Comparison of photoinactivation of T. rubrum by new methylene blue (NMB) and indocyanine green (EmunDo®)

Publication date: June 2017
Source:Photodiagnosis and Photodynamic Therapy, Volume 18
Author(s): Reza Fekrazad, Arash Poorsattar Bejeh Mir, Mina Kahyaie Aghdam, Vadood Ghasemi Barghi
BackgroundSuperficial mycotic skin infections which are predominantly caused by Trichophyton rubrum, poorly responsd to conventional therapies. A great amount of attention has focused on finding more effective treatments. The current work is aimed to compare the effectiveness of phoinactivation of Trichophyton rubrum by two relatively new photosensitizers: a phenothiazinium dye(New methylene blue) and Indocyanine green (EmunDo^®).Materials and methodsA Final inoculum of T. rubrum which corresponded to 10⁶ colony forming unit per milliliter (CFUml⁻¹) was prepared. Antimicrobial Photodynamic treatment (aPDT) of T. rubrum was carried out by either EmunDo^® (1mg/ml, Infra-red laser (IRL, λ=810nm, Energy Density 55J/cm²)) or NMB (10μM, Red laser (RL), λ=630nm, Energy Density of 5J/cm²). The suspensions thereafter were subcultured on Sabouraud dextrose agar (SDA) and were counted on due time. based on colony-forming unit per milliliter (CFU/ml).ResultsaPDT with either EmunDo^® (E) or NMB (N) considerably diminished the viability of inoculated T. rubrum with respective reduction of 0.64 log and 0.4 log compared to the control group (P<0.001). No significant difference was found between two laser only groups (P=0.79) and two aPDT groups (P=0.73), however significant reduction of T. rubrum in red laser only group (P=0.04) and EmunDo^® only group (P=0.04) was found as compared to the control group (P<0.05).ConclusionThe study provides evidence regarding satisfactory photodynamic inactivation of T. rubrum with EmunDo^® or NMB as photosensitizers. Irradiation by only red laser source was found superior to only infra-red laser source. Dark toxicity of EmunDo^® was more successful than new methylene blue dye.



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