Antimicrobial photodynamic therapy with photosensitizer in ethanol improves oxidative status and gingival collagen in a short-term in periodontitis

Publication date: Available online 12 May 2017
Source:Photodiagnosis and Photodynamic Therapy
Author(s): Fernanda Maia Pillusky, Raquel Cristine Silva Barcelos, Luciana Taschetto Vey, Luisa Machado Barin, Victor de Mello Palma, Roberto Marinho Maciel, Karla Zanini Kantorski, Marilise Escobar Bürger, Cristiane Cademartori Danesi
BackgroundThis study evaluated the antimicrobial photodynamic therapy (aPDT) effects using the methylene blue (MB) in ethanol 20% on systemic oxidative status and collagen content from gingiva of rats with periodontitis.MethodsRats were divided into five experimental groups: NC (negative control; no periodontitis); PC (positive control; periodontitis without any treatment); SRP (periodontitis and scaling and root planing), aPDT I (periodontitis and SRP+aPDT+MB solubilized in water), and aPDT II (periodontitis and SRP+aPDT+MB solubilized in ethanol 20%). After 7days of removal of the ligature, the periodontal treatments were performed. At 7/15/30 days, gingival tissue was removed for morphometric analysis. The erythrocytes were used to evaluate systemic oxidative status.ResultsPC group showed higher lipoperoxidation levels at 7/15/30 days. aPDT indicated a protective influence in erythrocytes at 15days observed by the elevation in levels of systemic antioxidant defense. aPDT II group was the only one that restored the total collagen area in 15 days, and recovered the type I collagen area at the same time point.ConclusionsaPDT as an adjunct to the SRP can induce the systemic protective response against oxidative stress periodontitis-induced and recover the gingival collagen, thus promoting the healing periodontal, particularly when the MB is dissolved in ethanol 20%.

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