An overview of the infection of CMV, HSV 1/2 and EBV in Mexican patients with glioblastoma multiforme

Publication date: Available online 24 December 2016
Source:Pathology - Research and Practice
Author(s): Zavala-Vega Sergio, Castro-Escarpulli Graciela, Hernández-Santos Hector, Salinas-Lara Citlatepetl, Palma Icela, Mejía-Aranguré Juan Manuel, Gelista-Herrera Noemí, Rembao-Bojorquez Daniel, Sara A. Ochoa, Cruz-Córdova Ariadnna, Xicothencatl-Cortes Juan, Uribe-Gutiérrez Gabriel, Arellano-Galindo José
Several risk factors are involved in glioblastoma, including cytomegalovirus (CMV). This research was carried out to determine the rate of CMV infection, as well as HSV 1/2 and EBV in brain tissue, in patients with glioblastomamultiforme (GBM). The tissues were tested using immunohistochemistry, PCR, in situ hybridization and real-time PCR. At least, one HHV was detected in 21/29 (72%) patients as follows: single infections with HSV-1/2 in 4/21 (19%), EBV in 6/21 (28.6%) and CMV in 1/21 (4.8%). Mixed viral infection, HSV-1/2 and EBV were detected in 4/21 patients (19%), CMV and EBV in 5/21 (23.8%), and HSV-1/2, EBV, and CMV in 1/21. The CMV viral load ranged from 3×10² to 4.33×10⁵ genome/100ng of tissue. Genotype based on CMV gB was 3/7 where 2/3 was gB1 and 1/3 gB4). HSV, EBV and CMV were frequently found in brain tissues, more in mix in a population reported as highly seropositive.



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