Abstract
Objective
The objective of this study was to evaluate the effect of a Biosilicate®, associated with dentin adhesive system, on microtensile bond strength (μTBS) to sound and demineralized dentin.
Materials and methods
Eighty sound-extracted molars had their middle occlusal dentin exposed. In forty teeth, dentin was artificially demineralized (pH cycling). Sound and demineralized dentin teeth were separated into four groups (n = 10), according to the substrate treatment before restoration: Group 1—total-etching adhesive Adper TM Single bond 2 (ASB) + Biosilicate®, Group 2—ASB (without Biosilicate®), Group 3—AdheSE self-etching adhesive system (AdSE) + Biosilicate®, and Group 4—AdSE (without Biosilicate®). Each tooth was restored with a hybrid composite and stored in water at 37 °C for 6 months. After water aging, teeth were cut in sticks (≈ 1 mm2 cross-sectional area) and all samples were submitted to μTBS test. The fracture modes of the samples were analyzed by stereomicroscopy. The representatively fractured samples were observed by scanning electron microscopy. Representative samples of each group were analyzed on energy dispersive X-ray spectrometry (EDX). The μTBS and Ca-P ratio values were analyzed by 2-way ANOVA, Bonferroni, and Tukey test, respectively, p < .05.
Results
ASB + Biosilicate® presented the highest μTBS values (p < .05), and lowest μTBS values (p < .05) were found in AdSE Group. There was no statistical difference (p < .05) on μTBS when substrates were compared, except for Group 2. The fracture pattern analysis showed prevalence of adhesive fractures in all groups.
Conclusion
Biosilicate® enhanced bond strength of self-etching and etch-and-rinse adhesives to sound and demineralized dentin.
Clinical relevance
Bioactive glass ceramic suspension could be recommended to be used to improve the dentin bond strengths of the total-etching and self-etching adhesives after acid-etching and priming.
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