnitine palmitoyl transferase 1A (CPT1) significantly compromises the FFA-induced cell proliferation. Genetic and pharmacological loss of CPT1 function sensitizes AAD therapeutic efficacy and enhances its anti-tumor effects. Together, we propose an effective cancer therapy concept by combining drugs that target angiogenesis and lipid metabolism.

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Antiangiogenic drug (AAD) resistance is a frequent problem in cancer patients. Iwamoto et al. show that vascularization is not a limiting factor. Instead, AAD triggered a lipid-dependent metabolic reprogramming, resulting in increased free fatty acid and cancer cell proliferation. Inhibition of fatty acid oxidation enhanced the therapeutic efficacy of AAD.


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Control of Feeding Behavior by Cerebral Ventricular Volume Transmission of Melanin-Concentrating Hormone

Publication date: Available online 31 May 2018
Source:Cell Metabolism
Author(s): Emily E. Noble, Joel D. Hahn, Vaibhav R. Konanur, Ted M. Hsu, Stephen J. Page, Alyssa M. Cortella, Clarissa M. Liu, Monica Y. Song, Andrea N. Suarez, Caroline C. Szujewski, Danielle Rider, Jamie E. Clarke, Martin Darvas, Suzanne M. Appleyard, Scott E. Kanoski
Classical mechanisms through which brain-derived molecules influence behavior include neuronal synaptic communication and neuroendocrine signaling. Here we provide evidence for an alternative neural communication mechanism that is relevant for food intake control involving cerebroventricular volume transmission of the neuropeptide melanin-concentrating hormone (MCH). Results reveal that the cerebral ventricles receive input from approximately one-third of MCH-producing neurons. Moreover, MCH cerebrospinal fluid (CSF) levels increase prior to nocturnal feeding and following chemogenetic activation of MCH-producing neurons. Utilizing a dual viral vector approach, additional results reveal that selective activation of putative CSF-projecting MCH neurons increases food intake. In contrast, food intake was reduced following immunosequestration of MCH endogenously present in CSF, indicating that neuropeptide transmission through the cerebral ventricles is a physiologically relevant signaling pathway for energy balance control. Collectively these results suggest that neural-CSF volume transmission signaling may be a common neurobiological mechanism for the control of fundamental behaviors.

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Noble et al. identify a biological signaling mechanism whereby the neuropeptide melanin-concentrating hormone is transmitted via the brain cerebrospinal fluid (CSF) to increase feeding behavior. These findings suggest that neuropeptide transmission through the CSF may be an important signaling mechanism through which the brain regulates fundamental behaviors.


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Weight Gain and Impaired Glucose Metabolism in Women Are Predicted by Inefficient Subcutaneous Fat Cell Lipolysis

Publication date: Available online 31 May 2018
Source:Cell Metabolism
Author(s): Peter Arner, Daniel P. Andersson, Jesper Bäckdahl, Ingrid Dahlman, Mikael Rydén
Adipocyte mobilization of fatty acids (lipolysis) is instrumental for energy expenditure. Lipolysis displays both spontaneous (basal) and hormone-stimulated activity. It is unknown if lipolysis is important for future body weight gain and associated disturbed glucose metabolism, and this was presently investigated in subcutaneous adipocytes from two female cohorts before and after ≥10-year follow-up. High basal and low stimulated lipolysis at baseline predicted future weight gain (odds ratios ≥4.6) as well as development of insulin resistance and impaired fasting glucose/type 2 diabetes (odds ratios ≥3.2). At baseline, weight gainers displayed lower adipose expression of several established lipolysis-regulating genes. Thus, inefficient lipolysis (high basal/low stimulated) involving altered gene expression is linked to future weight gain and impaired glucose metabolism and may constitute a treatment target. Finally, low stimulated lipolysis could be accurately estimated in vivo by simple clinical/biochemical measures and may be used to identify risk individuals for intensified preventive measures.

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Although differences in energy metabolism may promote weight gain, the involved tissue(s) are unknown. Here, Arner et al. report that altered subcutaneous fat cell lipolysis is independently linked to long-term weight gain and disturbed glucose metabolism. This may depend on primary defects in the expression of specific lipolytic regulators.


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The SS18-SSX Fusion Oncoprotein Hijacks BAF Complex Targeting and Function to Drive Synovial Sarcoma

Publication date: Available online 31 May 2018
Source:Cancer Cell
Author(s): Matthew J. McBride, John L. Pulice, Hannah C. Beird, Davis R. Ingram, Andrew R. D'Avino, Jack F. Shern, Gregory W. Charville, Jason L. Hornick, Robert T. Nakayama, Enrique M. Garcia-Rivera, Dejka M. Araujo, Wei-Lien Wang, Jen-Wei Tsai, Michelle Yeagley, Andrew J. Wagner, P. Andrew Futreal, Javed Khan, Alexander J. Lazar, Cigall Kadoch
Synovial sarcoma (SS) is defined by the hallmark SS18-SSX fusion oncoprotein, which renders BAF complexes aberrant in two manners: gain of SSX to the SS18 subunit and concomitant loss of BAF47 subunit assembly. Here we demonstrate that SS18-SSX globally hijacks BAF complexes on chromatin to activate an SS transcriptional signature that we define using primary tumors and cell lines. Specifically, SS18-SSX retargets BAF complexes from enhancers to broad polycomb domains to oppose PRC2-mediated repression and activate bivalent genes. Upon suppression of SS18-SSX, reassembly of BAF47 restores enhancer activation, but is not required for proliferative arrest. These results establish a global hijacking mechanism for SS18-SSX on chromatin, and define the distinct contributions of two concurrent BAF complex perturbations.

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Incorporation of the synovial sarcoma SS18-SSX fusion into BAF complexes results in concomitant eviction of BAF47. McBride et al. show that SS18-SSX retargets BAF complexes from enhancers to polycomb domains to oppose PRC2-mediated repression. Reincorporation of BAF47 upon suppression of SS18-SSX restores enhancer activation but is not required for proliferative arrest.


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Cancer cachexia: Diagnosis, assessment, and treatment

Publication date: July 2018
Source:Critical Reviews in Oncology/Hematology, Volume 127
Author(s): Mohammadamin Sadeghi, Mahsa Keshavarz-Fathi, Vickie Baracos, Jann Arends, Maryam Mahmoudi, Nima Rezaei
Cancer cachexia is a multi-factorial syndrome, which negatively affects quality of life, responsiveness to chemotherapy, and survival in advanced cancer patients. Our understanding of cachexia has grown greatly in recent years and the roles of many tumor-derived and host-derived compounds have been elucidated as mediators of cancer cachexia. However, cancer cachexia remains an unmet medical need and attempts towards a standard treatment guideline have been unsuccessful. This review covers the diagnosis, assessment, and treatment of cancer cachexia; the elements impeding the formulation of a standard management guideline; and future directions of research for the improvement and standardization of current treatment procedures.



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Acoustic Field-Assisted Particle Patterning for Smart Polymer Composite Fabrication in Stereolithography

3D Printing and Additive Manufacturing, Ahead of Print.


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Editorial Board

Publication date: June–July 2018
Source:DNA Repair, Volumes 66–67





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Scholar : Experimental and Therapeutic Medicine - Volume:16 Number:1

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TABLE OF CONTENTS July-2018 Volume 16 Issue 1

Uridine 5'‑diphospho‑glucronosyltrasferase: Its role in pharmacogenomics and human disease (Review) Celia N. Sanchez‑Dominguez, Hugo L. Gallardo‑Blanco, Mauricio A. Salinas‑Santander, Rocio Ortiz‑Lopez View Abstract ❯
IL‑17A and GDF15 are able to induce epithelial‑mesenchymal transition of lung epithelial cells in response to cigarette smoke Gang Jiang, Chen‑Tao Liu, Wei‑Dong Zhang View Abstract ❯
Nicotine reduces effectiveness of doxorubicin chemotherapy and promotes CD44+CD24‑ cancer stem cells in MCF‑7 cell populations Leyla Türker Şener, Celal Güven, Aziz Şener, Suzan Adin Çinar, Seyhun Solakoğlu, Işil Albeniz View Abstract ❯
Protective effects of nicorandil against cerebral injury in a swine cardiac arrest model Fangfang Zhu, Xia Zhong, Yi Zhou, Zhiqiang Hou, Haoran Hu, Lining Liang, Jibin Chen, Qianqian Chen, Xianfei Ji, Deya Shang View Abstract ❯
MicroRNA‑150 suppresses the growth and malignant behavior of papillary thyroid carcinoma cells via downregulation of MUC4 Zhenzhong Fa, Zhenyu Min, Jianjun Tang, Chuanlei Liu, Guodu Yan, Jianbo Xi View Abstract ❯
Expression of Notch receptors and their ligands in pancreatic ductal adenocarcinoma Hai‑Yan Song, Ying Wang, Hong Lan, Yu‑Xiang Zhang View Abstract ❯
Smoking and drinking influence the advancing of ischemic stroke disease by targeting PTGS2 and TNFAIP3 Zhimin Miao, Meifang Guo, Suqin Zhou, Xuemei Sun, Fang Wang, Haiying Lu, Zhenhong Cui View Abstract ❯
Experimental research on the effect of microRNA‑21 inhibitor on a rat model of intervertebral disc degeneration Xiaoming Sheng, Qingsong Guo, Junbo Yu, Youjia Xu View Abstract ❯
Long non‑coding RNA GAS5 inhibits ovarian cancer cell proliferation via the control of microRNA‑21 and SPRY2 expression Nana Ma, Shaoru Li, Quanhua Zhang, Hongmei Wang, Haixia Qin, Shijin Wang View Abstract ❯
Propofol improves the function of natural killer cells from the peripheral blood of patients with esophageal squamous cell carcinoma Min Zhou, Junchao Dai, Yu Zhou, Jian Wu, Tao Xu, Denglian Zhou, Xiaobin Wang View Abstract ❯
Establishment of a novel rat model of blast‑related diffuse axonal injury Jun‑Hai Zhang, Jian‑Wen Gu, Bing‑Cang Li, Fa‑Bao Gao, Xiao‑Ming Liao, Shao‑Jie Cui View Abstract ❯
S‑adenosyl methionine regulates calcium channels and inhibits uterine smooth muscle contraction in rats with infectious premature delivery through the transient receptor protein 3/protein kinase Cβ/C‑kinase‑activated protein phosphatase‑1 inhibitor of 17 kDa signaling pathway Jing Ge, Tao Han, Xiaoqiu Li, Lili Shan, Jinhuan Zhang, Yan Hong, Yanqiu Xia, Jun Wang, Mingxiao Hou View Abstract ❯
A cynomolgus monkey model of carotid atherosclerosis induced by puncturing and scratching of the carotid artery combined with a high‑fat diet Lei Zhang, Yan Zeng, Ji Qi, Yanxiao Xu, Shaoqun Zhang, Xin Zhou, Ruiyue Ping, Shijie Fu View Abstract ❯
Identification of genes and pathways related with cerebral small vessel disease based on a long non-coding RNA-mediated, competitive endogenous RNA network Hui Yan, Xiaoli Yang, Xueman Zhao, Huankun Wang View Abstract ❯
Relationship between changes in mitochondrial function and hippocampal neuronal apoptosis after recurrent convulsion during developmental stage Yueying Liu, Jieru Chen, Meifang Jin, Zhenhong Li, Tian Tian, Lili Li, Hong Ni View Abstract ❯
FOXA2 promotes the proliferation, migration and invasion, and epithelial mesenchymal transition in colon cancer Baolei Wang, Guangwei Liu, Lei Ding, Jun Zhao, Yun Lu View Abstract ❯
Early growth response factor‑1 DNA enzyme 1 inhibits the formation of abdominal aortic aneurysm in rats Shi Wang, Haipeng Dong, Chengwei Liu, Guichao Xu, Xinhua Hu, Yichuan Fan, Liting Chen View Abstract ❯
Identification of differential modules in ankylosing spondylitis using systemic module inference and the attract method Fang‑Chang Yuan, Bo Li, Li‑Jun Zhang View Abstract ❯
Inhibition of prostaglandin E2 protects abdominal aortic aneurysm from expansion through regulating miR-29b-mediated fibrotic ECM expression Zonglin Han, Tangshan Zhang, Yuxiang He, Gang Li, Gang Li, Xing Jin View Abstract ❯
Curative effect of posterior lumbar interbody fusion in the treatment of single-segment lumbar degenerative disease and changes in adjacent segment quantitative score Yan Zhuang, Feng Zhou, Yunqin Zhang, Zheng Jin View Abstract ❯
Effects of compound Danshen injection combined with magnesium sulfate on serum MPO and hs-CRP in patients with severe preeclampsia Kun Yang, Gaoxia Dong, Ying Tian, Jian Li View Abstract ❯
Effect of interleukin‑31 on septic shock through regulating inflammasomes and interleukin‑1β Xuyun Gu, Chen Wei, Xishan Zhu, Feiping Lu, Bo Sheng, Xuefeng Zang View Abstract ❯
TGF‑β/Smad3 pathway enhances the cardio‑protection of S1R/SIPR1 in in vitro ischemia‑reperfusion myocardial cell model Tingfang Yang, Xianfeng Zhang, Cuimei Ma, Yan Chen View Abstract ❯
Hematopoietically expressed homeobox gene is associated with type 2 diabetes in KK Cg‑Ay/J mice and a Taiwanese Han Chinese population Chi‑Cheng Lu, Yng‑Tay Chen, Shih‑Yin Chen, Yuan‑Man Hsu, Chyi‑Chyang Lin, Je‑Wei Tsao, Yu‑Ning Juan, Jai‑Sing Yang, Fuu‑Jen Tsai View Abstract ❯
Mechanism of melatonin combined with calcium carbonate on improving osteoporosis in aged rats Fuqiang Zhu, Zhendong Liu, Yuxin Ren View Abstract ❯
Effects of forkhead box protein M1 on trophoblast invasion and its role in preeclampsia development Xuena Cui, Jin'e Xu, Yuzhi Ji, Xiuhong Song, Junhuan Wang, Lijuan Zhang, Shengmei Yang, Yuanhua Ye View Abstract ❯
Dendrobium mixture regulates hepatic gluconeogenesis in diabetic rats via the phosphoinositide‑3‑kinase/protein kinase B signaling pathway Xinjun Lin, Hong Shi, Yi Cui, Xiaoning Wang, Jieping Zhang, Wenzhen Yu, Min Wei View Abstract ❯
Expression profiling of lipocalin‑2 and 24p3 receptor in murine gonads at different developmental stages Elsa De La Chesnaye, Leticia Manuel‑Apolinar, Leticia Damasio, Aleida Olivares, Miguel Angel Palomino, Isis Santos, Juan Pablo Méndez View Abstract ❯
High glucose induces epithelial‑mesenchymal transition and results in the migration and invasion of colorectal cancer cells Jiayan Wu, Jiayi Chen, Yang Xi, Fuyan Wang, Hongcun Sha, Lin Luo, Yabin Zhu, Xiaoming Hong, Shizhong Bu View Abstract ❯
Mental and physical stress of the Fukushima disaster evacuees as estimated by the measurement of urinary 8‑hydroxy‑2'‑deoxyguanosine Yasuyo Fukushi, Ayumi Nakamura, Chieko Itaki, Shinji Tokonami, Masatoshi Yamada, Yasushi Mariya View Abstract ❯
Glycerol fructose combined with vitamin B6 is beneficial to postoperative recovery of patients with cerebral aneurysm Xiya Chen, Ting Lei View Abstract ❯
Serum STLT-1 and bilirubin levels in patients with acute coronary syndrome and correlation with prognosis Rong Fu, Xu Song, Dexing Su, Shunrong Li, Lizhen Gao, Chunmei Ji View Abstract ❯
Changes of lysosomal membrane permeabilization and lipid metabolism in sidt2 deficient mice Yu Meng, Lizhuo Wang, Liefeng Ling View Abstract ❯
Automatic tube potential selection with tube current modulation in coronary CT angiography: Can it achieve consistent image quality among various individuals? Xiao‑Ping Wang, Xiao‑Mei Zhu, Yin‑Su Zhu, Wang‑Yan Liu, Xiao‑Han Yang, Wei‑Wei Huang, Yi Xu, Li‑Jun Tang View Abstract ❯
Randomized clinical trial of physiological ischemic training for patients with coronary heart disease complicated with heart failure: Safety of training, VEGF of peripheral blood and quality of life Min Gao, Xiao Lu, Wei Chen, Gui‑Hong Xiao, Yijin Zhang, Rongbin Yu, Jianan Li View Abstract ❯
A study of the correlation of insulin resistance and leptin with inflammatory factors and vascular endothelial injury in T2DM patients with CHD Jie Zhang, Jing Jin, Jilun Liu, Yajun He, Peng Zhang, Wucheng Ye, Wei Zhu, Mingliang Li View Abstract ❯
The effect of nitric oxide inhalation on heart and pulmonary circulation in rabbits with acute massive pulmonary embolism Zeming Zhang, Kun Pan, Lu Chen, Yancun Wang View Abstract ❯
Diagnostic value of detection of serum β‑HCG and CT‑IgG combined with transvaginal ultrasonography in early tubal pregnancy Hongyan Xin, Wenlian Liu, Ping Li View Abstract ❯
Overexpression of indoleamine 2, 3‑dioxygenase contributes to the repair of human airway epithelial cells inhibited by dexamethasone via affecting the MAPK/ERK signaling pathway Shanshan Jia, Pin Guo, Xiangjin Ge, Huanhuan Wu, Junhua Lu, Xiaofang Fan View Abstract ❯
Heme oxygenase‑1 exerts pro‑apoptotic effects on hepatic stellate cells in vitro through regulation of nuclear factor‑κB Hui Yang, Bangtao Chen, Zhongfu Zhao, Li Zhang, Yun Zhang, Jie Chen, Xiaoqian Zhang, Xiaohua Zhang, Longfeng Zhao View Abstract ❯
Effects of Kangfuxin solution on IL‑1β, IL‑6, IL‑17 and TNF‑α in gingival crevicular fluid in patients with fixed orthodontic gingivitis Yuting Liu, Fengping Mu, Lijuan Liu, Chune Shan View Abstract ❯
Molecular mechanism of the role of carbamyl erythropoietin in treating diabetic retinopathy rats Xuegu Xu, Yonghao Cai, Yinfei Yu View Abstract ❯
Therapeutic effects of acupuncture with MOK, a polyherbal medicine, on PTU‑induced hypothyroidism in rats Ji Hye Hwang, Hyo Won Jung, Seok Yong Kang, An Na Kang, Jun Nan Ma, Xiang Long Meng, Min Sub Hwang, Yong‑Ki Park View Abstract ❯
Early enteral nutrition and total parenteral nutrition on the nutritional status and blood glucose in patients with gastric cancer complicated with diabetes mellitus after radical gastrectomy Junli Wang, Jiamin Zhao, Yanling Zhang, Chong Liu View Abstract ❯
Differential network as an indicator of osteoporosis with network entropy Lili Ma, Hongmei Du, Guangdong Chen View Abstract ❯
Resveratrol provides benefits in mice with type II diabetes‑induced chronic renal failure through AMPK signaling pathway Haiyan Guo, Linyun Zhang View Abstract ❯
Unilateral digital arterial ligation combined with low molecular weight heparins in severed finger without venous anastomosis Xueming Chen, Zheng Chen, Jiandong Zhou, Yajun Xu View Abstract ❯
Ellagic acid promotes A549 cell apoptosis via regulating the phosphoinositide 3‑kinase/protein kinase B pathway Qiong Liu, Xiaobing Liang, Chengwei Niu, Xuelan Wang View Abstract ❯
Electro‑acupuncture stimulation prevents remifentanil‑induced postoperative hyperalgesia by suppressing spinal microglia in rats Yanhu Xie, Jun Ma, Di Wang, Xiaoqing Chai , Chen Gao View Abstract ❯
Long‑term survival in a recipient of kidney transplant without maintenance immunosuppression: A case report Sujuan Feng, Yuan Zhuang, Hang Liu, Xiaodong Zhang View Abstract ❯
Comparison of the clinical effects of arthroscopic surgery vs. open surgery for grade II gluteal muscle contracture in adults Ting Zhang, Siyue Xu, Haopeng Li, Xijing He, Feng Zhang View Abstract ❯
Nimesulide inhibits proliferation and induces apoptosis of pancreatic cancer cells by enhancing expression of PTEN Meifen Chu, Tongtong Wang, Aihua Sun, Yu Chen View Abstract ❯
Effects of hyperglycaemia and elevated glycosylated haemoglobin on contrast‑induced nephropathy after coronary angiography Yu‑Han Qin, Gao‑Liang Yan, Chang‑Le Ma, Cheng‑Chun Tang, Gen‑Shan Ma View Abstract ❯
Knockdown of RWD domain containing 3 inhibits the malignant phenotypes of glioblastoma cells via inhibition of phosphoinositide 3‑kinase/protein kinase B signaling Xiaofeng Chen, Weiping Kuang, Hongxing Huang, Bo Li, Yong Zhu, Bin Zhou, Lin Yan View Abstract ❯
Comparison of efficacy, complications and TGF‑β2 expression between DHS and PFNA in elderly patients with osteoporotic femoral intertrochanteric fracture Rao Xu, Jiangying Ru, Feng Ji, Jie Liu, Yong Ji, Zhiquan Wu, Dai Shi View Abstract ❯
Anti‑inflammatory effect of polydeoxyribonucleotide on zoledronic acid‑pretreated and lipopolysaccharide‑stimulated RAW 264.7 cells Jin‑Hee Han, Junho Jung, Lakkyong Hwang, Il‑Gyu Ko, Ok Hyung Nam, Mi Sun Kim, Jung‑Woo Lee, Byung‑Joon Choi, Deok‑Won Lee View Abstract ❯
Lung injury caused by paraquat poisoning results in increased interleukin‑6 and decreased microRNA‑146a levels Wei Wu, Yong Li View Abstract ❯
Thoracic manifestation of Wegener's granulomatosis: Computed tomography findings and analysis of misdiagnosis Jiakai Li, Chuangui Li, Jiaojiao Li View Abstract ❯
Beneficial effects of dexmedetomidine on early postoperative cognitive dysfunction in pediatric patients with tonsillectomy Chuanlai Han, Rong Fu, Weifu Lei View Abstract ❯
Maternal lipids, BMI and IL‑17/IL‑35 imbalance in concurrent gestational diabetes mellitus and preeclampsia Weiping Cao, Xinzhi Wang, Tingmei Chen, Wenlin Xu, Fan Feng, Songlan Zhao, Zuxian Wang, Yu Hu, Bing Xie View Abstract ❯
miR‑199b‑5p serves as a tumor suppressor in renal cell carcinoma Yulin Lai, Jing Quan, Jia Hu, Peijie Chen, Jinling Xu, Xin Guan, Weijie Xu, Yongqing Lai, Liangchao Ni View Abstract ❯
Protective effect of Fructus corni polysaccharide on hippocampal tissues and its relevant mechanism in epileptic rats induced by lithium chloride‑pilocarpine Xiaomin Sun, Lingting Kong, Li Zhou View Abstract ❯
MicroRNA‑27a protects retinal pigment epithelial cells under high glucose conditions by targeting TLR4 Xiaolei Tang, Yan Dai, Xiaoli Wang, Jian Zeng, Guirong Li View Abstract ❯
[Retracted] Recent perspectives of pediatric mitochondrial diseases Junhua Cao, Hongwei Wu, Zhenguang Li View Abstract ❯

20-22 September, 2018, Metropolitan Hotel, Athens, Greece 23nd World Congress on Advances in Oncology & 22th International Symposium on Molecular Medicine 18-19 September 2018, Pre-Congress Workshop: 'MicroRNA Analysis'

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