Cadmium adsorption, chelation and compartmentalization limit root-to-shoot translocation of cadmium in rice ( Oryza sativa L.)

Abstract

Strategies to reduce cadmium (Cd) in rice grain, below concentrations that represent serious human health concerns, require that the mechanisms of Cd distribution and accumulation within rice plants be established. Here, a comprehensive hydroponic experiment was performed to investigate the differences in the Cd uptake, chelation and compartmentalization between high (D83B) and low (D62B) Cd-accumulation cultivars contrasting in Cd accumulation in order to establish the roles of these processes in limiting Cd translocation from root to shoot. D83B showed 3-fold higher Cd accumulation in the shoots than the cultivar D62B. However, a short-term Cd uptake experiment showed more Cd uptake by D62B than by D83B. The distribution of Cd in roots and shoots differed significantly. D83B translocated 38% of total Cd taken up to the shoots, whereas D62B retained most of the Cd in the roots. D62B had higher amounts of non-protein thiols (NPTs) and glutathione (GSH) than D83B. The NPT and Cd distribution ratio (CDR) in the anionic form in the roots of D62B increased gradually as Cd concentration increased. In D83B, in contrast, levels of CDR in the cationic form increased significantly from 22.10 to 43.37%, while NPT only increased slightly. Furthermore, the percentage of Cd ions retained in thiol-rich peptides, especially in the HMW complexes, was significantly higher in D62B compared with D83B. However, D83B possessed a greater proportion of potentially mobile (cationic) Cd in the roots and showed superior Cd translocation from root to shoot. Taken as a whole, the results presented in this study revealed that Cd chelation, compartmentalization and adsorption contribute to the Cd retention in roots.

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